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The map label for this gene is pnp

Identifier: 226224558

GI number: 226224558

Start: 2021811

End: 2022629

Strand: Reverse

Name: pnp

Synonym: Lm4b_01972

Alternate gene names: 226224558

Gene position: 2022629-2021811 (Counterclockwise)

Preceding gene: 226224559

Following gene: 226224557

Centisome position: 69.44

GC content: 39.68

Gene sequence:

>819_bases
ATGAGTTTAGAAAAAGTAAATGAAGCAGTTGCGAAAATTAGAGAAAGTTACAATGGTACACCAAAAATTGGTTTGATTCT
TGGTTCAGGTTTAGGTGTGCTTGCGGATGAAGTAAACAATCCGACAAAACTTTCTTATAGCGAAATCCCACATTTTCCTG
TTTCGACAGTAGAAGGACATGCTGGCCAATTTGTTTTTGGTGAGTTGGAAAATAAAGAAGTTGTTGCAATGCAAGGACGT
TTCCACTTTTATGAAGGCTATTCAATGCAAGATGTTACTTTCCCAGTTCGTGTGATGAAAGAGCTTGGTGTAGAAGTGCT
TGTTGTGACGAATGCGGCAGGTGGCGTAAATGAATTATATTCTGCTGGAGACTTAATGTTGATTTCTGATCACATTAACT
TTACTGGAACGAACCCGCTTATTGGACCAAATGATGAGCATTTTGGACCGCGTTTCCCAGATATGTCCGAGGCGTATAAT
TTAGCTCTACGTGTGGATGCTAGACTTATTGCTCAAGAACTTAATTTGACTATCCGTGAAGGTGTTTATGCTGGATTTAG
CGGTCCAACTTACGAAACACCTGCTGAAATTCAAATGATGCGCACACTTGGTGCAGATGCAGTTGGGATGTCCACTGTAC
CGGAAGTAATCATTGCGAACCATGCTGGATTACGAGTGCTAGGTATTTCTTGTATTACCAATATGGCAGCCGGAATTCTG
GATCAACCACTTTCTCATACGGAAGTTATTGAAACAACAGATCAAGTTCGTAGCACGTTTTTACAATATGTAAAAGCTAT
TGTTGCTAAAATTTCTTAA

Upstream 100 bases:

>100_bases
CGATCTCGGCGCAACAGTTGCGGATAATTTTGAAGTGAAAATGCCTGAATATGGAACAAGTTTCTTAAAAGACTTGAAAT
AGGAAAAGGGAGGAACAAAA

Downstream 100 bases:

>100_bases
AATAATTGGTAGCTAATCTCAGTCCAAATTTGTTATTTTCTGAAGCAAGGAGATAATAGATTTGGGCTGAGTTTTTTACG
TATTTGATAATATTTTCAGA

Product: purine nucleoside phosphorylase

Products: NA

Alternate protein names: Inosine phosphorylase; Purine nucleoside phosphorylase I; PNP I; PU-NPase I [H]

Number of amino acids: Translated: 272; Mature: 271

Protein sequence:

>272_residues
MSLEKVNEAVAKIRESYNGTPKIGLILGSGLGVLADEVNNPTKLSYSEIPHFPVSTVEGHAGQFVFGELENKEVVAMQGR
FHFYEGYSMQDVTFPVRVMKELGVEVLVVTNAAGGVNELYSAGDLMLISDHINFTGTNPLIGPNDEHFGPRFPDMSEAYN
LALRVDARLIAQELNLTIREGVYAGFSGPTYETPAEIQMMRTLGADAVGMSTVPEVIIANHAGLRVLGISCITNMAAGIL
DQPLSHTEVIETTDQVRSTFLQYVKAIVAKIS

Sequences:

>Translated_272_residues
MSLEKVNEAVAKIRESYNGTPKIGLILGSGLGVLADEVNNPTKLSYSEIPHFPVSTVEGHAGQFVFGELENKEVVAMQGR
FHFYEGYSMQDVTFPVRVMKELGVEVLVVTNAAGGVNELYSAGDLMLISDHINFTGTNPLIGPNDEHFGPRFPDMSEAYN
LALRVDARLIAQELNLTIREGVYAGFSGPTYETPAEIQMMRTLGADAVGMSTVPEVIIANHAGLRVLGISCITNMAAGIL
DQPLSHTEVIETTDQVRSTFLQYVKAIVAKIS
>Mature_271_residues
SLEKVNEAVAKIRESYNGTPKIGLILGSGLGVLADEVNNPTKLSYSEIPHFPVSTVEGHAGQFVFGELENKEVVAMQGRF
HFYEGYSMQDVTFPVRVMKELGVEVLVVTNAAGGVNELYSAGDLMLISDHINFTGTNPLIGPNDEHFGPRFPDMSEAYNL
ALRVDARLIAQELNLTIREGVYAGFSGPTYETPAEIQMMRTLGADAVGMSTVPEVIIANHAGLRVLGISCITNMAAGILD
QPLSHTEVIETTDQVRSTFLQYVKAIVAKIS

Specific function: Cleavage of guanosine or inosine to respective bases and sugar-1-phosphate molecules [H]

COG id: COG0005

COG function: function code F; Purine nucleoside phosphorylase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the PNP/MTAP phosphorylase family [H]

Homologues:

Organism=Homo sapiens, GI157168362, Length=260, Percent_Identity=50.7692307692308, Blast_Score=255, Evalue=3e-68,
Organism=Homo sapiens, GI47132622, Length=220, Percent_Identity=29.0909090909091, Blast_Score=69, Evalue=5e-12,
Organism=Escherichia coli, GI1788746, Length=253, Percent_Identity=44.6640316205534, Blast_Score=233, Evalue=1e-62,
Organism=Caenorhabditis elegans, GI17541190, Length=275, Percent_Identity=43.6363636363636, Blast_Score=223, Evalue=1e-58,
Organism=Saccharomyces cerevisiae, GI6323238, Length=235, Percent_Identity=47.6595744680851, Blast_Score=204, Evalue=1e-53,
Organism=Drosophila melanogaster, GI45552885, Length=260, Percent_Identity=48.8461538461538, Blast_Score=246, Evalue=1e-65,
Organism=Drosophila melanogaster, GI24656090, Length=260, Percent_Identity=48.8461538461538, Blast_Score=246, Evalue=1e-65,
Organism=Drosophila melanogaster, GI45552887, Length=260, Percent_Identity=48.8461538461538, Blast_Score=246, Evalue=2e-65,
Organism=Drosophila melanogaster, GI24656093, Length=260, Percent_Identity=48.8461538461538, Blast_Score=246, Evalue=2e-65,
Organism=Drosophila melanogaster, GI24762376, Length=236, Percent_Identity=38.5593220338983, Blast_Score=145, Evalue=2e-35,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR011268
- InterPro:   IPR000845
- InterPro:   IPR011270
- InterPro:   IPR001369
- InterPro:   IPR018099 [H]

Pfam domain/function: PF01048 PNP_UDP_1 [H]

EC number: =2.4.2.1 [H]

Molecular weight: Translated: 29476; Mature: 29345

Theoretical pI: Translated: 4.60; Mature: 4.60

Prosite motif: PS01240 PNP_MTAP_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
3.7 %Met     (Translated Protein)
4.0 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
3.3 %Met     (Mature Protein)
3.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSLEKVNEAVAKIRESYNGTPKIGLILGSGLGVLADEVNNPTKLSYSEIPHFPVSTVEGH
CCHHHHHHHHHHHHHHCCCCCEEEEEEECCCHHHHHHCCCCCEECHHHCCCCCCCCCCCC
AGQFVFGELENKEVVAMQGRFHFYEGYSMQDVTFPVRVMKELGVEVLVVTNAAGGVNELY
CCCEEEEECCCCEEEEEECCEEEECCCCCCCCHHHHHHHHHCCCEEEEEECCCCCHHHHH
SAGDLMLISDHINFTGTNPLIGPNDEHFGPRFPDMSEAYNLALRVDARLIAQELNLTIRE
CCCCEEEEECCCCCCCCCCCCCCCCCCCCCCCCCHHHHHEEEEEHHHHHHHHHHCEEHEC
GVYAGFSGPTYETPAEIQMMRTLGADAVGMSTVPEVIIANHAGLRVLGISCITNMAAGIL
CEEECCCCCCCCCCHHHHHHHHHCCCCCCCHHHCHHEEECCCCEEEEHHHHHHHHHHHHH
DQPLSHTEVIETTDQVRSTFLQYVKAIVAKIS
CCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCC
>Mature Secondary Structure 
SLEKVNEAVAKIRESYNGTPKIGLILGSGLGVLADEVNNPTKLSYSEIPHFPVSTVEGH
CHHHHHHHHHHHHHHCCCCCEEEEEEECCCHHHHHHCCCCCEECHHHCCCCCCCCCCCC
AGQFVFGELENKEVVAMQGRFHFYEGYSMQDVTFPVRVMKELGVEVLVVTNAAGGVNELY
CCCEEEEECCCCEEEEEECCEEEECCCCCCCCHHHHHHHHHCCCEEEEEECCCCCHHHHH
SAGDLMLISDHINFTGTNPLIGPNDEHFGPRFPDMSEAYNLALRVDARLIAQELNLTIRE
CCCCEEEEECCCCCCCCCCCCCCCCCCCCCCCCCHHHHHEEEEEHHHHHHHHHHCEEHEC
GVYAGFSGPTYETPAEIQMMRTLGADAVGMSTVPEVIIANHAGLRVLGISCITNMAAGIL
CEEECCCCCCCCCCHHHHHHHHHCCCCCCCHHHCHHEEECCCCEEEEHHHHHHHHHHHHH
DQPLSHTEVIETTDQVRSTFLQYVKAIVAKIS
CCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 10537218; 8969508; 9384377; 1629150 [H]