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The map label for this gene is cobS

Identifier: 226948099

GI number: 226948099

Start: 1025541

End: 1026287

Strand: Direct

Name: cobS

Synonym: CLM_0963

Alternate gene names: 226948099

Gene position: 1025541-1026287 (Clockwise)

Preceding gene: 226948098

Following gene: 226948100

Centisome position: 24.68

GC content: 25.97

Gene sequence:

>747_bases
ATGAAGAGTATATTAAATGATTTTTTATTAATGATTCAATTTTTTACTCGCATACCTATAAATAAAAACTTACAATGTGA
AAAAGTAAATTTTAGAAGGGGAGCTTTTTTTCTTCCAGTAGTTGCTTTCATTATAGGTGGAATGGAGTTTTTAATATATT
TAGGTCTTAAAAATTTTTTACCCGCTAATGTAATAATAGTGTTATTAATTTTGTTTACAGCTATGGTTACTGGAGGCCTT
CATATGGATGGATTAGCAGATACCTGTGATGGATTTTTTTCTTTAAGAGATAAGGAAAGAATAATAGAAATTATGAAAGA
CAGTAGAATAGGGTCCTACGGAACCATAGCATTAATAATTGATTTACTATTAAAATATCAACTTCTATATTCTTTAGTTT
TAAAAGGATACAGTATAGCTATAGTCTTAGCGCCCATCATAGGAAGAATTAGTATTTTATTTTTATGTTTATCTAAAAGA
ACAGCGAAAAAAAATGGCTCAGGAAATATATTTATAGGAAATATGAGCAAACCTATAGTATTTTTTATTACCACAATAAT
ATTAGCGTTAAGTACATATTTTTTAGGATTAAGAGCTACAATAATTCCATTTATAGGAGCTTTGTTAATAACTTATTTAT
TATATTTATTATGTTTAAATAAAATAAATGGACTTACAGGTGATACCTTGGGAGCTTGTAATGAACTAGGTGAAATAACA
TTTTTATTAATTTTATTAATGATGTAA

Upstream 100 bases:

>100_bases
CTTTGAAGAAGATGGGAGTATTAGAGCGGGTAGTCATCGGATAAATTTTTGTTTGTACGAAATTTAGGTAATTAATAATA
AATATAAAAGAGGTGGAAAC

Downstream 100 bases:

>100_bases
AAACTTTAATAGATGGTGTTTCAAAATAAAATAGATTTAATTTTAAAGGCGCAAAGATAAAAAATACACTCATAAACCAC
TTCATTTCGCTAAGAAGTTC

Product: cobalamin synthase

Products: GMP; adenosylcobalamin

Alternate protein names: NA

Number of amino acids: Translated: 248; Mature: 248

Protein sequence:

>248_residues
MKSILNDFLLMIQFFTRIPINKNLQCEKVNFRRGAFFLPVVAFIIGGMEFLIYLGLKNFLPANVIIVLLILFTAMVTGGL
HMDGLADTCDGFFSLRDKERIIEIMKDSRIGSYGTIALIIDLLLKYQLLYSLVLKGYSIAIVLAPIIGRISILFLCLSKR
TAKKNGSGNIFIGNMSKPIVFFITTIILALSTYFLGLRATIIPFIGALLITYLLYLLCLNKINGLTGDTLGACNELGEIT
FLLILLMM

Sequences:

>Translated_248_residues
MKSILNDFLLMIQFFTRIPINKNLQCEKVNFRRGAFFLPVVAFIIGGMEFLIYLGLKNFLPANVIIVLLILFTAMVTGGL
HMDGLADTCDGFFSLRDKERIIEIMKDSRIGSYGTIALIIDLLLKYQLLYSLVLKGYSIAIVLAPIIGRISILFLCLSKR
TAKKNGSGNIFIGNMSKPIVFFITTIILALSTYFLGLRATIIPFIGALLITYLLYLLCLNKINGLTGDTLGACNELGEIT
FLLILLMM
>Mature_248_residues
MKSILNDFLLMIQFFTRIPINKNLQCEKVNFRRGAFFLPVVAFIIGGMEFLIYLGLKNFLPANVIIVLLILFTAMVTGGL
HMDGLADTCDGFFSLRDKERIIEIMKDSRIGSYGTIALIIDLLLKYQLLYSLVLKGYSIAIVLAPIIGRISILFLCLSKR
TAKKNGSGNIFIGNMSKPIVFFITTIILALSTYFLGLRATIIPFIGALLITYLLYLLCLNKINGLTGDTLGACNELGEIT
FLLILLMM

Specific function: Joins Ado-cobinamide-GDP and alpha-ribazole to generate adenosylcobalamin (Ado-cobalamin)

COG id: COG0368

COG function: function code H; Cobalamin-5-phosphate synthase

Gene ontology:

Cell location: Cell membrane; Multi-pass membrane protein

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the CobS family

Homologues:

Organism=Escherichia coli, GI1788301, Length=247, Percent_Identity=34.8178137651822, Blast_Score=144, Evalue=6e-36,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): COBS_CLOBJ (C1FUP2)

Other databases:

- EMBL:   CP001581
- RefSeq:   YP_002803190.1
- GeneID:   7764501
- GenomeReviews:   CP001581_GR
- KEGG:   cby:CLM_0963
- OMA:   IMKDPTS
- ProtClustDB:   PRK00235
- HAMAP:   MF_00719
- InterPro:   IPR003805
- TIGRFAMs:   TIGR00317

Pfam domain/function: PF02654 CobS

EC number: 2.7.8.26

Molecular weight: Translated: 27611; Mature: 27611

Theoretical pI: Translated: 9.56; Mature: 9.56

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

HASH(0x403f4a6c)-; HASH(0x132cd320)-; HASH(0x125b9910)-; HASH(0x138e834c)-; HASH(0x1129e59c)-; HASH(0x13728c24)-;

Cys/Met content:

2.0 %Cys     (Translated Protein)
3.6 %Met     (Translated Protein)
5.6 %Cys+Met (Translated Protein)
2.0 %Cys     (Mature Protein)
3.6 %Met     (Mature Protein)
5.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKSILNDFLLMIQFFTRIPINKNLQCEKVNFRRGAFFLPVVAFIIGGMEFLIYLGLKNFL
CHHHHHHHHHHHHHHHHCCCCCCCCEEECCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCC
PANVIIVLLILFTAMVTGGLHMDGLADTCDGFFSLRDKERIIEIMKDSRIGSYGTIALII
HHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHH
DLLLKYQLLYSLVLKGYSIAIVLAPIIGRISILFLCLSKRTAKKNGSGNIFIGNMSKPIV
HHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCEEECCCCCHHH
FFITTIILALSTYFLGLRATIIPFIGALLITYLLYLLCLNKINGLTGDTLGACNELGEIT
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHH
FLLILLMM
HHHHHHHH
>Mature Secondary Structure
MKSILNDFLLMIQFFTRIPINKNLQCEKVNFRRGAFFLPVVAFIIGGMEFLIYLGLKNFL
CHHHHHHHHHHHHHHHHCCCCCCCCEEECCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCC
PANVIIVLLILFTAMVTGGLHMDGLADTCDGFFSLRDKERIIEIMKDSRIGSYGTIALII
HHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHH
DLLLKYQLLYSLVLKGYSIAIVLAPIIGRISILFLCLSKRTAKKNGSGNIFIGNMSKPIV
HHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCEEECCCCCHHH
FFITTIILALSTYFLGLRATIIPFIGALLITYLLYLLCLNKINGLTGDTLGACNELGEIT
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHH
FLLILLMM
HHHHHHHH

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: adenosylcobinamide-GDP; alpha-ribazole

Specific reaction: adenosylcobinamide-GDP + alpha-ribazole = GMP + adenosylcobalamin

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: NA