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The map label for this gene is thiM [H]

Identifier: 226947686

GI number: 226947686

Start: 579015

End: 579806

Strand: Reverse

Name: thiM [H]

Synonym: CLM_0532

Alternate gene names: 226947686

Gene position: 579806-579015 (Counterclockwise)

Preceding gene: 226947687

Following gene: 226947685

Centisome position: 13.95

GC content: 33.59

Gene sequence:

>792_bases
ATGGAAAATAAAAATGTTATACAAAAAATGAGAGAAAAAACTCCACTTATTCACTGCATAACTAACTATGTTACTATAAA
CGATTGTGCTAATATACTTCTTTCCTTTGGTGCTTCTCCTGCTATGTGTGAAGCCTATGACGAAGTTTATGATTTTGTAT
CTATATCTTCTGCTTTATATATAAATTTAGGAACTTTAACTAAAGAGCAGGAGACTGCAGCCGTACTTGCTTCTATCTCT
GCCAAAAATCATAATGTACCTGTAGTTATAGACCCTGTAGGATGCCCTGCTATAAAAAGAAAAGTTGAAGTTATAAATAG
AATCGCTGAAGTTGGAAGAATAGATATTATCAAAGGAAACATAGGAGAAATTAAATTTTTAGCAGGAATGGATTCTGAAA
CTAGAGGGGTAGATTCTCTAGATAATGGTGAAAATGCTTTAGATGCTTGCACACAATTAGCAAAAAAATATAATTGCATT
GTGGCCGCTACTGGCCAAAAAGATTTTGTATCCGATGGGAAAAGAGGTTCTGTAATAAAAAATGGTACAGAAATGCTAAC
CAAAGTAACTGGAGCTGGTTGTATGCTAGGTGCCCTATGTGCCGCTACCTGCGCAAATTTTGAAGATAAATTAGTATCTA
CTACAGCTGCCATATTATCTATGAACATTGCTGGAGAAAAAGCATACGAAAAAGCACAACTACCAGGTAGTTTTAGAATT
GCTTTAATAGATAATATATATATGATATCTGATAAAGAAATTTGGGAAAGAGGTAATGTAGAATGGAAATAA

Upstream 100 bases:

>100_bases
AGAAAATAAAAATTTTATAAAATTTATTAATTAAGCAAAAAAACATAAACAATCTTTTTTACTAAAACTATATATAATTT
AAATAAAAGGAGCGATTTAT

Downstream 100 bases:

>100_bases
ATTATGAACTTTATTTAATTACAGATAGAAGATTTTTAAAAGGACGTCAATTAAAAAAAATAGTGGAAGACGCTATCCTA
GGAGGAGTAACTATAGTACA

Product: hydroxyethylthiazole kinase

Products: NA

Alternate protein names: 4-methyl-5-beta-hydroxyethylthiazole kinase 1; TH kinase 1; Thz kinase 1 [H]

Number of amino acids: Translated: 263; Mature: 263

Protein sequence:

>263_residues
MENKNVIQKMREKTPLIHCITNYVTINDCANILLSFGASPAMCEAYDEVYDFVSISSALYINLGTLTKEQETAAVLASIS
AKNHNVPVVIDPVGCPAIKRKVEVINRIAEVGRIDIIKGNIGEIKFLAGMDSETRGVDSLDNGENALDACTQLAKKYNCI
VAATGQKDFVSDGKRGSVIKNGTEMLTKVTGAGCMLGALCAATCANFEDKLVSTTAAILSMNIAGEKAYEKAQLPGSFRI
ALIDNIYMISDKEIWERGNVEWK

Sequences:

>Translated_263_residues
MENKNVIQKMREKTPLIHCITNYVTINDCANILLSFGASPAMCEAYDEVYDFVSISSALYINLGTLTKEQETAAVLASIS
AKNHNVPVVIDPVGCPAIKRKVEVINRIAEVGRIDIIKGNIGEIKFLAGMDSETRGVDSLDNGENALDACTQLAKKYNCI
VAATGQKDFVSDGKRGSVIKNGTEMLTKVTGAGCMLGALCAATCANFEDKLVSTTAAILSMNIAGEKAYEKAQLPGSFRI
ALIDNIYMISDKEIWERGNVEWK
>Mature_263_residues
MENKNVIQKMREKTPLIHCITNYVTINDCANILLSFGASPAMCEAYDEVYDFVSISSALYINLGTLTKEQETAAVLASIS
AKNHNVPVVIDPVGCPAIKRKVEVINRIAEVGRIDIIKGNIGEIKFLAGMDSETRGVDSLDNGENALDACTQLAKKYNCI
VAATGQKDFVSDGKRGSVIKNGTEMLTKVTGAGCMLGALCAATCANFEDKLVSTTAAILSMNIAGEKAYEKAQLPGSFRI
ALIDNIYMISDKEIWERGNVEWK

Specific function: Thiamine biosynthesis. [C]

COG id: COG2145

COG function: function code H; Hydroxyethylthiazole kinase, sugar kinase family

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the Thz kinase family [H]

Homologues:

Organism=Escherichia coli, GI1788421, Length=251, Percent_Identity=36.6533864541833, Blast_Score=157, Evalue=6e-40,
Organism=Saccharomyces cerevisiae, GI6325042, Length=294, Percent_Identity=31.2925170068027, Blast_Score=103, Evalue=4e-23,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000417 [H]

Pfam domain/function: PF02110 HK [H]

EC number: =2.7.1.50 [H]

Molecular weight: Translated: 28453; Mature: 28453

Theoretical pI: Translated: 5.18; Mature: 5.18

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

3.4 %Cys     (Translated Protein)
3.0 %Met     (Translated Protein)
6.5 %Cys+Met (Translated Protein)
3.4 %Cys     (Mature Protein)
3.0 %Met     (Mature Protein)
6.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MENKNVIQKMREKTPLIHCITNYVTINDCANILLSFGASPAMCEAYDEVYDFVSISSALY
CCCHHHHHHHHHCCCEEEHHHHCEEHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHCCEEE
INLGTLTKEQETAAVLASISAKNHNVPVVIDPVGCPAIKRKVEVINRIAEVGRIDIIKGN
EEECCCCCHHHHHHHHHHHHCCCCCCCEEECCCCCHHHHHHHHHHHHHHHCCCEEEEECC
IGEIKFLAGMDSETRGVDSLDNGENALDACTQLAKKYNCIVAATGQKDFVSDGKRGSVIK
CCCEEEEECCCCCCCCCCCCCCCCHHHHHHHHHHHHCCEEEEECCCCHHHCCCCCCCEEC
NGTEMLTKVTGAGCMLGALCAATCANFEDKLVSTTAAILSMNIAGEKAYEKAQLPGSFRI
CHHHHHHHHHCCHHHHHHHHHHHHCCHHHHHHHHHHHHHHCCCCCHHHHHHHCCCCCEEE
ALIDNIYMISDKEIWERGNVEWK
EEEEEEEEECCHHHHHCCCCCCC
>Mature Secondary Structure
MENKNVIQKMREKTPLIHCITNYVTINDCANILLSFGASPAMCEAYDEVYDFVSISSALY
CCCHHHHHHHHHCCCEEEHHHHCEEHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHCCEEE
INLGTLTKEQETAAVLASISAKNHNVPVVIDPVGCPAIKRKVEVINRIAEVGRIDIIKGN
EEECCCCCHHHHHHHHHHHHCCCCCCCEEECCCCCHHHHHHHHHHHHHHHCCCEEEEECC
IGEIKFLAGMDSETRGVDSLDNGENALDACTQLAKKYNCIVAATGQKDFVSDGKRGSVIK
CCCEEEEECCCCCCCCCCCCCCCCHHHHHHHHHHHHCCEEEEECCCCHHHCCCCCCCEEC
NGTEMLTKVTGAGCMLGALCAATCANFEDKLVSTTAAILSMNIAGEKAYEKAQLPGSFRI
CHHHHHHHHHCCHHHHHHHHHHHHCCHHHHHHHHHHHHHHCCCCCHHHHHHHCCCCCEEE
ALIDNIYMISDKEIWERGNVEWK
EEEEEEEEECCHHHHHCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA