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The map label for this gene is folE

Identifier: 226224537

GI number: 226224537

Start: 1995378

End: 1995947

Strand: Reverse

Name: folE

Synonym: Lm4b_01950

Alternate gene names: 226224537

Gene position: 1995947-1995378 (Counterclockwise)

Preceding gene: 226224538

Following gene: 226224536

Centisome position: 68.53

GC content: 38.42

Gene sequence:

>570_bases
ATGGAGCAAATAGACAAGCAAAAGATTGCTGACGCGGTAAAAGTTATTTTAGAAGCAGTAGGAGAAAATCCAGAACGTGA
AGGACTGATTGATACACCAATGCGAGTAGCTCGTATGTATGAAGAAGTTTTCGCTGGGCTGAAAAAAGATCCTTCTGTTC
ATTTCGATACTATTTTTGAAGAACAACATGAAGAATTAGTACTTGTGAAAGACATTCGCTTTTCGTCTATGTGTGAACAT
CACCTTGTCCCTTTTTTTGGAGTAGCTCATGTAGCATACTTACCACAAAATGGAAGAGTTGCTGGTCTTAGTAAACTTGC
TCGCGTAGTGGATGATGTAAGTCGCCGCCCGCAGTTACAAGAACGGATTACAACGACTGTTGCAGAAATTATGATGGAAA
AACTAAAACCTTTGGGTGTAATGGTCATCATGGAAGCAGAGCATATGTGCATGACTATCCGTGGTGTAAATAAACCTGGA
ACAAAAACAATTACAAGTGCTGTTCGTGGTGCTTTTAAAAATGACGATAAGCTTAGAAGTGAAGTTTTAGCTTTAATTAA
GCATAATTAA

Upstream 100 bases:

>100_bases
CTATTATTATCACTTATTCAAGCAAATTAAATTTACCTTGAAGTTTGGCTGTGTTAAGATATACAAGGTATATAATGCTA
AAAAGATAGGGGAATTAAGG

Downstream 100 bases:

>100_bases
ATATCCTTTATGGTATAATTTTTTACATAATAAGGAGATTCAAAGGGGATTGTTAAATGACTTATCAGGCAAGAGAGGCT
GGGCTAAAGGAAGTGGAGCA

Product: GTP cyclohydrolase I

Products: NA

Alternate protein names: GTP cyclohydrolase I; GTP-CH-I [H]

Number of amino acids: Translated: 189; Mature: 189

Protein sequence:

>189_residues
MEQIDKQKIADAVKVILEAVGENPEREGLIDTPMRVARMYEEVFAGLKKDPSVHFDTIFEEQHEELVLVKDIRFSSMCEH
HLVPFFGVAHVAYLPQNGRVAGLSKLARVVDDVSRRPQLQERITTTVAEIMMEKLKPLGVMVIMEAEHMCMTIRGVNKPG
TKTITSAVRGAFKNDDKLRSEVLALIKHN

Sequences:

>Translated_189_residues
MEQIDKQKIADAVKVILEAVGENPEREGLIDTPMRVARMYEEVFAGLKKDPSVHFDTIFEEQHEELVLVKDIRFSSMCEH
HLVPFFGVAHVAYLPQNGRVAGLSKLARVVDDVSRRPQLQERITTTVAEIMMEKLKPLGVMVIMEAEHMCMTIRGVNKPG
TKTITSAVRGAFKNDDKLRSEVLALIKHN
>Mature_189_residues
MEQIDKQKIADAVKVILEAVGENPEREGLIDTPMRVARMYEEVFAGLKKDPSVHFDTIFEEQHEELVLVKDIRFSSMCEH
HLVPFFGVAHVAYLPQNGRVAGLSKLARVVDDVSRRPQLQERITTTVAEIMMEKLKPLGVMVIMEAEHMCMTIRGVNKPG
TKTITSAVRGAFKNDDKLRSEVLALIKHN

Specific function: Tetrahydrofolate biosynthesis; first step. [C]

COG id: COG0302

COG function: function code H; GTP cyclohydrolase I

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the GTP cyclohydrolase I family [H]

Homologues:

Organism=Homo sapiens, GI66932968, Length=186, Percent_Identity=49.4623655913978, Blast_Score=178, Evalue=2e-45,
Organism=Homo sapiens, GI4503949, Length=186, Percent_Identity=49.4623655913978, Blast_Score=178, Evalue=2e-45,
Organism=Homo sapiens, GI66932970, Length=156, Percent_Identity=46.1538461538462, Blast_Score=135, Evalue=2e-32,
Organism=Homo sapiens, GI66932972, Length=145, Percent_Identity=48.9655172413793, Blast_Score=134, Evalue=4e-32,
Organism=Escherichia coli, GI1788476, Length=189, Percent_Identity=34.9206349206349, Blast_Score=103, Evalue=6e-24,
Organism=Caenorhabditis elegans, GI17560486, Length=181, Percent_Identity=44.7513812154696, Blast_Score=162, Evalue=7e-41,
Organism=Saccharomyces cerevisiae, GI6321706, Length=182, Percent_Identity=47.8021978021978, Blast_Score=172, Evalue=3e-44,
Organism=Drosophila melanogaster, GI24656782, Length=180, Percent_Identity=45.5555555555556, Blast_Score=155, Evalue=1e-38,
Organism=Drosophila melanogaster, GI24656796, Length=180, Percent_Identity=45.5555555555556, Blast_Score=154, Evalue=2e-38,
Organism=Drosophila melanogaster, GI24656791, Length=180, Percent_Identity=45.5555555555556, Blast_Score=154, Evalue=4e-38,

Paralogues:

None

Copy number: 420 Molecules/Cell In: Growth-Phase, Minimal-Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001474
- InterPro:   IPR020602
- InterPro:   IPR018234 [H]

Pfam domain/function: PF01227 GTP_cyclohydroI [H]

EC number: =3.5.4.16 [H]

Molecular weight: Translated: 21335; Mature: 21335

Theoretical pI: Translated: 6.89; Mature: 6.89

Prosite motif: PS00859 GTP_CYCLOHYDROL_1_1 ; PS00860 GTP_CYCLOHYDROL_1_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
5.3 %Met     (Translated Protein)
6.3 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
5.3 %Met     (Mature Protein)
6.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MEQIDKQKIADAVKVILEAVGENPEREGLIDTPMRVARMYEEVFAGLKKDPSVHFDTIFE
CCHHHHHHHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHHHCCCCCCCCHHHHHHH
EQHEELVLVKDIRFSSMCEHHLVPFFGVAHVAYLPQNGRVAGLSKLARVVDDVSRRPQLQ
HHHHCEEHHHHCCHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHCCHHHH
ERITTTVAEIMMEKLKPLGVMVIMEAEHMCMTIRGVNKPGTKTITSAVRGAFKNDDKLRS
HHHHHHHHHHHHHHCCCCCEEEEEECCHHHHHEECCCCCCHHHHHHHHHHHCCCHHHHHH
EVLALIKHN
HHHHHHHCC
>Mature Secondary Structure
MEQIDKQKIADAVKVILEAVGENPEREGLIDTPMRVARMYEEVFAGLKKDPSVHFDTIFE
CCHHHHHHHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHHHCCCCCCCCHHHHHHH
EQHEELVLVKDIRFSSMCEHHLVPFFGVAHVAYLPQNGRVAGLSKLARVVDDVSRRPQLQ
HHHHCEEHHHHCCHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHCCHHHH
ERITTTVAEIMMEKLKPLGVMVIMEAEHMCMTIRGVNKPGTKTITSAVRGAFKNDDKLRS
HHHHHHHHHHHHHHCCCCCEEEEEECCHHHHHEECCCCCCHHHHHHHHHHHCCCHHHHHH
EVLALIKHN
HHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11679669 [H]