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The map label for this gene is barA [H]

Identifier: 226947749

GI number: 226947749

Start: 645202

End: 646449

Strand: Direct

Name: barA [H]

Synonym: CLM_0598

Alternate gene names: 226947749

Gene position: 645202-646449 (Clockwise)

Preceding gene: 226947748

Following gene: 226947750

Centisome position: 15.53

GC content: 24.12

Gene sequence:

>1248_bases
ATGGTAAAGTATGGGGATATAAATGCTTTGAATTTATTTGTGGGGGAGTTAAATAACGTTTCTATATTCATTTTGGATAA
AGATTTTCATTATATAAAATTAAATAATAGTCATAAAAAGTTTATGGAAAAATTATTAGGAATTCATGTAGAAGTGGGTA
TGAATATATTAGAAATAATTGAAGAATATTGTCCCCTAAACATTAGTGATAATTTAATATTGAGAATAAAAGAAAAAATG
AAGAGTGCATTAAAGGGCAATAAAATTATAACAAATGAAGAAGTTTTAATTGACGGTAGTAAAGTAGAATTTTATGAAAT
AGAGATAATTCCTTTAAAGGATGAAGATAACAATATATTAGGAGTAGGTGCATGTTGCTTAAATATAACAGAGAATGTAA
ATATAATGATAGAAGTATTAAAAACTAAATTAGATTTAACCTATAAAAAACATTTAAAAAATAGTGAATTATTAAATGGG
GAAAATAGTAAGGAAAAGATATTTAAAATATTGATAGCAGAGGATAATAATGTGAATCAAATAGTAATGAGTAAATTAAT
TGAACTAAAGGGATGGGTGGCAAAAACAGTTTCTAACGGGAAAGAGTCCATAGTAGCTCTAGAAAAAGACAACTATGATT
TAATCTTTATGGATATATCAATGCCTGTAATGAATGGACTAGATGCAGCAAATATAATTAAAAAAAATCCTAAATGGAGT
AAAATACCTATAGTAGCTTTAACAGCATACGACTCTTTAGAGCAAAAGAAAAAATTTAAATCATTAGGAATGGATGATTA
TCTAAGTAAACCTATAGATTCAAATAAGCTGCAATACATAATAGATAAACATTTAAATAATAATATACAAATTGAGAATA
ATAAAGTAAAGCTAAAGAATAAAGAAATATATAGTAGTTTTGAACGATTAGAAAAAGATTTAGATGGTAATAAAGAGCTA
GTAATAGAATTGGCTCATAAAATAATAAAATTATTTTCTAAAGAACAAATGAATGAAATTATGAGGTTATCAAGAGATGG
AGATATAGAAAATTTAAGAAATTTAATACATAAATTAAAGGGAGCCTGCTCAAATTTTGCTTTATATGAGGTAAGAAAAT
TGCTAAATGAAATTAAGGAAAGAGCTATAGTAGGTGATATTGAACATATTTATAAATTGGTAGAAAAAGTAACAATAAAT
ATGAATAAATTAGAGAAAGATTTGATAGATTATAGTAAAAATAAATAA

Upstream 100 bases:

>100_bases
TTTTATAATATATGAATATGTATAAGTTTTCCGTAGAAACTTCGAAAATCATAATAGTATGCTTAAAATAATAATATATT
TATAGGGAAGGATAAAATTT

Downstream 100 bases:

>100_bases
ATTTATAAAAATATCTTTAATCTAGTTGAAATTTGGGAATTAATGATATAATATTAGAATATAAGCTAATATTAACAATA
TATGTGGAGAATAAGTATTT

Product: response regulator

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 415; Mature: 415

Protein sequence:

>415_residues
MVKYGDINALNLFVGELNNVSIFILDKDFHYIKLNNSHKKFMEKLLGIHVEVGMNILEIIEEYCPLNISDNLILRIKEKM
KSALKGNKIITNEEVLIDGSKVEFYEIEIIPLKDEDNNILGVGACCLNITENVNIMIEVLKTKLDLTYKKHLKNSELLNG
ENSKEKIFKILIAEDNNVNQIVMSKLIELKGWVAKTVSNGKESIVALEKDNYDLIFMDISMPVMNGLDAANIIKKNPKWS
KIPIVALTAYDSLEQKKKFKSLGMDDYLSKPIDSNKLQYIIDKHLNNNIQIENNKVKLKNKEIYSSFERLEKDLDGNKEL
VIELAHKIIKLFSKEQMNEIMRLSRDGDIENLRNLIHKLKGACSNFALYEVRKLLNEIKERAIVGDIEHIYKLVEKVTIN
MNKLEKDLIDYSKNK

Sequences:

>Translated_415_residues
MVKYGDINALNLFVGELNNVSIFILDKDFHYIKLNNSHKKFMEKLLGIHVEVGMNILEIIEEYCPLNISDNLILRIKEKM
KSALKGNKIITNEEVLIDGSKVEFYEIEIIPLKDEDNNILGVGACCLNITENVNIMIEVLKTKLDLTYKKHLKNSELLNG
ENSKEKIFKILIAEDNNVNQIVMSKLIELKGWVAKTVSNGKESIVALEKDNYDLIFMDISMPVMNGLDAANIIKKNPKWS
KIPIVALTAYDSLEQKKKFKSLGMDDYLSKPIDSNKLQYIIDKHLNNNIQIENNKVKLKNKEIYSSFERLEKDLDGNKEL
VIELAHKIIKLFSKEQMNEIMRLSRDGDIENLRNLIHKLKGACSNFALYEVRKLLNEIKERAIVGDIEHIYKLVEKVTIN
MNKLEKDLIDYSKNK
>Mature_415_residues
MVKYGDINALNLFVGELNNVSIFILDKDFHYIKLNNSHKKFMEKLLGIHVEVGMNILEIIEEYCPLNISDNLILRIKEKM
KSALKGNKIITNEEVLIDGSKVEFYEIEIIPLKDEDNNILGVGACCLNITENVNIMIEVLKTKLDLTYKKHLKNSELLNG
ENSKEKIFKILIAEDNNVNQIVMSKLIELKGWVAKTVSNGKESIVALEKDNYDLIFMDISMPVMNGLDAANIIKKNPKWS
KIPIVALTAYDSLEQKKKFKSLGMDDYLSKPIDSNKLQYIIDKHLNNNIQIENNKVKLKNKEIYSSFERLEKDLDGNKEL
VIELAHKIIKLFSKEQMNEIMRLSRDGDIENLRNLIHKLKGACSNFALYEVRKLLNEIKERAIVGDIEHIYKLVEKVTIN
MNKLEKDLIDYSKNK

Specific function: Member of the two-component regulatory system UvrY/BarA involved in the regulation of carbon metabolism via the CsrA/CsrB regulatory system. Phosphorylates UvrY, probably via a four-step phosphorelay [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 response regulatory domain [H]

Homologues:

Organism=Escherichia coli, GI1789149, Length=271, Percent_Identity=24.3542435424354, Blast_Score=88, Evalue=1e-18,
Organism=Escherichia coli, GI48994928, Length=218, Percent_Identity=28.4403669724771, Blast_Score=72, Evalue=6e-14,
Organism=Escherichia coli, GI87082012, Length=110, Percent_Identity=32.7272727272727, Blast_Score=65, Evalue=7e-12,
Organism=Saccharomyces cerevisiae, GI6322044, Length=123, Percent_Identity=35.7723577235772, Blast_Score=71, Evalue=3e-13,
Organism=Saccharomyces cerevisiae, GI6322000, Length=153, Percent_Identity=30.0653594771242, Blast_Score=68, Evalue=3e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003594
- InterPro:   IPR011006
- InterPro:   IPR019247
- InterPro:   IPR003660
- InterPro:   IPR004358
- InterPro:   IPR008207
- InterPro:   IPR003661
- InterPro:   IPR005467
- InterPro:   IPR009082
- InterPro:   IPR001789 [H]

Pfam domain/function: PF09984 DUF2222; PF00672 HAMP; PF02518 HATPase_c; PF00512 HisKA; PF01627 Hpt; PF00072 Response_reg [H]

EC number: =2.7.13.3 [H]

Molecular weight: Translated: 47829; Mature: 47829

Theoretical pI: Translated: 7.60; Mature: 7.60

Prosite motif: PS50110 RESPONSE_REGULATORY

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
3.1 %Met     (Translated Protein)
4.1 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
3.1 %Met     (Mature Protein)
4.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MVKYGDINALNLFVGELNNVSIFILDKDFHYIKLNNSHKKFMEKLLGIHVEVGMNILEII
CCEECCCCEEEEEEECCCCEEEEEEECCEEEEEECCCHHHHHHHHHCEEHHHCCHHHHHH
EEYCPLNISDNLILRIKEKMKSALKGNKIITNEEVLIDGSKVEFYEIEIIPLKDEDNNIL
HHHCCCCCCCCHHEEEHHHHHHHHCCCEEEECCEEEEECCEEEEEEEEEEEECCCCCCEE
GVGACCLNITENVNIMIEVLKTKLDLTYKKHLKNSELLNGENSKEKIFKILIAEDNNVNQ
EEEHEEEECCCCCHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCHHEEEEEEEECCCCHHH
IVMSKLIELKGWVAKTVSNGKESIVALEKDNYDLIFMDISMPVMNGLDAANIIKKNPKWS
HHHHHHHHHHHHHHHHHCCCCCEEEEEECCCCEEEEEEECCHHHCCCCHHHHHHCCCCCC
KIPIVALTAYDSLEQKKKFKSLGMDDYLSKPIDSNKLQYIIDKHLNNNIQIENNKVKLKN
CCCEEEEECHHHHHHHHHHHHCCCHHHHCCCCCCCCHHHHHHHHCCCCEEEECCEEEECC
KEIYSSFERLEKDLDGNKELVIELAHKIIKLFSKEQMNEIMRLSRDGDIENLRNLIHKLK
HHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHH
GACSNFALYEVRKLLNEIKERAIVGDIEHIYKLVEKVTINMNKLEKDLIDYSKNK
HHHCCHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHCCHHHHHHHHHHCCCCC
>Mature Secondary Structure
MVKYGDINALNLFVGELNNVSIFILDKDFHYIKLNNSHKKFMEKLLGIHVEVGMNILEII
CCEECCCCEEEEEEECCCCEEEEEEECCEEEEEECCCHHHHHHHHHCEEHHHCCHHHHHH
EEYCPLNISDNLILRIKEKMKSALKGNKIITNEEVLIDGSKVEFYEIEIIPLKDEDNNIL
HHHCCCCCCCCHHEEEHHHHHHHHCCCEEEECCEEEEECCEEEEEEEEEEEECCCCCCEE
GVGACCLNITENVNIMIEVLKTKLDLTYKKHLKNSELLNGENSKEKIFKILIAEDNNVNQ
EEEHEEEECCCCCHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCHHEEEEEEEECCCCHHH
IVMSKLIELKGWVAKTVSNGKESIVALEKDNYDLIFMDISMPVMNGLDAANIIKKNPKWS
HHHHHHHHHHHHHHHHHCCCCCEEEEEECCCCEEEEEEECCHHHCCCCHHHHHHCCCCCC
KIPIVALTAYDSLEQKKKFKSLGMDDYLSKPIDSNKLQYIIDKHLNNNIQIENNKVKLKN
CCCEEEEECHHHHHHHHHHHHCCCHHHHCCCCCCCCHHHHHHHHCCCCEEEECCEEEECC
KEIYSSFERLEKDLDGNKELVIELAHKIIKLFSKEQMNEIMRLSRDGDIENLRNLIHKLK
HHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHH
GACSNFALYEVRKLLNEIKERAIVGDIEHIYKLVEKVTINMNKLEKDLIDYSKNK
HHHCCHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHCCHHHHHHHHHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]