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The map label for this gene is trmB

Identifier: 226947742

GI number: 226947742

Start: 637380

End: 638033

Strand: Direct

Name: trmB

Synonym: CLM_0591

Alternate gene names: 226947742

Gene position: 637380-638033 (Clockwise)

Preceding gene: 226947738

Following gene: 226947743

Centisome position: 15.34

GC content: 24.62

Gene sequence:

>654_bases
ATGAGACTTAGAAAAAAATGGTGGGCAAGACCGGAGATAGAAGCTAGTGATAAATTTGCAGAGGAACCTAAAGAATTAAG
AGGAAAATGGAATAAGGAATTTAATAATAATAATGATATACATTTAGAATTAGGTTGTGGAAGAGGAGGATTTATATCAC
AATTAGTAGAAAAAAATAAAGATATAAATTATGTAGGAATAGATTTAAAGGATGAAGTTATAGTTTATGCTATAAGAAAA
GTTAAAGAAAAGGAAGAAGAAGTTAAAAGAGAGTTTAAAAATATAAAATTTGTTACAATGAATATAATGGGAATAGCAGA
AGTATTTGATAAAAATGAAATAAGTAAAATATATATAAATTTTTGCAACCCTTGGCCAAAAGAAAGGCATAATAAAAGAA
GATTAACCCATACAAAATTACTAACGGAATATAAAAAATTTTTAAAACCAAATACAGAAATATGGTTTAAAACTGATGAC
AAAGAACTTTTTGAGGATTCACAAGAATATTTTAAGGAAAGTGGATTTAATATAGAATACATCACATATGATCTTCATAA
TAGTGATTTTAAAGAGAATATAAAAACAGAATATGAAACAAAATTTGAAACTATGGGAATGAAAATAATGTTTTTAAAGG
CTAGATTATTATAA

Upstream 100 bases:

>100_bases
TTGTTTTTTTAAATATAATTAAATATAGATTACTAATAATAATAGGTGTTATGATATAATATTTCAGTAGTAGATTAAAA
AGATATCTGGAGGTTAAAAA

Downstream 100 bases:

>100_bases
AAGAATTTATATAGAATGATATTTATTATAAATTGTTTACTATAGCATAGATTTTGCGAAAGATGTTTTACAAGGGTAAA
GTGAGAGCTAATACATAAAG

Product: tRNA (guanine-N(7)-)-methyltransferase

Products: NA

Alternate protein names: tRNA(m7G46)-methyltransferase

Number of amino acids: Translated: 217; Mature: 217

Protein sequence:

>217_residues
MRLRKKWWARPEIEASDKFAEEPKELRGKWNKEFNNNNDIHLELGCGRGGFISQLVEKNKDINYVGIDLKDEVIVYAIRK
VKEKEEEVKREFKNIKFVTMNIMGIAEVFDKNEISKIYINFCNPWPKERHNKRRLTHTKLLTEYKKFLKPNTEIWFKTDD
KELFEDSQEYFKESGFNIEYITYDLHNSDFKENIKTEYETKFETMGMKIMFLKARLL

Sequences:

>Translated_217_residues
MRLRKKWWARPEIEASDKFAEEPKELRGKWNKEFNNNNDIHLELGCGRGGFISQLVEKNKDINYVGIDLKDEVIVYAIRK
VKEKEEEVKREFKNIKFVTMNIMGIAEVFDKNEISKIYINFCNPWPKERHNKRRLTHTKLLTEYKKFLKPNTEIWFKTDD
KELFEDSQEYFKESGFNIEYITYDLHNSDFKENIKTEYETKFETMGMKIMFLKARLL
>Mature_217_residues
MRLRKKWWARPEIEASDKFAEEPKELRGKWNKEFNNNNDIHLELGCGRGGFISQLVEKNKDINYVGIDLKDEVIVYAIRK
VKEKEEEVKREFKNIKFVTMNIMGIAEVFDKNEISKIYINFCNPWPKERHNKRRLTHTKLLTEYKKFLKPNTEIWFKTDD
KELFEDSQEYFKESGFNIEYITYDLHNSDFKENIKTEYETKFETMGMKIMFLKARLL

Specific function: Catalyzes the formation of N(7)-methylguanine at position 46 (m7G46) in tRNA

COG id: COG0220

COG function: function code R; Predicted S-adenosylmethionine-dependent methyltransferase

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the methyltransferase superfamily. TrmB family

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): TRMB_CLOB1 (A7FRB3)

Other databases:

- EMBL:   CP000726
- RefSeq:   YP_001382890.1
- ProteinModelPortal:   A7FRB3
- SMR:   A7FRB3
- STRING:   A7FRB3
- GeneID:   5395158
- GenomeReviews:   CP000726_GR
- KEGG:   cba:CLB_0540
- eggNOG:   COG0220
- HOGENOM:   HBG304014
- OMA:   LRNKPWA
- ProtClustDB:   PRK00121
- BioCyc:   CBOT441770:CLB_0540-MONOMER
- HAMAP:   MF_01057
- InterPro:   IPR003358
- PANTHER:   PTHR23417:SF1
- TIGRFAMs:   TIGR00091

Pfam domain/function: PF02390 Methyltransf_4

EC number: =2.1.1.33

Molecular weight: Translated: 26259; Mature: 26259

Theoretical pI: Translated: 8.92; Mature: 8.92

Prosite motif: NA

Important sites: BINDING 43-43 BINDING 68-68 BINDING 101-101 BINDING 123-123 BINDING 127-127 BINDING 159-159

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
2.8 %Met     (Translated Protein)
3.7 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
2.8 %Met     (Mature Protein)
3.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRLRKKWWARPEIEASDKFAEEPKELRGKWNKEFNNNNDIHLELGCGRGGFISQLVEKNK
CCCCCHHCCCCCCCCCHHHHCCHHHHHCCCCCCCCCCCCEEEEEECCCCHHHHHHHHCCC
DINYVGIDLKDEVIVYAIRKVKEKEEEVKREFKNIKFVTMNIMGIAEVFDKNEISKIYIN
CCCEEECCCCHHHHHHHHHHHHHHHHHHHHHHHCCEEEEEEHHHHHHHHCCCCCEEEEEE
FCNPWPKERHNKRRLTHTKLLTEYKKFLKPNTEIWFKTDDKELFEDSQEYFKESGFNIEY
CCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCEEEEECCCHHHHHHHHHHHHHCCCCEEE
ITYDLHNSDFKENIKTEYETKFETMGMKIMFLKARLL
EEEECCCCCHHHHHHHHHHHHHHHHCEEEEEEEECCC
>Mature Secondary Structure
MRLRKKWWARPEIEASDKFAEEPKELRGKWNKEFNNNNDIHLELGCGRGGFISQLVEKNK
CCCCCHHCCCCCCCCCHHHHCCHHHHHCCCCCCCCCCCCEEEEEECCCCHHHHHHHHCCC
DINYVGIDLKDEVIVYAIRKVKEKEEEVKREFKNIKFVTMNIMGIAEVFDKNEISKIYIN
CCCEEECCCCHHHHHHHHHHHHHHHHHHHHHHHCCEEEEEEHHHHHHHHCCCCCEEEEEE
FCNPWPKERHNKRRLTHTKLLTEYKKFLKPNTEIWFKTDDKELFEDSQEYFKESGFNIEY
CCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCEEEEECCCHHHHHHHHHHHHHCCCCEEE
ITYDLHNSDFKENIKTEYETKFETMGMKIMFLKARLL
EEEECCCCCHHHHHHHHHHHHHHHHCEEEEEEEECCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA