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The map label for this gene is lacC [H]

Identifier: 226224700

GI number: 226224700

Start: 2174124

End: 2175056

Strand: Reverse

Name: lacC [H]

Synonym: Lm4b_02116

Alternate gene names: 226224700

Gene position: 2175056-2174124 (Counterclockwise)

Preceding gene: 226224701

Following gene: 226224699

Centisome position: 74.68

GC content: 39.44

Gene sequence:

>933_bases
ATGATTTACACGATAACATTAAATCCAGCCATTGACCGGTTGCTTTTCATTCAAGGAGAGTTAGAAAAAAGGAAGACTAA
TCGAGTCAAAAAAACGGAATTTGATTGCGGGGGAAAGGGGCTTCATGTTTCGGGAGTGTTATCCAAATTCGGTATTAAAA
ATGAAGCACTTGGAATTGCGGGATCAGACAATCTCGACAAACTATATGCCATTCTAAAAGAAAAACATATCAACCATGAT
TTCCTTGTAGAAGCTGGAACTTCGACAAGAGAATGCTTCGTCGTCTTAAGTGATGACACGAATGGCAGCACGATGATACC
TGAAGCTGGTTTTACCGTAAGTCAGACCAACAAAGACAATCTTTTAAAACAAATCGCTAAAAAAGTTAAAAAAGAGGATA
TGGTGGTGATTGCTGGATCTCCGCCTCCTCATTATACATTATCTGATTTTAAAGAACTACTAAGAACTGTCAAAGCAACA
GGAGCGTTTCTCGGATGTGATAATTCTGGTGAATATTTAAAATTAGCGGTCGAAATGGGCGTTGATTTCATTAAACCAAA
TGAAGATGAAGTCATTGCCATTTTGGATGAAACGACCAATTCACTAGAAGAAAATGTCCGCAAATTAGCCGGAAAAATTC
CCTATCTAGTCGTTTCGCTTGGAGCAAAAGGATCACTTTGCGCGCATAATGGCAAGTTGTATCAAGTTATTCCACCAAAA
GTGCAGGAACGCAATGATACTGGGGCTGGCGATGTGTTTGTTGGGGCATTTATTGCAGGACTTGCGATGAACATGCCAAT
TACTGAAACATTAAAAGTCGCGACAGGTTGTTCCGCTAGTAAAGTCATGCAACAAGACAGTTCGAGCTTTGATTTAGAAG
CTGCTGGAAAACTCAAGAATCAAGTAAGCATTATACAATTGGAGGAGAGATAA

Upstream 100 bases:

>100_bases
TCGCAATTTGGGTAGTATTACTAGTATTCTATGTGAAAATGATGAAAAAACGAACAATTGAACTAGAAAAAGCTAGCTCT
TAAGAAATAGGAGTGATGAG

Downstream 100 bases:

>100_bases
TATGTTATACCAAAAAGAACGTGAAGATTTAGCAAAAATAGTGAAGACGATGTTTGACCGCTTTGAAACGAATGCAGCTG
GAGGAAATGTAAGTGTGCGC

Product: 1-phosphofructokinase

Products: NA

Alternate protein names: Phosphotagatokinase [H]

Number of amino acids: Translated: 310; Mature: 310

Protein sequence:

>310_residues
MIYTITLNPAIDRLLFIQGELEKRKTNRVKKTEFDCGGKGLHVSGVLSKFGIKNEALGIAGSDNLDKLYAILKEKHINHD
FLVEAGTSTRECFVVLSDDTNGSTMIPEAGFTVSQTNKDNLLKQIAKKVKKEDMVVIAGSPPPHYTLSDFKELLRTVKAT
GAFLGCDNSGEYLKLAVEMGVDFIKPNEDEVIAILDETTNSLEENVRKLAGKIPYLVVSLGAKGSLCAHNGKLYQVIPPK
VQERNDTGAGDVFVGAFIAGLAMNMPITETLKVATGCSASKVMQQDSSSFDLEAAGKLKNQVSIIQLEER

Sequences:

>Translated_310_residues
MIYTITLNPAIDRLLFIQGELEKRKTNRVKKTEFDCGGKGLHVSGVLSKFGIKNEALGIAGSDNLDKLYAILKEKHINHD
FLVEAGTSTRECFVVLSDDTNGSTMIPEAGFTVSQTNKDNLLKQIAKKVKKEDMVVIAGSPPPHYTLSDFKELLRTVKAT
GAFLGCDNSGEYLKLAVEMGVDFIKPNEDEVIAILDETTNSLEENVRKLAGKIPYLVVSLGAKGSLCAHNGKLYQVIPPK
VQERNDTGAGDVFVGAFIAGLAMNMPITETLKVATGCSASKVMQQDSSSFDLEAAGKLKNQVSIIQLEER
>Mature_310_residues
MIYTITLNPAIDRLLFIQGELEKRKTNRVKKTEFDCGGKGLHVSGVLSKFGIKNEALGIAGSDNLDKLYAILKEKHINHD
FLVEAGTSTRECFVVLSDDTNGSTMIPEAGFTVSQTNKDNLLKQIAKKVKKEDMVVIAGSPPPHYTLSDFKELLRTVKAT
GAFLGCDNSGEYLKLAVEMGVDFIKPNEDEVIAILDETTNSLEENVRKLAGKIPYLVVSLGAKGSLCAHNGKLYQVIPPK
VQERNDTGAGDVFVGAFIAGLAMNMPITETLKVATGCSASKVMQQDSSSFDLEAAGKLKNQVSIIQLEER

Specific function: Key control step of glycolysis. [C]

COG id: COG1105

COG function: function code G; Fructose-1-phosphate kinase and related fructose-6-phosphate kinase (PfkB)

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the carbohydrate kinase pfkB family. LacC subfamily [H]

Homologues:

Organism=Escherichia coli, GI1788017, Length=293, Percent_Identity=28.6689419795222, Blast_Score=102, Evalue=2e-23,
Organism=Escherichia coli, GI1788493, Length=275, Percent_Identity=29.0909090909091, Blast_Score=100, Evalue=2e-22,

Paralogues:

None

Copy number: 496 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR011611
- InterPro:   IPR002173
- InterPro:   IPR005926
- InterPro:   IPR017583 [H]

Pfam domain/function: PF00294 PfkB [H]

EC number: =2.7.1.144 [H]

Molecular weight: Translated: 33669; Mature: 33669

Theoretical pI: Translated: 6.37; Mature: 6.37

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.6 %Cys     (Translated Protein)
2.3 %Met     (Translated Protein)
3.9 %Cys+Met (Translated Protein)
1.6 %Cys     (Mature Protein)
2.3 %Met     (Mature Protein)
3.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MIYTITLNPAIDRLLFIQGELEKRKTNRVKKTEFDCGGKGLHVSGVLSKFGIKNEALGIA
CEEEEEECHHHCEEEEEECCHHHHHHCCCCCCCCCCCCCCEEHHHHHHHCCCCCCCEEEC
GSDNLDKLYAILKEKHINHDFLVEAGTSTRECFVVLSDDTNGSTMIPEAGFTVSQTNKDN
CCCCHHHHHHHHHHHCCCCEEEEECCCCCCEEEEEEECCCCCCEECCCCCCEEECCCHHH
LLKQIAKKVKKEDMVVIAGSPPPHYTLSDFKELLRTVKATGAFLGCDNSGEYLKLAVEMG
HHHHHHHHHCCCCEEEEECCCCCCCCHHHHHHHHHHHHHCCCEEEECCCCCEEEEEEECC
VDFIKPNEDEVIAILDETTNSLEENVRKLAGKIPYLVVSLGAKGSLCAHNGKLYQVIPPK
CEEECCCCCCEEEEECCCHHHHHHHHHHHHCCCCEEEEEECCCCCEEECCCEEEEECCCC
VQERNDTGAGDVFVGAFIAGLAMNMPITETLKVATGCSASKVMQQDSSSFDLEAAGKLKN
CCCCCCCCCCHHHHHHHHHHHHCCCCHHHHHHHHCCCCHHHHHHCCCCCCCCHHHHCCCC
QVSIIQLEER
CEEEEEEECC
>Mature Secondary Structure
MIYTITLNPAIDRLLFIQGELEKRKTNRVKKTEFDCGGKGLHVSGVLSKFGIKNEALGIA
CEEEEEECHHHCEEEEEECCHHHHHHCCCCCCCCCCCCCCEEHHHHHHHCCCCCCCEEEC
GSDNLDKLYAILKEKHINHDFLVEAGTSTRECFVVLSDDTNGSTMIPEAGFTVSQTNKDN
CCCCHHHHHHHHHHHCCCCEEEEECCCCCCEEEEEEECCCCCCEECCCCCCEEECCCHHH
LLKQIAKKVKKEDMVVIAGSPPPHYTLSDFKELLRTVKATGAFLGCDNSGEYLKLAVEMG
HHHHHHHHHCCCCEEEEECCCCCCCCHHHHHHHHHHHHHCCCEEEECCCCCEEEEEEECC
VDFIKPNEDEVIAILDETTNSLEENVRKLAGKIPYLVVSLGAKGSLCAHNGKLYQVIPPK
CEEECCCCCCEEEEECCCHHHHHHHHHHHHCCCCEEEEEECCCCCEEECCCEEEEECCCC
VQERNDTGAGDVFVGAFIAGLAMNMPITETLKVATGCSASKVMQQDSSSFDLEAAGKLKN
CCCCCCCCCCHHHHHHHHHHHHCCCCHHHHHHHHCCCCHHHHHHCCCCCCCCHHHHCCCC
QVSIIQLEER
CEEEEEEECC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA