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The map label for this gene is ycjM [H]

Identifier: 226225286

GI number: 226225286

Start: 2780100

End: 2781782

Strand: Direct

Name: ycjM [H]

Synonym: Lm4b_02709b

Alternate gene names: 226225286

Gene position: 2780100-2781782 (Clockwise)

Preceding gene: 226225285

Following gene: 226225287

Centisome position: 95.45

GC content: 40.88

Gene sequence:

>1683_bases
ATGCAAACCGAACTGGTTAATCAAATAGAAGTAAAACTAAGAAAAATTTATCAAGCTGCATACCAACCCGCATACTTGGA
AAAAATGCTCGCATGCGCCGAAAACTATTCTAATAATGCGCGTGGTTCGATTGATACTATTTCCGAAAAAAACGTCTATC
TTATTGCCTATGGCGACAGCATTTTCGAAAAAAACAAGCATCCGTTACAAACATTGAATGAATTCCTACAAGAATATGCG
CAAGATGTTATTACAGATGTCCACTTGCTGCCGATTTTCCCTAGTACGTCAGATGATGGCTTCTCTGTCACAGACTACAA
GCAAATTGACGAACAGCTTGGCGACTGGGATGACGTTCAAAAAATGTCTGAGAACTTCCGAGTGATGCTTGATTTCGTAG
CCAATCATATGTCGAAATCAAGTGATTGGTTTAAGCGATTCTCCGATAACGAAGCGCCGTACAATCAATTTTTCATCGAG
AAGGACAGCCAATTTGATTATAAAAACGTCACACGTCCGCGGACTTCTCCGCTGTTCCACAAATACGAAAACGGTAAAGA
ACTTTGGACTACTTTTAGCGAAGATCAGTTAGACTTAAATGTCCGTAATATCGATTGCCTTGTAGCCTTAACAGATGTAC
TGCTTTTCTATGCATCCAAACAAGCGACAAGCATTCGACTTGATGCCATTGGATTTTTATGGAAAACTTCTGGCACTACT
TGTATGCACCTGCCAGAAACCCATGAGATCATCACGCTTTGGCGTCTGCTGATTGATGAACTATATCCAAATCTGCAAAT
TATCACAGAAACAAACGTCCCTCATGAAGAAAACATTAGTTATTTTGGCGATGGTGAAAATGAAGCAAATATGGTTTATC
AGTTCCCGCTTCCGCCACTTGTGCTCCATACCTTCACTTGCCATGATACAACGAAACTTTCGAAGTGGGCGAAATCCATT
TCACAAGTTTCTAGCACAGCGACTTATTTTAACTTCTTAGCAAGTCATGACGGAATTGGTATGCGCCCTGCGACCGGAAT
TCTTTCCGACGAAGAAATCAATTCTCTCGTCCAAAAAGCGGTCCAAAACGGTGGCCAAGTTTCCTATAAAGACAACGCAG
ATGGCACGCAGTCGGTATACGAACTTAATATTAACTACGGTGAAGCCCTGCAAAACCCGGGCGAAGACACAACTGAAGAA
CTCGTAACGAAAAAAATTATCGCTGCTCATAGTATTTTGCTAACACTTCAAGGCGTCCCAGCGATTTACTATCATTCCCT
TCTAGGTTCTAAAAACGACCTTGTTGGTTACGAAGAATCCGGTATTAATCGCCGCATCAATCGTGAAAAACTGGAAAAAA
ATCAATTGGTTCATGAACTTGAAACAGACACTTATCGCCAAACCATTTTCACATCGCTGAAAAAATTGGTGCAAATCAGA
CGAAATCACACCGCTTTTTCACCATTTGCAACCCAAGAAATTTTAGATTTAGGACCAGATGTTTTTGCGATAAAACGGGA
ATCTGAGGAAGAATGTATTTACGGAATTATCAACGTCACTTCTCATAATATCCGCAAAACGCTCGCTTTCTCTGGCACGA
ATCTATTAACCAATCAACCCGTTACGAGTGAACTTGATTTAACTGCGTATGAAGTTGTCTGGATTAAGAAGGCGGTCCAA
TGA

Upstream 100 bases:

>100_bases
GAAGTTTCACTAGCGGAGGAAAAAATCGCAGAACTAAAAGAAAACACTATCTGTATTCCTAAAAAAGGATTTGCAACTAT
TTTGTTGGAAGGAGAATAAT

Downstream 100 bases:

>100_bases
AAATCGTCATCGCACCTGATTCATTCAAAGAGAGCGCCACTGCTGTTGAAGTAGCAAATGCCATAAAAAAAGGCTGGACT
AAAGCACGTCCAGCCGATCA

Product: Sucrose phosphorylase

Products: NA

Alternate protein names: Sucrose glucosyltransferase [H]

Number of amino acids: Translated: 560; Mature: 560

Protein sequence:

>560_residues
MQTELVNQIEVKLRKIYQAAYQPAYLEKMLACAENYSNNARGSIDTISEKNVYLIAYGDSIFEKNKHPLQTLNEFLQEYA
QDVITDVHLLPIFPSTSDDGFSVTDYKQIDEQLGDWDDVQKMSENFRVMLDFVANHMSKSSDWFKRFSDNEAPYNQFFIE
KDSQFDYKNVTRPRTSPLFHKYENGKELWTTFSEDQLDLNVRNIDCLVALTDVLLFYASKQATSIRLDAIGFLWKTSGTT
CMHLPETHEIITLWRLLIDELYPNLQIITETNVPHEENISYFGDGENEANMVYQFPLPPLVLHTFTCHDTTKLSKWAKSI
SQVSSTATYFNFLASHDGIGMRPATGILSDEEINSLVQKAVQNGGQVSYKDNADGTQSVYELNINYGEALQNPGEDTTEE
LVTKKIIAAHSILLTLQGVPAIYYHSLLGSKNDLVGYEESGINRRINREKLEKNQLVHELETDTYRQTIFTSLKKLVQIR
RNHTAFSPFATQEILDLGPDVFAIKRESEEECIYGIINVTSHNIRKTLAFSGTNLLTNQPVTSELDLTAYEVVWIKKAVQ

Sequences:

>Translated_560_residues
MQTELVNQIEVKLRKIYQAAYQPAYLEKMLACAENYSNNARGSIDTISEKNVYLIAYGDSIFEKNKHPLQTLNEFLQEYA
QDVITDVHLLPIFPSTSDDGFSVTDYKQIDEQLGDWDDVQKMSENFRVMLDFVANHMSKSSDWFKRFSDNEAPYNQFFIE
KDSQFDYKNVTRPRTSPLFHKYENGKELWTTFSEDQLDLNVRNIDCLVALTDVLLFYASKQATSIRLDAIGFLWKTSGTT
CMHLPETHEIITLWRLLIDELYPNLQIITETNVPHEENISYFGDGENEANMVYQFPLPPLVLHTFTCHDTTKLSKWAKSI
SQVSSTATYFNFLASHDGIGMRPATGILSDEEINSLVQKAVQNGGQVSYKDNADGTQSVYELNINYGEALQNPGEDTTEE
LVTKKIIAAHSILLTLQGVPAIYYHSLLGSKNDLVGYEESGINRRINREKLEKNQLVHELETDTYRQTIFTSLKKLVQIR
RNHTAFSPFATQEILDLGPDVFAIKRESEEECIYGIINVTSHNIRKTLAFSGTNLLTNQPVTSELDLTAYEVVWIKKAVQ
>Mature_560_residues
MQTELVNQIEVKLRKIYQAAYQPAYLEKMLACAENYSNNARGSIDTISEKNVYLIAYGDSIFEKNKHPLQTLNEFLQEYA
QDVITDVHLLPIFPSTSDDGFSVTDYKQIDEQLGDWDDVQKMSENFRVMLDFVANHMSKSSDWFKRFSDNEAPYNQFFIE
KDSQFDYKNVTRPRTSPLFHKYENGKELWTTFSEDQLDLNVRNIDCLVALTDVLLFYASKQATSIRLDAIGFLWKTSGTT
CMHLPETHEIITLWRLLIDELYPNLQIITETNVPHEENISYFGDGENEANMVYQFPLPPLVLHTFTCHDTTKLSKWAKSI
SQVSSTATYFNFLASHDGIGMRPATGILSDEEINSLVQKAVQNGGQVSYKDNADGTQSVYELNINYGEALQNPGEDTTEE
LVTKKIIAAHSILLTLQGVPAIYYHSLLGSKNDLVGYEESGINRRINREKLEKNQLVHELETDTYRQTIFTSLKKLVQIR
RNHTAFSPFATQEILDLGPDVFAIKRESEEECIYGIINVTSHNIRKTLAFSGTNLLTNQPVTSELDLTAYEVVWIKKAVQ

Specific function: Unknown

COG id: COG0366

COG function: function code G; Glycosidases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the sucrose phosphorylase family [H]

Homologues:

Organism=Escherichia coli, GI87081873, Length=556, Percent_Identity=42.2661870503597, Blast_Score=451, Evalue=1e-128,
Organism=Escherichia coli, GI1790687, Length=177, Percent_Identity=28.8135593220339, Blast_Score=74, Evalue=3e-14,
Organism=Drosophila melanogaster, GI24583747, Length=229, Percent_Identity=31.0043668122271, Blast_Score=89, Evalue=1e-17,
Organism=Drosophila melanogaster, GI24583749, Length=229, Percent_Identity=31.0043668122271, Blast_Score=89, Evalue=1e-17,
Organism=Drosophila melanogaster, GI45549022, Length=367, Percent_Identity=23.9782016348774, Blast_Score=85, Evalue=1e-16,
Organism=Drosophila melanogaster, GI24583745, Length=227, Percent_Identity=28.6343612334802, Blast_Score=79, Evalue=6e-15,
Organism=Drosophila melanogaster, GI24586589, Length=250, Percent_Identity=28, Blast_Score=77, Evalue=3e-14,
Organism=Drosophila melanogaster, GI24586591, Length=171, Percent_Identity=28.0701754385965, Blast_Score=67, Evalue=3e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR006047
- InterPro:   IPR017853
- InterPro:   IPR013781
- InterPro:   IPR016377 [H]

Pfam domain/function: PF00128 Alpha-amylase [H]

EC number: =2.4.1.7 [H]

Molecular weight: Translated: 63989; Mature: 63989

Theoretical pI: Translated: 4.74; Mature: 4.74

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
1.4 %Met     (Translated Protein)
2.3 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
1.4 %Met     (Mature Protein)
2.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MQTELVNQIEVKLRKIYQAAYQPAYLEKMLACAENYSNNARGSIDTISEKNVYLIAYGDS
CCCHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHCCCCCCCCCCEEECCCCEEEEEECCH
IFEKNKHPLQTLNEFLQEYAQDVITDVHLLPIFPSTSDDGFSVTDYKQIDEQLGDWDDVQ
HHHCCCCHHHHHHHHHHHHHHHHHCCEEEEEECCCCCCCCCCCCHHHHHHHHCCCHHHHH
KMSENFRVMLDFVANHMSKSSDWFKRFSDNEAPYNQFFIEKDSQFDYKNVTRPRTSPLFH
HHHHHHHHHHHHHHHHHCCCHHHHHHHCCCCCCCHHEEEECCCCCCHHCCCCCCCCCHHH
KYENGKELWTTFSEDQLDLNVRNIDCLVALTDVLLFYASKQATSIRLDAIGFLWKTSGTT
HHCCCHHHHHCCCCCCEEEEECCHHHHHHHHHHHHHHHCCCCCEEEEEEEEEEEECCCCE
CMHLPETHEIITLWRLLIDELYPNLQIITETNVPHEENISYFGDGENEANMVYQFPLPPL
EEECCCHHHHHHHHHHHHHHHCCCEEEEEECCCCCCCCCCCCCCCCCCCCEEEECCCCHH
VLHTFTCHDTTKLSKWAKSISQVSSTATYFNFLASHDGIGMRPATGILSDEEINSLVQKA
HHEEEECCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCHHHHHHHHHHH
VQNGGQVSYKDNADGTQSVYELNINYGEALQNPGEDTTEELVTKKIIAAHSILLTLQGVP
HHCCCCEEECCCCCCCCEEEEEECCHHHHHCCCCCCHHHHHHHHHHHHHHHHHHEECCCC
AIYYHSLLGSKNDLVGYEESGINRRINREKLEKNQLVHELETDTYRQTIFTSLKKLVQIR
HHHHHHHHCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
RNHTAFSPFATQEILDLGPDVFAIKRESEEECIYGIINVTSHNIRKTLAFSGTNLLTNQP
HCCCCCCCHHHHHHHHCCCCEEEEECCCCCCCEEEEEECCCCHHHHHHEECCCCCCCCCC
VTSELDLTAYEVVWIKKAVQ
CCCCCCCHHHHHHHHHHHCC
>Mature Secondary Structure
MQTELVNQIEVKLRKIYQAAYQPAYLEKMLACAENYSNNARGSIDTISEKNVYLIAYGDS
CCCHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHCCCCCCCCCCEEECCCCEEEEEECCH
IFEKNKHPLQTLNEFLQEYAQDVITDVHLLPIFPSTSDDGFSVTDYKQIDEQLGDWDDVQ
HHHCCCCHHHHHHHHHHHHHHHHHCCEEEEEECCCCCCCCCCCCHHHHHHHHCCCHHHHH
KMSENFRVMLDFVANHMSKSSDWFKRFSDNEAPYNQFFIEKDSQFDYKNVTRPRTSPLFH
HHHHHHHHHHHHHHHHHCCCHHHHHHHCCCCCCCHHEEEECCCCCCHHCCCCCCCCCHHH
KYENGKELWTTFSEDQLDLNVRNIDCLVALTDVLLFYASKQATSIRLDAIGFLWKTSGTT
HHCCCHHHHHCCCCCCEEEEECCHHHHHHHHHHHHHHHCCCCCEEEEEEEEEEEECCCCE
CMHLPETHEIITLWRLLIDELYPNLQIITETNVPHEENISYFGDGENEANMVYQFPLPPL
EEECCCHHHHHHHHHHHHHHHCCCEEEEEECCCCCCCCCCCCCCCCCCCCEEEECCCCHH
VLHTFTCHDTTKLSKWAKSISQVSSTATYFNFLASHDGIGMRPATGILSDEEINSLVQKA
HHEEEECCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCHHHHHHHHHHH
VQNGGQVSYKDNADGTQSVYELNINYGEALQNPGEDTTEELVTKKIIAAHSILLTLQGVP
HHCCCCEEECCCCCCCCEEEEEECCHHHHHCCCCCCHHHHHHHHHHHHHHHHHHEECCCC
AIYYHSLLGSKNDLVGYEESGINRRINREKLEKNQLVHELETDTYRQTIFTSLKKLVQIR
HHHHHHHHCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
RNHTAFSPFATQEILDLGPDVFAIKRESEEECIYGIINVTSHNIRKTLAFSGTNLLTNQP
HCCCCCCCHHHHHHHHCCCCEEEEECCCCCCCEEEEEECCCCHHHHHHEECCCCCCCCCC
VTSELDLTAYEVVWIKKAVQ
CCCCCCCHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 9097039; 9278503 [H]