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The map label for this gene is hisH [H]

Identifier: 226948900

GI number: 226948900

Start: 1841724

End: 1842329

Strand: Direct

Name: hisH [H]

Synonym: CLM_1810

Alternate gene names: 226948900

Gene position: 1841724-1842329 (Clockwise)

Preceding gene: 226948899

Following gene: 226948901

Centisome position: 44.32

GC content: 30.03

Gene sequence:

>606_bases
GTGATTTCAATTGTGGATTATGGTATGGGAAATTTAAAAAGTGTAGAAAATGCACTTGATTTTTTAGGTATAAAGTCTGT
AATTACATCAGAGAGAGAAGTAATTTTAAATAGCGATGGAATAATATTACCAGGAGTGGGAGCTTTTCCAGATGCCATTA
ATAACATTAAAAGAGAGGGTATTGATAAAGTTTTAAAGGAAGCAGTAAAAAAGGATAAACCTTTACTTGGAATATGTCTT
GGAATGCAGCTTTTATTTGAAGAAAGTGAAGAAATTAAAAGCTATAGAGGATTAGGTTTTTTAAAAGGAAAAATAGAAAA
AATGAAGGTAAATTTAAAAATACCTCATATGGGCTGGAATAATTTAAGTTTTTGTAAAGATTCCCCAATACTTAAGGGGA
TAAAACAGAGGAGTTATGTGTATTATGTTCACTCTTATTATGCAAAAATTGAAGAGGAAGGAATATTAAATGCCTATTCC
CAATATGGAATAGAGGTACCGGGGATAGTAAGTAAAAATAATATTTATGGAATACAGTTTCATCCTGAAAAAAGTGGGGA
TATAGGACTCAAAATATTAAGTAATTTTGGGGAGCTGATAAAATGA

Upstream 100 bases:

>100_bases
ACAATTATGTTTATTATTAAATATTACAAGCTTTACAATGAGTAATAGGGGATATATAAAGCCAATAACATTAAAAAAGA
AATATAAGGGGGACAAATAG

Downstream 100 bases:

>100_bases
TAATTTTACCTGCCATAGATTTAAAAGAGGGTAAATGTATTAGATTATACCAAGGAGATTTTAAAGCTTCAAAGGTGGTA
GCAGAAGATCCTATAGAGGT

Product: imidazole glycerol phosphate synthase subunit HisH

Products: NA

Alternate protein names: IGP synthase glutamine amidotransferase subunit; IGP synthase subunit hisH; ImGP synthase subunit hisH; IGPS subunit hisH [H]

Number of amino acids: Translated: 201; Mature: 201

Protein sequence:

>201_residues
MISIVDYGMGNLKSVENALDFLGIKSVITSEREVILNSDGIILPGVGAFPDAINNIKREGIDKVLKEAVKKDKPLLGICL
GMQLLFEESEEIKSYRGLGFLKGKIEKMKVNLKIPHMGWNNLSFCKDSPILKGIKQRSYVYYVHSYYAKIEEEGILNAYS
QYGIEVPGIVSKNNIYGIQFHPEKSGDIGLKILSNFGELIK

Sequences:

>Translated_201_residues
MISIVDYGMGNLKSVENALDFLGIKSVITSEREVILNSDGIILPGVGAFPDAINNIKREGIDKVLKEAVKKDKPLLGICL
GMQLLFEESEEIKSYRGLGFLKGKIEKMKVNLKIPHMGWNNLSFCKDSPILKGIKQRSYVYYVHSYYAKIEEEGILNAYS
QYGIEVPGIVSKNNIYGIQFHPEKSGDIGLKILSNFGELIK
>Mature_201_residues
MISIVDYGMGNLKSVENALDFLGIKSVITSEREVILNSDGIILPGVGAFPDAINNIKREGIDKVLKEAVKKDKPLLGICL
GMQLLFEESEEIKSYRGLGFLKGKIEKMKVNLKIPHMGWNNLSFCKDSPILKGIKQRSYVYYVHSYYAKIEEEGILNAYS
QYGIEVPGIVSKNNIYGIQFHPEKSGDIGLKILSNFGELIK

Specific function: IGPS catalyzes the conversion of PRFAR and glutamine to IGP, AICAR and glutamate. The hisH subunit provides the glutamine amidotransferase activity that produces the ammonia necessary to hisF for the synthesis of IGP and AICAR [H]

COG id: COG0118

COG function: function code E; Glutamine amidotransferase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 glutamine amidotransferase type-1 domain [H]

Homologues:

Organism=Escherichia coli, GI1788334, Length=199, Percent_Identity=37.1859296482412, Blast_Score=135, Evalue=2e-33,
Organism=Saccharomyces cerevisiae, GI6319725, Length=208, Percent_Identity=33.6538461538462, Blast_Score=115, Evalue=3e-27,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR017926
- InterPro:   IPR000991
- InterPro:   IPR010139
- InterPro:   IPR016226 [H]

Pfam domain/function: PF00117 GATase [H]

EC number: 2.4.2.-

Molecular weight: Translated: 22447; Mature: 22447

Theoretical pI: Translated: 8.59; Mature: 8.59

Prosite motif: PS00442 GATASE_TYPE_I

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
2.5 %Met     (Translated Protein)
3.5 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
2.5 %Met     (Mature Protein)
3.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MISIVDYGMGNLKSVENALDFLGIKSVITSEREVILNSDGIILPGVGAFPDAINNIKREG
CEEEEECCCCCHHHHHHHHHHHHHHHHHCCCCEEEECCCCEEECCCCCCHHHHHHHHHHH
IDKVLKEAVKKDKPLLGICLGMQLLFEESEEIKSYRGLGFLKGKIEKMKVNLKIPHMGWN
HHHHHHHHHHHCCCEEHHHHHHHHHHHHHHHHHHHCCCHHHHCEEEEEEEEEEECCCCCC
NLSFCKDSPILKGIKQRSYVYYVHSYYAKIEEEGILNAYSQYGIEVPGIVSKNNIYGIQF
CCCCCCCCHHHHHHHHCCEEEEHHHHHHHHHHCHHHHHHHHCCCCCCCEEECCCEEEEEE
HPEKSGDIGLKILSNFGELIK
CCCCCCCHHHHHHHHHHHHCC
>Mature Secondary Structure
MISIVDYGMGNLKSVENALDFLGIKSVITSEREVILNSDGIILPGVGAFPDAINNIKREG
CEEEEECCCCCHHHHHHHHHHHHHHHHHCCCCEEEECCCCEEECCCCCCHHHHHHHHHHH
IDKVLKEAVKKDKPLLGICLGMQLLFEESEEIKSYRGLGFLKGKIEKMKVNLKIPHMGWN
HHHHHHHHHHHCCCEEHHHHHHHHHHHHHHHHHHHCCCHHHHCEEEEEEEEEEECCCCCC
NLSFCKDSPILKGIKQRSYVYYVHSYYAKIEEEGILNAYSQYGIEVPGIVSKNNIYGIQF
CCCCCCCCHHHHHHHHCCEEEEHHHHHHHHHHCHHHHHHHHCCCCCCCEEECCCEEEEEE
HPEKSGDIGLKILSNFGELIK
CCCCCCCHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: Transferases; Glycosyltransferases; Pentosyltransferases [C]

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11466286 [H]