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The map label for this gene is ycjT [H]

Identifier: 226225383

GI number: 226225383

Start: 2887329

End: 2889506

Strand: Reverse

Name: ycjT [H]

Synonym: Lm4b_02806

Alternate gene names: 226225383

Gene position: 2889506-2887329 (Counterclockwise)

Preceding gene: 226225384

Following gene: 226225382

Centisome position: 99.2

GC content: 40.22

Gene sequence:

>2178_bases
ATGAAGAAAATAATCGAGAAGGAATTTGCATTAAATTATCTAAATAAATATGGTAGCCAAATGACTGTGGGAAATGGCTA
TTTGGGCGTGCGCGGTGCTCTTGAAGAAGAATATCCGGAGCAAGTTCGCGGTATGTATGTGGCGGGGATTTATAATCGTC
CGAGCGGTTCGCTGAGTTCGGAACTTGTTAATTTGCCGGATGTGACGCGTTTCCAAGTGATGCTTGATGGGGAAAATTTT
TCGATGCAAGCGGGGAAAGTCCATGCTTACGAGCGTTATTTAGATATGAATACAGGAGAATTAATTCGAAAAATTACTTG
GGAAAGTAGCGCGGGCAAGAAGTATCAACTGAATTTTCACCGATTTGTTTCAAAAGCAGCAAAGCACGTCATTGCTGCTA
AAGTGGAGATAATGCCTTTGAGCGGTGCGGCGAAAGTCAAAGTAAAAACTGGTATTGATGCGCAACAAACTAATTTTGGA
ACACAGCAGCTGGCGGAGTTGAGTCTACGAATTTTTGATGAGAAGTTGATGGTTGGCGAATATGAGACCATCGAGAGCAA
ACAGCATATTACAATAGCAACAAAAGTGAATAAAAAAGGGATTTTCACTGCGAAAAACAGACAATTAATGACCGAAGTAG
AGCAAGAAATCGCTGAAAATCAACTATTTACTTTGGAGAAAATAAGCATGGTTAAAACATCCCTGGATCAAGTGGATGCG
GAACTTCCAAATACGACTTATGCAGAATTGAAAAGAGCTTCGGAAACTTTATGGGCAGATTTTTGGGAGCATGCGGGTGT
CCGTGTTGAGAGCACGAATGATTTTGACCAATTTGCACTAGATTTTGCGTGCTATCACTTAGAAATTATGACGCCGAAAG
ATGATTTTCGTTGTAGTGTTGGTGCGAAGGGACTTACCGGAGAAGGTTACAAGGGGCATGTTTTTTGGGATACAGAAATT
TTTATTATGCCATTTTTCCTTTATAATAAGCCGGAAATTGCCAAACAATTATTAAAATATCGCTACCTTCATTTGAAAGA
AGCGAAAGAAAAGGCAACGAAAAACGGCTATAATGGGGCGCTTTTTCCGTGGGAAAGTGCATTCTCTGGCGAAGAAGAAA
CGCCTGAATTTGCAGCGATTAATATTCGAACTGGCACACGTCAAAAAGTAGCGTCGGCGATTTCGGAGCATCATTTAGTT
GCGGATATTGCTTATGCGGTTTGTGAATATGAAACGGCGACTAAGGATGCTCAGTTTATGAAAGAGTGTGGTTCAGAGCT
GCTCCTTGAAACAGCGGAATTTTGGCTAAGCCGTGCAACTAATCGTAATGGACGACTGGAAATCCTTGATGTGATTGGTC
CGGACGAATACACCGAGCATATCGATAATAATACTTATACGAATTATATGGCACACTATAATGTAGAAAAAGCACTAGAC
TGGAATAAAGATAATGCCGATTTTGTAGCCCGTGCAGAAGCTTTTCTTGAAAAAATCTATTTACCTGTCGCAAATGAGTC
TGGCTTAATTCCGCAAGATGATACTTTCTTAGAAAAAGATTGGATAAATTTGGATAAATATAAGGCGGCACAAGGAACAC
AAGGGATTCTACTAGATTATTCTCGTCAAGAAGTGAACGAACTACAAATCTTAAAGCAAGCAGATTTAGTCATGTTGTTT
TACCTGTTTCCGAGGATGTTTGCGCCAGATGCTGTGAAGCAGAATTTGGATTACTATGAAAAACGAACAATTCATGATTC
TTCTTTAAGTAAAGCAATCCACGCAATTGTAGAAAATCGAACTGGTAATCGCGCACAAGCTTATCAATTTTTCCAAGAAG
CGTGTTTAATTGACCTTGGAAATGAGCCGCATTCATCGGATGACGGGCTCCATGCGGCCTCACTCGGCGCAATATGGTTG
GCGGTGGTTTTTGGATTTGCGGGTATAGATACTAGTGGAGAACTACTTGAAATCGCGCCAAACATACCGGATTCCTGGCA
ATCTGTGGCATTTGAATTCTCTTGGAAAGGTGAAAAAATCTTGGTAGAAATTGCGCAAAACAGATTACACCTTTCAAAAA
GCACAAAAAGCGTGCTAGAATTAGTTATTCACGGAGAAAAACATCAATTAACAGATACATTAACTATTAAGTTGGATCAG
AAGGATGTGTCTTTATGA

Upstream 100 bases:

>100_bases
CTGGAAAAGTGGAACTTGCTGGCGCTGAACAAGGTTATATCATACAAAAAATTGTTGAAAGTTTATACGAATCGGCAGAA
AAAGGTGAGAGTGTTTCTTT

Downstream 100 bases:

>100_bases
AAATTGTCATTGCACCGGATTCATTCAAAGAAAGTTTGACGGCGCATGAGGTGGCAGAGTATATTAAAGAGGGCTTTCAA
GAAATTTATCCGGATGCTGA

Product: maltose phosphorylase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 725; Mature: 725

Protein sequence:

>725_residues
MKKIIEKEFALNYLNKYGSQMTVGNGYLGVRGALEEEYPEQVRGMYVAGIYNRPSGSLSSELVNLPDVTRFQVMLDGENF
SMQAGKVHAYERYLDMNTGELIRKITWESSAGKKYQLNFHRFVSKAAKHVIAAKVEIMPLSGAAKVKVKTGIDAQQTNFG
TQQLAELSLRIFDEKLMVGEYETIESKQHITIATKVNKKGIFTAKNRQLMTEVEQEIAENQLFTLEKISMVKTSLDQVDA
ELPNTTYAELKRASETLWADFWEHAGVRVESTNDFDQFALDFACYHLEIMTPKDDFRCSVGAKGLTGEGYKGHVFWDTEI
FIMPFFLYNKPEIAKQLLKYRYLHLKEAKEKATKNGYNGALFPWESAFSGEEETPEFAAINIRTGTRQKVASAISEHHLV
ADIAYAVCEYETATKDAQFMKECGSELLLETAEFWLSRATNRNGRLEILDVIGPDEYTEHIDNNTYTNYMAHYNVEKALD
WNKDNADFVARAEAFLEKIYLPVANESGLIPQDDTFLEKDWINLDKYKAAQGTQGILLDYSRQEVNELQILKQADLVMLF
YLFPRMFAPDAVKQNLDYYEKRTIHDSSLSKAIHAIVENRTGNRAQAYQFFQEACLIDLGNEPHSSDDGLHAASLGAIWL
AVVFGFAGIDTSGELLEIAPNIPDSWQSVAFEFSWKGEKILVEIAQNRLHLSKSTKSVLELVIHGEKHQLTDTLTIKLDQ
KDVSL

Sequences:

>Translated_725_residues
MKKIIEKEFALNYLNKYGSQMTVGNGYLGVRGALEEEYPEQVRGMYVAGIYNRPSGSLSSELVNLPDVTRFQVMLDGENF
SMQAGKVHAYERYLDMNTGELIRKITWESSAGKKYQLNFHRFVSKAAKHVIAAKVEIMPLSGAAKVKVKTGIDAQQTNFG
TQQLAELSLRIFDEKLMVGEYETIESKQHITIATKVNKKGIFTAKNRQLMTEVEQEIAENQLFTLEKISMVKTSLDQVDA
ELPNTTYAELKRASETLWADFWEHAGVRVESTNDFDQFALDFACYHLEIMTPKDDFRCSVGAKGLTGEGYKGHVFWDTEI
FIMPFFLYNKPEIAKQLLKYRYLHLKEAKEKATKNGYNGALFPWESAFSGEEETPEFAAINIRTGTRQKVASAISEHHLV
ADIAYAVCEYETATKDAQFMKECGSELLLETAEFWLSRATNRNGRLEILDVIGPDEYTEHIDNNTYTNYMAHYNVEKALD
WNKDNADFVARAEAFLEKIYLPVANESGLIPQDDTFLEKDWINLDKYKAAQGTQGILLDYSRQEVNELQILKQADLVMLF
YLFPRMFAPDAVKQNLDYYEKRTIHDSSLSKAIHAIVENRTGNRAQAYQFFQEACLIDLGNEPHSSDDGLHAASLGAIWL
AVVFGFAGIDTSGELLEIAPNIPDSWQSVAFEFSWKGEKILVEIAQNRLHLSKSTKSVLELVIHGEKHQLTDTLTIKLDQ
KDVSL
>Mature_725_residues
MKKIIEKEFALNYLNKYGSQMTVGNGYLGVRGALEEEYPEQVRGMYVAGIYNRPSGSLSSELVNLPDVTRFQVMLDGENF
SMQAGKVHAYERYLDMNTGELIRKITWESSAGKKYQLNFHRFVSKAAKHVIAAKVEIMPLSGAAKVKVKTGIDAQQTNFG
TQQLAELSLRIFDEKLMVGEYETIESKQHITIATKVNKKGIFTAKNRQLMTEVEQEIAENQLFTLEKISMVKTSLDQVDA
ELPNTTYAELKRASETLWADFWEHAGVRVESTNDFDQFALDFACYHLEIMTPKDDFRCSVGAKGLTGEGYKGHVFWDTEI
FIMPFFLYNKPEIAKQLLKYRYLHLKEAKEKATKNGYNGALFPWESAFSGEEETPEFAAINIRTGTRQKVASAISEHHLV
ADIAYAVCEYETATKDAQFMKECGSELLLETAEFWLSRATNRNGRLEILDVIGPDEYTEHIDNNTYTNYMAHYNVEKALD
WNKDNADFVARAEAFLEKIYLPVANESGLIPQDDTFLEKDWINLDKYKAAQGTQGILLDYSRQEVNELQILKQADLVMLF
YLFPRMFAPDAVKQNLDYYEKRTIHDSSLSKAIHAIVENRTGNRAQAYQFFQEACLIDLGNEPHSSDDGLHAASLGAIWL
AVVFGFAGIDTSGELLEIAPNIPDSWQSVAFEFSWKGEKILVEIAQNRLHLSKSTKSVLELVIHGEKHQLTDTLTIKLDQ
KDVSL

Specific function: Unknown

COG id: COG1554

COG function: function code G; Trehalose and maltose hydrolases (possible phosphorylases)

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the glycosyl hydrolase 65 family [H]

Homologues:

Organism=Homo sapiens, GI187829418, Length=584, Percent_Identity=24.6575342465753, Blast_Score=132, Evalue=1e-30,
Organism=Escherichia coli, GI1787575, Length=719, Percent_Identity=48.8178025034771, Blast_Score=691, Evalue=0.0,
Organism=Saccharomyces cerevisiae, GI6325283, Length=442, Percent_Identity=22.8506787330317, Blast_Score=80, Evalue=2e-15,
Organism=Drosophila melanogaster, GI24583760, Length=165, Percent_Identity=34.5454545454545, Blast_Score=98, Evalue=2e-20,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR008928
- InterPro:   IPR012341
- InterPro:   IPR011013
- InterPro:   IPR005194
- InterPro:   IPR005195
- InterPro:   IPR005196
- InterPro:   IPR017045 [H]

Pfam domain/function: PF03633 Glyco_hydro_65C; PF03632 Glyco_hydro_65m; PF03636 Glyco_hydro_65N [H]

EC number: 3.2.1.- [C]

Molecular weight: Translated: 82265; Mature: 82265

Theoretical pI: Translated: 5.08; Mature: 5.08

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
2.2 %Met     (Translated Protein)
2.9 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
2.2 %Met     (Mature Protein)
2.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKKIIEKEFALNYLNKYGSQMTVGNGYLGVRGALEEEYPEQVRGMYVAGIYNRPSGSLSS
CCHHHHHHHHHHHHHHCCCEEEECCCCEECCCCHHHHHHHHHCCEEEEEEEECCCCCHHH
ELVNLPDVTRFQVMLDGENFSMQAGKVHAYERYLDMNTGELIRKITWESSAGKKYQLNFH
HHCCCCCCEEEEEEECCCCCEECCCCCHHHHHHHCCCHHHHHHHHHCCCCCCCEEEEHHH
RFVSKAAKHVIAAKVEIMPLSGAAKVKVKTGIDAQQTNFGTQQLAELSLRIFDEKLMVGE
HHHHHHHHHHHHEEEEEEECCCCEEEEEEECCCHHHCCCCHHHHHHHHHHHHHHHHCCCC
YETIESKQHITIATKVNKKGIFTAKNRQLMTEVEQEIAENQLFTLEKISMVKTSLDQVDA
HHHHCCCCEEEEEEEECCCCEEEECCHHHHHHHHHHHHHCCCEEHHHHHHHHHHHHHHHH
ELPNTTYAELKRASETLWADFWEHAGVRVESTNDFDQFALDFACYHLEIMTPKDDFRCSV
CCCCCHHHHHHHHHHHHHHHHHHHCCEEEECCCCHHHHHHHEEEEEEEEECCCCCCEEEC
GAKGLTGEGYKGHVFWDTEIFIMPFFLYNKPEIAKQLLKYRYLHLKEAKEKATKNGYNGA
CCCCCCCCCEEEEEEECCEEEEEEEECCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCC
LFPWESAFSGEEETPEFAAINIRTGTRQKVASAISEHHLVADIAYAVCEYETATKDAQFM
CCCCCCCCCCCCCCCCEEEEEECCCCHHHHHHHHHHHHHHHHHHHHHHHCHHCCHHHHHH
KECGSELLLETAEFWLSRATNRNGRLEILDVIGPDEYTEHIDNNTYTNYMAHYNVEKALD
HHHHHHHHHHHHHHHHHHHCCCCCCEEEEEECCCCHHHHHCCCCCEECEEEEECHHHHCC
WNKDNADFVARAEAFLEKIYLPVANESGLIPQDDTFLEKDWINLDKYKAAQGTQGILLDY
CCCCCHHHHHHHHHHHHHHCCEECCCCCCCCCCCCCHHHHCCCCHHHCCCCCCCCEEEEC
SRQEVNELQILKQADLVMLFYLFPRMFAPDAVKQNLDYYEKRTIHDSSLSKAIHAIVENR
CHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHCCHHHHHHCCCHHHHHHHHHHHHHCC
TGNRAQAYQFFQEACLIDLGNEPHSSDDGLHAASLGAIWLAVVFGFAGIDTSGELLEIAP
CCCHHHHHHHHHHHHEEECCCCCCCCCCCCHHHHHHHHHHHHHHHHCCCCCCCCEEEECC
NIPDSWQSVAFEFSWKGEKILVEIAQNRLHLSKSTKSVLELVIHGEKHQLTDTLTIKLDQ
CCCCCHHHEEEEEECCCCEEHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCEEEEEEEEC
KDVSL
CCCCC
>Mature Secondary Structure
MKKIIEKEFALNYLNKYGSQMTVGNGYLGVRGALEEEYPEQVRGMYVAGIYNRPSGSLSS
CCHHHHHHHHHHHHHHCCCEEEECCCCEECCCCHHHHHHHHHCCEEEEEEEECCCCCHHH
ELVNLPDVTRFQVMLDGENFSMQAGKVHAYERYLDMNTGELIRKITWESSAGKKYQLNFH
HHCCCCCCEEEEEEECCCCCEECCCCCHHHHHHHCCCHHHHHHHHHCCCCCCCEEEEHHH
RFVSKAAKHVIAAKVEIMPLSGAAKVKVKTGIDAQQTNFGTQQLAELSLRIFDEKLMVGE
HHHHHHHHHHHHEEEEEEECCCCEEEEEEECCCHHHCCCCHHHHHHHHHHHHHHHHCCCC
YETIESKQHITIATKVNKKGIFTAKNRQLMTEVEQEIAENQLFTLEKISMVKTSLDQVDA
HHHHCCCCEEEEEEEECCCCEEEECCHHHHHHHHHHHHHCCCEEHHHHHHHHHHHHHHHH
ELPNTTYAELKRASETLWADFWEHAGVRVESTNDFDQFALDFACYHLEIMTPKDDFRCSV
CCCCCHHHHHHHHHHHHHHHHHHHCCEEEECCCCHHHHHHHEEEEEEEEECCCCCCEEEC
GAKGLTGEGYKGHVFWDTEIFIMPFFLYNKPEIAKQLLKYRYLHLKEAKEKATKNGYNGA
CCCCCCCCCEEEEEEECCEEEEEEEECCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCC
LFPWESAFSGEEETPEFAAINIRTGTRQKVASAISEHHLVADIAYAVCEYETATKDAQFM
CCCCCCCCCCCCCCCCEEEEEECCCCHHHHHHHHHHHHHHHHHHHHHHHCHHCCHHHHHH
KECGSELLLETAEFWLSRATNRNGRLEILDVIGPDEYTEHIDNNTYTNYMAHYNVEKALD
HHHHHHHHHHHHHHHHHHHCCCCCCEEEEEECCCCHHHHHCCCCCEECEEEEECHHHHCC
WNKDNADFVARAEAFLEKIYLPVANESGLIPQDDTFLEKDWINLDKYKAAQGTQGILLDY
CCCCCHHHHHHHHHHHHHHCCEECCCCCCCCCCCCCHHHHCCCCHHHCCCCCCCCEEEEC
SRQEVNELQILKQADLVMLFYLFPRMFAPDAVKQNLDYYEKRTIHDSSLSKAIHAIVENR
CHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHCCHHHHHHCCCHHHHHHHHHHHHHCC
TGNRAQAYQFFQEACLIDLGNEPHSSDDGLHAASLGAIWLAVVFGFAGIDTSGELLEIAP
CCCHHHHHHHHHHHHEEECCCCCCCCCCCCHHHHHHHHHHHHHHHHCCCCCCCCEEEECC
NIPDSWQSVAFEFSWKGEKILVEIAQNRLHLSKSTKSVLELVIHGEKHQLTDTLTIKLDQ
CCCCCHHHEEEEEECCCCEEHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCEEEEEEEEC
KDVSL
CCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 9097039; 9278503 [H]