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The map label for this gene is ygaF [C]

Identifier: 226948346

GI number: 226948346

Start: 1279048

End: 1280466

Strand: Direct

Name: ygaF [C]

Synonym: CLM_1228

Alternate gene names: 226948346

Gene position: 1279048-1280466 (Clockwise)

Preceding gene: 226948345

Following gene: 226948347

Centisome position: 30.78

GC content: 32.42

Gene sequence:

>1419_bases
ATGTTTGATGTTACAATTATAGGTTCAGGGGTTACAGGAGCTGCTGTGGCTAGAGAATTATCTAAATATAATTTAAAAAC
TTGTGTAGTGGAAAAGGCAATAGATGTAGCTAATGGTACAAGTAAAGCTAATAGTGGTATTGTACATGCTGGTGAAGATC
CAATTCCAGGAACCCTAAAGGCTAAGATGAATGTTAGAGGAAATGAAATGTTCGACAAATTACAAGAAGAAATAGATTTC
CCATTTAAAAGAAATGAATCCTTTGTTTTATGCTTTGATGAAAAGGATATTGAAAAACTAGAAGAACTAAGACAAAGAGG
TTTAAAAAATGGTCTTCCAGATACAATGGAAATACTAAATAGAGAAGAAGCTTTAAAGTTAGAACCTAACTTATCAGAAT
ATGTAGTGGCTGCTCTTAGACTTCCTACAGGAGGGATAGTATCTCCTTATGAATTTAATATAGCTCTTGCAGAATCAGCA
GCTATGAACGGTGTAGAGTTTAAACTTGAAACAGAAATAATTGATATAGAGAAAAAAGAAGATGGATATATATTAAAAAC
TAATAAAGGTGATATAGAAACTAAAGTTGTAGTAAATGCAGCTGGAGTTTTTGGAGATAAAATAAACAACATGGTAAGTG
AAAAGAAATACCATATAACTGCAAGAAAAGGTGAATATTTATTATTTGACAAAACTGTAGGTGACATGGTTCAAAGAACA
ATATTCCAACTTCCAACGAAGATGGGTAAAGGGGTATTAGTTACTCCAACAGCAGATGGAAACTTGCTTTTAGGACCAAC
TTCTGTAGATGTAGAAGAAAAGGATGACCTTGGAACTACAAGAGAAGGACTAGATACTGTAGCAGAGAAAGCTAAATTAA
GTATAAAAGAAATACCAATGAGACAGGTTATAACATCCTTTGCAGGACTTAGAGCTCATGAAGAAAATAGTGATTTTATA
ATAGGAGAGGCAGAAGATGCTGAAAACTTCATAAATGCTATAGGAATAGAATCACCTGGTTTAACTAGTGCACCAGCTAT
AGGTGAGTATATAAGAGAGATGATAGTTGAAAAATTAAAACCAGAAGAAAATAAAGAGTTTAATCCAATTAGAAAAGATA
TACCTAAATTTAGAGAAATGACAAATGAAGAAAGAAAAGAAATGATAAAAGAAAATTCTGCTTACGGAAAAATAGTCTGT
AGATGTGAAGTAGTAACTGAAGGAGAAATAAGAGATGCTATAAGAAGACCTTTAGGTGCTAAAACTGTAGACGGAATAAA
AAGAAGAACAAGAGCAGGTATGGGAAGATGCCAATCAGGGTTCTGTTCAAACAGAATAGTTGAAATATTAGCAGAAGAGT
TAGGTATAAAAAGAAATGAAGTTACTAAGTTTGGCGGAAATTCAAAAATATTATATTAG

Upstream 100 bases:

>100_bases
ATTATTTCAGGCAGAGATATTGAGAGCCTTAATTTAAAACGGGATTATTATACCCATTTTAAATTAAGGCTCTTTTGATT
TATAAGGAGGAGTAAGTAAT

Downstream 100 bases:

>100_bases
AAAACTAATTTAAATTAATGAGGTAATTTAAAGCAAATGCATTGGAGGGCGTAGTATGCAACAATATGATGTAGTTATAA
TTGGTGGAGGCCCAGCAGGT

Product: FAD-dependent oxidoreductase

Products: glycerone phosphate; reduced acceptor

Alternate protein names: NA

Number of amino acids: Translated: 472; Mature: 472

Protein sequence:

>472_residues
MFDVTIIGSGVTGAAVARELSKYNLKTCVVEKAIDVANGTSKANSGIVHAGEDPIPGTLKAKMNVRGNEMFDKLQEEIDF
PFKRNESFVLCFDEKDIEKLEELRQRGLKNGLPDTMEILNREEALKLEPNLSEYVVAALRLPTGGIVSPYEFNIALAESA
AMNGVEFKLETEIIDIEKKEDGYILKTNKGDIETKVVVNAAGVFGDKINNMVSEKKYHITARKGEYLLFDKTVGDMVQRT
IFQLPTKMGKGVLVTPTADGNLLLGPTSVDVEEKDDLGTTREGLDTVAEKAKLSIKEIPMRQVITSFAGLRAHEENSDFI
IGEAEDAENFINAIGIESPGLTSAPAIGEYIREMIVEKLKPEENKEFNPIRKDIPKFREMTNEERKEMIKENSAYGKIVC
RCEVVTEGEIRDAIRRPLGAKTVDGIKRRTRAGMGRCQSGFCSNRIVEILAEELGIKRNEVTKFGGNSKILY

Sequences:

>Translated_472_residues
MFDVTIIGSGVTGAAVARELSKYNLKTCVVEKAIDVANGTSKANSGIVHAGEDPIPGTLKAKMNVRGNEMFDKLQEEIDF
PFKRNESFVLCFDEKDIEKLEELRQRGLKNGLPDTMEILNREEALKLEPNLSEYVVAALRLPTGGIVSPYEFNIALAESA
AMNGVEFKLETEIIDIEKKEDGYILKTNKGDIETKVVVNAAGVFGDKINNMVSEKKYHITARKGEYLLFDKTVGDMVQRT
IFQLPTKMGKGVLVTPTADGNLLLGPTSVDVEEKDDLGTTREGLDTVAEKAKLSIKEIPMRQVITSFAGLRAHEENSDFI
IGEAEDAENFINAIGIESPGLTSAPAIGEYIREMIVEKLKPEENKEFNPIRKDIPKFREMTNEERKEMIKENSAYGKIVC
RCEVVTEGEIRDAIRRPLGAKTVDGIKRRTRAGMGRCQSGFCSNRIVEILAEELGIKRNEVTKFGGNSKILY
>Mature_472_residues
MFDVTIIGSGVTGAAVARELSKYNLKTCVVEKAIDVANGTSKANSGIVHAGEDPIPGTLKAKMNVRGNEMFDKLQEEIDF
PFKRNESFVLCFDEKDIEKLEELRQRGLKNGLPDTMEILNREEALKLEPNLSEYVVAALRLPTGGIVSPYEFNIALAESA
AMNGVEFKLETEIIDIEKKEDGYILKTNKGDIETKVVVNAAGVFGDKINNMVSEKKYHITARKGEYLLFDKTVGDMVQRT
IFQLPTKMGKGVLVTPTADGNLLLGPTSVDVEEKDDLGTTREGLDTVAEKAKLSIKEIPMRQVITSFAGLRAHEENSDFI
IGEAEDAENFINAIGIESPGLTSAPAIGEYIREMIVEKLKPEENKEFNPIRKDIPKFREMTNEERKEMIKENSAYGKIVC
RCEVVTEGEIRDAIRRPLGAKTVDGIKRRTRAGMGRCQSGFCSNRIVEILAEELGIKRNEVTKFGGNSKILY

Specific function: Unknown

COG id: COG0579

COG function: function code R; Predicted dehydrogenase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: NA

Homologues:

Organism=Homo sapiens, GI13376331, Length=271, Percent_Identity=26.5682656826568, Blast_Score=96, Evalue=5e-20,
Organism=Homo sapiens, GI194306651, Length=377, Percent_Identity=23.8726790450928, Blast_Score=68, Evalue=2e-11,
Organism=Escherichia coli, GI87082148, Length=248, Percent_Identity=24.5967741935484, Blast_Score=87, Evalue=2e-18,
Organism=Escherichia coli, GI1788574, Length=475, Percent_Identity=20.4210526315789, Blast_Score=74, Evalue=1e-14,
Organism=Caenorhabditis elegans, GI17565764, Length=292, Percent_Identity=28.4246575342466, Blast_Score=107, Evalue=2e-23,
Organism=Drosophila melanogaster, GI24585081, Length=293, Percent_Identity=30.0341296928328, Blast_Score=100, Evalue=2e-21,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR006076 [H]

Pfam domain/function: PF01266 DAO [H]

EC number: 1.1.99.5

Molecular weight: Translated: 52337; Mature: 52337

Theoretical pI: Translated: 4.99; Mature: 4.99

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.3 %Cys     (Translated Protein)
2.8 %Met     (Translated Protein)
4.0 %Cys+Met (Translated Protein)
1.3 %Cys     (Mature Protein)
2.8 %Met     (Mature Protein)
4.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MFDVTIIGSGVTGAAVARELSKYNLKTCVVEKAIDVANGTSKANSGIVHAGEDPIPGTLK
CEEEEEECCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCEEECCCCCCCCCEE
AKMNVRGNEMFDKLQEEIDFPFKRNESFVLCFDEKDIEKLEELRQRGLKNGLPDTMEILN
EEECCCCHHHHHHHHHHHCCCCCCCCCEEEEECCHHHHHHHHHHHHHHCCCCCHHHHHHC
REEALKLEPNLSEYVVAALRLPTGGIVSPYEFNIALAESAAMNGVEFKLETEIIDIEKKE
CHHCEEECCCHHHHHHHHHCCCCCCCCCCEEEEEEEECHHCCCCEEEEEEEEEEEEEECC
DGYILKTNKGDIETKVVVNAAGVFGDKINNMVSEKKYHITARKGEYLLFDKTVGDMVQRT
CCEEEECCCCCCEEEEEEEECHHHHHHHHHHHHCCEEEEEECCCCEEEEECHHHHHHHHH
IFQLPTKMGKGVLVTPTADGNLLLGPTSVDVEEKDDLGTTREGLDTVAEKAKLSIKEIPM
HHHHHHHHCCCEEEEECCCCCEEECCCCCCCCCCCCCCCCHHHHHHHHHHHHCHHHHCCH
RQVITSFAGLRAHEENSDFIIGEAEDAENFINAIGIESPGLTSAPAIGEYIREMIVEKLK
HHHHHHHHHCCCCCCCCCEEEECCHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHHCC
PEENKEFNPIRKDIPKFREMTNEERKEMIKENSAYGKIVCRCEVVTEGEIRDAIRRPLGA
CCCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEEEEEEEECCHHHHHHHCCCCC
KTVDGIKRRTRAGMGRCQSGFCSNRIVEILAEELGIKRNEVTKFGGNSKILY
HHHHHHHHHHHHHHCHHCCCCHHHHHHHHHHHHHCCCHHHHHCCCCCCCCCC
>Mature Secondary Structure
MFDVTIIGSGVTGAAVARELSKYNLKTCVVEKAIDVANGTSKANSGIVHAGEDPIPGTLK
CEEEEEECCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCEEECCCCCCCCCEE
AKMNVRGNEMFDKLQEEIDFPFKRNESFVLCFDEKDIEKLEELRQRGLKNGLPDTMEILN
EEECCCCHHHHHHHHHHHCCCCCCCCCEEEEECCHHHHHHHHHHHHHHCCCCCHHHHHHC
REEALKLEPNLSEYVVAALRLPTGGIVSPYEFNIALAESAAMNGVEFKLETEIIDIEKKE
CHHCEEECCCHHHHHHHHHCCCCCCCCCCEEEEEEEECHHCCCCEEEEEEEEEEEEEECC
DGYILKTNKGDIETKVVVNAAGVFGDKINNMVSEKKYHITARKGEYLLFDKTVGDMVQRT
CCEEEECCCCCCEEEEEEEECHHHHHHHHHHHHCCEEEEEECCCCEEEEECHHHHHHHHH
IFQLPTKMGKGVLVTPTADGNLLLGPTSVDVEEKDDLGTTREGLDTVAEKAKLSIKEIPM
HHHHHHHHCCCEEEEECCCCCEEECCCCCCCCCCCCCCCCHHHHHHHHHHHHCHHHHCCH
RQVITSFAGLRAHEENSDFIIGEAEDAENFINAIGIESPGLTSAPAIGEYIREMIVEKLK
HHHHHHHHHCCCCCCCCCEEEECCHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHHCC
PEENKEFNPIRKDIPKFREMTNEERKEMIKENSAYGKIVCRCEVVTEGEIRDAIRRPLGA
CCCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEEEEEEEECCHHHHHHHCCCCC
KTVDGIKRRTRAGMGRCQSGFCSNRIVEILAEELGIKRNEVTKFGGNSKILY
HHHHHHHHHHHHHHCHHCCCCHHHHHHHHHHHHHCCCHHHHHCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: sn-glycerol 3-phosphate; acceptor

Specific reaction: sn-glycerol 3-phosphate + acceptor = glycerone phosphate + reduced acceptor

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8948633 [H]