Click here to switch to the map view.

The map label for this gene is thiD [H]

Identifier: 226947687

GI number: 226947687

Start: 580037

End: 580837

Strand: Reverse

Name: thiD [H]

Synonym: CLM_0533

Alternate gene names: 226947687

Gene position: 580837-580037 (Counterclockwise)

Preceding gene: 226947705

Following gene: 226947686

Centisome position: 13.98

GC content: 31.71

Gene sequence:

>801_bases
ATGAAAAAAGTTTTAACTATAGCCGGTTCGGATAGTTGTGGAGGTGCTGGCATTCAAGCAGATATTAAAACTATGAGTTC
TTTAGGAGTATATGGTATGAGCATAATAACTGCTGTAACCGCTCAAAATTCCATAGGTGTACAAGATGTTCATGAAGTGC
CAAAGAACATGGTTGAAGCCCAAATCAAATCTGTATTTGATGATATAGATGTAGATGCTGTAAAAATAGGTATGCTGTCC
AATTCTGAAACTATAAAATCTATAAAAGAATACTTAGAAAAATATAAAGCTAAAAATATAGTTTTAGATCCTGTTATGGT
TTCTAAAAGTGGTTATTTTCTTCTTAAGCCAGAGGCTATAGAAGAACTAAAAAAATTAATTTCTATAACTAACATTGTAA
CCCCTAATATACCAGAGGCTGAAGTTCTGAGTAAAATAGAAATCAATTCAGAAGATGATATGAAGAAAGCTGCTACTATA
ATCCAAGCTATTGGAGTTAAAAACGTTTTAGTTAAAGGTGGCCATAGATGCAGTGATGCAAATGACATACTTCTTTATGA
AGATAAATTTATAACAATCCCTGGTAACAGAATAGAAACAAAAAATACTCATGGTACTGGCTGTACATTATCCTCTGCTA
TAGCTTCTTATTTAGCTAAAGGCTTTAACATAGAAAAATCAGTATCTTTATCTAAAGAATATATTACAAAAGCTATCGAG
AATTCCTTCCCTATTGGTGAAGGCGTAGGTCCTGTTGGACATTTTATAGAGCTTTATAAAAAAGCACATTTAGATTTTTA
A

Upstream 100 bases:

>100_bases
TTAAACTCACTTAAAATAAATCTATTATTATTAAATAACATTGACTTTACTATTGCAGAAACAAACTTAAATTAATTAAT
TACCTAGGAGGTTTTTTATC

Downstream 100 bases:

>100_bases
GAATTTCTTCTGTGATACTATTTAAGTTTAATTAATAAGAATCTATAAATTAAACTTAAAAACATGGACAATCATTATTA
TGGTAAAGCTACATGAAATT

Product: phosphomethylpyrimidine kinase

Products: NA

Alternate protein names: Thiamine-phosphate pyrophosphorylase; TMP pyrophosphorylase; TMP-PPase; Thiamine-phosphate synthase; Hydroxymethylpyrimidine/phosphomethylpyrimidine kinase; Hydroxymethylpyrimidine kinase; HMP kinase; Hydroxymethylpyrimidine phosphate kinase; HMP-P kinase; HMP-phosphate kinase; HMPP kinase [H]

Number of amino acids: Translated: 266; Mature: 266

Protein sequence:

>266_residues
MKKVLTIAGSDSCGGAGIQADIKTMSSLGVYGMSIITAVTAQNSIGVQDVHEVPKNMVEAQIKSVFDDIDVDAVKIGMLS
NSETIKSIKEYLEKYKAKNIVLDPVMVSKSGYFLLKPEAIEELKKLISITNIVTPNIPEAEVLSKIEINSEDDMKKAATI
IQAIGVKNVLVKGGHRCSDANDILLYEDKFITIPGNRIETKNTHGTGCTLSSAIASYLAKGFNIEKSVSLSKEYITKAIE
NSFPIGEGVGPVGHFIELYKKAHLDF

Sequences:

>Translated_266_residues
MKKVLTIAGSDSCGGAGIQADIKTMSSLGVYGMSIITAVTAQNSIGVQDVHEVPKNMVEAQIKSVFDDIDVDAVKIGMLS
NSETIKSIKEYLEKYKAKNIVLDPVMVSKSGYFLLKPEAIEELKKLISITNIVTPNIPEAEVLSKIEINSEDDMKKAATI
IQAIGVKNVLVKGGHRCSDANDILLYEDKFITIPGNRIETKNTHGTGCTLSSAIASYLAKGFNIEKSVSLSKEYITKAIE
NSFPIGEGVGPVGHFIELYKKAHLDF
>Mature_266_residues
MKKVLTIAGSDSCGGAGIQADIKTMSSLGVYGMSIITAVTAQNSIGVQDVHEVPKNMVEAQIKSVFDDIDVDAVKIGMLS
NSETIKSIKEYLEKYKAKNIVLDPVMVSKSGYFLLKPEAIEELKKLISITNIVTPNIPEAEVLSKIEINSEDDMKKAATI
IQAIGVKNVLVKGGHRCSDANDILLYEDKFITIPGNRIETKNTHGTGCTLSSAIASYLAKGFNIEKSVSLSKEYITKAIE
NSFPIGEGVGPVGHFIELYKKAHLDF

Specific function: Catalyzes the phosphorylation of hydroxymethylpyrimidine phosphate (HMP-P) to HMP-PP, and of HMP to HMP-P [H]

COG id: COG0351

COG function: function code H; Hydroxymethylpyrimidine/phosphomethylpyrimidine kinase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: In the C-terminal section; belongs to the thiD family [H]

Homologues:

Organism=Escherichia coli, GI1788420, Length=269, Percent_Identity=42.3791821561338, Blast_Score=220, Evalue=6e-59,
Organism=Saccharomyces cerevisiae, GI6325378, Length=271, Percent_Identity=34.6863468634686, Blast_Score=142, Evalue=6e-35,
Organism=Saccharomyces cerevisiae, GI6324517, Length=276, Percent_Identity=36.231884057971, Blast_Score=121, Evalue=1e-28,
Organism=Saccharomyces cerevisiae, GI6324997, Length=276, Percent_Identity=36.231884057971, Blast_Score=120, Evalue=2e-28,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013785
- InterPro:   IPR013749
- InterPro:   IPR004399
- InterPro:   IPR022998
- InterPro:   IPR003733 [H]

Pfam domain/function: PF08543 Phos_pyr_kin; PF02581 TMP-TENI [H]

EC number: =2.5.1.3; =2.7.1.49; =2.7.4.7 [H]

Molecular weight: Translated: 28784; Mature: 28784

Theoretical pI: Translated: 6.37; Mature: 6.37

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
2.6 %Met     (Translated Protein)
3.8 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
2.6 %Met     (Mature Protein)
3.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKKVLTIAGSDSCGGAGIQADIKTMSSLGVYGMSIITAVTAQNSIGVQDVHEVPKNMVEA
CCCEEEEECCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHH
QIKSVFDDIDVDAVKIGMLSNSETIKSIKEYLEKYKAKNIVLDPVMVSKSGYFLLKPEAI
HHHHHHHCCCCCEEEEEECCCHHHHHHHHHHHHHHHHCCEEECCEEECCCCEEEECHHHH
EELKKLISITNIVTPNIPEAEVLSKIEINSEDDMKKAATIIQAIGVKNVLVKGGHRCSDA
HHHHHHHHHHHHCCCCCCHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHCCCCCCCCC
NDILLYEDKFITIPGNRIETKNTHGTGCTLSSAIASYLAKGFNIEKSVSLSKEYITKAIE
CCEEEEECEEEECCCCEEECCCCCCCCCHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHH
NSFPIGEGVGPVGHFIELYKKAHLDF
CCCCCCCCCCHHHHHHHHHHHHCCCC
>Mature Secondary Structure
MKKVLTIAGSDSCGGAGIQADIKTMSSLGVYGMSIITAVTAQNSIGVQDVHEVPKNMVEA
CCCEEEEECCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHH
QIKSVFDDIDVDAVKIGMLSNSETIKSIKEYLEKYKAKNIVLDPVMVSKSGYFLLKPEAI
HHHHHHHCCCCCEEEEEECCCHHHHHHHHHHHHHHHHCCEEECCEEECCCCEEEECHHHH
EELKKLISITNIVTPNIPEAEVLSKIEINSEDDMKKAATIIQAIGVKNVLVKGGHRCSDA
HHHHHHHHHHHHCCCCCCHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHCCCCCCCCC
NDILLYEDKFITIPGNRIETKNTHGTGCTLSSAIASYLAKGFNIEKSVSLSKEYITKAIE
CCEEEEECEEEECCCCEEECCCCCCCCCHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHH
NSFPIGEGVGPVGHFIELYKKAHLDF
CCCCCCCCCCHHHHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA