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The map label for this gene is mdxE [H]

Identifier: 226224732

GI number: 226224732

Start: 2208167

End: 2209426

Strand: Reverse

Name: mdxE [H]

Synonym: Lm4b_02149

Alternate gene names: 226224732

Gene position: 2209426-2208167 (Counterclockwise)

Preceding gene: 226224733

Following gene: 226224731

Centisome position: 75.86

GC content: 36.83

Gene sequence:

>1260_bases
ATGAAGCGTTTCAAAAAAGTGGGAATAGTTTCGGCGGTTTTAGTAATGGCTTTAAGCTTAGCAGCATGTGGTGGTGGAAA
AGACACTAGTAAATCTGGCTCATCTGATGAAAAAACATTAACGGTTTCCGTTGACGCAGGCTACAAAGACTACGTGAACA
AAATAAAAGGTGATTTTGAAAAAGATAATGACGTGAAAGTAAAAGTAGTCGAAAAAGACATGTTCGAAACATTAGAAGCA
TTGCCACTTGATGGCCCGGCTGGAACTGCTCCAGACGTAATGATGTCCGCATTTGATAGAATTGGAAGCTTAGGCCAACA
AGGACATTTAGCGGAAGTAAAACTTGGAAATAAAGACGATTACGATGAAAAAGACCAAAAACAAGTAACCATTGACGATA
AAATTTATGGCGCTCCAGCAATTATTGAAACTCTAGTACTTTACTATAATAAAGATTTACTCGACAAAGCTCCAGCAACT
TTCAAAGATTTAGAAACACTTTCTAAAGATTCTCGTTTTGCCTTCACTTCTGAAAAAGGAAAAAATACTGGTTTCTTAGC
AAAATGGACTGATTTCTACTTCTCTTACGGACTACTTGCAGGATACGGTGGTTATGTATTCGGCGATGAAGGTACGAATC
CAAAAGACATCGGTTTAAACAATAAAGGTTCGGTTGAAGGAATTACTTATGCAACAAAATGGTTCCAAGATGTATGGCCA
AAAGGTATGCAAGACAATAAGAGTGCAGATGACTTTATCCAAGATCAATTTGTTAAAGGTAAAGCAGCGGCAATTCTAGG
CGGTCCATGGTCAGCAGCAAACTACAAAGAAGCAAAAATTAATTACGGCGTAGCAAAAATCCCGACACTAAACAATGGCA
AAGAATACTCTCCATTTGCAGGCGGTAAAGGTTGGGTTGTAAGTAACTATTCTAAAAACAAAGATGTAGCTCAAAAATGG
TTGGATTATGTAACTAACCAAAAAAATCAAGAAACTTTATATGATATGACAAATGAAGTACCAGCTAACTTAAAAGCACG
CGATACAGCGAAATCAAAAAATGATGAATTAACTAATGCTGTTATCGAACAATACAAAAATGCGCAACCAATGCCAAATA
TTCCAGAAATGTCGGAAGTTTGGACAGGTGCTGAAAACTTAATGTTTGATGCAGCTTCTGGTTCGAAAACACCGCAACAA
TCAGCAGATGATGCAGTAAAAGTAATTGAAGATAACGTAACGCAAAAATATACTAAATAA

Upstream 100 bases:

>100_bases
GTTAACCTAATTCACAAACTTTAAAAAGAATTGAAAAAAGATGGGGACTTAGAAATCAGACAGTAAAGGGCGACTTTTTC
ATAAAGAGGAGGAAAAGTAA

Downstream 100 bases:

>100_bases
TAATGATTTGGCGGGGCAAAGATAGCGCGCTCCGCCATTTTTTTCAACTTAGCTAGTGAATCTATATCTAGAATTGAGGG
TAACAAAATGAAACTAGAAC

Product: maltose/maltodextrin ABC-transporter (binding protein)

Products: ADP; phosphate; maltose [Cytoplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 419; Mature: 419

Protein sequence:

>419_residues
MKRFKKVGIVSAVLVMALSLAACGGGKDTSKSGSSDEKTLTVSVDAGYKDYVNKIKGDFEKDNDVKVKVVEKDMFETLEA
LPLDGPAGTAPDVMMSAFDRIGSLGQQGHLAEVKLGNKDDYDEKDQKQVTIDDKIYGAPAIIETLVLYYNKDLLDKAPAT
FKDLETLSKDSRFAFTSEKGKNTGFLAKWTDFYFSYGLLAGYGGYVFGDEGTNPKDIGLNNKGSVEGITYATKWFQDVWP
KGMQDNKSADDFIQDQFVKGKAAAILGGPWSAANYKEAKINYGVAKIPTLNNGKEYSPFAGGKGWVVSNYSKNKDVAQKW
LDYVTNQKNQETLYDMTNEVPANLKARDTAKSKNDELTNAVIEQYKNAQPMPNIPEMSEVWTGAENLMFDAASGSKTPQQ
SADDAVKVIEDNVTQKYTK

Sequences:

>Translated_419_residues
MKRFKKVGIVSAVLVMALSLAACGGGKDTSKSGSSDEKTLTVSVDAGYKDYVNKIKGDFEKDNDVKVKVVEKDMFETLEA
LPLDGPAGTAPDVMMSAFDRIGSLGQQGHLAEVKLGNKDDYDEKDQKQVTIDDKIYGAPAIIETLVLYYNKDLLDKAPAT
FKDLETLSKDSRFAFTSEKGKNTGFLAKWTDFYFSYGLLAGYGGYVFGDEGTNPKDIGLNNKGSVEGITYATKWFQDVWP
KGMQDNKSADDFIQDQFVKGKAAAILGGPWSAANYKEAKINYGVAKIPTLNNGKEYSPFAGGKGWVVSNYSKNKDVAQKW
LDYVTNQKNQETLYDMTNEVPANLKARDTAKSKNDELTNAVIEQYKNAQPMPNIPEMSEVWTGAENLMFDAASGSKTPQQ
SADDAVKVIEDNVTQKYTK
>Mature_419_residues
MKRFKKVGIVSAVLVMALSLAACGGGKDTSKSGSSDEKTLTVSVDAGYKDYVNKIKGDFEKDNDVKVKVVEKDMFETLEA
LPLDGPAGTAPDVMMSAFDRIGSLGQQGHLAEVKLGNKDDYDEKDQKQVTIDDKIYGAPAIIETLVLYYNKDLLDKAPAT
FKDLETLSKDSRFAFTSEKGKNTGFLAKWTDFYFSYGLLAGYGGYVFGDEGTNPKDIGLNNKGSVEGITYATKWFQDVWP
KGMQDNKSADDFIQDQFVKGKAAAILGGPWSAANYKEAKINYGVAKIPTLNNGKEYSPFAGGKGWVVSNYSKNKDVAQKW
LDYVTNQKNQETLYDMTNEVPANLKARDTAKSKNDELTNAVIEQYKNAQPMPNIPEMSEVWTGAENLMFDAASGSKTPQQ
SADDAVKVIEDNVTQKYTK

Specific function: Part of the ABC transporter complex involved in maltodextrin import. Binds maltodextrin. Can also bind maltose with low affinity, but is not involved in its uptake [H]

COG id: COG2182

COG function: function code G; Maltose-binding periplasmic proteins/domains

Gene ontology:

Cell location: Cell membrane; Lipid-anchor (Potential) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the bacterial solute-binding protein 1 family [H]

Homologues:

Organism=Escherichia coli, GI1790466, Length=423, Percent_Identity=28.8416075650118, Blast_Score=144, Evalue=1e-35,

Paralogues:

None

Copy number: 660 Molecules/Cell In: Growth-Phase, Minimal-Media (Based on E. coli). 720 Molecules/Cell In: Stationary Phase, Rich Media (Based on E. coli). 240 Molecules/Cell In: Stationary Phase, Rich Media (Based on E. coli). 20 Molecules/Cell In: Stationary Phase,

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR006060
- InterPro:   IPR006059 [H]

Pfam domain/function: PF01547 SBP_bac_1 [H]

EC number: NA

Molecular weight: Translated: 46020; Mature: 46020

Theoretical pI: Translated: 4.94; Mature: 4.94

Prosite motif: PS00013 PROKAR_LIPOPROTEIN

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.2 %Cys     (Translated Protein)
2.4 %Met     (Translated Protein)
2.6 %Cys+Met (Translated Protein)
0.2 %Cys     (Mature Protein)
2.4 %Met     (Mature Protein)
2.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKRFKKVGIVSAVLVMALSLAACGGGKDTSKSGSSDEKTLTVSVDAGYKDYVNKIKGDFE
CCHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCEEEEEEECCCHHHHHHHHCCCCC
KDNDVKVKVVEKDMFETLEALPLDGPAGTAPDVMMSAFDRIGSLGQQGHLAEVKLGNKDD
CCCCCEEEEECHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHCCCCCCCEEEEEECCCCC
YDEKDQKQVTIDDKIYGAPAIIETLVLYYNKDLLDKAPATFKDLETLSKDSRFAFTSEKG
CCCCCCCEEEECCCCCCCHHHHHHHHHHHCHHHHHCCCCHHHHHHHHCCCCCEEEECCCC
KNTGFLAKWTDFYFSYGLLAGYGGYVFGDEGTNPKDIGLNNKGSVEGITYATKWFQDVWP
CCCCEEEHHHHHHHHHHHHHCCCCEEECCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHCC
KGMQDNKSADDFIQDQFVKGKAAAILGGPWSAANYKEAKINYGVAKIPTLNNGKEYSPFA
CCCCCCCCHHHHHHHHHHCCCEEEEECCCCCCCCCCCCEECCCEEECCCCCCCCCCCCCC
GGKGWVVSNYSKNKDVAQKWLDYVTNQKNQETLYDMTNEVPANLKARDTAKSKNDELTNA
CCCCCEEECCCCCHHHHHHHHHHHHCCCCHHHHHHHHHCCCCCCCCCCCCCCCCHHHHHH
VIEQYKNAQPMPNIPEMSEVWTGAENLMFDAASGSKTPQQSADDAVKVIEDNVTQKYTK
HHHHHHCCCCCCCCCCHHHHHCCHHHHEEECCCCCCCCCCCHHHHHHHHHHHHHHHCCC
>Mature Secondary Structure
MKRFKKVGIVSAVLVMALSLAACGGGKDTSKSGSSDEKTLTVSVDAGYKDYVNKIKGDFE
CCHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCEEEEEEECCCHHHHHHHHCCCCC
KDNDVKVKVVEKDMFETLEALPLDGPAGTAPDVMMSAFDRIGSLGQQGHLAEVKLGNKDD
CCCCCEEEEECHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHCCCCCCCEEEEEECCCCC
YDEKDQKQVTIDDKIYGAPAIIETLVLYYNKDLLDKAPATFKDLETLSKDSRFAFTSEKG
CCCCCCCEEEECCCCCCCHHHHHHHHHHHCHHHHHCCCCHHHHHHHHCCCCCEEEECCCC
KNTGFLAKWTDFYFSYGLLAGYGGYVFGDEGTNPKDIGLNNKGSVEGITYATKWFQDVWP
CCCCEEEHHHHHHHHHHHHHCCCCEEECCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHCC
KGMQDNKSADDFIQDQFVKGKAAAILGGPWSAANYKEAKINYGVAKIPTLNNGKEYSPFA
CCCCCCCCHHHHHHHHHHCCCEEEEECCCCCCCCCCCCEECCCEEECCCCCCCCCCCCCC
GGKGWVVSNYSKNKDVAQKWLDYVTNQKNQETLYDMTNEVPANLKARDTAKSKNDELTNA
CCCCCEEECCCCCHHHHHHHHHHHHCCCCHHHHHHHHHCCCCCCCCCCCCCCCCHHHHHH
VIEQYKNAQPMPNIPEMSEVWTGAENLMFDAASGSKTPQQSADDAVKVIEDNVTQKYTK
HHHHHHCCCCCCCCCCHHHHHCCHHHHEEECCCCCCCCCCCHHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: ATP; maltose [Periplasm]; H2O [C]

Specific reaction: ATP + maltose [Periplasm] + H2O = ADP + phosphate + maltose [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 9384377 [H]