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The map label for this gene is leuC2 [H]

Identifier: 226947654

GI number: 226947654

Start: 537690

End: 539615

Strand: Direct

Name: leuC2 [H]

Synonym: CLM_0497

Alternate gene names: 226947654

Gene position: 537690-539615 (Clockwise)

Preceding gene: 226947653

Following gene: 226947655

Centisome position: 12.94

GC content: 31.72

Gene sequence:

>1926_bases
TTGGGTTTAACATTAACAGAAAAGATAATAAAATCACATTTAGTAAAAGGAAATATGACTAAAGGAGAAGAAATTGCTAT
TAAAATAGATAATACTTTAACTCAGGATTCTACAGGAACTATGGCCTATCTTCAGTTTGAAGCTTTAGGTATAGATAAAG
TAAAGACTAAAAGATCAGTGGCTTATATAGACCACAATATGTTGCAAAGTGGACCAGAGAATGCGGATGATCATTTATAT
ATACAAACAGTAGCTAAAAAACATGGAATTTATTTTTCAAAACCAGGTAATGGAATATGTCATCAAGTTAATCTGGAAAG
ATTTGGTGTGCCTGGAGAAACTTTATTAGGATCCGATAGCCATACTCCAACAGGAGGAGGTATAGGAATGTTAGCTATAG
GTGCTGGTGGATTGGATGTAGCAGTAGCTATGGCAGGTGGAGAATATTATATAAATATGCCTAAAGTAGTTAAGGTAAAT
TTAATAGGAAAATTAAAACCTTTTGTATCTGCAAAAGATATTATCTTAGAAGTTTTAAGAAGATTATCTGTAAAGGGTGG
AGTAGGAAAAATATTTGAATATACAGGAGAAGGAGTAAAAGCTCTTACAGTTCCAGAGAGAGCAACTATAACTAATATGG
GAGCAGAACTTGGAGCTACTACATCAATTTTTCCATCAGATGAAATTACTAAAGATTTCTTAAAAGCTCAAGAAAGAGAA
GAAGATTATAAGTATCTAGTGGCAGATGAGGATGCTGTTTATGATGAGGAATTAGAAATAAATTTATCAGATCTAAAACC
TTTAGCAGCCTGTCCACACAGCCCTGATAATATAGTACCTGTATCAGAACTTAAAAATATAAAAGTTAACCAAGTAGCTA
TAGGTAGTTGTACAAATTCTTCTTATAGAGATATGTTAAGAACAGCTAGTATATTAAAGGGGAAAACTGTATCAGAAAAT
GTATCTTTAGTTATAGCACCAGGCTCTAAACAAGTTCTAAACATGATGGCTAATAATGGAGCTTTAACAGATTTAGTTAT
GGCAGGAGCAAGAATACTAGAATGTGCCTGTGGACCATGTATAGGAATGGGACAATCTCCTTGTACAGATGCTGTATCTT
TAAGAACTTTTAATAGAAATTTTGAGGGTAGATCTGGAACAAAATCTGCTAATGTGTATTTAGTAAGTCCAGAAGTGGCA
GCAGTTTCAGCATTAACAGGATATATAACAGATCCCACTGAGTTAACTGATTTTGAAGATATAGAAATGCCTAATAAATT
TTTGATAAATGATAATTTAATAATAGAACCAGCTACAGAAGGAGAAGTTGTAGATGTTAAAAGAGGACCAAATATAAAAC
CTTTCCCTAAAGCAGAAGCTTTAAAAGAATTAGTAACTGGAAAGGCATTAACTAAAGTAGGGGACAATATAACAACAGAT
CATATAATGCCATCTAATGCTAAACTTTTACCTTATAGATCTAATGTACCATATTTATCAAACTTTTGCTTAACTCCTTG
TGATGAAGAATTTCCTGAAAAAGCTAAGGAAAATGGAGGAGGTATAATAGTCGCAGGAACAAATTACGGACAAGGTTCTA
GTAGAGAACATGCAGCCTTAGCTCCCGTTTATTTAGGAATAAAAGCAGTTTTAGCAAAATCCTTTGCTAGAATTCATAAA
GCAAATCTAATAAATAATGGTATATTACCTTTAGTTTTTGAAAGAGAAGAAGATTATGATTCTATAGATGAAATGGATGA
ATTAGTAATAGAAAATGTACTAAATCAGGTGGAAATAGGTAAAATAGATATAATAAATAAAACTAAGGGTATAAAATATA
AAACTTTATTAGAAGCAACAGAGAGACAAAAAAAGATGATTTTAAAGGGCGGATTATTAAATTTAATAAAAAGTTCTCAA
CAATAG

Upstream 100 bases:

>100_bases
AAACCCTATAAATAAAAACTAAAGAGAGTATAAATAAAAAATTAGTAAGTAAAAAACAAAAGATAAATATTAAAGTATAA
ATTGATTGGGGGAATAACAG

Downstream 100 bases:

>100_bases
CTACAAAAATTTAGAATTAGAATAAATATGGATAGTAACAAATTATTATTAACATGTTTAAATAAATATAACTTATAGGG
AAGTATATATATAATAATAG

Product: aconitate hydratase

Products: NA

Alternate protein names: Alpha-IPM isomerase 2; IPMI 2; Isopropylmalate isomerase 2 [H]

Number of amino acids: Translated: 641; Mature: 640

Protein sequence:

>641_residues
MGLTLTEKIIKSHLVKGNMTKGEEIAIKIDNTLTQDSTGTMAYLQFEALGIDKVKTKRSVAYIDHNMLQSGPENADDHLY
IQTVAKKHGIYFSKPGNGICHQVNLERFGVPGETLLGSDSHTPTGGGIGMLAIGAGGLDVAVAMAGGEYYINMPKVVKVN
LIGKLKPFVSAKDIILEVLRRLSVKGGVGKIFEYTGEGVKALTVPERATITNMGAELGATTSIFPSDEITKDFLKAQERE
EDYKYLVADEDAVYDEELEINLSDLKPLAACPHSPDNIVPVSELKNIKVNQVAIGSCTNSSYRDMLRTASILKGKTVSEN
VSLVIAPGSKQVLNMMANNGALTDLVMAGARILECACGPCIGMGQSPCTDAVSLRTFNRNFEGRSGTKSANVYLVSPEVA
AVSALTGYITDPTELTDFEDIEMPNKFLINDNLIIEPATEGEVVDVKRGPNIKPFPKAEALKELVTGKALTKVGDNITTD
HIMPSNAKLLPYRSNVPYLSNFCLTPCDEEFPEKAKENGGGIIVAGTNYGQGSSREHAALAPVYLGIKAVLAKSFARIHK
ANLINNGILPLVFEREEDYDSIDEMDELVIENVLNQVEIGKIDIINKTKGIKYKTLLEATERQKKMILKGGLLNLIKSSQ
Q

Sequences:

>Translated_641_residues
MGLTLTEKIIKSHLVKGNMTKGEEIAIKIDNTLTQDSTGTMAYLQFEALGIDKVKTKRSVAYIDHNMLQSGPENADDHLY
IQTVAKKHGIYFSKPGNGICHQVNLERFGVPGETLLGSDSHTPTGGGIGMLAIGAGGLDVAVAMAGGEYYINMPKVVKVN
LIGKLKPFVSAKDIILEVLRRLSVKGGVGKIFEYTGEGVKALTVPERATITNMGAELGATTSIFPSDEITKDFLKAQERE
EDYKYLVADEDAVYDEELEINLSDLKPLAACPHSPDNIVPVSELKNIKVNQVAIGSCTNSSYRDMLRTASILKGKTVSEN
VSLVIAPGSKQVLNMMANNGALTDLVMAGARILECACGPCIGMGQSPCTDAVSLRTFNRNFEGRSGTKSANVYLVSPEVA
AVSALTGYITDPTELTDFEDIEMPNKFLINDNLIIEPATEGEVVDVKRGPNIKPFPKAEALKELVTGKALTKVGDNITTD
HIMPSNAKLLPYRSNVPYLSNFCLTPCDEEFPEKAKENGGGIIVAGTNYGQGSSREHAALAPVYLGIKAVLAKSFARIHK
ANLINNGILPLVFEREEDYDSIDEMDELVIENVLNQVEIGKIDIINKTKGIKYKTLLEATERQKKMILKGGLLNLIKSSQ
Q
>Mature_640_residues
GLTLTEKIIKSHLVKGNMTKGEEIAIKIDNTLTQDSTGTMAYLQFEALGIDKVKTKRSVAYIDHNMLQSGPENADDHLYI
QTVAKKHGIYFSKPGNGICHQVNLERFGVPGETLLGSDSHTPTGGGIGMLAIGAGGLDVAVAMAGGEYYINMPKVVKVNL
IGKLKPFVSAKDIILEVLRRLSVKGGVGKIFEYTGEGVKALTVPERATITNMGAELGATTSIFPSDEITKDFLKAQEREE
DYKYLVADEDAVYDEELEINLSDLKPLAACPHSPDNIVPVSELKNIKVNQVAIGSCTNSSYRDMLRTASILKGKTVSENV
SLVIAPGSKQVLNMMANNGALTDLVMAGARILECACGPCIGMGQSPCTDAVSLRTFNRNFEGRSGTKSANVYLVSPEVAA
VSALTGYITDPTELTDFEDIEMPNKFLINDNLIIEPATEGEVVDVKRGPNIKPFPKAEALKELVTGKALTKVGDNITTDH
IMPSNAKLLPYRSNVPYLSNFCLTPCDEEFPEKAKENGGGIIVAGTNYGQGSSREHAALAPVYLGIKAVLAKSFARIHKA
NLINNGILPLVFEREEDYDSIDEMDELVIENVLNQVEIGKIDIINKTKGIKYKTLLEATERQKKMILKGGLLNLIKSSQQ

Specific function: Catalyzes the isomerization between 2-isopropylmalate and 3-isopropylmalate, via the formation of 2-isopropylmaleate [H]

COG id: COG1048

COG function: function code C; Aconitase A

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the aconitase/IPM isomerase family. LeuC type 2 subfamily [H]

Homologues:

Organism=Homo sapiens, GI4501867, Length=722, Percent_Identity=35.3185595567867, Blast_Score=321, Evalue=2e-87,
Organism=Homo sapiens, GI8659555, Length=732, Percent_Identity=27.0491803278689, Blast_Score=199, Evalue=6e-51,
Organism=Homo sapiens, GI41352693, Length=660, Percent_Identity=27.5757575757576, Blast_Score=181, Evalue=1e-45,
Organism=Escherichia coli, GI87081781, Length=727, Percent_Identity=27.3727647867951, Blast_Score=237, Evalue=1e-63,
Organism=Escherichia coli, GI1786259, Length=466, Percent_Identity=28.3261802575107, Blast_Score=158, Evalue=1e-39,
Organism=Escherichia coli, GI1787531, Length=633, Percent_Identity=27.3301737756714, Blast_Score=144, Evalue=1e-35,
Organism=Escherichia coli, GI2367097, Length=461, Percent_Identity=25.8134490238612, Blast_Score=95, Evalue=1e-20,
Organism=Caenorhabditis elegans, GI25149337, Length=716, Percent_Identity=35.7541899441341, Blast_Score=322, Evalue=3e-88,
Organism=Caenorhabditis elegans, GI32564738, Length=680, Percent_Identity=36.1764705882353, Blast_Score=313, Evalue=2e-85,
Organism=Caenorhabditis elegans, GI25149342, Length=566, Percent_Identity=35.6890459363958, Blast_Score=258, Evalue=1e-68,
Organism=Caenorhabditis elegans, GI17568399, Length=826, Percent_Identity=25.7869249394673, Blast_Score=186, Evalue=3e-47,
Organism=Saccharomyces cerevisiae, GI6322261, Length=712, Percent_Identity=34.1292134831461, Blast_Score=344, Evalue=3e-95,
Organism=Saccharomyces cerevisiae, GI6323335, Length=728, Percent_Identity=32.967032967033, Blast_Score=326, Evalue=8e-90,
Organism=Saccharomyces cerevisiae, GI6320440, Length=611, Percent_Identity=32.8968903436989, Blast_Score=245, Evalue=1e-65,
Organism=Saccharomyces cerevisiae, GI6321429, Length=670, Percent_Identity=27.3134328358209, Blast_Score=192, Evalue=1e-49,
Organism=Drosophila melanogaster, GI281365315, Length=673, Percent_Identity=36.1069836552749, Blast_Score=317, Evalue=2e-86,
Organism=Drosophila melanogaster, GI17864292, Length=673, Percent_Identity=36.1069836552749, Blast_Score=317, Evalue=2e-86,
Organism=Drosophila melanogaster, GI28571643, Length=720, Percent_Identity=34.3055555555556, Blast_Score=315, Evalue=6e-86,
Organism=Drosophila melanogaster, GI161076999, Length=637, Percent_Identity=36.734693877551, Blast_Score=305, Evalue=6e-83,
Organism=Drosophila melanogaster, GI24645686, Length=759, Percent_Identity=26.0869565217391, Blast_Score=172, Evalue=6e-43,
Organism=Drosophila melanogaster, GI17137564, Length=724, Percent_Identity=26.9337016574586, Blast_Score=167, Evalue=2e-41,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR015931
- InterPro:   IPR015937
- InterPro:   IPR001030
- InterPro:   IPR015932
- InterPro:   IPR018136
- InterPro:   IPR011826
- InterPro:   IPR015936
- InterPro:   IPR006251 [H]

Pfam domain/function: PF00330 Aconitase [H]

EC number: =4.2.1.33 [H]

Molecular weight: Translated: 69385; Mature: 69253

Theoretical pI: Translated: 5.47; Mature: 5.47

Prosite motif: PS00450 ACONITASE_1 ; PS01244 ACONITASE_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.4 %Cys     (Translated Protein)
2.7 %Met     (Translated Protein)
4.1 %Cys+Met (Translated Protein)
1.4 %Cys     (Mature Protein)
2.5 %Met     (Mature Protein)
3.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MGLTLTEKIIKSHLVKGNMTKGEEIAIKIDNTLTQDSTGTMAYLQFEALGIDKVKTKRSV
CCCCHHHHHHHHHHHCCCCCCCCEEEEEECCCCCCCCCCCEEEEEEEECCCCHHHHCCCE
AYIDHNMLQSGPENADDHLYIQTVAKKHGIYFSKPGNGICHQVNLERFGVPGETLLGSDS
EEEEHHHHHCCCCCCCCEEEEEEEHHHCCEEEECCCCCCEEEECHHHCCCCCCCEECCCC
HTPTGGGIGMLAIGAGGLDVAVAMAGGEYYINMPKVVKVNLIGKLKPFVSAKDIILEVLR
CCCCCCCEEEEEEECCCCEEEEEEECCEEEEECCCEEEEEEEECCCCCCCHHHHHHHHHH
RLSVKGGVGKIFEYTGEGVKALTVPERATITNMGAELGATTSIFPSDEITKDFLKAQERE
HHCCCCCCCHHHHCCCCCEEEEECCCCHHHHHCCHHHCCCCCCCCCHHHHHHHHHHHHCC
EDYKYLVADEDAVYDEELEINLSDLKPLAACPHSPDNIVPVSELKNIKVNQVAIGSCTNS
CCCEEEEECCCCCCCCEEEEEHHHCCHHHCCCCCCCCEEEHHHHCCCEEEEEEEECCCCH
SYRDMLRTASILKGKTVSENVSLVIAPGSKQVLNMMANNGALTDLVMAGARILECACGPC
HHHHHHHHHHHHCCCEECCCCEEEEECCCHHHHHHHHCCCCHHHHHHHHHHHHHHHCCCC
IGMGQSPCTDAVSLRTFNRNFEGRSGTKSANVYLVSPEVAAVSALTGYITDPTELTDFED
CCCCCCCCCCHHEEEEECCCCCCCCCCCCCEEEEECCCHHHHHHHHCCCCCCCCCCCCHH
IEMPNKFLINDNLIIEPATEGEVVDVKRGPNIKPFPKAEALKELVTGKALTKVGDNITTD
CCCCCEEEECCCEEEEECCCCCEEEECCCCCCCCCCHHHHHHHHHCCHHHHHCCCCCCCC
HIMPSNAKLLPYRSNVPYLSNFCLTPCDEEFPEKAKENGGGIIVAGTNYGQGSSREHAAL
CCCCCCCEEEEECCCCCHHHCCCCCCCCHHHHHHHHHCCCEEEEECCCCCCCCCCCCCCH
APVYLGIKAVLAKSFARIHKANLINNGILPLVFEREEDYDSIDEMDELVIENVLNQVEIG
HHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEEECCCCCCHHHHHHHHHHHHHHHHCCCC
KIDIINKTKGIKYKTLLEATERQKKMILKGGLLNLIKSSQQ
EEEEEECCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCC
>Mature Secondary Structure 
GLTLTEKIIKSHLVKGNMTKGEEIAIKIDNTLTQDSTGTMAYLQFEALGIDKVKTKRSV
CCCHHHHHHHHHHHCCCCCCCCEEEEEECCCCCCCCCCCEEEEEEEECCCCHHHHCCCE
AYIDHNMLQSGPENADDHLYIQTVAKKHGIYFSKPGNGICHQVNLERFGVPGETLLGSDS
EEEEHHHHHCCCCCCCCEEEEEEEHHHCCEEEECCCCCCEEEECHHHCCCCCCCEECCCC
HTPTGGGIGMLAIGAGGLDVAVAMAGGEYYINMPKVVKVNLIGKLKPFVSAKDIILEVLR
CCCCCCCEEEEEEECCCCEEEEEEECCEEEEECCCEEEEEEEECCCCCCCHHHHHHHHHH
RLSVKGGVGKIFEYTGEGVKALTVPERATITNMGAELGATTSIFPSDEITKDFLKAQERE
HHCCCCCCCHHHHCCCCCEEEEECCCCHHHHHCCHHHCCCCCCCCCHHHHHHHHHHHHCC
EDYKYLVADEDAVYDEELEINLSDLKPLAACPHSPDNIVPVSELKNIKVNQVAIGSCTNS
CCCEEEEECCCCCCCCEEEEEHHHCCHHHCCCCCCCCEEEHHHHCCCEEEEEEEECCCCH
SYRDMLRTASILKGKTVSENVSLVIAPGSKQVLNMMANNGALTDLVMAGARILECACGPC
HHHHHHHHHHHHCCCEECCCCEEEEECCCHHHHHHHHCCCCHHHHHHHHHHHHHHHCCCC
IGMGQSPCTDAVSLRTFNRNFEGRSGTKSANVYLVSPEVAAVSALTGYITDPTELTDFED
CCCCCCCCCCHHEEEEECCCCCCCCCCCCCEEEEECCCHHHHHHHHCCCCCCCCCCCCHH
IEMPNKFLINDNLIIEPATEGEVVDVKRGPNIKPFPKAEALKELVTGKALTKVGDNITTD
CCCCCEEEECCCEEEEECCCCCEEEECCCCCCCCCCHHHHHHHHHCCHHHHHCCCCCCCC
HIMPSNAKLLPYRSNVPYLSNFCLTPCDEEFPEKAKENGGGIIVAGTNYGQGSSREHAAL
CCCCCCCEEEEECCCCCHHHCCCCCCCCHHHHHHHHHCCCEEEEECCCCCCCCCCCCCCH
APVYLGIKAVLAKSFARIHKANLINNGILPLVFEREEDYDSIDEMDELVIENVLNQVEIG
HHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEEECCCCCCHHHHHHHHHHHHHHHHCCCC
KIDIINKTKGIKYKTLLEATERQKKMILKGGLLNLIKSSQQ
EEEEEECCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 9389475 [H]