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The map label for this gene is vanS [H]

Identifier: 226947864

GI number: 226947864

Start: 772597

End: 773679

Strand: Direct

Name: vanS [H]

Synonym: CLM_0719

Alternate gene names: 226947864

Gene position: 772597-773679 (Clockwise)

Preceding gene: 226947863

Following gene: 226947868

Centisome position: 18.59

GC content: 26.22

Gene sequence:

>1083_bases
TTGGGAGATATATTAATAGCTTTAATATTAACTTACACAACAGCATATACAAGTAAGAGAATATTAAACTATTTATATGT
AAAGTTTGACAATGAGTTTGCTTTTTATTTTTGGATGTTTTGGAGAAATGTTAAATTTGCAACAGTTGGTCAATCTCTTA
ATATAATAGTTGGAGTAGTTTTATTTTCAATGCTTTATTTGATGATAACTTATAGAAAAGCTAAAAATTTTGTAGCTATT
ATAGAGGAAACTGAAATAATGGCTAATGGGGACTTAGATAGAGTAATACGGGTTAATGCAGAAGGGGATATTAAAAATTT
AGCAGAAAATATAAATAATATATCAAAGCAACTTAAAGATATAACAGTAGCAGAAAGAAACGCACAGAAAACCAAAAATG
ATTTGATAACTAATGTCTCCCACGATTTAAGAACTCCATTGACTTCAATAATGGGCTATTTAGAACTTATTGATAATGAT
CAATATAAAGATGAAGTGGCACTTAGATATTATGTTAATATAGCCTATGAAAAATCTAAGGGGTTAAACTTGCTTATAAA
GGATTTGTTTGAACTTACTAAAATGCAAAATAATACTATTAATCTAGAGAAAGTTGATATTAATTTAGTGGAATTATTAG
GGCAAGTAGTTGCTTGTTTTGAATATCAATTTAAAAGTGCAAATATGGAATCAAGAATTAATTTTTCAAACGATAAGCTA
ATAGTAAATGCAGATGCAGGAAAACTAGTAAGAGCATTTGAAAATTTATTATCTAATGCAATTAAATATGGGAAAGACGG
TTATTATGTTGATGTAGTAACAAAACTTGAAGAAAATATGGCAGTAATTCAAATTATAAATTATGGTCAATTAATACCAA
CAGTAGACTTACCATATATATTTGATAGATTCTATAGAGTAGAAAAATCAAGAAATAACAAAATAGGCGGATCAGGATTA
GGTTTAGCTATAACGAAAAATATTATAGAATTGCATGAAGGTACTATAAAAGCATATAGTAATGACAATAAAACTGTATT
TGAAATAAAATTACCTATTAAGATTAATATAAATAGCAATTAA

Upstream 100 bases:

>100_bases
ATAGAACAACATATGGAAGGTAGTAAGGTAATCCATACTGTGTGGGGAGTTGGTTATAAAATTGAAAAATAAAAGATTAC
ATAAATATTGTTTATCGTCG

Downstream 100 bases:

>100_bases
ATAAAAATAATAATATATGTTTATATGATACCAATAGATTAATAAATTATAAAGCCCCATGCAAATGCATAGGGTTTTTG
AATAATCTAAGCAAATATAA

Product: sensor histidine kinase

Products: NA

Alternate protein names: Vancomycin histidine protein kinase; Vancomycin resistance protein vanS [H]

Number of amino acids: Translated: 360; Mature: 359

Protein sequence:

>360_residues
MGDILIALILTYTTAYTSKRILNYLYVKFDNEFAFYFWMFWRNVKFATVGQSLNIIVGVVLFSMLYLMITYRKAKNFVAI
IEETEIMANGDLDRVIRVNAEGDIKNLAENINNISKQLKDITVAERNAQKTKNDLITNVSHDLRTPLTSIMGYLELIDND
QYKDEVALRYYVNIAYEKSKGLNLLIKDLFELTKMQNNTINLEKVDINLVELLGQVVACFEYQFKSANMESRINFSNDKL
IVNADAGKLVRAFENLLSNAIKYGKDGYYVDVVTKLEENMAVIQIINYGQLIPTVDLPYIFDRFYRVEKSRNNKIGGSGL
GLAITKNIIELHEGTIKAYSNDNKTVFEIKLPIKININSN

Sequences:

>Translated_360_residues
MGDILIALILTYTTAYTSKRILNYLYVKFDNEFAFYFWMFWRNVKFATVGQSLNIIVGVVLFSMLYLMITYRKAKNFVAI
IEETEIMANGDLDRVIRVNAEGDIKNLAENINNISKQLKDITVAERNAQKTKNDLITNVSHDLRTPLTSIMGYLELIDND
QYKDEVALRYYVNIAYEKSKGLNLLIKDLFELTKMQNNTINLEKVDINLVELLGQVVACFEYQFKSANMESRINFSNDKL
IVNADAGKLVRAFENLLSNAIKYGKDGYYVDVVTKLEENMAVIQIINYGQLIPTVDLPYIFDRFYRVEKSRNNKIGGSGL
GLAITKNIIELHEGTIKAYSNDNKTVFEIKLPIKININSN
>Mature_359_residues
GDILIALILTYTTAYTSKRILNYLYVKFDNEFAFYFWMFWRNVKFATVGQSLNIIVGVVLFSMLYLMITYRKAKNFVAII
EETEIMANGDLDRVIRVNAEGDIKNLAENINNISKQLKDITVAERNAQKTKNDLITNVSHDLRTPLTSIMGYLELIDNDQ
YKDEVALRYYVNIAYEKSKGLNLLIKDLFELTKMQNNTINLEKVDINLVELLGQVVACFEYQFKSANMESRINFSNDKLI
VNADAGKLVRAFENLLSNAIKYGKDGYYVDVVTKLEENMAVIQIINYGQLIPTVDLPYIFDRFYRVEKSRNNKIGGSGLG
LAITKNIIELHEGTIKAYSNDNKTVFEIKLPIKININSN

Specific function: Member of the two-component regulatory system vanS/vanR. Activates the transcription of vanH, vanA and vanX in response to vancomycin which results in vancomycin resistance. VanS activates vanR by phosphorylation [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cell membrane; Multi-pass membrane protein (Potential) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 histidine kinase domain [H]

Homologues:

Organism=Escherichia coli, GI1788393, Length=283, Percent_Identity=27.9151943462898, Blast_Score=124, Evalue=1e-29,
Organism=Escherichia coli, GI1786783, Length=297, Percent_Identity=28.2828282828283, Blast_Score=123, Evalue=2e-29,
Organism=Escherichia coli, GI1786600, Length=240, Percent_Identity=30.4166666666667, Blast_Score=104, Evalue=1e-23,
Organism=Escherichia coli, GI1786912, Length=252, Percent_Identity=26.1904761904762, Blast_Score=93, Evalue=2e-20,
Organism=Escherichia coli, GI1789149, Length=265, Percent_Identity=27.9245283018868, Blast_Score=87, Evalue=2e-18,
Organism=Escherichia coli, GI145693157, Length=242, Percent_Identity=25.2066115702479, Blast_Score=85, Evalue=8e-18,
Organism=Escherichia coli, GI1788713, Length=238, Percent_Identity=26.890756302521, Blast_Score=85, Evalue=9e-18,
Organism=Escherichia coli, GI87082128, Length=232, Percent_Identity=26.7241379310345, Blast_Score=84, Evalue=2e-17,
Organism=Escherichia coli, GI1790346, Length=221, Percent_Identity=26.6968325791855, Blast_Score=78, Evalue=8e-16,
Organism=Escherichia coli, GI1790436, Length=228, Percent_Identity=30.2631578947368, Blast_Score=75, Evalue=9e-15,
Organism=Escherichia coli, GI1789808, Length=254, Percent_Identity=25.1968503937008, Blast_Score=74, Evalue=1e-14,
Organism=Escherichia coli, GI48994928, Length=237, Percent_Identity=25.3164556962025, Blast_Score=74, Evalue=2e-14,
Organism=Escherichia coli, GI1790861, Length=199, Percent_Identity=25.1256281407035, Blast_Score=70, Evalue=2e-13,
Organism=Escherichia coli, GI1788549, Length=237, Percent_Identity=27.8481012658228, Blast_Score=70, Evalue=3e-13,
Organism=Escherichia coli, GI1788279, Length=311, Percent_Identity=27.6527331189711, Blast_Score=70, Evalue=3e-13,
Organism=Escherichia coli, GI1787894, Length=230, Percent_Identity=27.3913043478261, Blast_Score=69, Evalue=5e-13,
Organism=Escherichia coli, GI87081816, Length=262, Percent_Identity=20.9923664122137, Blast_Score=68, Evalue=1e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003594
- InterPro:   IPR004358
- InterPro:   IPR003661
- InterPro:   IPR005467
- InterPro:   IPR009082 [H]

Pfam domain/function: PF02518 HATPase_c; PF00512 HisKA [H]

EC number: =2.7.13.3 [H]

Molecular weight: Translated: 41272; Mature: 41141

Theoretical pI: Translated: 7.46; Mature: 7.46

Prosite motif: PS50109 HIS_KIN

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
2.5 %Met     (Translated Protein)
2.8 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
2.2 %Met     (Mature Protein)
2.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MGDILIALILTYTTAYTSKRILNYLYVKFDNEFAFYFWMFWRNVKFATVGQSLNIIVGVV
CCHHHHHHHHHHHHHHHHHHHHEEEEEEECCCEEEEEEEHHHCCEEEECCCHHHHHHHHH
LFSMLYLMITYRKAKNFVAIIEETEIMANGDLDRVIRVNAEGDIKNLAENINNISKQLKD
HHHHHHHHHHHHHCCCEEEEEECCEEEECCCCCCEEEECCCCCHHHHHHHHHHHHHHHHH
ITVAERNAQKTKNDLITNVSHDLRTPLTSIMGYLELIDNDQYKDEVALRYYVNIAYEKSK
HHHHHCCHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHCCCCCHHHEEEEEEEEEEEECCC
GLNLLIKDLFELTKMQNNTINLEKVDINLVELLGQVVACFEYQFKSANMESRINFSNDKL
CCHHHHHHHHHHHHHCCCCEEEEEECHHHHHHHHHHHHHHHHHHCCCCCCCCEEECCCEE
IVNADAGKLVRAFENLLSNAIKYGKDGYYVDVVTKLEENMAVIQIINYGQLIPTVDLPYI
EEECCCHHHHHHHHHHHHHHHHHCCCCEEEEEEEHHHCCEEEEEEECCCCCCCCCCCHHH
FDRFYRVEKSRNNKIGGSGLGLAITKNIIELHEGTIKAYSNDNKTVFEIKLPIKININSN
HHHHHHHHHCCCCCCCCCCCCHHHHHHHHHHCCCEEEEECCCCCEEEEEEEEEEEEECCC
>Mature Secondary Structure 
GDILIALILTYTTAYTSKRILNYLYVKFDNEFAFYFWMFWRNVKFATVGQSLNIIVGVV
CHHHHHHHHHHHHHHHHHHHHEEEEEEECCCEEEEEEEHHHCCEEEECCCHHHHHHHHH
LFSMLYLMITYRKAKNFVAIIEETEIMANGDLDRVIRVNAEGDIKNLAENINNISKQLKD
HHHHHHHHHHHHHCCCEEEEEECCEEEECCCCCCEEEECCCCCHHHHHHHHHHHHHHHHH
ITVAERNAQKTKNDLITNVSHDLRTPLTSIMGYLELIDNDQYKDEVALRYYVNIAYEKSK
HHHHHCCHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHCCCCCHHHEEEEEEEEEEEECCC
GLNLLIKDLFELTKMQNNTINLEKVDINLVELLGQVVACFEYQFKSANMESRINFSNDKL
CCHHHHHHHHHHHHHCCCCEEEEEECHHHHHHHHHHHHHHHHHHCCCCCCCCEEECCCEE
IVNADAGKLVRAFENLLSNAIKYGKDGYYVDVVTKLEENMAVIQIINYGQLIPTVDLPYI
EEECCCHHHHHHHHHHHHHHHHHCCCCEEEEEEEHHHCCEEEEEEECCCCCCCCCCCHHH
FDRFYRVEKSRNNKIGGSGLGLAITKNIIELHEGTIKAYSNDNKTVFEIKLPIKININSN
HHHHHHHHHCCCCCCCCCCCCHHHHHHHHHHCCCEEEEECCCCCEEEEEEEEEEEEECCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 8380148; 1556077 [H]