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The map label for this gene is leuA2 [H]

Identifier: 226947653

GI number: 226947653

Start: 536185

End: 537546

Strand: Direct

Name: leuA2 [H]

Synonym: CLM_0496

Alternate gene names: 226947653

Gene position: 536185-537546 (Clockwise)

Preceding gene: 226947652

Following gene: 226947654

Centisome position: 12.9

GC content: 31.2

Gene sequence:

>1362_bases
ATGATGGTTAAAATAGTTAAGTCTTCATATAATCATGTACTTCAAAATATATCAAGTCCTAATTTGTATAAGGATCTATT
TCCATATGATGAAATACCAAAGGTGACATTTAATGATATACAGGTTCCAATGGATTTACCAAAGGATATATGGATTACTG
ATACAACATTTAGAGATGGACAACAATCAATGCCACCATATACTACAGAACAAATAGTAAAAATATATGACTATCTTCAT
AAATTAGACAATGAATCTGGGGTAATAAGACAAACAGAATTCTTCTTGTATTCTGAGAAAGACAGAAAGGCAGCAGAAAT
ATGTTTAGATAGAGGCTATAAGTTTCCAGAAGTTACTTCATGGATAAGAGCTAGCAAAGAAGATTTTAAATTGGTTAAGG
AAATGGGAATAAAAGAAACTGGCATGCTTATGTCTTGTTCAGATTACCATATATTTAAGAAACTTAATACAACAAGAGAA
AAGGCTATGAGTATATATTTAGAAATCGCGGAAGAAGCTTTAGCTAATGGAATTGTTCCAAGATGCCATTTAGAAGATAT
AACAAGAGCGGATTTTTATGGGTTTGTAGTTCCTTTTGTAAATAAATTGATGGAACTCTCCAATAAAGCTAATATACCTG
TTAAAATAAGAGCCTGTGACACTTTAGGATTAGGGGTTCCTTATAGTGGAGCATCACTACCAAGAAGTGTTCAAGGAATC
ATTCATGGACTTAGATATTGTTGTAATGTTCCTTCAGAAAGAATAGAGTGGCATGGACACAATGATTTTTATGGTGTAGT
AGCTAATGCTTCCACATCATGGCTTTATGGCTGCTCATCTGTAAATACTTCTCTTTTAGGTATAGGAGAAAGAACGGGAA
ATTGTCCTCTAGAAGCTATGATTATAGAATATGGACAATTAAAAGGTAATACCGCTAATATGAACTTACATGTTATAAAT
GAAATAGCAGAATATTTTGAGAGAGAAATGAATTATAATATACCATCAAGAACACCTTTTGTAGGAAGTGAGTTTAATGT
TACAAGAGCTGGTATTCATGCAGATGGTATATTAAAAAATGAGGAAATATATAATATTTTCAATACAGAAAAGATTTTAG
ACAGACCAGTTGTAGTTGCAGTAAATCAATATTCAGGCTTAGCTGGTATTGCAGCTTGGATAAACACTTACTTTAAATTA
AAAGGTGAAAATAAAATAGATAAAAAAGATGAAAGAATTGTGTCTATAAAAGAATGGGTAGATAATCAATATGAAAATGG
CAGAACAACAATAATAGGAAATAATGAATTGGAAGTTATAGTAAAGCAACTTATACCGGATTTATTTAGAAGAGCTAGCT
AG

Upstream 100 bases:

>100_bases
TAAATATTATCTAAAATGAAGGAAAAGAATTAAGAAATGCATAATATAGTTTTTTGAGTAAAATATGGGGGAAAACCAAT
AAATAAATTTAGGGGGAATT

Downstream 100 bases:

>100_bases
TAATTACCATATAATTTCATAAAATAAAAAAAATATTTAAATAAAACCCTATAAATAAAAACTAAAGAGAGTATAAATAA
AAAATTAGTAAGTAAAAAAC

Product: HMGL-related enzyme

Products: NA

Alternate protein names: Alpha-IPM synthase 2; Alpha-isopropylmalate synthase 2 [H]

Number of amino acids: Translated: 453; Mature: 453

Protein sequence:

>453_residues
MMVKIVKSSYNHVLQNISSPNLYKDLFPYDEIPKVTFNDIQVPMDLPKDIWITDTTFRDGQQSMPPYTTEQIVKIYDYLH
KLDNESGVIRQTEFFLYSEKDRKAAEICLDRGYKFPEVTSWIRASKEDFKLVKEMGIKETGMLMSCSDYHIFKKLNTTRE
KAMSIYLEIAEEALANGIVPRCHLEDITRADFYGFVVPFVNKLMELSNKANIPVKIRACDTLGLGVPYSGASLPRSVQGI
IHGLRYCCNVPSERIEWHGHNDFYGVVANASTSWLYGCSSVNTSLLGIGERTGNCPLEAMIIEYGQLKGNTANMNLHVIN
EIAEYFEREMNYNIPSRTPFVGSEFNVTRAGIHADGILKNEEIYNIFNTEKILDRPVVVAVNQYSGLAGIAAWINTYFKL
KGENKIDKKDERIVSIKEWVDNQYENGRTTIIGNNELEVIVKQLIPDLFRRAS

Sequences:

>Translated_453_residues
MMVKIVKSSYNHVLQNISSPNLYKDLFPYDEIPKVTFNDIQVPMDLPKDIWITDTTFRDGQQSMPPYTTEQIVKIYDYLH
KLDNESGVIRQTEFFLYSEKDRKAAEICLDRGYKFPEVTSWIRASKEDFKLVKEMGIKETGMLMSCSDYHIFKKLNTTRE
KAMSIYLEIAEEALANGIVPRCHLEDITRADFYGFVVPFVNKLMELSNKANIPVKIRACDTLGLGVPYSGASLPRSVQGI
IHGLRYCCNVPSERIEWHGHNDFYGVVANASTSWLYGCSSVNTSLLGIGERTGNCPLEAMIIEYGQLKGNTANMNLHVIN
EIAEYFEREMNYNIPSRTPFVGSEFNVTRAGIHADGILKNEEIYNIFNTEKILDRPVVVAVNQYSGLAGIAAWINTYFKL
KGENKIDKKDERIVSIKEWVDNQYENGRTTIIGNNELEVIVKQLIPDLFRRAS
>Mature_453_residues
MMVKIVKSSYNHVLQNISSPNLYKDLFPYDEIPKVTFNDIQVPMDLPKDIWITDTTFRDGQQSMPPYTTEQIVKIYDYLH
KLDNESGVIRQTEFFLYSEKDRKAAEICLDRGYKFPEVTSWIRASKEDFKLVKEMGIKETGMLMSCSDYHIFKKLNTTRE
KAMSIYLEIAEEALANGIVPRCHLEDITRADFYGFVVPFVNKLMELSNKANIPVKIRACDTLGLGVPYSGASLPRSVQGI
IHGLRYCCNVPSERIEWHGHNDFYGVVANASTSWLYGCSSVNTSLLGIGERTGNCPLEAMIIEYGQLKGNTANMNLHVIN
EIAEYFEREMNYNIPSRTPFVGSEFNVTRAGIHADGILKNEEIYNIFNTEKILDRPVVVAVNQYSGLAGIAAWINTYFKL
KGENKIDKKDERIVSIKEWVDNQYENGRTTIIGNNELEVIVKQLIPDLFRRAS

Specific function: Catalyzes the condensation of the acetyl group of acetyl-CoA with 3-methyl-2-oxobutanoate (2-oxoisovalerate) to form 3-carboxy-3-hydroxy-4-methylpentanoate (2-isopropylmalate) [H]

COG id: COG0119

COG function: function code E; Isopropylmalate/homocitrate/citramalate synthases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the alpha-IPM synthase/homocitrate synthase family. LeuA type 1 subfamily [H]

Homologues:

Organism=Escherichia coli, GI1786261, Length=363, Percent_Identity=26.9972451790634, Blast_Score=89, Evalue=5e-19,
Organism=Saccharomyces cerevisiae, GI6320019, Length=351, Percent_Identity=25.3561253561254, Blast_Score=87, Evalue=8e-18,
Organism=Saccharomyces cerevisiae, GI6320071, Length=355, Percent_Identity=24.7887323943662, Blast_Score=86, Evalue=1e-17,

Paralogues:

None

Copy number: 160 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002034
- InterPro:   IPR013785
- InterPro:   IPR013477
- InterPro:   IPR000891 [H]

Pfam domain/function: PF00682 HMGL-like [H]

EC number: =2.3.3.13 [H]

Molecular weight: Translated: 51675; Mature: 51675

Theoretical pI: Translated: 6.08; Mature: 6.08

Prosite motif: PS50991 PYR_CT

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.8 %Cys     (Translated Protein)
2.6 %Met     (Translated Protein)
4.4 %Cys+Met (Translated Protein)
1.8 %Cys     (Mature Protein)
2.6 %Met     (Mature Protein)
4.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MMVKIVKSSYNHVLQNISSPNLYKDLFPYDEIPKVTFNDIQVPMDLPKDIWITDTTFRDG
CEEEEEHHHHHHHHHCCCCCCHHHHHCCCCCCCCCCCCCEEECCCCCCCEEEECCCCCCC
QQSMPPYTTEQIVKIYDYLHKLDNESGVIRQTEFFLYSEKDRKAAEICLDRGYKFPEVTS
CCCCCCCCHHHHHHHHHHHHHHCCCCCCEEEEEEEEEECCCHHHHHHHHHCCCCCHHHHH
WIRASKEDFKLVKEMGIKETGMLMSCSDYHIFKKLNTTREKAMSIYLEIAEEALANGIVP
HHHCCHHHHHHHHHCCCCCCCCEEECCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCC
RCHLEDITRADFYGFVVPFVNKLMELSNKANIPVKIRACDTLGLGVPYSGASLPRSVQGI
CCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEEEECEECCCCCCCCCCCCHHHHHH
IHGLRYCCNVPSERIEWHGHNDFYGVVANASTSWLYGCSSVNTSLLGIGERTGNCPLEAM
HHHHHHHHCCCHHHEEECCCCCEEEEEECCCCCEEEECCCCCCEEEECCCCCCCCCHHHH
IIEYGQLKGNTANMNLHVINEIAEYFEREMNYNIPSRTPFVGSEFNVTRAGIHADGILKN
EEHHHCCCCCCCCEEEHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCEEECCCCCCCCCCC
EEIYNIFNTEKILDRPVVVAVNQYSGLAGIAAWINTYFKLKGENKIDKKDERIVSIKEWV
CHHHHHHCHHHHHCCCEEEEEECCCCHHHHHHHHHHHEEECCCCCCCCHHHHHHHHHHHH
DNQYENGRTTIIGNNELEVIVKQLIPDLFRRAS
CCCCCCCCEEEEECCHHHHHHHHHHHHHHHHCC
>Mature Secondary Structure
MMVKIVKSSYNHVLQNISSPNLYKDLFPYDEIPKVTFNDIQVPMDLPKDIWITDTTFRDG
CEEEEEHHHHHHHHHCCCCCCHHHHHCCCCCCCCCCCCCEEECCCCCCCEEEECCCCCCC
QQSMPPYTTEQIVKIYDYLHKLDNESGVIRQTEFFLYSEKDRKAAEICLDRGYKFPEVTS
CCCCCCCCHHHHHHHHHHHHHHCCCCCCEEEEEEEEEECCCHHHHHHHHHCCCCCHHHHH
WIRASKEDFKLVKEMGIKETGMLMSCSDYHIFKKLNTTREKAMSIYLEIAEEALANGIVP
HHHCCHHHHHHHHHCCCCCCCCEEECCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCC
RCHLEDITRADFYGFVVPFVNKLMELSNKANIPVKIRACDTLGLGVPYSGASLPRSVQGI
CCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEEEECEECCCCCCCCCCCCHHHHHH
IHGLRYCCNVPSERIEWHGHNDFYGVVANASTSWLYGCSSVNTSLLGIGERTGNCPLEAM
HHHHHHHHCCCHHHEEECCCCCEEEEEECCCCCEEEECCCCCCEEEECCCCCCCCCHHHH
IIEYGQLKGNTANMNLHVINEIAEYFEREMNYNIPSRTPFVGSEFNVTRAGIHADGILKN
EEHHHCCCCCCCCEEEHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCEEECCCCCCCCCCC
EEIYNIFNTEKILDRPVVVAVNQYSGLAGIAAWINTYFKLKGENKIDKKDERIVSIKEWV
CHHHHHHCHHHHHCCCEEEEEECCCCHHHHHHHHHHHEEECCCCCCCCHHHHHHHHHHHH
DNQYENGRTTIIGNNELEVIVKQLIPDLFRRAS
CCCCCCCCEEEEECCHHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11997336 [H]