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The map label for this gene is appA [H]

Identifier: 226948494

GI number: 226948494

Start: 1443966

End: 1445564

Strand: Reverse

Name: appA [H]

Synonym: CLM_1383

Alternate gene names: 226948494

Gene position: 1445564-1443966 (Counterclockwise)

Preceding gene: 226948501

Following gene: 226948493

Centisome position: 34.79

GC content: 29.89

Gene sequence:

>1599_bases
ATGAAAGTGAAGAAACTATTAGCCATGATATTAGTAGCCTCAACTACCTTAGTGGCCACTGCTTGTGGAAATAGTAATAG
TTCTAGCGGATCTACATCCAAAGAAACTTCCGCAAAAGAAAATATTAAAGATGGAGGAAATCTAGTATTTTCTATCCGTG
GTGAGCCAGAAATACTTAATCCAATCTATGCTTACGACAGAGATACTATGACTATGGATAATGCTTTATTTGCACCTTTA
TTTTATATGAATGGAGATAAAATTGATTACACTCTAGCTGAAGAAGTAAAGCATTCAGATGATTTTTTAACTTATACAGT
AAAATTAAAAAAAGATTTAAAATGGCATGATGGTAAACCTTTAACTGCTGATGATTTAGTATTTACTATGAAACAAATAA
TGGATGAAAAACAAGATTCACCTTTTAGAAGTGCCTTTGTTATTAATGATAAACCTGTAGAGGTAAAAAAGGTAGATGAC
TTAACCGTAGAGTTTAAACTACCAACAGTTCAAATGCCTTTTATGAATTCTTTAGGACAAGTATCTCCTATTCCTAAACA
TGTATTTGAAGGAGAAAAAGATATTAAAAAAAGTGCTAAAAATGAAAAACCTATAGGTTCTGGTGCCTTTAAATTTAGAG
AATCTAAAAAAGGTGAAAGTATAACGCTAGAAAGATTCGATAACTATGTTGGTGGAAAACCTCATCTTGACACAATAACC
TATAGAATAATTGCTGATCCAAATTCATCTAAGGTAGCTCTTGAAAATGGCGAAGTTTCTGCTAATTATATAGATATTAG
CGGTATAAGTAAATTTGAAAAAAATGAAAAATTAAAAGTAGTTGCCTATGATGAAGGTATGGTGGATAATTTAGTCTTAA
ATTGTAAAGCAAAAGGTTTAGATAAAAAAGAAGTTAGACAAGCTATAGCCTATGCTTTAAATAAAGATGATTTAATAAAG
GCTGCTTATGAAAGTGAAAAATATGCCCCTAAGGCCTATTCCCCACTACCTAAGAATGCCTTATATTATACAGAGGATGT
TACTAAATATGACTTAAATAAAGATAAAGCAAAGGAATTACTAAAAAAATCCGGTGCTGAAAATTTAAAACTTAAGCTAG
TTTATAGAAATGATAAAAAGACTTTAGAAAATCAAGCTTTAGTAGTTAAAGAAAATTTAAAAGATATAGGAATAGATGTT
GAATTAAAGGGATTAGAAGCAAATGCCTTTTTTAAGCAAATCGATGATCCTTCTAAAGCTGATTTTGACTTGATATTTAA
TGCCTATTTAATGGGTAACGAGCCTGATGCTTATAAAGAAGTATTTATGACAAACGGAGCTTTTAACGCTTCTAGATATA
ATAATAAAAAACTAGATGACCTTTGGAATAAAGCTGCCGTAGAAACTGATAAAACAAAGAGAGAAGAAATTTATAAAACT
ATTCAAAAGGAACTTATAGAAGATATGCCTGTATACCCAATTTGCTATTCTAATGCCACAATAGCAGTAAATAAAAATGT
AGGAGGTATAAAAGAAGCAAAAACAGCTCCTATATATATGTTCCAAGACTTATCAAAACTTTATATGATAGAAAAATAA

Upstream 100 bases:

>100_bases
CTATGATTTACTTTTATTATTTAACAATTTTTTATTAAAATGGTATCATTTATTAGTAGAATGAGTAGATTAATTTTTAC
TATTAGAAAGGGGTTTTTTT

Downstream 100 bases:

>100_bases
AAATTTAATAGTTAAAAGGACGGTATTAATTGGATTACCCTTTTGATACCGTTTATTTTTAAATAAAACAAGTACTTTAA
ACTTTTATAATATTTATTTA

Product: putative oligopeptide ABC transporter oligopeptide-binding protein

Products: ADP; phosphate; dipeptides [Cytoplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 532; Mature: 532

Protein sequence:

>532_residues
MKVKKLLAMILVASTTLVATACGNSNSSSGSTSKETSAKENIKDGGNLVFSIRGEPEILNPIYAYDRDTMTMDNALFAPL
FYMNGDKIDYTLAEEVKHSDDFLTYTVKLKKDLKWHDGKPLTADDLVFTMKQIMDEKQDSPFRSAFVINDKPVEVKKVDD
LTVEFKLPTVQMPFMNSLGQVSPIPKHVFEGEKDIKKSAKNEKPIGSGAFKFRESKKGESITLERFDNYVGGKPHLDTIT
YRIIADPNSSKVALENGEVSANYIDISGISKFEKNEKLKVVAYDEGMVDNLVLNCKAKGLDKKEVRQAIAYALNKDDLIK
AAYESEKYAPKAYSPLPKNALYYTEDVTKYDLNKDKAKELLKKSGAENLKLKLVYRNDKKTLENQALVVKENLKDIGIDV
ELKGLEANAFFKQIDDPSKADFDLIFNAYLMGNEPDAYKEVFMTNGAFNASRYNNKKLDDLWNKAAVETDKTKREEIYKT
IQKELIEDMPVYPICYSNATIAVNKNVGGIKEAKTAPIYMFQDLSKLYMIEK

Sequences:

>Translated_532_residues
MKVKKLLAMILVASTTLVATACGNSNSSSGSTSKETSAKENIKDGGNLVFSIRGEPEILNPIYAYDRDTMTMDNALFAPL
FYMNGDKIDYTLAEEVKHSDDFLTYTVKLKKDLKWHDGKPLTADDLVFTMKQIMDEKQDSPFRSAFVINDKPVEVKKVDD
LTVEFKLPTVQMPFMNSLGQVSPIPKHVFEGEKDIKKSAKNEKPIGSGAFKFRESKKGESITLERFDNYVGGKPHLDTIT
YRIIADPNSSKVALENGEVSANYIDISGISKFEKNEKLKVVAYDEGMVDNLVLNCKAKGLDKKEVRQAIAYALNKDDLIK
AAYESEKYAPKAYSPLPKNALYYTEDVTKYDLNKDKAKELLKKSGAENLKLKLVYRNDKKTLENQALVVKENLKDIGIDV
ELKGLEANAFFKQIDDPSKADFDLIFNAYLMGNEPDAYKEVFMTNGAFNASRYNNKKLDDLWNKAAVETDKTKREEIYKT
IQKELIEDMPVYPICYSNATIAVNKNVGGIKEAKTAPIYMFQDLSKLYMIEK
>Mature_532_residues
MKVKKLLAMILVASTTLVATACGNSNSSSGSTSKETSAKENIKDGGNLVFSIRGEPEILNPIYAYDRDTMTMDNALFAPL
FYMNGDKIDYTLAEEVKHSDDFLTYTVKLKKDLKWHDGKPLTADDLVFTMKQIMDEKQDSPFRSAFVINDKPVEVKKVDD
LTVEFKLPTVQMPFMNSLGQVSPIPKHVFEGEKDIKKSAKNEKPIGSGAFKFRESKKGESITLERFDNYVGGKPHLDTIT
YRIIADPNSSKVALENGEVSANYIDISGISKFEKNEKLKVVAYDEGMVDNLVLNCKAKGLDKKEVRQAIAYALNKDDLIK
AAYESEKYAPKAYSPLPKNALYYTEDVTKYDLNKDKAKELLKKSGAENLKLKLVYRNDKKTLENQALVVKENLKDIGIDV
ELKGLEANAFFKQIDDPSKADFDLIFNAYLMGNEPDAYKEVFMTNGAFNASRYNNKKLDDLWNKAAVETDKTKREEIYKT
IQKELIEDMPVYPICYSNATIAVNKNVGGIKEAKTAPIYMFQDLSKLYMIEK

Specific function: This protein is a component of an oligopeptide permease, a binding protein-dependent transport system. This APP system can completely substitute for the OPP system in both sporulation and genetic competence. AppA can bind and transport tetra- and pentapep

COG id: COG0747

COG function: function code E; ABC-type dipeptide transport system, periplasmic component

Gene ontology:

Cell location: Cell membrane; Lipid-anchor (Probable) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the bacterial solute-binding protein 5 family [H]

Homologues:

Organism=Escherichia coli, GI1789966, Length=501, Percent_Identity=27.7445109780439, Blast_Score=157, Evalue=2e-39,
Organism=Escherichia coli, GI1787762, Length=482, Percent_Identity=25.3112033195021, Blast_Score=132, Evalue=4e-32,
Organism=Escherichia coli, GI1789887, Length=485, Percent_Identity=23.9175257731959, Blast_Score=129, Evalue=5e-31,
Organism=Escherichia coli, GI1787052, Length=479, Percent_Identity=23.5908141962422, Blast_Score=124, Evalue=1e-29,
Organism=Escherichia coli, GI1787551, Length=518, Percent_Identity=23.3590733590734, Blast_Score=117, Evalue=1e-27,
Organism=Escherichia coli, GI1787495, Length=543, Percent_Identity=24.3093922651934, Blast_Score=101, Evalue=2e-22,
Organism=Escherichia coli, GI87081878, Length=526, Percent_Identity=24.3346007604563, Blast_Score=95, Evalue=1e-20,
Organism=Escherichia coli, GI1789397, Length=463, Percent_Identity=23.9740820734341, Blast_Score=92, Evalue=7e-20,
Organism=Escherichia coli, GI87082063, Length=470, Percent_Identity=21.4893617021277, Blast_Score=64, Evalue=2e-11,

Paralogues:

None

Copy number: 660 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 2980 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 40 Molecules/Cell In: Stationary Phase, Rich Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000914 [H]

Pfam domain/function: PF00496 SBP_bac_5 [H]

