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The map label for this gene is thiE [H]

Identifier: 226947685

GI number: 226947685

Start: 578407

End: 579024

Strand: Reverse

Name: thiE [H]

Synonym: CLM_0531

Alternate gene names: 226947685

Gene position: 579024-578407 (Counterclockwise)

Preceding gene: 226947686

Following gene: 226947684

Centisome position: 13.93

GC content: 30.58

Gene sequence:

>618_bases
ATGGAAATAAATTATGAACTTTATTTAATTACAGATAGAAGATTTTTAAAAGGACGTCAATTAAAAAAAATAGTGGAAGA
CGCTATCCTAGGAGGAGTAACTATAGTACAAGTTAGAGAAAAAGATGTATCTACTAGAGAATTTTATAATGTAGCTAAAG
AAGTAAAGGAAGTAACAGACTATTACAAGGTGCCTATAATTATAAATGATAGATTAGATATTGCTCAAGCTATAGATGCT
AGCGGCGTTCATCTGGGTCAAAAGGACATGCATCTTAATATAGCTAGAGAAATTTTAGGCAAGGATAAAATAATAGGCAT
ATCTGTAGGAAATGTTAAAGAGGCCTTGGAAGCACAAAATAATGGAGCAGATTATCTAGGAATAGGAACTATATTCCCTA
CAGGTAGCAAGAAAGATGTAGATGCAATCATTGGTATTGATGGACTATCAAAAATAAAAGATAGCATATCTATCCCTTCT
GTAGCCATAGGTGGCATAAATAAAACTAACTTTAAAGATGTTTTAAAAACTGGCATAGAAGGAATTTCTGTAATTTCAGC
TATATTAGATGAAGATGATATAAAACTTGCTGCTAACAATCTTTTAATAAATAAATAA

Upstream 100 bases:

>100_bases
CATACGAAAAAGCACAACTACCAGGTAGTTTTAGAATTGCTTTAATAGATAATATATATATGATATCTGATAAAGAAATT
TGGGAAAGAGGTAATGTAGA

Downstream 100 bases:

>100_bases
TTACCTTTATTTGTTATTTTAACTGAAAATAGATTTAATTTTAATATAACAAAGATAAAAAATATCAGTGTATTTTTTAT
TCTGGCAATGTTAAAATTAA

Product: thiamine-phosphate pyrophosphorylase

Products: NA

Alternate protein names: TMP pyrophosphorylase; TMP-PPase; Thiamine-phosphate synthase [H]

Number of amino acids: Translated: 205; Mature: 205

Protein sequence:

>205_residues
MEINYELYLITDRRFLKGRQLKKIVEDAILGGVTIVQVREKDVSTREFYNVAKEVKEVTDYYKVPIIINDRLDIAQAIDA
SGVHLGQKDMHLNIAREILGKDKIIGISVGNVKEALEAQNNGADYLGIGTIFPTGSKKDVDAIIGIDGLSKIKDSISIPS
VAIGGINKTNFKDVLKTGIEGISVISAILDEDDIKLAANNLLINK

Sequences:

>Translated_205_residues
MEINYELYLITDRRFLKGRQLKKIVEDAILGGVTIVQVREKDVSTREFYNVAKEVKEVTDYYKVPIIINDRLDIAQAIDA
SGVHLGQKDMHLNIAREILGKDKIIGISVGNVKEALEAQNNGADYLGIGTIFPTGSKKDVDAIIGIDGLSKIKDSISIPS
VAIGGINKTNFKDVLKTGIEGISVISAILDEDDIKLAANNLLINK
>Mature_205_residues
MEINYELYLITDRRFLKGRQLKKIVEDAILGGVTIVQVREKDVSTREFYNVAKEVKEVTDYYKVPIIINDRLDIAQAIDA
SGVHLGQKDMHLNIAREILGKDKIIGISVGNVKEALEAQNNGADYLGIGTIFPTGSKKDVDAIIGIDGLSKIKDSISIPS
VAIGGINKTNFKDVLKTGIEGISVISAILDEDDIKLAANNLLINK

Specific function: Condenses 4-methyl-5-(beta-hydroxyethyl)thiazole monophosphate (THZ-P) and 2-methyl-4-amino-5-hydroxymethyl pyrimidine pyrophosphate (HMP-PP) to form thiamine monophosphate (TMP) [H]

COG id: COG0352

COG function: function code H; Thiamine monophosphate synthase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the TMP-PPase family [H]

Homologues:

Organism=Escherichia coli, GI1790426, Length=184, Percent_Identity=30.9782608695652, Blast_Score=76, Evalue=1e-15,
Organism=Saccharomyces cerevisiae, GI6325042, Length=220, Percent_Identity=40.4545454545455, Blast_Score=149, Evalue=3e-37,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013785
- InterPro:   IPR022998
- InterPro:   IPR003733 [H]

Pfam domain/function: PF02581 TMP-TENI [H]

EC number: =2.5.1.3 [H]

Molecular weight: Translated: 22421; Mature: 22421

Theoretical pI: Translated: 5.40; Mature: 5.40

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
1.0 %Met     (Translated Protein)
1.0 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
1.0 %Met     (Mature Protein)
1.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MEINYELYLITDRRFLKGRQLKKIVEDAILGGVTIVQVREKDVSTREFYNVAKEVKEVTD
CCCEEEEEEEECCHHHHHHHHHHHHHHHHHCCEEEEEEECCCCCHHHHHHHHHHHHHHHH
YYKVPIIINDRLDIAQAIDASGVHLGQKDMHLNIAREILGKDKIIGISVGNVKEALEAQN
HEECCEEEECCCHHHHHHCCCCCCCCCCHHHHHHHHHHHCCCCEEEEECCCHHHHHHHCC
NGADYLGIGTIFPTGSKKDVDAIIGIDGLSKIKDSISIPSVAIGGINKTNFKDVLKTGIE
CCCCEEEEEEEECCCCCCCCCEEEECCCHHHHHHHCCCCCEEECCCCCCCHHHHHHHCCH
GISVISAILDEDDIKLAANNLLINK
HHHHHHHHHCCCCCEEEECEEEECC
>Mature Secondary Structure
MEINYELYLITDRRFLKGRQLKKIVEDAILGGVTIVQVREKDVSTREFYNVAKEVKEVTD
CCCEEEEEEEECCHHHHHHHHHHHHHHHHHCCEEEEEEECCCCCHHHHHHHHHHHHHHHH
YYKVPIIINDRLDIAQAIDASGVHLGQKDMHLNIAREILGKDKIIGISVGNVKEALEAQN
HEECCEEEECCCHHHHHHCCCCCCCCCCHHHHHHHHHHHCCCCEEEEECCCHHHHHHHCC
NGADYLGIGTIFPTGSKKDVDAIIGIDGLSKIKDSISIPSVAIGGINKTNFKDVLKTGIE
CCCCEEEEEEEECCCCCCCCCEEEECCCHHHHHHHCCCCCEEECCCCCCCHHHHHHHCCH
GISVISAILDEDDIKLAANNLLINK
HHHHHHHHHCCCCCEEEECEEEECC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA