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The map label for this gene is ami

Identifier: 226225105

GI number: 226225105

Start: 2606915

End: 2609227

Strand: Direct

Name: ami

Synonym: Lm4b_02526

Alternate gene names: 226225105

Gene position: 2606915-2609227 (Clockwise)

Preceding gene: 226225066

Following gene: 226225111

Centisome position: 89.5

GC content: 40.12

Gene sequence:

>2313_bases
TTGAAAAAATTAGTAAAATCGGCGGTTGTTTTTGCAAGCCTTGTTTTTATTGGCACCTCCGCTACTATGATTACAGAAAA
AGCAAGTGCTGCTTCCATTGATCCGGTGCAAAAAGCAGATGGTCAAGCTACTTATATCCCCAAAGGAGTTAGAGATGGGA
CTGCAACGGAAGAACATGACGGCTTTGAAGATGGAACTAATAGCGTACTACAGCAAGTCCCTTTGCTTCGCGCAACAACA
GGATACCCTGATGTTAATGCCTATATTAAATCAAACAAATTTTCAACAGCAAAAATAGAAAAACAATTAAAAAGCCAATT
TCCTAAATTCAACTATCGTAATGGTTACGGCAAACCAGAAGGAATAGTTATTCATGAAACAGCAAATAATTCATCTACTA
TTACAGGTGAAATCAATTATATGAGTACCAACTACAACAATGCCTTTGTTCACGCTTTTGTAGACAAGTCTCGTATAATC
CAAATCCATCCTACTGAAAATGGTGTTTGGGGAGCAGGACAATATGCTAATGCTCGTTTTATCCAAGTTGAATTAGTTCG
TTCGAAGACATTCGATGAATTTGCTCGTTCTATTAATAACTATGCCTATTATGCTGCGTACCTTTTAGATCAATATAACC
TTCCTGTTGATAGCGCGCACAGTGACGGCAAAGGAACAGTCTGGTCCCACGATGCAGTTACACGTTATTTAGGCGGAACA
ACCCATACTGATCCTGTTTCTTATTTCAACCAATGGGGTTATAATTTCAATAGTTTTGTTACATTGATTAATGAAAAGTA
CAAAGCGATTCAAGCTAGCAAAGTTACTTACGACAAAATCGAATACGATAAAGGCGTCACAGCCTACGCTAGAGTTAAAA
CTGCACCTGGTAATGCAGTTTGGACAAAACCTTACAGAACAGAAGGCTCTAAACTTGTTAATCAACTTTCTGTCTACCAA
GGTAAAAACATGCGGATCTTGCGTGAAGCTAAAACACCAATTACTACTTGGTATCAATTCAGCATTGACGGGAAAGTAAT
CGGTTGGGTTGATACTCGTGCACTCGATACGTTCTACAAACAAAGCATGGAACAACCAGCTAATTTAACTCGCTATGTCG
CTTCCAACAAAACTGGTGAGGCCTACTACAAAGTACCTGTTGTTGATGCGGATGTCAAATGGGGCACTTTAGCTGCTTAT
AAAGATCAAAAACTAACCGTGGATAAACAAGCAACGGTGGAAGGGCAACTTTGGTATCGCGTTAGAACAAGCACTACCTT
TATTGGTTGGACGAAAGCTTCCAACTTAACAGCTACCTCTCCATTTGATAAGATTGAATACGATAAAGGTGTCACAGCCT
ATGCTAGAGTTAAAACTGCACCTGGTAATGCCGTTTGGACAAAACCTTACAGAACGGAAGGCTCTAAACTTGTCAATCAA
CTTTCGGTCTACCAAGGCAAAAACATGCGGATCTTGCGCGAAGCTAAAACAGTAATTACTACCTGGTATCAATTTAGTAT
TGATGGGAAAGTAATCGGTTGGGTTGATACTCGTGCACTCGATACGTTCTATAAGCAAAGCATGGAGAAAGATACTAATT
TAACTCGCTATGTGATTGCGAACAAAGTAAACGAAGCGTACTACAAAGTGCCTGTTGTGGATGCGGATGTTAGATGGGGC
ACTTTAGCTGCTTATAAAGATCAAAAACTAACCGTGGATAAACAAGCAACGGTCGAAGGACAGCTTTGGTACCGCGTTAG
AACAAGCACTACTTTTATCGGTTGGACAAAAGCTTCGAACTTAACAACAACTACGCCATACGATAAAATTGAATATGATA
AAGGCGCCACAGCCTATGCTAGAGTAAAAACTGCGCCAGGCAATGCCGTTTGGACAAAACCTTACAGAACGGAAGGCTCT
AAACTTGTTAATCAACTTTCGGTCTACCAAGGTAAAAACATGCGAATCTTGCGCGAAGCTAAAACAGTAATTACTACCTG
GTATCAATTCAGCATTGACGGGAAAGTAATCGGTTGGGTTGATACTCGTGCACTCGATACGTTCTATAAGCAAAGCATGG
AGAAAGATACTAATTTAACTCGCTATGTGATTGCGAACAAAGTAAACGAAGCGTACTACAAAGTTCCTGTTGTAGATGCG
GATGTTAGATGGGGCACTTTAGCTGCTTACAAAGGTCAAAAACTAACCGTGGATAAACAAGCAACGGTCGAAGGGCAACT
TTGGTATCGAATAAGAACAAGCTCCACCTTTATTGGTTGGACAAAAGCTTCGAACCTTAGTGCTACTAAATAA

Upstream 100 bases:

>100_bases
ACCAATATCCATTGACTACAACCAGGCGTGTTGTCATAATTAGAAATAGAATACTTTTATTTAATATAAAAAGTAGTGCA
GTTAGGAGAGGATTTAAACT

Downstream 100 bases:

>100_bases
TAATAATAAAAATCTCCCAGCCAATTATGGCTGGGAGATTTTTATTATTACCTTATTTATTATTAAGTGCAGCGCCAACA
AAATCTTTAAACAAGCTTTG

Product: autolysin, amidase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 770; Mature: 770

Protein sequence:

>770_residues
MKKLVKSAVVFASLVFIGTSATMITEKASAASIDPVQKADGQATYIPKGVRDGTATEEHDGFEDGTNSVLQQVPLLRATT
GYPDVNAYIKSNKFSTAKIEKQLKSQFPKFNYRNGYGKPEGIVIHETANNSSTITGEINYMSTNYNNAFVHAFVDKSRII
QIHPTENGVWGAGQYANARFIQVELVRSKTFDEFARSINNYAYYAAYLLDQYNLPVDSAHSDGKGTVWSHDAVTRYLGGT
THTDPVSYFNQWGYNFNSFVTLINEKYKAIQASKVTYDKIEYDKGVTAYARVKTAPGNAVWTKPYRTEGSKLVNQLSVYQ
GKNMRILREAKTPITTWYQFSIDGKVIGWVDTRALDTFYKQSMEQPANLTRYVASNKTGEAYYKVPVVDADVKWGTLAAY
KDQKLTVDKQATVEGQLWYRVRTSTTFIGWTKASNLTATSPFDKIEYDKGVTAYARVKTAPGNAVWTKPYRTEGSKLVNQ
LSVYQGKNMRILREAKTVITTWYQFSIDGKVIGWVDTRALDTFYKQSMEKDTNLTRYVIANKVNEAYYKVPVVDADVRWG
TLAAYKDQKLTVDKQATVEGQLWYRVRTSTTFIGWTKASNLTTTTPYDKIEYDKGATAYARVKTAPGNAVWTKPYRTEGS
KLVNQLSVYQGKNMRILREAKTVITTWYQFSIDGKVIGWVDTRALDTFYKQSMEKDTNLTRYVIANKVNEAYYKVPVVDA
DVRWGTLAAYKGQKLTVDKQATVEGQLWYRIRTSSTFIGWTKASNLSATK

Sequences:

>Translated_770_residues
MKKLVKSAVVFASLVFIGTSATMITEKASAASIDPVQKADGQATYIPKGVRDGTATEEHDGFEDGTNSVLQQVPLLRATT
GYPDVNAYIKSNKFSTAKIEKQLKSQFPKFNYRNGYGKPEGIVIHETANNSSTITGEINYMSTNYNNAFVHAFVDKSRII
QIHPTENGVWGAGQYANARFIQVELVRSKTFDEFARSINNYAYYAAYLLDQYNLPVDSAHSDGKGTVWSHDAVTRYLGGT
THTDPVSYFNQWGYNFNSFVTLINEKYKAIQASKVTYDKIEYDKGVTAYARVKTAPGNAVWTKPYRTEGSKLVNQLSVYQ
GKNMRILREAKTPITTWYQFSIDGKVIGWVDTRALDTFYKQSMEQPANLTRYVASNKTGEAYYKVPVVDADVKWGTLAAY
KDQKLTVDKQATVEGQLWYRVRTSTTFIGWTKASNLTATSPFDKIEYDKGVTAYARVKTAPGNAVWTKPYRTEGSKLVNQ
LSVYQGKNMRILREAKTVITTWYQFSIDGKVIGWVDTRALDTFYKQSMEKDTNLTRYVIANKVNEAYYKVPVVDADVRWG
TLAAYKDQKLTVDKQATVEGQLWYRVRTSTTFIGWTKASNLTTTTPYDKIEYDKGATAYARVKTAPGNAVWTKPYRTEGS
KLVNQLSVYQGKNMRILREAKTVITTWYQFSIDGKVIGWVDTRALDTFYKQSMEKDTNLTRYVIANKVNEAYYKVPVVDA
DVRWGTLAAYKGQKLTVDKQATVEGQLWYRIRTSSTFIGWTKASNLSATK
>Mature_770_residues
MKKLVKSAVVFASLVFIGTSATMITEKASAASIDPVQKADGQATYIPKGVRDGTATEEHDGFEDGTNSVLQQVPLLRATT
GYPDVNAYIKSNKFSTAKIEKQLKSQFPKFNYRNGYGKPEGIVIHETANNSSTITGEINYMSTNYNNAFVHAFVDKSRII
QIHPTENGVWGAGQYANARFIQVELVRSKTFDEFARSINNYAYYAAYLLDQYNLPVDSAHSDGKGTVWSHDAVTRYLGGT
THTDPVSYFNQWGYNFNSFVTLINEKYKAIQASKVTYDKIEYDKGVTAYARVKTAPGNAVWTKPYRTEGSKLVNQLSVYQ
GKNMRILREAKTPITTWYQFSIDGKVIGWVDTRALDTFYKQSMEQPANLTRYVASNKTGEAYYKVPVVDADVKWGTLAAY
KDQKLTVDKQATVEGQLWYRVRTSTTFIGWTKASNLTATSPFDKIEYDKGVTAYARVKTAPGNAVWTKPYRTEGSKLVNQ
LSVYQGKNMRILREAKTVITTWYQFSIDGKVIGWVDTRALDTFYKQSMEKDTNLTRYVIANKVNEAYYKVPVVDADVRWG
TLAAYKDQKLTVDKQATVEGQLWYRVRTSTTFIGWTKASNLTTTTPYDKIEYDKGATAYARVKTAPGNAVWTKPYRTEGS
KLVNQLSVYQGKNMRILREAKTVITTWYQFSIDGKVIGWVDTRALDTFYKQSMEKDTNLTRYVIANKVNEAYYKVPVVDA
DVRWGTLAAYKGQKLTVDKQATVEGQLWYRIRTSSTFIGWTKASNLSATK

Specific function: Mediates the entry of Listeria monocytogenes into cells [H]

COG id: COG5632

COG function: function code M; N-acetylmuramoyl-L-alanine amidase

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Contains 7 LRR (leucine-rich) repeats [H]

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR014755
- InterPro:   IPR014756
- InterPro:   IPR001611
- InterPro:   IPR013378
- InterPro:   IPR012569 [H]

Pfam domain/function: PF09479 Flg_new; PF00560 LRR_1; PF08191 LRR_adjacent [H]

EC number: NA

Molecular weight: Translated: 86996; Mature: 86996

Theoretical pI: Translated: 9.95; Mature: 9.95

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
1.2 %Met     (Translated Protein)
1.2 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
1.2 %Met     (Mature Protein)
1.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKKLVKSAVVFASLVFIGTSATMITEKASAASIDPVQKADGQATYIPKGVRDGTATEEHD
CHHHHHHHHHHHHHHHHCCCCEEEEECCCCCCCCCHHHCCCCEEECCCCCCCCCCCCCCC
GFEDGTNSVLQQVPLLRATTGYPDVNAYIKSNKFSTAKIEKQLKSQFPKFNYRNGYGKPE
CCCCHHHHHHHHCCCEEEECCCCCCEEEEECCCCCHHHHHHHHHHHCCCCCCCCCCCCCC
GIVIHETANNSSTITGEINYMSTNYNNAFVHAFVDKSRIIQIHPTENGVWGAGQYANARF
CEEEEEECCCCCEEEEEEEEEEECCCCEEEEEEECCCEEEEEECCCCCCCCCCCCCCEEE
IQVELVRSKTFDEFARSINNYAYYAAYLLDQYNLPVDSAHSDGKGTVWSHDAVTRYLGGT
EEEEEECCCCHHHHHHHHCCCEEEEEEEHHHCCCCCCCCCCCCCCCEECHHHHHHHCCCC
THTDPVSYFNQWGYNFNSFVTLINEKYKAIQASKVTYDKIEYDKGVTAYARVKTAPGNAV
CCCCHHHHHHHCCCCHHHHHHHHHHHHHHHHHCCCEEHHEECCCCCEEEEEEEECCCCCE
WTKPYRTEGSKLVNQLSVYQGKNMRILREAKTPITTWYQFSIDGKVIGWVDTRALDTFYK
ECCCCCCCHHHHHHHHHHHCCCCEEEEEECCCCEEEEEEEEECCEEEEEECHHHHHHHHH
QSMEQPANLTRYVASNKTGEAYYKVPVVDADVKWGTLAAYKDQKLTVDKQATVEGQLWYR
HHHCCCCHHHHHHHCCCCCCEEEEEEEEECCCCCCEEEEECCCEEEECCCCEECCEEEEE
VRTSTTFIGWTKASNLTATSPFDKIEYDKGVTAYARVKTAPGNAVWTKPYRTEGSKLVNQ
EECCEEEEEEECCCCCEECCCCCCCCCCCCCEEEEEEEECCCCCEECCCCCCCHHHHHHH
LSVYQGKNMRILREAKTVITTWYQFSIDGKVIGWVDTRALDTFYKQSMEKDTNLTRYVIA
HHHHCCCCCEEEHHHHEEEEEEEEEEECCEEEEEECHHHHHHHHHHHHHCCCCCEEEEEE
NKVNEAYYKVPVVDADVRWGTLAAYKDQKLTVDKQATVEGQLWYRVRTSTTFIGWTKASN
CCCCCCEEEEEEEECCCCCCEEEEECCCEEEECCCCEECCEEEEEEECCEEEEEEEECCC
LTTTTPYDKIEYDKGATAYARVKTAPGNAVWTKPYRTEGSKLVNQLSVYQGKNMRILREA
CEECCCCCCEECCCCCCEEEEEEECCCCCEECCCCCCCHHHHHHHHHHHCCCCCEEEHHH
