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The map label for this gene is bbmA [H]

Identifier: 226224733

GI number: 226224733

Start: 2209638

End: 2211413

Strand: Reverse

Name: bbmA [H]

Synonym: Lm4b_02150

Alternate gene names: 226224733

Gene position: 2211413-2209638 (Counterclockwise)

Preceding gene: 226224735

Following gene: 226224732

Centisome position: 75.92

GC content: 38.34

Gene sequence:

>1776_bases
ATGGAAAAAGCAGGGATTTATCATCAACCAGCGAGTAGCTATGCTTATAGTTATGACGCTAAAACACTACATATTCGGAT
TAGAACGAAACGCTTGGATATTAGCGAGGTTACTCTAATTGCTGCCGACCCTTACTTATGGAAAGATGGAAAATGGCAAA
GTGAATCTTATACAATGCGCAAAATCGCAGAAACAGAAGAGCACGATTATTGGTTTTTTGCAATTACACCAGAACATAGA
CGTCTTCAATATGGCTTTTTACTAACAGATACAGAAGGAGAAACAACATTTTACGGCGGACGTGGTTTCTTTGAGCCAAC
TGAGGCTAATTTAGCTACGATGGATTATTACTTTAAATTTCCATTTATCCATGCGGTGGATACTTTTGAGGCGCCTGAAT
GGGTTGGGAATACTATTTGGTACCAGATTTTCCCAGAACGTTTTGCAAATGGGAATCCGGCTATTTCTCCGGAAAATGCC
TTGCCGTGGGGAAGTAAAGATCCGAACACAACAGACTTTTTCGGCGGGGATATAGAAGGAATTATCGAGCATTTAGATTA
TTTAGCAGAGCTTGGTATTAATGGGGTTTATTTGACACCTGTGTTTGAAGCACCAACCAATCATAAATACGATACGGTTG
ATTATAAAAAAATTGATCCACATTTTGGAGATAAAGAAACGTTTAGAAAATTAGTTCAGGAAGCGCATAAACGTGGGATT
CGCATTATGTTAGATGCGGTGTTCAATCACATTGGCGATACTTCAGCAGAGTGGCAAGATGTTGTCTTAAATGAAGAAAA
ATCTGCATATCGTGATTGGTTCCACATCCATAGTTTCCCTGTTCGTCAAAATGAAAATGGCAATATTGAAGGAGAACCAA
CACTTTCTTACGATACATTTGCTTTCACGACGCATATGCCAAAATTAAATACGGCTAACCCAGAAGTTCAGAAGTATCTA
CTGGATATTGCAACTTACTGGATTCGCGAATTTGACATTGATGGCTGGCGCTTAGATGTTGCTAATGAAGTAGATCATGC
TTTCTGGAAAGAATTTAAAAAGGCTGTCCAAGCGGAAAAAGAAGATATTTATATTCTTGGTGAAATTTGGCATGATTCGT
GGATTTGGTTACTTGGAGATGAATTCCACGCCGTAATGAACTATCCATTTACACAAACGATTATTGAGAACTTTATTGAA
GAAAAAATCACACCGGAGCAAATGGTTTCTGGTATTAATGAACAATATATGCGCTACCCAAATCAAGTGAATGAAGTAAT
GTTTAATATGCTTGATAGCCATGATACGGCGCGTATTTTAACTCGTGCTAATAATGATGAGGATAAAGTGAAACAAGCGC
TTGCCTTCATGTTCGCGCACACTGGTTCGCCGTGTATTTATTACGGAACTGAAATCGGCATGGACGGAGGAAATGACCCA
GGTTGCCGTAAGTGTATGGAATGGGATGAAACGAAGCAAAACCAAGATATGCTAGCGTTTACGAAACAATTAATTGCCCT
AAGAAAAGATAACCAAGCAATTATCACATCCGGCGAATTAACTTGGCTAGGTGCAAGTAGCGAAACGGGCATTACAGCAT
TTTCTAAAGAATTAAATGGTGAAAAACTGCATTTCCTTTTCAATCAAGCAAAAGAAGATAAAGATTTCACAATTTCATTT
GAAAATACAGCGACAGATATTTGGAACAACCGAGCTGTAACAGAAAATCTTGTTGTCCCAGCAAAAGGATTTCTTGTTAT
TAAAGAAAACAGCTAA

Upstream 100 bases:

>100_bases
AGAAAATCTTGTTACCGTTAACGAGCGAGAAAAGGTTGCAAACGCTTTAATGGAAATGCTAATTTGAAATAGAACGATAG
GTGATATAGGAGGAGAAAAA

Downstream 100 bases:

>100_bases
ATCAATGGCGTAAAGGAATTTTTCACATGTTTACCGAAAAAATGTTGTTATCGGTAACATGTGAAATCTCCTTGTAAACG
CTTTCGAAATCAGCATATAA

Product: maltogenic amylase

Products: alpha-D-glucose; beta-D-glucose; amylose(n-1) [C]

Alternate protein names: NA

Number of amino acids: Translated: 591; Mature: 591

Protein sequence:

>591_residues
MEKAGIYHQPASSYAYSYDAKTLHIRIRTKRLDISEVTLIAADPYLWKDGKWQSESYTMRKIAETEEHDYWFFAITPEHR
RLQYGFLLTDTEGETTFYGGRGFFEPTEANLATMDYYFKFPFIHAVDTFEAPEWVGNTIWYQIFPERFANGNPAISPENA
LPWGSKDPNTTDFFGGDIEGIIEHLDYLAELGINGVYLTPVFEAPTNHKYDTVDYKKIDPHFGDKETFRKLVQEAHKRGI
RIMLDAVFNHIGDTSAEWQDVVLNEEKSAYRDWFHIHSFPVRQNENGNIEGEPTLSYDTFAFTTHMPKLNTANPEVQKYL
LDIATYWIREFDIDGWRLDVANEVDHAFWKEFKKAVQAEKEDIYILGEIWHDSWIWLLGDEFHAVMNYPFTQTIIENFIE
EKITPEQMVSGINEQYMRYPNQVNEVMFNMLDSHDTARILTRANNDEDKVKQALAFMFAHTGSPCIYYGTEIGMDGGNDP
GCRKCMEWDETKQNQDMLAFTKQLIALRKDNQAIITSGELTWLGASSETGITAFSKELNGEKLHFLFNQAKEDKDFTISF
ENTATDIWNNRAVTENLVVPAKGFLVIKENS

Sequences:

>Translated_591_residues
MEKAGIYHQPASSYAYSYDAKTLHIRIRTKRLDISEVTLIAADPYLWKDGKWQSESYTMRKIAETEEHDYWFFAITPEHR
RLQYGFLLTDTEGETTFYGGRGFFEPTEANLATMDYYFKFPFIHAVDTFEAPEWVGNTIWYQIFPERFANGNPAISPENA
LPWGSKDPNTTDFFGGDIEGIIEHLDYLAELGINGVYLTPVFEAPTNHKYDTVDYKKIDPHFGDKETFRKLVQEAHKRGI
RIMLDAVFNHIGDTSAEWQDVVLNEEKSAYRDWFHIHSFPVRQNENGNIEGEPTLSYDTFAFTTHMPKLNTANPEVQKYL
LDIATYWIREFDIDGWRLDVANEVDHAFWKEFKKAVQAEKEDIYILGEIWHDSWIWLLGDEFHAVMNYPFTQTIIENFIE
EKITPEQMVSGINEQYMRYPNQVNEVMFNMLDSHDTARILTRANNDEDKVKQALAFMFAHTGSPCIYYGTEIGMDGGNDP
GCRKCMEWDETKQNQDMLAFTKQLIALRKDNQAIITSGELTWLGASSETGITAFSKELNGEKLHFLFNQAKEDKDFTISF
ENTATDIWNNRAVTENLVVPAKGFLVIKENS
>Mature_591_residues
MEKAGIYHQPASSYAYSYDAKTLHIRIRTKRLDISEVTLIAADPYLWKDGKWQSESYTMRKIAETEEHDYWFFAITPEHR
RLQYGFLLTDTEGETTFYGGRGFFEPTEANLATMDYYFKFPFIHAVDTFEAPEWVGNTIWYQIFPERFANGNPAISPENA
LPWGSKDPNTTDFFGGDIEGIIEHLDYLAELGINGVYLTPVFEAPTNHKYDTVDYKKIDPHFGDKETFRKLVQEAHKRGI
RIMLDAVFNHIGDTSAEWQDVVLNEEKSAYRDWFHIHSFPVRQNENGNIEGEPTLSYDTFAFTTHMPKLNTANPEVQKYL
LDIATYWIREFDIDGWRLDVANEVDHAFWKEFKKAVQAEKEDIYILGEIWHDSWIWLLGDEFHAVMNYPFTQTIIENFIE
EKITPEQMVSGINEQYMRYPNQVNEVMFNMLDSHDTARILTRANNDEDKVKQALAFMFAHTGSPCIYYGTEIGMDGGNDP
GCRKCMEWDETKQNQDMLAFTKQLIALRKDNQAIITSGELTWLGASSETGITAFSKELNGEKLHFLFNQAKEDKDFTISF
ENTATDIWNNRAVTENLVVPAKGFLVIKENS

Specific function: Hydrolyzes beta-cyclodextrin to maltose and glucose, soluble starch to maltose and glucose, and pullulan to panose with trace amounts of maltose and glucose. It is also able to hydrolyze acarbose. Can also exhibit a transglycosylation activity transferrin

COG id: COG0366

COG function: function code G; Glycosidases

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the glycosyl hydrolase 13 family. BbmA subfamily [H]

Homologues:

Organism=Homo sapiens, GI187423904, Length=405, Percent_Identity=25.1851851851852, Blast_Score=92, Evalue=1e-18,
Organism=Homo sapiens, GI61744479, Length=117, Percent_Identity=36.7521367521368, Blast_Score=71, Evalue=3e-12,
Organism=Homo sapiens, GI61744475, Length=117, Percent_Identity=36.7521367521368, Blast_Score=71, Evalue=3e-12,
Organism=Homo sapiens, GI61744477, Length=117, Percent_Identity=36.7521367521368, Blast_Score=71, Evalue=4e-12,
Organism=Homo sapiens, GI65506891, Length=117, Percent_Identity=36.7521367521368, Blast_Score=71, Evalue=4e-12,
Organism=Homo sapiens, GI61744481, Length=117, Percent_Identity=36.7521367521368, Blast_Score=70, Evalue=4e-12,
Organism=Homo sapiens, GI61744483, Length=117, Percent_Identity=36.7521367521368, Blast_Score=70, Evalue=5e-12,
Organism=Escherichia coli, GI1786604, Length=473, Percent_Identity=31.2896405919662, Blast_Score=223, Evalue=3e-59,
Organism=Escherichia coli, GI1790687, Length=389, Percent_Identity=27.7634961439589, Blast_Score=114, Evalue=2e-26,
Organism=Escherichia coli, GI1789995, Length=145, Percent_Identity=31.7241379310345, Blast_Score=78, Evalue=1e-15,
Organism=Saccharomyces cerevisiae, GI6322245, Length=229, Percent_Identity=30.5676855895196, Blast_Score=100, Evalue=1e-21,
Organism=Saccharomyces cerevisiae, GI6321726, Length=406, Percent_Identity=23.3990147783251, Blast_Score=90, Evalue=1e-18,
Organism=Saccharomyces cerevisiae, GI6321731, Length=403, Percent_Identity=21.8362282878412, Blast_Score=89, Evalue=3e-18,
Organism=Saccharomyces cerevisiae, GI6319776, Length=403, Percent_Identity=21.8362282878412, Blast_Score=88, Evalue=4e-18,
Organism=Saccharomyces cerevisiae, GI6322241, Length=406, Percent_Identity=22.9064039408867, Blast_Score=84, Evalue=6e-17,
Organism=Saccharomyces cerevisiae, GI6322021, Length=406, Percent_Identity=22.9064039408867, Blast_Score=84, Evalue=6e-17,
Organism=Saccharomyces cerevisiae, GI6324416, Length=406, Percent_Identity=22.9064039408867, Blast_Score=84, Evalue=7e-17,
Organism=Drosophila melanogaster, GI24586587, Length=463, Percent_Identity=24.8380129589633, Blast_Score=97, Evalue=3e-20,
Organism=Drosophila melanogaster, GI24583747, Length=233, Percent_Identity=28.755364806867, Blast_Score=93, Evalue=4e-19,
Organism=Drosophila melanogaster, GI24583749, Length=233, Percent_Identity=28.755364806867, Blast_Score=93, Evalue=4e-19,
Organism=Drosophila melanogaster, GI221330053, Length=182, Percent_Identity=30.7692307692308, Blast_Score=87, Evalue=4e-17,
Organism=Drosophila melanogaster, GI24586591, Length=241, Percent_Identity=28.6307053941909, Blast_Score=86, Evalue=7e-17,
Organism=Drosophila melanogaster, GI24586589, Length=183, Percent_Identity=32.2404371584699, Blast_Score=84, Evalue=2e-16,
Organism=Drosophila melanogaster, GI24586599, Length=497, Percent_Identity=23.3400402414487, Blast_Score=84, Evalue=2e-16,
Organism=Drosophila melanogaster, GI45549022, Length=229, Percent_Identity=27.0742358078603, Blast_Score=81, Evalue=2e-15,
Organism=Drosophila melanogaster, GI24586597, Length=225, Percent_Identity=27.5555555555556, Blast_Score=80, Evalue=5e-15,
Organism=Drosophila melanogaster, GI24583745, Length=225, Percent_Identity=27.1111111111111, Blast_Score=79, Evalue=8e-15,
Organism=Drosophila melanogaster, GI24586593, Length=235, Percent_Identity=29.3617021276596, Blast_Score=79, Evalue=1e-14,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013780
- InterPro:   IPR006047
- InterPro:   IPR004185
- InterPro:   IPR006589
- InterPro:   IPR017853
- InterPro:   IPR013781
- InterPro:   IPR013783 [H]

Pfam domain/function: PF00128 Alpha-amylase; PF02903 Alpha-amylase_N [H]

EC number: 3.2.1.20 [C]

Molecular weight: Translated: 68465; Mature: 68465

Theoretical pI: Translated: 4.58; Mature: 4.58

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
2.4 %Met     (Translated Protein)
2.9 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
2.4 %Met     (Mature Protein)
2.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MEKAGIYHQPASSYAYSYDAKTLHIRIRTKRLDISEVTLIAADPYLWKDGKWQSESYTMR
CCCCCCCCCCCCCCEEEECCEEEEEEEEEEECCCCCEEEEEECCEEECCCCCCCCCHHHH
KIAETEEHDYWFFAITPEHRRLQYGFLLTDTEGETTFYGGRGFFEPTEANLATMDYYFKF
HHHCCCCCCEEEEEECCCCCEEEEEEEEECCCCCEEEECCCCCCCCCCCCEEEEEEEEEC
PFIHAVDTFEAPEWVGNTIWYQIFPERFANGNPAISPENALPWGSKDPNTTDFFGGDIEG
CEEEEECCCCCCHHHCCCEEEEECHHHHCCCCCCCCCCCCCCCCCCCCCCCCCCCCCHHH
IIEHLDYLAELGINGVYLTPVFEAPTNHKYDTVDYKKIDPHFGDKETFRKLVQEAHKRGI
HHHHHHHHHHHCCCCEEEEEEEECCCCCCCCCCCCEECCCCCCCHHHHHHHHHHHHHCCC
RIMLDAVFNHIGDTSAEWQDVVLNEEKSAYRDWFHIHSFPVRQNENGNIEGEPTLSYDTF
EEEHHHHHHHHCCCCCCHHHHEECCCHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCEE
AFTTHMPKLNTANPEVQKYLLDIATYWIREFDIDGWRLDVANEVDHAFWKEFKKAVQAEK
EEEECCCCCCCCCHHHHHHHHHHHHHHHHHCCCCCEEEECCHHHHHHHHHHHHHHHCCCC
EDIYILGEIWHDSWIWLLGDEFHAVMNYPFTQTIIENFIEEKITPEQMVSGINEQYMRYP
CCEEEEEEEECCCEEEEECCCCHHHHCCCHHHHHHHHHHHHCCCHHHHHHCCCHHHHHCC
NQVNEVMFNMLDSHDTARILTRANNDEDKVKQALAFMFAHTGSPCIYYGTEIGMDGGNDP
HHHHHHHHHHHCCCCCEEEEECCCCCHHHHHHHHHHHHHHCCCCEEEECCEECCCCCCCH
GCRKCMEWDETKQNQDMLAFTKQLIALRKDNQAIITSGELTWLGASSETGITAFSKELNG
HHHHHHCCHHCCCCCHHHHHHHHHHHHCCCCCEEEECCCEEEECCCCCCCCHHHHHHCCC
EKLHFLFNQAKEDKDFTISFENTATDIWNNRAVTENLVVPAKGFLVIKENS
CEEEEEHHHCCCCCCEEEEECCCHHHHHCCCCCCCCEEECCCCEEEEECCC
>Mature Secondary Structure
MEKAGIYHQPASSYAYSYDAKTLHIRIRTKRLDISEVTLIAADPYLWKDGKWQSESYTMR
CCCCCCCCCCCCCCEEEECCEEEEEEEEEEECCCCCEEEEEECCEEECCCCCCCCCHHHH
KIAETEEHDYWFFAITPEHRRLQYGFLLTDTEGETTFYGGRGFFEPTEANLATMDYYFKF
HHHCCCCCCEEEEEECCCCCEEEEEEEEECCCCCEEEECCCCCCCCCCCCEEEEEEEEEC
PFIHAVDTFEAPEWVGNTIWYQIFPERFANGNPAISPENALPWGSKDPNTTDFFGGDIEG
CEEEEECCCCCCHHHCCCEEEEECHHHHCCCCCCCCCCCCCCCCCCCCCCCCCCCCCHHH
IIEHLDYLAELGINGVYLTPVFEAPTNHKYDTVDYKKIDPHFGDKETFRKLVQEAHKRGI
HHHHHHHHHHHCCCCEEEEEEEECCCCCCCCCCCCEECCCCCCCHHHHHHHHHHHHHCCC
RIMLDAVFNHIGDTSAEWQDVVLNEEKSAYRDWFHIHSFPVRQNENGNIEGEPTLSYDTF
EEEHHHHHHHHCCCCCCHHHHEECCCHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCEE
AFTTHMPKLNTANPEVQKYLLDIATYWIREFDIDGWRLDVANEVDHAFWKEFKKAVQAEK
EEEECCCCCCCCCHHHHHHHHHHHHHHHHHCCCCCEEEECCHHHHHHHHHHHHHHHCCCC
EDIYILGEIWHDSWIWLLGDEFHAVMNYPFTQTIIENFIEEKITPEQMVSGINEQYMRYP
CCEEEEEEEECCCEEEEECCCCHHHHCCCHHHHHHHHHHHHCCCHHHHHHCCCHHHHHCC
NQVNEVMFNMLDSHDTARILTRANNDEDKVKQALAFMFAHTGSPCIYYGTEIGMDGGNDP
HHHHHHHHHHHCCCCCEEEEECCCCCHHHHHHHHHHHHHHCCCCEEEECCEECCCCCCCH
GCRKCMEWDETKQNQDMLAFTKQLIALRKDNQAIITSGELTWLGASSETGITAFSKELNG
HHHHHHCCHHCCCCCHHHHHHHHHHHHCCCCCEEEECCCEEEECCCCCCCCHHHHHHCCC
EKLHFLFNQAKEDKDFTISFENTATDIWNNRAVTENLVVPAKGFLVIKENS
CEEEEEHHHCCCCCCEEEEECCCHHHHHCCCCCCCCEEECCCCEEEEECCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): {} [C]

Substrates: Maltose; H2O; 1,4-alpha-D-glucan [C]

Specific reaction: Maltose + H2O = 2 alpha-D-glucose H2O + 1,4-alpha-D-glucan = beta-D-glucose + amylose(n-1) [C]

General reaction: O-Glycosyl bond hydrolysis; Exohydrolysis [C]

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 9384377 [H]