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The map label for this gene is emrD [C]

Identifier: 226225329

GI number: 226225329

Start: 2833954

End: 2835144

Strand: Direct

Name: emrD [C]

Synonym: Lm4b_02752

Alternate gene names: 226225329

Gene position: 2833954-2835144 (Clockwise)

Preceding gene: 226225322

Following gene: 226225362

Centisome position: 97.3

GC content: 37.7

Gene sequence:

>1191_bases
ATGGAAAAAAACATCGAAACAAAGAAAATAAATTTAGCATTACTTGTGTGCCTTGTGGGGTTTCCACAAATTAGCGAAAC
TATTTATACGCCTTCTTTAACGGAGATTGCTACGAGCTACGGCGTCTCATTAAATTTGGCTCAAATGACTTTAAGCATCT
ACTTTCTTGCTTTTGCAGTTGGTGTCTTCTTTTGGGGCGTTAGTTCCGATTTTTTAGGTCGACGAAAAGCAATGAACTTT
GGGATATTTATCTATATTATTGGTTGCTTCGTCTGCCTTTTTTCAAATAATATTACAATGCTTTTTATCGGACGCTTTGT
TCAAGCATTTGGCGCAAGTACGGGGTCTGTTACAACACAGACCATTTTGCGTGACAACTACCACGGTAATGATCGCCACC
ATTTATTTGCAAAAATCTCAGCCGCTTTGGCATTTTCACCGGCAATTGGGCCATTAATTGGTGGATTTATCGGACAATAT
TACGGCTTTCGCGTGGTTTTTTTATTTTTAGTAGTTATGGGAATGATATTGCTGTTCTGGAGTTTAAAACGACTACCAGA
AACAAAAACAGCGAACGCCACCCCATTCAGTGTGCAGAAAATGGTAGTAATTGGTAAAAAAATGATTTTTGACCGCTATA
CAATTGCTTTTGGAGTGCTAATTGGTATTTTTAATGGAATATTGTTTAGTTACTACTCTGAAGCTCCTACTATTTTTATT
GAGAGGTTTCATTTTAATCAGAGTCAGTATGGCTTCATGGGGTGCGCAGTGGCTACGGCAACTATTTTAGGAGCTTGGCT
ATCGAACTGGCGCTTAAAGCAGAATTCACCTCATAAAATCATTAAAGAAGGAATTTTGCTAGCTCTTATTGGCACTCTAT
TTCTTAGCATGGTTGCGCTGTTCCCGCTTACTATCCAAGTTATATTCTACATCTTCTTCATTTCTATTATCTTAGTAGGA
ATTGGCATGTCTTTACCAAATTGTTTAAGTCTAGCACTAGTGAAATTTCAAGAAGTTGCCGGTACAGCTGGCGCCTTTTT
AAGCTTAGGATATTATGTAATTGTCAGTCTTTGTACTTTCTTCATTGGCGTTTTGCATTCAGGATCAATACTTGTATTTC
CCGCATTCTGCCTAGTATTACTTCTAATTGCCTTGCTCTGTATAAAAATAGCTGCTAGAAATAACCCTTAG

Upstream 100 bases:

>100_bases
CTGCATGCTTTTTTACGAGCGTATTAAAATCATGGTCCCACCATTTCCCTGTGAAAGATGTTTTTTTCCCAGATAAATAA
CTTATTCAGGAGAGAATTTT

Downstream 100 bases:

>100_bases
CAAAAAACTGGCACCGTTTCTATCTTGAAACGGTGCCAGTTTTATTTTAAATGTTATCACAATATAAAATACTATTTATT
TCCCGGGGAATAAAAAAAGT

Product: efflux protein

Products: Proton [Cytoplasm]; multidrug [Periplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 396; Mature: 396

Protein sequence:

>396_residues
MEKNIETKKINLALLVCLVGFPQISETIYTPSLTEIATSYGVSLNLAQMTLSIYFLAFAVGVFFWGVSSDFLGRRKAMNF
GIFIYIIGCFVCLFSNNITMLFIGRFVQAFGASTGSVTTQTILRDNYHGNDRHHLFAKISAALAFSPAIGPLIGGFIGQY
YGFRVVFLFLVVMGMILLFWSLKRLPETKTANATPFSVQKMVVIGKKMIFDRYTIAFGVLIGIFNGILFSYYSEAPTIFI
ERFHFNQSQYGFMGCAVATATILGAWLSNWRLKQNSPHKIIKEGILLALIGTLFLSMVALFPLTIQVIFYIFFISIILVG
IGMSLPNCLSLALVKFQEVAGTAGAFLSLGYYVIVSLCTFFIGVLHSGSILVFPAFCLVLLLIALLCIKIAARNNP

Sequences:

>Translated_396_residues
MEKNIETKKINLALLVCLVGFPQISETIYTPSLTEIATSYGVSLNLAQMTLSIYFLAFAVGVFFWGVSSDFLGRRKAMNF
GIFIYIIGCFVCLFSNNITMLFIGRFVQAFGASTGSVTTQTILRDNYHGNDRHHLFAKISAALAFSPAIGPLIGGFIGQY
YGFRVVFLFLVVMGMILLFWSLKRLPETKTANATPFSVQKMVVIGKKMIFDRYTIAFGVLIGIFNGILFSYYSEAPTIFI
ERFHFNQSQYGFMGCAVATATILGAWLSNWRLKQNSPHKIIKEGILLALIGTLFLSMVALFPLTIQVIFYIFFISIILVG
IGMSLPNCLSLALVKFQEVAGTAGAFLSLGYYVIVSLCTFFIGVLHSGSILVFPAFCLVLLLIALLCIKIAARNNP
>Mature_396_residues
MEKNIETKKINLALLVCLVGFPQISETIYTPSLTEIATSYGVSLNLAQMTLSIYFLAFAVGVFFWGVSSDFLGRRKAMNF
GIFIYIIGCFVCLFSNNITMLFIGRFVQAFGASTGSVTTQTILRDNYHGNDRHHLFAKISAALAFSPAIGPLIGGFIGQY
YGFRVVFLFLVVMGMILLFWSLKRLPETKTANATPFSVQKMVVIGKKMIFDRYTIAFGVLIGIFNGILFSYYSEAPTIFI
ERFHFNQSQYGFMGCAVATATILGAWLSNWRLKQNSPHKIIKEGILLALIGTLFLSMVALFPLTIQVIFYIFFISIILVG
IGMSLPNCLSLALVKFQEVAGTAGAFLSLGYYVIVSLCTFFIGVLHSGSILVFPAFCLVLLLIALLCIKIAARNNP

Specific function: Multidrug Resistance Pump That Participates In A Low Energy Shock Adaptative Response. [C]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein (Potential) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the major facilitator superfamily. Bcr/CmlA family [H]

Homologues:

Organism=Escherichia coli, GI87082312, Length=348, Percent_Identity=24.1379310344828, Blast_Score=89, Evalue=4e-19,
Organism=Escherichia coli, GI1790794, Length=339, Percent_Identity=22.7138643067847, Blast_Score=85, Evalue=9e-18,
Organism=Escherichia coli, GI48994889, Length=172, Percent_Identity=29.6511627906977, Blast_Score=84, Evalue=2e-17,
Organism=Escherichia coli, GI1788509, Length=233, Percent_Identity=23.6051502145923, Blast_Score=79, Evalue=4e-16,
Organism=Escherichia coli, GI1790146, Length=369, Percent_Identity=21.9512195121951, Blast_Score=77, Evalue=1e-15,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004812
- InterPro:   IPR020846
- InterPro:   IPR011701
- InterPro:   IPR016196
- InterPro:   IPR005829 [H]

Pfam domain/function: PF07690 MFS_1 [H]

EC number: NA

Molecular weight: Translated: 43764; Mature: 43764

Theoretical pI: Translated: 9.64; Mature: 9.64

Prosite motif: PS50850 MFS

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.0 %Cys     (Translated Protein)
2.8 %Met     (Translated Protein)
4.8 %Cys+Met (Translated Protein)
2.0 %Cys     (Mature Protein)
2.8 %Met     (Mature Protein)
4.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure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HHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCC
>Mature Secondary Structure
MEKNIETKKINLALLVCLVGFPQISETIYTPSLTEIATSYGVSLNLAQMTLSIYFLAFAV
CCCCCCHHHHHHHHHHHHHCCCHHHHHHCCCCHHHHHHHHCCCHHHHHHHHHHHHHHHHH
GVFFWGVSSDFLGRRKAMNFGIFIYIIGCFVCLFSNNITMLFIGRFVQAFGASTGSVTTQ
HHHHHCCCHHHHHHHHHHCCHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHCCCCCCHHHH
TILRDNYHGNDRHHLFAKISAALAFSPAIGPLIGGFIGQYYGFRVVFLFLVVMGMILLFW
HHHHCCCCCCCHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
SLKRLPETKTANATPFSVQKMVVIGKKMIFDRYTIAFGVLIGIFNGILFSYYSEAPTIFI
HHHHCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEE
ERFHFNQSQYGFMGCAVATATILGAWLSNWRLKQNSPHKIIKEGILLALIGTLFLSMVAL
EEEECCCCCCCHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHH
FPLTIQVIFYIFFISIILVGIGMSLPNCLSLALVKFQEVAGTAGAFLSLGYYVIVSLCTF
HHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHCHHHHHHHHHHHHHHHHHHH
FIGVLHSGSILVFPAFCLVLLLIALLCIKIAARNNP
HHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: Proton [Periplasm]; multidrug [Cytoplasm] [C]

Specific reaction: Proton [Periplasm] + multidrug [Cytoplasm] = Proton [Cytoplasm] + multidrug [Periplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: NA