EC number: NA

Molecular weight: Translated: 59970; Mature: 59970

Theoretical pI: Translated: 6.96; Mature: 6.96

Prosite motif: PS00013 PROKAR_LIPOPROTEIN ; PS01040 SBP_BACTERIAL_5

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
2.8 %Met     (Translated Protein)
3.4 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
2.8 %Met     (Mature Protein)
3.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKVKKLLAMILVASTTLVATACGNSNSSSGSTSKETSAKENIKDGGNLVFSIRGEPEILN
CCHHHHHHHHHHHHHHEEEEECCCCCCCCCCCCCCCCHHHHCCCCCCEEEEECCCCCCCC
PIYAYDRDTMTMDNALFAPLFYMNGDKIDYTLAEEVKHSDDFLTYTVKLKKDLKWHDGKP
CHHHCCCCCEEECCHHEEEEEEECCCEEEEEHHHHHCCCCCEEEEEEEEEECCCCCCCCC
LTADDLVFTMKQIMDEKQDSPFRSAFVINDKPVEVKKVDDLTVEFKLPTVQMPFMNSLGQ
CCHHHHHHHHHHHHHHCCCCCCCEEEEECCCCCEEEEECCEEEEEECCEEECHHHHCCCC
VSPIPKHVFEGEKDIKKSAKNEKPIGSGAFKFRESKKGESITLERFDNYVGGKPHLDTIT
CCCCCHHHHCCHHHHHHHCCCCCCCCCCHHHHHHCCCCCEEEHHHHHCCCCCCCCCCEEE
YRIIADPNSSKVALENGEVSANYIDISGISKFEKNEKLKVVAYDEGMVDNLVLNCKAKGL
EEEEECCCCCEEEEECCCEEEEEEEECCCCHHCCCCCEEEEEECCCCCCCEEEEEECCCC
DKKEVRQAIAYALNKDDLIKAAYESEKYAPKAYSPLPKNALYYTEDVTKYDLNKDKAKEL
CHHHHHHHHHHHCCHHHHHHHHHHCCCCCCCCCCCCCCCCEEEECCCEEECCCHHHHHHH
LKKSGAENLKLKLVYRNDKKTLENQALVVKENLKDIGIDVELKGLEANAFFKQIDDPSKA
HHHCCCCCEEEEEEEECCCHHHHCCEEEEECCHHHCCCEEEEECCCHHHHHHHCCCCCCC
DFDLIFNAYLMGNEPDAYKEVFMTNGAFNASRYNNKKLDDLWNKAAVETDKTKREEIYKT
CCEEEEEEEEECCCCHHHHHHHEECCCCCCCCCCCCCHHHHHHHHHCCCCHHHHHHHHHH
IQKELIEDMPVYPICYSNATIAVNKNVGGIKEAKTAPIYMFQDLSKLYMIEK
HHHHHHHHCCCCEEEECCEEEEEECCCCCCCCCCCCCCHHHHHHHHHHCCCC
>Mature Secondary Structure
MKVKKLLAMILVASTTLVATACGNSNSSSGSTSKETSAKENIKDGGNLVFSIRGEPEILN
CCHHHHHHHHHHHHHHEEEEECCCCCCCCCCCCCCCCHHHHCCCCCCEEEEECCCCCCCC
PIYAYDRDTMTMDNALFAPLFYMNGDKIDYTLAEEVKHSDDFLTYTVKLKKDLKWHDGKP
CHHHCCCCCEEECCHHEEEEEEECCCEEEEEHHHHHCCCCCEEEEEEEEEECCCCCCCCC
LTADDLVFTMKQIMDEKQDSPFRSAFVINDKPVEVKKVDDLTVEFKLPTVQMPFMNSLGQ
CCHHHHHHHHHHHHHHCCCCCCCEEEEECCCCCEEEEECCEEEEEECCEEECHHHHCCCC
VSPIPKHVFEGEKDIKKSAKNEKPIGSGAFKFRESKKGESITLERFDNYVGGKPHLDTIT
CCCCCHHHHCCHHHHHHHCCCCCCCCCCHHHHHHCCCCCEEEHHHHHCCCCCCCCCCEEE
YRIIADPNSSKVALENGEVSANYIDISGISKFEKNEKLKVVAYDEGMVDNLVLNCKAKGL
EEEEECCCCCEEEEECCCEEEEEEEECCCCHHCCCCCEEEEEECCCCCCCEEEEEECCCC
DKKEVRQAIAYALNKDDLIKAAYESEKYAPKAYSPLPKNALYYTEDVTKYDLNKDKAKEL
CHHHHHHHHHHHCCHHHHHHHHHHCCCCCCCCCCCCCCCCEEEECCCEEECCCHHHHHHH
LKKSGAENLKLKLVYRNDKKTLENQALVVKENLKDIGIDVELKGLEANAFFKQIDDPSKA
HHHCCCCCEEEEEEEECCCHHHHCCEEEEECCHHHCCCEEEEECCCHHHHHHHCCCCCCC
DFDLIFNAYLMGNEPDAYKEVFMTNGAFNASRYNNKKLDDLWNKAAVETDKTKREEIYKT
CCEEEEEEEEECCCCHHHHHHHEECCCCCCCCCCCCCHHHHHHHHHCCCCHHHHHHHHHH
IQKELIEDMPVYPICYSNATIAVNKNVGGIKEAKTAPIYMFQDLSKLYMIEK
HHHHHHHHCCCCEEEECCEEEEEECCCCCCCCCCCCCCHHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: ATP; dipeptides [Periplasm]; H2O [C]

Specific reaction: ATP + dipeptides [Periplasm] + H2O = ADP + phosphate + dipeptides [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 7997159; 9384377 [H]