Click here to switch to the map view.

The map label for this gene is ycjM [H]

Identifier: 226225390

GI number: 226225390

Start: 2895553

End: 2897253

Strand: Reverse

Name: ycjM [H]

Synonym: Lm4b_02813

Alternate gene names: 226225390

Gene position: 2897253-2895553 (Counterclockwise)

Preceding gene: 226225392

Following gene: 226225389

Centisome position: 99.47

GC content: 40.27

Gene sequence:

>1701_bases
ATGACGAATTTAAGAAAACGACTTTCGCGTTTATATGCCGAAGATGTGGTAGATAGTTTGGCAGCTAGAATCGAAGCGCG
GGTACAGCAAACGCAACAACGAAAGTTAACACGAAAAGATCAGTGGGACGAGAAAGATATTGTTTTAATTACATATGGGG
ACCAATTTAAAGAGGAATCCAAAAAAACGTTAAAAACTTTTAAGAAAATGTATGATAGTTATTTAAAATCGACTTTTGAG
GTAGTTCATTTCTTGCCTTTTTATCCTTATTCTTCGGATGATGGGTTTTCGGTCATCGATTATAAAGCGGTGAACCCGGA
ACTTGGTGATTGGGAAGATATTAAGGAGATGGAACAGTCGGCGCGGTTGATGTTTGATTTTGTTTGTAATCATATGTCGG
CGAAAAGTGATTGGTTTAAGCGCTATTTGGCGGGTGACGAGGAGTTTAGGAACTTTTTTGTGGAAATGGATCCAAAGACG
GACCTTAGTTCGGTAACAAGGCCACGTGCGACACCAGTGCTGACACCATTTCAATTTGATTCAGGAAAAGTAGGGCATAT
TTGGACGACTTTTAGTGAGGATCAAATCGATTTAAACTTTGCTTCTCCGGAAGTGCTTTACAAGATGATTGATGTTTTGA
TGTTCTATTTAGAAGAAGGTGCGGAATATGTGCGACTTGATGCGGTTGGCTTTATGTGGAAAGTGCCGGGAACGAGCTCG
ATTCATTTAGAAGAGACGCATGAAATCGTGAAATTATTTAGAGATTTAGTAGACATTGCAGCTCCGGGGACGATTATTAT
AACCGAAACCAATGTGCCGCATGTTGATAATATTAGTTATTTTGGAAATGGTGAAAAAGAAGCGCATATGGTTTATCAAT
TCCCGCTTCCACCACTTGTGCTCCATGCGATACATCATGGTAATGCAGAATTCCTCCGTAACTGGGCGAAGAATTTAGAG
CTACCAGAAGGCAAGCGAACATTCTTTAATTTCCTTGCCTCACATGATGGGATTGGGTTAAATCCAGTGCGCGGCATTAT
TCCAGAAGCTGAAATTTTGGCTTTAGTGAATGATTTGGAGGAAGAAGGCGCGCTCGTTTCATATAAGCAAAATCCAGATG
GTTCTAAGAGTCCGTATGAAATTAACGTGACGTACATGGATGCGCTAAGTAAACAGGCGGACACAGATGACTTGAGGATG
TCGAGATTTCTTGTGGCTCATGCAGTACTAATGTCTATCCCAGGCGTTCCAGCCGTTTATGTTCAAAGTATTTTAGGAAG
CCGCAATGATTATTCAGGCGTAGAAACAACAGGCCACAATCGCTCTATTAATCGAAAAAAATACGACTTGGCGGAAATTA
CAGCAGAGTTGGAACAAAGAGGCTCCTTACGAAAAGCAACTTATGATGCGTTAACGAAATTAATTAGCACACGAAAAGCG
GAATCACTTTTCCATCCAGAAATACCGATGGAAGTTTTAGAATCTACGGCGGAATTGTTTGTTGTTAAACGTTCATCCGA
CGTGGAATCGATTATATTGATTCATAATTTATCAGAAAAAGAAGTGAGTTATTCACTAGACAGCGGTGTTTATACAAATC
TTTATACGGGCTCCACAGTAACTGGGAGCGATTCCATAAAGCTTAGTGGCTATGAATTCTGCTGGCTAAAAACCAAAAAT
TACAGGGAGGAACAAAAATGA

Upstream 100 bases:

>100_bases
ATCAACGTGTTAGAAAAAACATTAAAAATTGTGTTTGACAGAAAGCGCTTTCCATACTATAATTCTTCTTGTGGGAGCGC
TCCCGGTTAGGAGGTAAATG

Downstream 100 bases:

>100_bases
AGAAAAAGAGTCTTGTATTACTTGTTGTTGTGTTGGTTCTAAGTGCGGTTCTAGCTGCATGTGGAGGGAAAGAGTCTAGT
AGTAAGGAAGTAACGATTGA

Product: Sucrose phosphorylase

Products: NA

Alternate protein names: Sucrose glucosyltransferase [H]

Number of amino acids: Translated: 566; Mature: 565

Protein sequence:

>566_residues
MTNLRKRLSRLYAEDVVDSLAARIEARVQQTQQRKLTRKDQWDEKDIVLITYGDQFKEESKKTLKTFKKMYDSYLKSTFE
VVHFLPFYPYSSDDGFSVIDYKAVNPELGDWEDIKEMEQSARLMFDFVCNHMSAKSDWFKRYLAGDEEFRNFFVEMDPKT
DLSSVTRPRATPVLTPFQFDSGKVGHIWTTFSEDQIDLNFASPEVLYKMIDVLMFYLEEGAEYVRLDAVGFMWKVPGTSS
IHLEETHEIVKLFRDLVDIAAPGTIIITETNVPHVDNISYFGNGEKEAHMVYQFPLPPLVLHAIHHGNAEFLRNWAKNLE
LPEGKRTFFNFLASHDGIGLNPVRGIIPEAEILALVNDLEEEGALVSYKQNPDGSKSPYEINVTYMDALSKQADTDDLRM
SRFLVAHAVLMSIPGVPAVYVQSILGSRNDYSGVETTGHNRSINRKKYDLAEITAELEQRGSLRKATYDALTKLISTRKA
ESLFHPEIPMEVLESTAELFVVKRSSDVESIILIHNLSEKEVSYSLDSGVYTNLYTGSTVTGSDSIKLSGYEFCWLKTKN
YREEQK

Sequences:

>Translated_566_residues
MTNLRKRLSRLYAEDVVDSLAARIEARVQQTQQRKLTRKDQWDEKDIVLITYGDQFKEESKKTLKTFKKMYDSYLKSTFE
VVHFLPFYPYSSDDGFSVIDYKAVNPELGDWEDIKEMEQSARLMFDFVCNHMSAKSDWFKRYLAGDEEFRNFFVEMDPKT
DLSSVTRPRATPVLTPFQFDSGKVGHIWTTFSEDQIDLNFASPEVLYKMIDVLMFYLEEGAEYVRLDAVGFMWKVPGTSS
IHLEETHEIVKLFRDLVDIAAPGTIIITETNVPHVDNISYFGNGEKEAHMVYQFPLPPLVLHAIHHGNAEFLRNWAKNLE
LPEGKRTFFNFLASHDGIGLNPVRGIIPEAEILALVNDLEEEGALVSYKQNPDGSKSPYEINVTYMDALSKQADTDDLRM
SRFLVAHAVLMSIPGVPAVYVQSILGSRNDYSGVETTGHNRSINRKKYDLAEITAELEQRGSLRKATYDALTKLISTRKA
ESLFHPEIPMEVLESTAELFVVKRSSDVESIILIHNLSEKEVSYSLDSGVYTNLYTGSTVTGSDSIKLSGYEFCWLKTKN
YREEQK
>Mature_565_residues
TNLRKRLSRLYAEDVVDSLAARIEARVQQTQQRKLTRKDQWDEKDIVLITYGDQFKEESKKTLKTFKKMYDSYLKSTFEV
VHFLPFYPYSSDDGFSVIDYKAVNPELGDWEDIKEMEQSARLMFDFVCNHMSAKSDWFKRYLAGDEEFRNFFVEMDPKTD
LSSVTRPRATPVLTPFQFDSGKVGHIWTTFSEDQIDLNFASPEVLYKMIDVLMFYLEEGAEYVRLDAVGFMWKVPGTSSI
HLEETHEIVKLFRDLVDIAAPGTIIITETNVPHVDNISYFGNGEKEAHMVYQFPLPPLVLHAIHHGNAEFLRNWAKNLEL
PEGKRTFFNFLASHDGIGLNPVRGIIPEAEILALVNDLEEEGALVSYKQNPDGSKSPYEINVTYMDALSKQADTDDLRMS
RFLVAHAVLMSIPGVPAVYVQSILGSRNDYSGVETTGHNRSINRKKYDLAEITAELEQRGSLRKATYDALTKLISTRKAE
SLFHPEIPMEVLESTAELFVVKRSSDVESIILIHNLSEKEVSYSLDSGVYTNLYTGSTVTGSDSIKLSGYEFCWLKTKNY
REEQK

Specific function: Unknown

COG id: COG0366

COG function: function code G; Glycosidases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the sucrose phosphorylase family [H]

Homologues:

Organism=Homo sapiens, GI187423904, Length=331, Percent_Identity=25.0755287009063, Blast_Score=67, Evalue=5e-11,
Organism=Escherichia coli, GI87081873, Length=542, Percent_Identity=55.719557195572, Blast_Score=638, Evalue=0.0,
Organism=Escherichia coli, GI1790687, Length=221, Percent_Identity=23.9819004524887, Blast_Score=71, Evalue=2e-13,
Organism=Drosophila melanogaster, GI24586589, Length=297, Percent_Identity=27.6094276094276, Blast_Score=102, Evalue=9e-22,
Organism=Drosophila melanogaster, GI24586591, Length=147, Percent_Identity=32.6530612244898, Blast_Score=84, Evalue=2e-16,
Organism=Drosophila melanogaster, GI24583747, Length=240, Percent_Identity=26.25, Blast_Score=82, Evalue=7e-16,
Organism=Drosophila melanogaster, GI24583749, Length=240, Percent_Identity=26.25, Blast_Score=82, Evalue=7e-16,
Organism=Drosophila melanogaster, GI45549022, Length=291, Percent_Identity=23.7113402061856, Blast_Score=80, Evalue=3e-15,
Organism=Drosophila melanogaster, GI24586599, Length=366, Percent_Identity=23.4972677595628, Blast_Score=73, Evalue=6e-13,
Organism=Drosophila melanogaster, GI281360393, Length=343, Percent_Identity=23.9067055393586, Blast_Score=72, Evalue=1e-12,
Organism=Drosophila melanogaster, GI24586593, Length=346, Percent_Identity=23.4104046242775, Blast_Score=72, Evalue=1e-12,
Organism=Drosophila melanogaster, GI24583745, Length=217, Percent_Identity=25.8064516129032, Blast_Score=71, Evalue=2e-12,
Organism=Drosophila melanogaster, GI24586587, Length=219, Percent_Identity=26.027397260274, Blast_Score=66, Evalue=5e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR006047
- InterPro:   IPR017853
- InterPro:   IPR013781
- InterPro:   IPR016377 [H]

Pfam domain/function: PF00128 Alpha-amylase [H]

EC number: =2.4.1.7 [H]

Molecular weight: Translated: 64734; Mature: 64603

Theoretical pI: Translated: 5.04; Mature: 5.04

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
2.5 %Met     (Translated Protein)
2.8 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
2.3 %Met     (Mature Protein)
2.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTNLRKRLSRLYAEDVVDSLAARIEARVQQTQQRKLTRKDQWDEKDIVLITYGDQFKEES
CCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCEEEEEECHHHHHHH
KKTLKTFKKMYDSYLKSTFEVVHFLPFYPYSSDDGFSVIDYKAVNPELGDWEDIKEMEQS
HHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCEEEEEEECCCCCCCHHHHHHHHHH
ARLMFDFVCNHMSAKSDWFKRYLAGDEEFRNFFVEMDPKTDLSSVTRPRATPVLTPFQFD
HHHHHHHHHHHCCCHHHHHHHHHCCCHHHHHEEEEECCCCCHHHCCCCCCCCCCCCEECC
SGKVGHIWTTFSEDQIDLNFASPEVLYKMIDVLMFYLEEGAEYVRLDAVGFMWKVPGTSS
CCCCCEEEEECCCCCEEEECCCHHHHHHHHHHHHHHHHCCCCEEEEEEEEEEEECCCCCE
IHLEETHEIVKLFRDLVDIAAPGTIIITETNVPHVDNISYFGNGEKEAHMVYQFPLPPLV
EEHHHHHHHHHHHHHHHHHCCCCEEEEEECCCCCCCCCCCCCCCCCCEEEEEECCCCHHH
LHAIHHGNAEFLRNWAKNLELPEGKRTFFNFLASHDGIGLNPVRGIIPEAEILALVNDLE
HHHHHCCCHHHHHHHHHCCCCCCCHHHHHHHHHHCCCCCCCCHHHCCCHHHHHHHHHHHH
EEGALVSYKQNPDGSKSPYEINVTYMDALSKQADTDDLRMSRFLVAHAVLMSIPGVPAVY
HCCCEEEEECCCCCCCCCEEEEEEHHHHHCCCCCCHHHHHHHHHHHHHHHHHCCCCHHHH
VQSILGSRNDYSGVETTGHNRSINRKKYDLAEITAELEQRGSLRKATYDALTKLISTRKA
HHHHHCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHH
ESLFHPEIPMEVLESTAELFVVKRSSDVESIILIHNLSEKEVSYSLDSGVYTNLYTGSTV
HHHCCCCCCHHHHHCHHHEEEEECCCCCCEEEEEECCCHHHHHEECCCCCEEEEECCCEE
TGSDSIKLSGYEFCWLKTKNYREEQK
CCCCCEEECCEEEEEEECCCCCCCCC
>Mature Secondary Structure 
TNLRKRLSRLYAEDVVDSLAARIEARVQQTQQRKLTRKDQWDEKDIVLITYGDQFKEES
CHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCEEEEEECHHHHHHH
KKTLKTFKKMYDSYLKSTFEVVHFLPFYPYSSDDGFSVIDYKAVNPELGDWEDIKEMEQS
HHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCEEEEEEECCCCCCCHHHHHHHHHH
ARLMFDFVCNHMSAKSDWFKRYLAGDEEFRNFFVEMDPKTDLSSVTRPRATPVLTPFQFD
HHHHHHHHHHHCCCHHHHHHHHHCCCHHHHHEEEEECCCCCHHHCCCCCCCCCCCCEECC
SGKVGHIWTTFSEDQIDLNFASPEVLYKMIDVLMFYLEEGAEYVRLDAVGFMWKVPGTSS
CCCCCEEEEECCCCCEEEECCCHHHHHHHHHHHHHHHHCCCCEEEEEEEEEEEECCCCCE
IHLEETHEIVKLFRDLVDIAAPGTIIITETNVPHVDNISYFGNGEKEAHMVYQFPLPPLV
EEHHHHHHHHHHHHHHHHHCCCCEEEEEECCCCCCCCCCCCCCCCCCEEEEEECCCCHHH
LHAIHHGNAEFLRNWAKNLELPEGKRTFFNFLASHDGIGLNPVRGIIPEAEILALVNDLE
HHHHHCCCHHHHHHHHHCCCCCCCHHHHHHHHHHCCCCCCCCHHHCCCHHHHHHHHHHHH
EEGALVSYKQNPDGSKSPYEINVTYMDALSKQADTDDLRMSRFLVAHAVLMSIPGVPAVY
HCCCEEEEECCCCCCCCCEEEEEEHHHHHCCCCCCHHHHHHHHHHHHHHHHHCCCCHHHH
VQSILGSRNDYSGVETTGHNRSINRKKYDLAEITAELEQRGSLRKATYDALTKLISTRKA
HHHHHCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHH
ESLFHPEIPMEVLESTAELFVVKRSSDVESIILIHNLSEKEVSYSLDSGVYTNLYTGSTV
HHHCCCCCCHHHHHCHHHEEEEECCCCCCEEEEEECCCHHHHHEECCCCCEEEEECCCEE
TGSDSIKLSGYEFCWLKTKNYREEQK
CCCCCEEECCEEEEEEECCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 9097039; 9278503 [H]