KTVITTWYQFSIDGKVIGWVDTRALDTFYKQSMEKDTNLTRYVIANKVNEAYYKVPVVDA
HEEEEEEEEEEECCEEEEEECHHHHHHHHHHHHHCCCCCEEEEEECCCCCCEEEEEEEEC
DVRWGTLAAYKGQKLTVDKQATVEGQLWYRIRTSSTFIGWTKASNLSATK
CCCCCEEEEECCCEEEECCCCEECCEEEEEEECCCEEEEEEECCCCCCCC
>Mature Secondary Structure
MKKLVKSAVVFASLVFIGTSATMITEKASAASIDPVQKADGQATYIPKGVRDGTATEEHD
CHHHHHHHHHHHHHHHHCCCCEEEEECCCCCCCCCHHHCCCCEEECCCCCCCCCCCCCCC
GFEDGTNSVLQQVPLLRATTGYPDVNAYIKSNKFSTAKIEKQLKSQFPKFNYRNGYGKPE
CCCCHHHHHHHHCCCEEEECCCCCCEEEEECCCCCHHHHHHHHHHHCCCCCCCCCCCCCC
GIVIHETANNSSTITGEINYMSTNYNNAFVHAFVDKSRIIQIHPTENGVWGAGQYANARF
CEEEEEECCCCCEEEEEEEEEEECCCCEEEEEEECCCEEEEEECCCCCCCCCCCCCCEEE
IQVELVRSKTFDEFARSINNYAYYAAYLLDQYNLPVDSAHSDGKGTVWSHDAVTRYLGGT
EEEEEECCCCHHHHHHHHCCCEEEEEEEHHHCCCCCCCCCCCCCCCEECHHHHHHHCCCC
THTDPVSYFNQWGYNFNSFVTLINEKYKAIQASKVTYDKIEYDKGVTAYARVKTAPGNAV
CCCCHHHHHHHCCCCHHHHHHHHHHHHHHHHHCCCEEHHEECCCCCEEEEEEEECCCCCE
WTKPYRTEGSKLVNQLSVYQGKNMRILREAKTPITTWYQFSIDGKVIGWVDTRALDTFYK
ECCCCCCCHHHHHHHHHHHCCCCEEEEEECCCCEEEEEEEEECCEEEEEECHHHHHHHHH
QSMEQPANLTRYVASNKTGEAYYKVPVVDADVKWGTLAAYKDQKLTVDKQATVEGQLWYR
HHHCCCCHHHHHHHCCCCCCEEEEEEEEECCCCCCEEEEECCCEEEECCCCEECCEEEEE
VRTSTTFIGWTKASNLTATSPFDKIEYDKGVTAYARVKTAPGNAVWTKPYRTEGSKLVNQ
EECCEEEEEEECCCCCEECCCCCCCCCCCCCEEEEEEEECCCCCEECCCCCCCHHHHHHH
LSVYQGKNMRILREAKTVITTWYQFSIDGKVIGWVDTRALDTFYKQSMEKDTNLTRYVIA
HHHHCCCCCEEEHHHHEEEEEEEEEEECCEEEEEECHHHHHHHHHHHHHCCCCCEEEEEE
NKVNEAYYKVPVVDADVRWGTLAAYKDQKLTVDKQATVEGQLWYRVRTSTTFIGWTKASN
CCCCCCEEEEEEEECCCCCCEEEEECCCEEEECCCCEECCEEEEEEECCEEEEEEEECCC
LTTTTPYDKIEYDKGATAYARVKTAPGNAVWTKPYRTEGSKLVNQLSVYQGKNMRILREA
CEECCCCCCEECCCCCCEEEEEEECCCCCEECCCCCCCHHHHHHHHHHHCCCCCEEEHHH
KTVITTWYQFSIDGKVIGWVDTRALDTFYKQSMEKDTNLTRYVIANKVNEAYYKVPVVDA
HEEEEEEEEEEECCEEEEEECHHHHHHHHHHHHHCCCCCEEEEEECCCCCCEEEEEEEEC
DVRWGTLAAYKGQKLTVDKQATVEGQLWYRIRTSSTFIGWTKASNLSATK
CCCCCEEEEECCCEEEECCCCEECCEEEEEEECCCEEEEEEECCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 1905979; 11679669; 10635330 [H]