Click here to switch to the map view.

The map label for this gene is gshAB [H]

Identifier: 226225322

GI number: 226225322

Start: 2823146

End: 2825476

Strand: Direct

Name: gshAB [H]

Synonym: Lm4b_02745

Alternate gene names: 226225322

Gene position: 2823146-2825476 (Clockwise)

Preceding gene: 226225317

Following gene: 226225329

Centisome position: 96.93

GC content: 39.9

Gene sequence:

>2331_bases
ATGATAAAACTTGATATGACCATGCTTGATTCTTTTAAAGAAGACCCTAAGCTCCGGAAGCTTTTATTTTCTGGACATTT
TGGTTTAGAAAAAGAAAATATTCGCGTAACTTCTGATGGAAAATTGGCTCTTACGCCACATCCCGCTATTTTTGGTCCAA
AAGAGGATAACCCATATATTAAAACCGATTTTTCGGAAAGTCAGATTGAAATGATTACCCCGGTAACGGACTCGATTGAC
TCTGTATATGAATGGCTTGAAAATCTTCATAATATCGTTTCGTTGCGCTCAGAAAACGAACTACTTTGGCCGTCTAGTAA
TCCACCCATTTTACCAGCAGAAGAAGATATTCCAATTGCTGAATACAAAACGCCTGATAGCCCGGATAGAAAATACCGCG
AACATTTAGCAAAAGGCTACGGTAAAAAAATCCAATTATTATCTGGCATTCATTATAACTTCTCATTCCCAGAAGCTTTA
ATTGACGGGCTTTATGACAGAATTAGTCTTCCAGAAGAATCAAAGCAAGATTTTAAAAATCGGCTATATTTAAAAGTCGC
AAAATACTTTATGAAAAATCGCTGGTTGCTTATTTATTTAACTGGCGCAAGTCCGGTTTACCTTGCTGATTTCTCGAAAA
CGAAGCACGATGAATCCCTTCCGGACGGTAGTAGCGCGCTTCGTGATGGAATTTCTCTTCGAAATAGTAATGCTGGTTAT
AAAAACAAAGAAGCTCTTTACGTTGATTACAATTCTTTCGACGCTTATATTTCTAGCATCTCTAACTATATCGAAGCAGG
GAAAATCGAGAGTATGCGTGAATTTTACAACCCGATTCGTTTGAAAAATGCGCATACCGACCAAACAGTCGAAAGTTTAG
CGGAGCACGGCGTGGAATATTTAGAAATTCGCTCTATTGATCTGAATCCATTAGAGTCGAATGGTATTTCTAAAGACGAG
CTTGCTTTTATTCACCTATTTTTAATTAAAGGTTTGCTTTCAGAAGATCGCGAATTATGCTCCAACAACCAACAATTAGC
CGATGAAAATGAAAATAATATTGCGCTAAATGGTCTCGCGCAACCTGCCCTTAAAAATTGTGATAATGAAGAAATATCTG
TCGTAGAGGCCGGGCTTTTAGAGCTTAATAAGATGAGCGATTTCATCCAAAGCTTGCGACCAGAAGACACCAAACTCCAA
GCAATCATCGAAAAACAAAAAGAACGTCTGCTACACCCTGAAAAAACGATTGCTGCACAAGTGAAACAACAAGTAACAAA
AGAAGGCTACGTTGACTTCCATTTAAACCAAGCCAAAACTTACATGGAAGAAACCGAAGCTTTAGCCTACAAATTGATTG
GCGCAGAAGACATGGAGCTTTCCACCCAAATCATTTGGAAAGACGCCATCGCGCGCGGCATCAAAGTCGATGTATTAGAC
CGGGCCGAAAACTTCCTTCGCTTCCAAAAAGGCGACCATGTGGAGTATGTAAAACAAGCGAGCAAAACATCCAAAGACAA
CTACGTTTCCGTTTTAATGATGGAAAATAAAGTAGTTACTAAACTTGTACTTGCAGAACATGATATTCGCGTTCCATTTG
GCGACAGTTTTAGCGACCAAGCCCTTGCACTAGAAGCTTTCTCCCTATTTGAGGATAAACAAATTGTCGTTAAACCAAAA
TCCACCAACTACGGGTGGGGTATCAGTATTTTTAAAAATAAATTCACGCTGGAAGATTACCAAGAAGCTTTAAATATCGC
ATTTAGCTATGATAGCTCCGTTATTATCGAAGAGTTCATTCCTGGCGACGAATTCCGCTTCTTAGTTATTAACGACAAAG
TTGAAGCTGTACTAAAACGTGTCCCCGCTAACGTAACCGGCGACGGAATCCATACAGTGCGCGAGCTGGTTGAAGAAAAG
AACACTGATCCTTTGCGCGGAACAGACCATTTGAAACCACTTGAAAAAATCCGTACTGGTCCAGAAGAAACGCTCATGCT
CTCTATGCAAAACCTTTCTTGGGACAGCATACCTAAAGCAGAAGAGATTATCTACCTTCGCGAAAACTCCAACGTCAGCA
CAGGTGGCGACAGCATTGACTATACTGAAGAGATGGACGATTACTTCAAAGAGATAGCTATTCGTGCGACACAAGTACTT
GACGCCAAAATTTGCGGCGTAGACATAATTGTTCCACGTGAAACAATCGACCGTGATAAGCATGCTATCATCGAGCTTAA
TTTCAATCCCGCGATGCACATGCACTGCTTCCCTTATCAAGGGGAGAAGAAAAAAATTGGTGATAAGATTTTAGATTTCT
TATTTGAATAA

Upstream 100 bases:

>100_bases
AATTCGCTATAAAAAGTTACAAACCAATCATTATACTAATTTCACATATTGCGGTAAAATATCCCTATATGAACTTATTT
AAAACCCCTGAGGTGAAAAC

Downstream 100 bases:

>100_bases
AAAAATCCCAGCTACTAGTTTCGTAGCTGGGATTTTATATTACTCTCTCAATCCAATTTCACCATTTGAACTAATCACAT
CTTTATACCAATAATAGGAT

Product: bifunctional glutamate--cysteine ligase/glutathione synthetase

Products: NA

Alternate protein names: Gamma-GCS-GS; GCS-GS; Glutamate--cysteine ligase; Gamma-ECS; GCS; Gamma-glutamylcysteine synthetase; Glutathione synthetase; GSH synthetase; GS; GSH-S; GSHase; Glutathione synthase [H]

Number of amino acids: Translated: 776; Mature: 776

Protein sequence:

>776_residues
MIKLDMTMLDSFKEDPKLRKLLFSGHFGLEKENIRVTSDGKLALTPHPAIFGPKEDNPYIKTDFSESQIEMITPVTDSID
SVYEWLENLHNIVSLRSENELLWPSSNPPILPAEEDIPIAEYKTPDSPDRKYREHLAKGYGKKIQLLSGIHYNFSFPEAL
IDGLYDRISLPEESKQDFKNRLYLKVAKYFMKNRWLLIYLTGASPVYLADFSKTKHDESLPDGSSALRDGISLRNSNAGY
KNKEALYVDYNSFDAYISSISNYIEAGKIESMREFYNPIRLKNAHTDQTVESLAEHGVEYLEIRSIDLNPLESNGISKDE
LAFIHLFLIKGLLSEDRELCSNNQQLADENENNIALNGLAQPALKNCDNEEISVVEAGLLELNKMSDFIQSLRPEDTKLQ
AIIEKQKERLLHPEKTIAAQVKQQVTKEGYVDFHLNQAKTYMEETEALAYKLIGAEDMELSTQIIWKDAIARGIKVDVLD
RAENFLRFQKGDHVEYVKQASKTSKDNYVSVLMMENKVVTKLVLAEHDIRVPFGDSFSDQALALEAFSLFEDKQIVVKPK
STNYGWGISIFKNKFTLEDYQEALNIAFSYDSSVIIEEFIPGDEFRFLVINDKVEAVLKRVPANVTGDGIHTVRELVEEK
NTDPLRGTDHLKPLEKIRTGPEETLMLSMQNLSWDSIPKAEEIIYLRENSNVSTGGDSIDYTEEMDDYFKEIAIRATQVL
DAKICGVDIIVPRETIDRDKHAIIELNFNPAMHMHCFPYQGEKKKIGDKILDFLFE

Sequences:

>Translated_776_residues
MIKLDMTMLDSFKEDPKLRKLLFSGHFGLEKENIRVTSDGKLALTPHPAIFGPKEDNPYIKTDFSESQIEMITPVTDSID
SVYEWLENLHNIVSLRSENELLWPSSNPPILPAEEDIPIAEYKTPDSPDRKYREHLAKGYGKKIQLLSGIHYNFSFPEAL
IDGLYDRISLPEESKQDFKNRLYLKVAKYFMKNRWLLIYLTGASPVYLADFSKTKHDESLPDGSSALRDGISLRNSNAGY
KNKEALYVDYNSFDAYISSISNYIEAGKIESMREFYNPIRLKNAHTDQTVESLAEHGVEYLEIRSIDLNPLESNGISKDE
LAFIHLFLIKGLLSEDRELCSNNQQLADENENNIALNGLAQPALKNCDNEEISVVEAGLLELNKMSDFIQSLRPEDTKLQ
AIIEKQKERLLHPEKTIAAQVKQQVTKEGYVDFHLNQAKTYMEETEALAYKLIGAEDMELSTQIIWKDAIARGIKVDVLD
RAENFLRFQKGDHVEYVKQASKTSKDNYVSVLMMENKVVTKLVLAEHDIRVPFGDSFSDQALALEAFSLFEDKQIVVKPK
STNYGWGISIFKNKFTLEDYQEALNIAFSYDSSVIIEEFIPGDEFRFLVINDKVEAVLKRVPANVTGDGIHTVRELVEEK
NTDPLRGTDHLKPLEKIRTGPEETLMLSMQNLSWDSIPKAEEIIYLRENSNVSTGGDSIDYTEEMDDYFKEIAIRATQVL
DAKICGVDIIVPRETIDRDKHAIIELNFNPAMHMHCFPYQGEKKKIGDKILDFLFE
>Mature_776_residues
MIKLDMTMLDSFKEDPKLRKLLFSGHFGLEKENIRVTSDGKLALTPHPAIFGPKEDNPYIKTDFSESQIEMITPVTDSID
SVYEWLENLHNIVSLRSENELLWPSSNPPILPAEEDIPIAEYKTPDSPDRKYREHLAKGYGKKIQLLSGIHYNFSFPEAL
IDGLYDRISLPEESKQDFKNRLYLKVAKYFMKNRWLLIYLTGASPVYLADFSKTKHDESLPDGSSALRDGISLRNSNAGY
KNKEALYVDYNSFDAYISSISNYIEAGKIESMREFYNPIRLKNAHTDQTVESLAEHGVEYLEIRSIDLNPLESNGISKDE
LAFIHLFLIKGLLSEDRELCSNNQQLADENENNIALNGLAQPALKNCDNEEISVVEAGLLELNKMSDFIQSLRPEDTKLQ
AIIEKQKERLLHPEKTIAAQVKQQVTKEGYVDFHLNQAKTYMEETEALAYKLIGAEDMELSTQIIWKDAIARGIKVDVLD
RAENFLRFQKGDHVEYVKQASKTSKDNYVSVLMMENKVVTKLVLAEHDIRVPFGDSFSDQALALEAFSLFEDKQIVVKPK
STNYGWGISIFKNKFTLEDYQEALNIAFSYDSSVIIEEFIPGDEFRFLVINDKVEAVLKRVPANVTGDGIHTVRELVEEK
NTDPLRGTDHLKPLEKIRTGPEETLMLSMQNLSWDSIPKAEEIIYLRENSNVSTGGDSIDYTEEMDDYFKEIAIRATQVL
DAKICGVDIIVPRETIDRDKHAIIELNFNPAMHMHCFPYQGEKKKIGDKILDFLFE

Specific function: Synthesizes glutathione from L-glutamate and L-cysteine via gamma-L-glutamyl-L-cysteine [H]

COG id: COG2918

COG function: function code H; Gamma-glutamylcysteine synthetase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 ATP-grasp domain [H]

Homologues:

Organism=Escherichia coli, GI1789044, Length=443, Percent_Identity=28.4424379232506, Blast_Score=176, Evalue=7e-45,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR011761
- InterPro:   IPR013815
- InterPro:   IPR013816
- InterPro:   IPR007370
- InterPro:   IPR006335
- InterPro:   IPR020561 [H]

Pfam domain/function: PF01071 GARS_A; PF04262 Glu_cys_ligase [H]

EC number: =6.3.2.2; =6.3.2.3 [H]

Molecular weight: Translated: 88613; Mature: 88613

Theoretical pI: Translated: 4.71; Mature: 4.71

Prosite motif: PS50975 ATP_GRASP

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
2.1 %Met     (Translated Protein)
2.6 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
2.1 %Met     (Mature Protein)
2.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MIKLDMTMLDSFKEDPKLRKLLFSGHFGLEKENIRVTSDGKLALTPHPAIFGPKEDNPYI
CEEECHHHHHHHCCCHHHHHHHHHCCCCCCCCCEEEECCCEEEECCCCCEECCCCCCCEE
KTDFSESQIEMITPVTDSIDSVYEWLENLHNIVSLRSENELLWPSSNPPILPAEEDIPIA
EECCCCCCEEEECCCHHHHHHHHHHHHHHHHHHHHCCCCCEECCCCCCCCCCCCCCCCCC
EYKTPDSPDRKYREHLAKGYGKKIQLLSGIHYNFSFPEALIDGLYDRISLPEESKQDFKN
CCCCCCCCHHHHHHHHHHCCCCEEEEEECCCCCCCCHHHHHHHHHHHCCCCCHHHHHHHH
RLYLKVAKYFMKNRWLLIYLTGASPVYLADFSKTKHDESLPDGSSALRDGISLRNSNAGY
HHHHHHHHHHHHCCEEEEEECCCCEEEEECCCCCCCCCCCCCCHHHHHHCCCCCCCCCCC
KNKEALYVDYNSFDAYISSISNYIEAGKIESMREFYNPIRLKNAHTDQTVESLAEHGVEY
CCCCEEEEECCHHHHHHHHHHHHHHHCCHHHHHHHHCCEEECCCCCHHHHHHHHHCCCEE
LEIRSIDLNPLESNGISKDELAFIHLFLIKGLLSEDRELCSNNQQLADENENNIALNGLA
EEEEECCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCEEECCCC
QPALKNCDNEEISVVEAGLLELNKMSDFIQSLRPEDTKLQAIIEKQKERLLHPEKTIAAQ
CHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHCCCCHHHHHHH
VKQQVTKEGYVDFHLNQAKTYMEETEALAYKLIGAEDMELSTQIIWKDAIARGIKVDVLD
HHHHHHHCCCEEEEEHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHCCCEEEEHH
RAENFLRFQKGDHVEYVKQASKTSKDNYVSVLMMENKVVTKLVLAEHDIRVPFGDSFSDQ
HHHHHHHHCCCCHHHHHHHHHCCCCCCEEEEEEECCHHHHHHHHHHCCEECCCCCCCCCC
ALALEAFSLFEDKQIVVKPKSTNYGWGISIFKNKFTLEDYQEALNIAFSYDSSVIIEEFI
HHHHHHHHHCCCCEEEEECCCCCCCCEEEEEECCCCHHHHHHHHHEEEECCCCCHHHHCC
PGDEFRFLVINDKVEAVLKRVPANVTGDGIHTVRELVEEKNTDPLRGTDHLKPLEKIRTG
CCCCEEEEEECCHHHHHHHHCCCCCCCCHHHHHHHHHHHCCCCCCCCCCCCCHHHHHHCC
PEETLMLSMQNLSWDSIPKAEEIIYLRENSNVSTGGDSIDYTEEMDDYFKEIAIRATQVL
CCCEEEEEECCCCCCCCCCCCCEEEEECCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHH
DAKICGVDIIVPRETIDRDKHAIIELNFNPAMHMHCFPYQGEKKKIGDKILDFLFE
HHHHCCEEEEECHHHCCCCCEEEEEEECCCCEEEEEECCCCCHHHHHHHHHHHHCC
>Mature Secondary Structure
MIKLDMTMLDSFKEDPKLRKLLFSGHFGLEKENIRVTSDGKLALTPHPAIFGPKEDNPYI
CEEECHHHHHHHCCCHHHHHHHHHCCCCCCCCCEEEECCCEEEECCCCCEECCCCCCCEE
KTDFSESQIEMITPVTDSIDSVYEWLENLHNIVSLRSENELLWPSSNPPILPAEEDIPIA
EECCCCCCEEEECCCHHHHHHHHHHHHHHHHHHHHCCCCCEECCCCCCCCCCCCCCCCCC
EYKTPDSPDRKYREHLAKGYGKKIQLLSGIHYNFSFPEALIDGLYDRISLPEESKQDFKN
CCCCCCCCHHHHHHHHHHCCCCEEEEEECCCCCCCCHHHHHHHHHHHCCCCCHHHHHHHH
RLYLKVAKYFMKNRWLLIYLTGASPVYLADFSKTKHDESLPDGSSALRDGISLRNSNAGY
HHHHHHHHHHHHCCEEEEEECCCCEEEEECCCCCCCCCCCCCCHHHHHHCCCCCCCCCCC
KNKEALYVDYNSFDAYISSISNYIEAGKIESMREFYNPIRLKNAHTDQTVESLAEHGVEY
CCCCEEEEECCHHHHHHHHHHHHHHHCCHHHHHHHHCCEEECCCCCHHHHHHHHHCCCEE
LEIRSIDLNPLESNGISKDELAFIHLFLIKGLLSEDRELCSNNQQLADENENNIALNGLA
EEEEECCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCEEECCCC
QPALKNCDNEEISVVEAGLLELNKMSDFIQSLRPEDTKLQAIIEKQKERLLHPEKTIAAQ
CHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHCCCCHHHHHHH
VKQQVTKEGYVDFHLNQAKTYMEETEALAYKLIGAEDMELSTQIIWKDAIARGIKVDVLD
HHHHHHHCCCEEEEEHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHCCCEEEEHH
RAENFLRFQKGDHVEYVKQASKTSKDNYVSVLMMENKVVTKLVLAEHDIRVPFGDSFSDQ
HHHHHHHHCCCCHHHHHHHHHCCCCCCEEEEEEECCHHHHHHHHHHCCEECCCCCCCCCC
ALALEAFSLFEDKQIVVKPKSTNYGWGISIFKNKFTLEDYQEALNIAFSYDSSVIIEEFI
HHHHHHHHHCCCCEEEEECCCCCCCCEEEEEECCCCHHHHHHHHHEEEECCCCCHHHHCC
PGDEFRFLVINDKVEAVLKRVPANVTGDGIHTVRELVEEKNTDPLRGTDHLKPLEKIRTG
CCCCEEEEEECCHHHHHHHHCCCCCCCCHHHHHHHHHHHCCCCCCCCCCCCCHHHHHHCC
PEETLMLSMQNLSWDSIPKAEEIIYLRENSNVSTGGDSIDYTEEMDDYFKEIAIRATQVL
CCCEEEEEECCCCCCCCCCCCCEEEEECCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHH
DAKICGVDIIVPRETIDRDKHAIIELNFNPAMHMHCFPYQGEKKKIGDKILDFLFE
HHHHCCEEEEECHHHCCCCCEEEEEEECCCCEEEEEECCCCCHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA