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The map label for this gene is aroE

Identifier: 226224527

GI number: 226224527

Start: 1986033

End: 1987319

Strand: Reverse

Name: aroE

Synonym: Lm4b_01940

Alternate gene names: 226224527

Gene position: 1987319-1986033 (Counterclockwise)

Preceding gene: 226224528

Following gene: 226224526

Centisome position: 68.23

GC content: 42.27

Gene sequence:

>1287_bases
ATGAAATTAATTACAAATAAACAAGGGCTGGTTGGCGCAATCACAGTCCCTGGAGATAAATCAATGTCCCATCGCAGCAT
TATGTTCGGGGCTATTGCAGAAGGAAAAACGGTCATTCGTCATTTCTTACGTGCAGATGATTGCCTTGGAACAATTAAGG
CATTTAAAGCATTGGGTGTAAAAATTGAAGAAACCGAAGAAGAAATTATTGTTCACGGAACTGGTTTTGATGGGCTAAAG
CAGGCAGATGGTCCACTTGATATTGGTAACTCGGGAACAACGATTCGTTTAATGATGGGCATTTTAGCTGGCCGAGATTT
TGACACAGTCATTTTAGGGGATGAATCAATTGCGAAACGTCCGATGAACCGTGTCATGCTACCACTGCAACAAATGGGGG
CAAAAATGCATGGCAAAGATGGCTCTGAATTTGCTCCAATTACGATAAATGGGAAGCAATCATTAAAACGCATGGAATAT
CATATGCCAGTTGCTAGTGCACAAGTAAAGAGCGCGATTATTTTTGCAGCTTTACAAGCAGAAGGTGAAACGATTATCCA
TGAAAAAGAAAAGACTCGTGATCATACGGAACACATGATTCGCCAATTTGGCGGTGAAATTGAAATGGACGGCTTAACCA
TTCGCGTCAAAGGTGGCCAAACATTTACCGGGCAAGAAATGACGGTTCCAGGCGATGTTTCCTCTGCTGCGTTCTTTATC
GTAGCTGGCTTAATCACACCCGGCAGTGAAATTGAACTTACGCATGTTGGCTTGAACCCTACTAGAACAGGGATTTTTGA
TGTAGTTGAGCAAATGGGTGGAAGTTTAGTTGTCAAAGATTCTAGCAGAAGTACTGGTAAATTAGCGGGTACGGTTGTAG
TTAAAACTAGCGATTTAAAAGGAACGGAAATCGGTGGCGACATTATTCCTCGTTTAATTGACGAAATTCCAGTTATCGCA
CTTTTAGCGACACAAGCAGAAGGAACGACGATTATTAAAGACGCTGCTGAACTAAAAGTAAAAGAAACAAACCGGATTGA
TGCGGTTGCGACAGAACTAAACAAAATGGGCGCAGATATTACTCCAACCGAAGACGGCTTAATTATTCGCGGGAAAACAC
CACTTCATGCGGCCAATGTAACAAGCTACGGGGATCACCGTATTGGCATGATGCTACAAATTGCAGCACTTCTTGTCGAG
GAAGGCGATGTGGAATTAGAACGCCCAGAAGCAGTTTCTGTTTCTTACCCGACTTTCTTTGAAGATATTCGCTCACTTTT
AAAATAA

Upstream 100 bases:

>100_bases
CAAATTACTTTCCAATCGGATGAAGACAGGGACCGGGCGAAACGTTGCATTGAAACAAGAAGTAACTATACATGCCATTA
CGAATAGGAGAGAAATGACG

Downstream 100 bases:

>100_bases
TCACTTCTACCAGTGCTGTATGCTAATCACATCAGCGCTGGTATTTTTATTTAAGAGAGGCAATCACTTTTTCTTATGAG
TGAAGTAATGGTATAATAGA

Product: 3-phosphoshikimate 1-carboxyvinyltransferase

Products: NA

Alternate protein names: 5-enolpyruvylshikimate-3-phosphate synthase; EPSP synthase; EPSPS [H]

Number of amino acids: Translated: 428; Mature: 428

Protein sequence:

>428_residues
MKLITNKQGLVGAITVPGDKSMSHRSIMFGAIAEGKTVIRHFLRADDCLGTIKAFKALGVKIEETEEEIIVHGTGFDGLK
QADGPLDIGNSGTTIRLMMGILAGRDFDTVILGDESIAKRPMNRVMLPLQQMGAKMHGKDGSEFAPITINGKQSLKRMEY
HMPVASAQVKSAIIFAALQAEGETIIHEKEKTRDHTEHMIRQFGGEIEMDGLTIRVKGGQTFTGQEMTVPGDVSSAAFFI
VAGLITPGSEIELTHVGLNPTRTGIFDVVEQMGGSLVVKDSSRSTGKLAGTVVVKTSDLKGTEIGGDIIPRLIDEIPVIA
LLATQAEGTTIIKDAAELKVKETNRIDAVATELNKMGADITPTEDGLIIRGKTPLHAANVTSYGDHRIGMMLQIAALLVE
EGDVELERPEAVSVSYPTFFEDIRSLLK

Sequences:

>Translated_428_residues
MKLITNKQGLVGAITVPGDKSMSHRSIMFGAIAEGKTVIRHFLRADDCLGTIKAFKALGVKIEETEEEIIVHGTGFDGLK
QADGPLDIGNSGTTIRLMMGILAGRDFDTVILGDESIAKRPMNRVMLPLQQMGAKMHGKDGSEFAPITINGKQSLKRMEY
HMPVASAQVKSAIIFAALQAEGETIIHEKEKTRDHTEHMIRQFGGEIEMDGLTIRVKGGQTFTGQEMTVPGDVSSAAFFI
VAGLITPGSEIELTHVGLNPTRTGIFDVVEQMGGSLVVKDSSRSTGKLAGTVVVKTSDLKGTEIGGDIIPRLIDEIPVIA
LLATQAEGTTIIKDAAELKVKETNRIDAVATELNKMGADITPTEDGLIIRGKTPLHAANVTSYGDHRIGMMLQIAALLVE
EGDVELERPEAVSVSYPTFFEDIRSLLK
>Mature_428_residues
MKLITNKQGLVGAITVPGDKSMSHRSIMFGAIAEGKTVIRHFLRADDCLGTIKAFKALGVKIEETEEEIIVHGTGFDGLK
QADGPLDIGNSGTTIRLMMGILAGRDFDTVILGDESIAKRPMNRVMLPLQQMGAKMHGKDGSEFAPITINGKQSLKRMEY
HMPVASAQVKSAIIFAALQAEGETIIHEKEKTRDHTEHMIRQFGGEIEMDGLTIRVKGGQTFTGQEMTVPGDVSSAAFFI
VAGLITPGSEIELTHVGLNPTRTGIFDVVEQMGGSLVVKDSSRSTGKLAGTVVVKTSDLKGTEIGGDIIPRLIDEIPVIA
LLATQAEGTTIIKDAAELKVKETNRIDAVATELNKMGADITPTEDGLIIRGKTPLHAANVTSYGDHRIGMMLQIAALLVE
EGDVELERPEAVSVSYPTFFEDIRSLLK

Specific function: Aromatic amino acids biosynthesis; shikimate pathway; sixth step. [C]

COG id: COG0128

COG function: function code E; 5-enolpyruvylshikimate-3-phosphate synthase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the EPSP synthase family [H]

Homologues:

Organism=Escherichia coli, GI1787137, Length=427, Percent_Identity=30.4449648711944, Blast_Score=155, Evalue=4e-39,
Organism=Saccharomyces cerevisiae, GI6320332, Length=457, Percent_Identity=25.164113785558, Blast_Score=107, Evalue=2e-24,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001986
- InterPro:   IPR006264
- InterPro:   IPR023193
- InterPro:   IPR013792 [H]

Pfam domain/function: PF00275 EPSP_synthase [H]

EC number: =2.5.1.19 [H]

Molecular weight: Translated: 46027; Mature: 46027

Theoretical pI: Translated: 5.48; Mature: 5.48

Prosite motif: PS00104 EPSP_SYNTHASE_1 ; PS00885 EPSP_SYNTHASE_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.2 %Cys     (Translated Protein)
4.2 %Met     (Translated Protein)
4.4 %Cys+Met (Translated Protein)
0.2 %Cys     (Mature Protein)
4.2 %Met     (Mature Protein)
4.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKLITNKQGLVGAITVPGDKSMSHRSIMFGAIAEGKTVIRHFLRADDCLGTIKAFKALGV
CCCEECCCCCEEEEECCCCCCCCCCEEEEEECCCCHHHHHHHHHHHHHHHHHHHHHHHCC
KIEETEEEIIVHGTGFDGLKQADGPLDIGNSGTTIRLMMGILAGRDFDTVILGDESIAKR
EEECCCCEEEEEECCCCCCCCCCCCEEECCCCCCHHHHHHHHCCCCCCEEEECCHHHHHC
PMNRVMLPLQQMGAKMHGKDGSEFAPITINGKQSLKRMEYHMPVASAQVKSAIIFAALQA
CHHHHHHHHHHCCCEECCCCCCCEEEEEECCHHHHHHHHHHCCHHHHHHHHHEEEEEEEC
EGETIIHEKEKTRDHTEHMIRQFGGEIEMDGLTIRVKGGQTFTGQEMTVPGDVSSAAFFI
CCCEEEECCHHHHHHHHHHHHHHCCCEEECCEEEEEECCCEECCCEEECCCCCCHHHHHH
VAGLITPGSEIELTHVGLNPTRTGIFDVVEQMGGSLVVKDSSRSTGKLAGTVVVKTSDLK
HHHHCCCCCCEEEEEECCCCCHHHHHHHHHHCCCEEEEECCCCCCCCEEEEEEEEECCCC
GTEIGGDIIPRLIDEIPVIALLATQAEGTTIIKDAAELKVKETNRIDAVATELNKMGADI
CCCCCHHHHHHHHHHCCEEEEEEECCCCCEEEECHHHEEEECCCCHHHHHHHHHHCCCCC
TPTEDGLIIRGKTPLHAANVTSYGDHRIGMMLQIAALLVEEGDVELERPEAVSVSYPTFF
CCCCCCEEEECCCCCCCCCCCCCCCCHHHHHHHHHHHHHHCCCCEEECCCEEEEECCHHH
EDIRSLLK
HHHHHHHC
>Mature Secondary Structure
MKLITNKQGLVGAITVPGDKSMSHRSIMFGAIAEGKTVIRHFLRADDCLGTIKAFKALGV
CCCEECCCCCEEEEECCCCCCCCCCEEEEEECCCCHHHHHHHHHHHHHHHHHHHHHHHCC
KIEETEEEIIVHGTGFDGLKQADGPLDIGNSGTTIRLMMGILAGRDFDTVILGDESIAKR
EEECCCCEEEEEECCCCCCCCCCCCEEECCCCCCHHHHHHHHCCCCCCEEEECCHHHHHC
PMNRVMLPLQQMGAKMHGKDGSEFAPITINGKQSLKRMEYHMPVASAQVKSAIIFAALQA
CHHHHHHHHHHCCCEECCCCCCCEEEEEECCHHHHHHHHHHCCHHHHHHHHHEEEEEEEC
EGETIIHEKEKTRDHTEHMIRQFGGEIEMDGLTIRVKGGQTFTGQEMTVPGDVSSAAFFI
CCCEEEECCHHHHHHHHHHHHHHCCCEEECCEEEEEECCCEECCCEEECCCCCCHHHHHH
VAGLITPGSEIELTHVGLNPTRTGIFDVVEQMGGSLVVKDSSRSTGKLAGTVVVKTSDLK
HHHHCCCCCCEEEEEECCCCCHHHHHHHHHHCCCEEEEECCCCCCCCEEEEEEEEECCCC
GTEIGGDIIPRLIDEIPVIALLATQAEGTTIIKDAAELKVKETNRIDAVATELNKMGADI
CCCCCHHHHHHHHHHCCEEEEEEECCCCCEEEECHHHEEEECCCCHHHHHHHHHHCCCCC
TPTEDGLIIRGKTPLHAANVTSYGDHRIGMMLQIAALLVEEGDVELERPEAVSVSYPTFF
CCCCCCEEEECCCCCCCCCCCCCCCCHHHHHHHHHHHHHHCCCCEEECCCEEEEECCHHH
EDIRSLLK
HHHHHHHC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11679669 [H]