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The map label for this gene is alsS

Identifier: 226224604

GI number: 226224604

Start: 2066873

End: 2068570

Strand: Reverse

Name: alsS

Synonym: Lm4b_02018

Alternate gene names: 226224604

Gene position: 2068570-2066873 (Counterclockwise)

Preceding gene: 226224605

Following gene: 226224603

Centisome position: 71.02

GC content: 43.82

Gene sequence:

>1698_bases
ATGGCGAAACTAGAAAAAGACCAAGAAAAAGTAATAACACAAGGAAAATCAGGAGCGGATTTAGTTGTAGACAGCTTAAT
TAATCAAGGTGTTACGCATGTATTCGGGATTCCAGGAGCAAAAATTGATAAAGTTTTCGATGTGATGGAAGAACGTGGAC
CAGAATTAATTGTCAGTCGTCATGAGCAAAATGCGGCGTTTATGGCTGCCGCTATCGGTCGTCTAACCGGAAAACCTGGT
GTTGTACTTGTAACTAGTGGACCCGGCGCATCGAACCTTGCCACTGGACTTGTAACAGCAACTGCTGAAGGAGATCCAGT
CGTTGCGATAGCTGGAAATGTAACCAGGCAAGACCGCCTTAAAAAAACCCATCAATCAATGGATAATGCAGCACTTTTCC
GTCCAATTACAAAATATAGCGAAGAAGTAGTTCACGCTGAAAGTATCCCGGAAGCAATCACTAACGCTTTTCGCTCGGCG
GCAGAACCAAACCAAGGCGCTGCTTTTGTCAGTTTGCCACAAGATATCGTGAATGAACCAAACGTACCAGTAAAAGCGAT
TCGCCCACTTGCTAAACCAGAAAATGGTCCAGCTTCCAAAGAACAAGTTGCGAAACTCGTTACTCGTTTGAAAAAAGCGA
AATTACCAGTATTGCTATTAGGTATGCGAGCATCTAGCCCGGAAGTAACTGGTGCGATTCGTCGGCTACTCCAAAAAACA
AGCATCCCAGTTGTAGAAACCTTCCAAGCAGCTGGTGTCATCTCACGTGATTTAGAAGATAATTTCTTTGGGCGTGTAGG
TCTCTTCCGCAACCAACCAGGGGATATTTTGCTAAATAAAGCTGATTTAGTTATTACAGTAGGTTACGATCCAATTGAAT
ATGATCCAAAAGCTTGGAATGCTTCGGGTGATAGAACGATTGTACATTTAGATGACATCCGAGCAGATATTGACCACTAC
TATCAACCTGTGACAGAATTAGTCGGAAACATCGCGCTTACTTTAGACCGAGTGAATGCGAAATTCAGCGGTTTAGAATT
AGCGGAAAAAGAACTTGAAACCTTAAAAGAACTTCATGCTCAACTAGAAGAGCGAGATGTTCCACCAGAAAGTGATGAAA
CTAACCGAGTACATCCGTTGTCGGTCATTCAAACGCTGCGTTCGGCAATTGATGACAACGTAACTGTGACAGTCGACGTT
GGTTCACATTATATTTGGATGGCACGTCATTTCCGGTCCTATGAACCACGCCGTCTGCTTTTCAGTAACGGTATGCAAAC
GCTTGGTGTCGCGCTTCCTTGGGGGATTGCTGCAACACTCGTACATCCGGGTGAAAAAGTGGTTTCGATTTCTGGTGACG
GTGGCTTCCTATTTTCTGCCATGGAATTAGAAACAGCTGTCCGCTTGCGTGCGCCACTTGTGCACCTTGTATGGAATGAC
GGTAGCTATGACATGGTCGCCTTCCAACAAAAAATGAAATACGGCAAAGAAGCAGCAGTTCGTTTTGGTGATGTCGATAT
TGTTAAATTCGCCGAAAGCTTCGGTGCAAAAGGACTTCGCGTAACTAAACCGTCAGAACTTAGTGATGTGTTAAAAGAAG
CCCTTGAAACAGAAGGTCCCGTCGTTGTAGATATTCCAATTGATTACCGTGATAACATCAAACTCGGCGAAACTTTACTA
CCTGACCAATTTTATTAA

Upstream 100 bases:

>100_bases
AAAATTCTTTTGAGTAGTATAATTAGTCTTGTGAATGAGGTTTTTATAAAATCTGTACTATTAAAATTTAAATAATTTAT
ATAACAAAAGGATGATAACG

Downstream 100 bases:

>100_bases
AACGTTAAAAATCTCTCGGATTTCCCGAGAGATTTTTTTATAGTTAAAGGTTGACTTGGAGTGGACTTCAAGGTTTATGC
TGTAATAGTACTAGAAATTA

Product: acetolactate synthase

Products: NA

Alternate protein names: ALS; Acetohydroxy-acid synthase [H]

Number of amino acids: Translated: 565; Mature: 564

Protein sequence:

>565_residues
MAKLEKDQEKVITQGKSGADLVVDSLINQGVTHVFGIPGAKIDKVFDVMEERGPELIVSRHEQNAAFMAAAIGRLTGKPG
VVLVTSGPGASNLATGLVTATAEGDPVVAIAGNVTRQDRLKKTHQSMDNAALFRPITKYSEEVVHAESIPEAITNAFRSA
AEPNQGAAFVSLPQDIVNEPNVPVKAIRPLAKPENGPASKEQVAKLVTRLKKAKLPVLLLGMRASSPEVTGAIRRLLQKT
SIPVVETFQAAGVISRDLEDNFFGRVGLFRNQPGDILLNKADLVITVGYDPIEYDPKAWNASGDRTIVHLDDIRADIDHY
YQPVTELVGNIALTLDRVNAKFSGLELAEKELETLKELHAQLEERDVPPESDETNRVHPLSVIQTLRSAIDDNVTVTVDV
GSHYIWMARHFRSYEPRRLLFSNGMQTLGVALPWGIAATLVHPGEKVVSISGDGGFLFSAMELETAVRLRAPLVHLVWND
GSYDMVAFQQKMKYGKEAAVRFGDVDIVKFAESFGAKGLRVTKPSELSDVLKEALETEGPVVVDIPIDYRDNIKLGETLL
PDQFY

Sequences:

>Translated_565_residues
MAKLEKDQEKVITQGKSGADLVVDSLINQGVTHVFGIPGAKIDKVFDVMEERGPELIVSRHEQNAAFMAAAIGRLTGKPG
VVLVTSGPGASNLATGLVTATAEGDPVVAIAGNVTRQDRLKKTHQSMDNAALFRPITKYSEEVVHAESIPEAITNAFRSA
AEPNQGAAFVSLPQDIVNEPNVPVKAIRPLAKPENGPASKEQVAKLVTRLKKAKLPVLLLGMRASSPEVTGAIRRLLQKT
SIPVVETFQAAGVISRDLEDNFFGRVGLFRNQPGDILLNKADLVITVGYDPIEYDPKAWNASGDRTIVHLDDIRADIDHY
YQPVTELVGNIALTLDRVNAKFSGLELAEKELETLKELHAQLEERDVPPESDETNRVHPLSVIQTLRSAIDDNVTVTVDV
GSHYIWMARHFRSYEPRRLLFSNGMQTLGVALPWGIAATLVHPGEKVVSISGDGGFLFSAMELETAVRLRAPLVHLVWND
GSYDMVAFQQKMKYGKEAAVRFGDVDIVKFAESFGAKGLRVTKPSELSDVLKEALETEGPVVVDIPIDYRDNIKLGETLL
PDQFY
>Mature_564_residues
AKLEKDQEKVITQGKSGADLVVDSLINQGVTHVFGIPGAKIDKVFDVMEERGPELIVSRHEQNAAFMAAAIGRLTGKPGV
VLVTSGPGASNLATGLVTATAEGDPVVAIAGNVTRQDRLKKTHQSMDNAALFRPITKYSEEVVHAESIPEAITNAFRSAA
EPNQGAAFVSLPQDIVNEPNVPVKAIRPLAKPENGPASKEQVAKLVTRLKKAKLPVLLLGMRASSPEVTGAIRRLLQKTS
IPVVETFQAAGVISRDLEDNFFGRVGLFRNQPGDILLNKADLVITVGYDPIEYDPKAWNASGDRTIVHLDDIRADIDHYY
QPVTELVGNIALTLDRVNAKFSGLELAEKELETLKELHAQLEERDVPPESDETNRVHPLSVIQTLRSAIDDNVTVTVDVG
SHYIWMARHFRSYEPRRLLFSNGMQTLGVALPWGIAATLVHPGEKVVSISGDGGFLFSAMELETAVRLRAPLVHLVWNDG
SYDMVAFQQKMKYGKEAAVRFGDVDIVKFAESFGAKGLRVTKPSELSDVLKEALETEGPVVVDIPIDYRDNIKLGETLLP
DQFY

Specific function: Valine and isoleucine biosynthesis; first step. [C]

COG id: COG0028

COG function: function code EH; Thiamine pyrophosphate-requiring enzymes [acetolactate synthase, pyruvate dehydrogenase (cytochrome), glyoxylate carboligase, phosphonopyruvate decarboxylase]

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the TPP enzyme family [H]

Homologues:

Organism=Homo sapiens, GI93004078, Length=578, Percent_Identity=27.1626297577855, Blast_Score=181, Evalue=1e-45,
Organism=Homo sapiens, GI21361361, Length=592, Percent_Identity=26.5202702702703, Blast_Score=166, Evalue=4e-41,
Organism=Escherichia coli, GI87081685, Length=554, Percent_Identity=29.7833935018051, Blast_Score=234, Evalue=1e-62,
Organism=Escherichia coli, GI1790104, Length=546, Percent_Identity=28.021978021978, Blast_Score=214, Evalue=1e-56,
Organism=Escherichia coli, GI1786717, Length=564, Percent_Identity=27.1276595744681, Blast_Score=167, Evalue=2e-42,
Organism=Escherichia coli, GI1787096, Length=546, Percent_Identity=26.9230769230769, Blast_Score=163, Evalue=3e-41,
Organism=Escherichia coli, GI1788716, Length=554, Percent_Identity=25.812274368231, Blast_Score=138, Evalue=1e-33,
Organism=Caenorhabditis elegans, GI17531299, Length=550, Percent_Identity=27.8181818181818, Blast_Score=146, Evalue=2e-35,
Organism=Caenorhabditis elegans, GI17531301, Length=550, Percent_Identity=27.8181818181818, Blast_Score=146, Evalue=3e-35,
Organism=Caenorhabditis elegans, GI17542570, Length=571, Percent_Identity=25.9194395796848, Blast_Score=137, Evalue=1e-32,
Organism=Saccharomyces cerevisiae, GI6323755, Length=573, Percent_Identity=28.9703315881326, Blast_Score=207, Evalue=4e-54,
Organism=Saccharomyces cerevisiae, GI6320816, Length=542, Percent_Identity=27.1217712177122, Blast_Score=134, Evalue=5e-32,
Organism=Saccharomyces cerevisiae, GI6323163, Length=591, Percent_Identity=22.5042301184433, Blast_Score=67, Evalue=1e-11,
Organism=Saccharomyces cerevisiae, GI6323073, Length=540, Percent_Identity=22.2222222222222, Blast_Score=64, Evalue=5e-11,
Organism=Drosophila melanogaster, GI19922626, Length=565, Percent_Identity=26.1946902654867, Blast_Score=154, Evalue=1e-37,

Paralogues:

None

Copy number: 340 Molecules/Cell In: Growth-Phase, Minimal-Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR012782
- InterPro:   IPR012000
- InterPro:   IPR012001
- InterPro:   IPR000399
- InterPro:   IPR011766 [H]

Pfam domain/function: PF02775 TPP_enzyme_C; PF00205 TPP_enzyme_M; PF02776 TPP_enzyme_N [H]

EC number: =2.2.1.6 [H]

Molecular weight: Translated: 61684; Mature: 61553

Theoretical pI: Translated: 5.42; Mature: 5.42

Prosite motif: PS00187 TPP_ENZYMES

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
1.8 %Met     (Translated Protein)
1.8 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
1.6 %Met     (Mature Protein)
1.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAKLEKDQEKVITQGKSGADLVVDSLINQGVTHVFGIPGAKIDKVFDVMEERGPELIVSR
CCCCCCHHHHHHHCCCCCHHHHHHHHHHCCCCEEECCCCCHHHHHHHHHHHCCCEEEEEC
HEQNAAFMAAAIGRLTGKPGVVLVTSGPGASNLATGLVTATAEGDPVVAIAGNVTRQDRL
CCCCHHHHHHHHHHHCCCCCEEEEECCCCCCHHHHHEEEEECCCCEEEEEECCCHHHHHH
KKTHQSMDNAALFRPITKYSEEVVHAESIPEAITNAFRSAAEPNQGAAFVSLPQDIVNEP
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCEEEECCHHHCCCC
NVPVKAIRPLAKPENGPASKEQVAKLVTRLKKAKLPVLLLGMRASSPEVTGAIRRLLQKT
CCCHHHHHHCCCCCCCCCCHHHHHHHHHHHHHCCCCEEEEECCCCCCHHHHHHHHHHHHC
SIPVVETFQAAGVISRDLEDNFFGRVGLFRNQPGDILLNKADLVITVGYDPIEYDPKAWN
CCCHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCEEEECCCEEEEECCCCCCCCCCCCC
ASGDRTIVHLDDIRADIDHYYQPVTELVGNIALTLDRVNAKFSGLELAEKELETLKELHA
CCCCEEEEEHHHHHHHHHHHHHHHHHHHHHHEEEEECCCCCCCCHHHHHHHHHHHHHHHH
QLEERDVPPESDETNRVHPLSVIQTLRSAIDDNVTVTVDVGSHYIWMARHFRSYEPRRLL
HHHHCCCCCCCCCCCCCCHHHHHHHHHHHHCCCCEEEEECCCCEEHHHHHHCCCCCCHHH
FSNGMQTLGVALPWGIAATLVHPGEKVVSISGDGGFLFSAMELETAVRLRAPLVHLVWND
HHCCHHHHHHHCCCHHHHHEECCCCEEEEEECCCCEEEEHHHHHHHHHHHCCEEEEEECC
GSYDMVAFQQKMKYGKEAAVRFGDVDIVKFAESFGAKGLRVTKPSELSDVLKEALETEGP
CCCHHHHHHHHHHCCHHHHEEECCHHHHHHHHHHCCCCEEECCCHHHHHHHHHHHHCCCC
VVVDIPIDYRDNIKLGETLLPDQFY
EEEEEECCCCCCCCCCCCCCCCCCC
>Mature Secondary Structure 
AKLEKDQEKVITQGKSGADLVVDSLINQGVTHVFGIPGAKIDKVFDVMEERGPELIVSR
CCCCCHHHHHHHCCCCCHHHHHHHHHHCCCCEEECCCCCHHHHHHHHHHHCCCEEEEEC
HEQNAAFMAAAIGRLTGKPGVVLVTSGPGASNLATGLVTATAEGDPVVAIAGNVTRQDRL
CCCCHHHHHHHHHHHCCCCCEEEEECCCCCCHHHHHEEEEECCCCEEEEEECCCHHHHHH
KKTHQSMDNAALFRPITKYSEEVVHAESIPEAITNAFRSAAEPNQGAAFVSLPQDIVNEP
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCEEEECCHHHCCCC
NVPVKAIRPLAKPENGPASKEQVAKLVTRLKKAKLPVLLLGMRASSPEVTGAIRRLLQKT
CCCHHHHHHCCCCCCCCCCHHHHHHHHHHHHHCCCCEEEEECCCCCCHHHHHHHHHHHHC
SIPVVETFQAAGVISRDLEDNFFGRVGLFRNQPGDILLNKADLVITVGYDPIEYDPKAWN
CCCHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCEEEECCCEEEEECCCCCCCCCCCCC
ASGDRTIVHLDDIRADIDHYYQPVTELVGNIALTLDRVNAKFSGLELAEKELETLKELHA
CCCCEEEEEHHHHHHHHHHHHHHHHHHHHHHEEEEECCCCCCCCHHHHHHHHHHHHHHHH
QLEERDVPPESDETNRVHPLSVIQTLRSAIDDNVTVTVDVGSHYIWMARHFRSYEPRRLL
HHHHCCCCCCCCCCCCCCHHHHHHHHHHHHCCCCEEEEECCCCEEHHHHHHCCCCCCHHH
FSNGMQTLGVALPWGIAATLVHPGEKVVSISGDGGFLFSAMELETAVRLRAPLVHLVWND
HHCCHHHHHHHCCCHHHHHEECCCCEEEEEECCCCEEEEHHHHHHHHHHHCCEEEEEECC
GSYDMVAFQQKMKYGKEAAVRFGDVDIVKFAESFGAKGLRVTKPSELSDVLKEALETEGP
CCCHHHHHHHHHHCCHHHHEEECCHHHHHHHHHHCCCCEEECCCHHHHHHHHHHHHCCCC
VVVDIPIDYRDNIKLGETLLPDQFY
EEEEEECCCCCCCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 7685336; 9353933; 9384377; 10809684 [H]