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The map label for this gene is gcvH [H]

Identifier: 226947957

GI number: 226947957

Start: 878728

End: 879120

Strand: Direct

Name: gcvH [H]

Synonym: CLM_0816

Alternate gene names: 226947957

Gene position: 878728-879120 (Clockwise)

Preceding gene: 226947956

Following gene: 226947958

Centisome position: 21.15

GC content: 32.82

Gene sequence:

>393_bases
ATGAAAGTTTTAAATAATTTACTTTACACTGGTGATCATGAATGGATAAGGGTAGAAGACAACAAAGCATATATAGGAAT
CAGCGATTGTGCACAACGTATGTTAAGCGATATAGTTTTTGTTGAATTACCAGAGGTTGATGATGAAATAGCAAAAGGTG
AAACTTTTGCAACTATAGAATCAGTTAAAGCAGCATCAGACAGTTACATGCCTGTTAGTGGAACAATTGTAGAAATTAAT
GAAGAACTAGAAGATAATCCAGCAGCATTAAACGAAGACCCATATGGAAGTTGGATTATTGCAGTAGAGATGTCAGATAA
AAGTGAATTAGAAGAATTAATAAAACCAGAAGTTTATGAGAAAATATGTGAAGAGTTAGATAAGGAGGCATAA

Upstream 100 bases:

>100_bases
TATTTTTTAATATATATAAATTCACACTAATGTTTAGTTTTCAGGTTAAAATGTCATTGGTAATCAATATATAATAATTT
AAAAATTGGGGGTTAGTAGA

Downstream 100 bases:

>100_bases
TTATGTTTCCATATATTCCAAACACAGAAAAAGAAACAAAAGAAATTTTAGATTTTCTTTCAATAAAATCTGTAGATGAA
TTATTTAGTGATATACCAGA

Product: glycine cleavage system protein H

Products: Proton; NADH; NH3; CO2; 5,10-methylene-THF [C]

Alternate protein names: NA

Number of amino acids: Translated: 130; Mature: 130

Protein sequence:

>130_residues
MKVLNNLLYTGDHEWIRVEDNKAYIGISDCAQRMLSDIVFVELPEVDDEIAKGETFATIESVKAASDSYMPVSGTIVEIN
EELEDNPAALNEDPYGSWIIAVEMSDKSELEELIKPEVYEKICEELDKEA

Sequences:

>Translated_130_residues
MKVLNNLLYTGDHEWIRVEDNKAYIGISDCAQRMLSDIVFVELPEVDDEIAKGETFATIESVKAASDSYMPVSGTIVEIN
EELEDNPAALNEDPYGSWIIAVEMSDKSELEELIKPEVYEKICEELDKEA
>Mature_130_residues
MKVLNNLLYTGDHEWIRVEDNKAYIGISDCAQRMLSDIVFVELPEVDDEIAKGETFATIESVKAASDSYMPVSGTIVEIN
EELEDNPAALNEDPYGSWIIAVEMSDKSELEELIKPEVYEKICEELDKEA

Specific function: The glycine cleavage system catalyzes the degradation of glycine. The H protein shuttles the methylamine group of glycine from the P protein to the T protein [H]

COG id: COG0509

COG function: function code E; Glycine cleavage system H protein (lipoate-binding)

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 lipoyl-binding domain [H]

Homologues:

Organism=Homo sapiens, GI49574537, Length=114, Percent_Identity=44.7368421052632, Blast_Score=104, Evalue=2e-23,
Organism=Homo sapiens, GI89057342, Length=114, Percent_Identity=43.859649122807, Blast_Score=101, Evalue=2e-22,
Organism=Escherichia coli, GI1789271, Length=121, Percent_Identity=47.9338842975207, Blast_Score=114, Evalue=2e-27,
Organism=Caenorhabditis elegans, GI17551294, Length=126, Percent_Identity=45.2380952380952, Blast_Score=110, Evalue=3e-25,
Organism=Caenorhabditis elegans, GI17507493, Length=116, Percent_Identity=42.2413793103448, Blast_Score=103, Evalue=2e-23,
Organism=Saccharomyces cerevisiae, GI6319272, Length=120, Percent_Identity=45, Blast_Score=106, Evalue=1e-24,
Organism=Drosophila melanogaster, GI17865652, Length=114, Percent_Identity=42.1052631578947, Blast_Score=92, Evalue=7e-20,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003016
- InterPro:   IPR002930
- InterPro:   IPR017453
- InterPro:   IPR011053 [H]

Pfam domain/function: PF01597 GCV_H [H]

EC number: NA

Molecular weight: Translated: 14647; Mature: 14647

Theoretical pI: Translated: 3.77; Mature: 3.77

Prosite motif: PS00189 LIPOYL

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.5 %Cys     (Translated Protein)
3.1 %Met     (Translated Protein)
4.6 %Cys+Met (Translated Protein)
1.5 %Cys     (Mature Protein)
3.1 %Met     (Mature Protein)
4.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKVLNNLLYTGDHEWIRVEDNKAYIGISDCAQRMLSDIVFVELPEVDDEIAKGETFATIE
CCHHHCCEECCCCCEEEEECCCEEEEHHHHHHHHHHHEEEEECCCCCCHHHCCCHHHHHH
SVKAASDSYMPVSGTIVEINEELEDNPAALNEDPYGSWIIAVEMSDKSELEELIKPEVYE
HHHHCCCCCCCCCCEEEEECCCCCCCCCCCCCCCCCCEEEEEEECCHHHHHHHHCHHHHH
KICEELDKEA
HHHHHHCCCC
>Mature Secondary Structure
MKVLNNLLYTGDHEWIRVEDNKAYIGISDCAQRMLSDIVFVELPEVDDEIAKGETFATIE
CCHHHCCEECCCCCEEEEECCCEEEEHHHHHHHHHHHEEEEECCCCCCHHHCCCHHHHHH
SVKAASDSYMPVSGTIVEINEELEDNPAALNEDPYGSWIIAVEMSDKSELEELIKPEVYE
HHHHCCCCCCCCCCEEEEECCCCCCCCCCCCCCCCCCEEEEEEECCHHHHHHHHCHHHHH
KICEELDKEA
HHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: Lipoyl Cofactor. [C]

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NAD; L-glycine; THF [C]

Specific reaction: NAD + L-glycine + THF = Proton + NADH + NH3 + CO2 + 5,10-methylene-THF [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA