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The map label for this gene is pyrC

Identifier: 226224440

GI number: 226224440

Start: 1900162

End: 1901442

Strand: Reverse

Name: pyrC

Synonym: Lm4b_01853

Alternate gene names: 226224440

Gene position: 1901442-1900162 (Counterclockwise)

Preceding gene: 226224441

Following gene: 226224439

Centisome position: 65.28

GC content: 41.92

Gene sequence:

>1281_bases
ATGTACGTATTAAAAAATGGGCAAGTATTAAACGCGTCAGGTGAACTGGAAAACAAAGATGTACTTATTCAAAACGGTAA
AGTCAATTTGATTGCGGATTCCATTGAAGTAACAAGCGGCGAGGAATTTGATGCGACAGGGAAATTAATTGCCCCAGGTT
TTATCGACGTCCATGTACATCTTCGTGAGCCGGGCGGGGAACATAAAGAAACCATTTTAACAGGAACACAAGCGGCGGCA
CGCGGTGGCTATACGACAATTTGTTCGATGCCGAATACAAAACCTGTTCCAGATTCCAAAGAAGTAATGGAAAGTTTACA
AGCAAAAATTAAAGAAACAGCGAAAGTTCGCGTGTTACCATATGCTTCGATTACAACAAGCCTCGGAACGGACGAATTAG
TTGATTTTGAAGCTTTAAAAGAAGCTGGTGCATTTGCTTTTACAGATGACGGGGTAGGCGTGCAGTTAGCTGGAACAATG
TATGAAGCGATGAAACGAGCAGCAGCACTAGATATGGCGATTGTGGCTCACTGTGAAGATAACTCACTTATCTATGGTGG
CGTTGTCCATGACGGGATTTTTGCTGAAAAAGAAGGTCTGAAAGGTATTCCGAATATTGCTGAATCTGTCCAAATCGCAC
GTGACGTTTTACTAGCAGAAGCTGCAGGTTGTCATTATCATGTCTGTCATATTTCAACAAAAGAATCTGTTCGTGTTGTG
CGAGATGCAAAACGGGCGGGAATTCGTGTCACTGCTGAAGTTTCACCACATCACTTAATTTTAGATGAAGAAGCTATTCC
AGGAAATGACGGTAATTGGAAAATGAACCCCCCACTTCGTAGCAAAGAAGATCGGGCGGCACTTTTAGAAGGCTTACTGG
ATGGTACGATAGATTTTATCGCAACTGATCATGCTCCACACGCAGCGGAAGAAAAAAATGTACCGATGGAGCAAGCTGCT
TTCGGAATTGTAGGTCTAGAAACGGCATTCCCATTACTATATACCCATTTTGTTAAAACGAAGGAATGGACATTAAAACA
GCTAATCGACTGGATGACTGTAAAACCAGCGGAATGCTTTAAACTCCCATACGGGAAATTAGAAGAAGGCAGCGTAGCAG
ATATCGTCGTACTTGATTTAGAAAAAGAAGCGAATATTGACCCAGCTACTTTTTATTCAAAAGGAAAAAACACACCATTT
GTAGGAGAAACGTGTATTGGATGGCCAGTAGCAACGTTTTCGGAAGGCACACTTGTATACAATGAGGGGGAAATTAAATG
A

Upstream 100 bases:

>100_bases
GCGAAAAATCACGCATTGTTACGCAAATGACGAATGGCGTTTTTATAAGAATGGCCATTTTGGAAGCTATTTTAAAAGAA
CAGGAAATGAGGGCGAAATT

Downstream 100 bases:

>100_bases
CAAAGCGAATTTTAATGCTAGAAGATGGCAATTATTTTATCGGTGATGCGATTGGTAGTGAAAAAGAAACCATTGGTGAA
GTTGTCTTCAATACTGGAAT

Product: dihydroorotase

Products: NA

Alternate protein names: DHOase [H]

Number of amino acids: Translated: 426; Mature: 426

Protein sequence:

>426_residues
MYVLKNGQVLNASGELENKDVLIQNGKVNLIADSIEVTSGEEFDATGKLIAPGFIDVHVHLREPGGEHKETILTGTQAAA
RGGYTTICSMPNTKPVPDSKEVMESLQAKIKETAKVRVLPYASITTSLGTDELVDFEALKEAGAFAFTDDGVGVQLAGTM
YEAMKRAAALDMAIVAHCEDNSLIYGGVVHDGIFAEKEGLKGIPNIAESVQIARDVLLAEAAGCHYHVCHISTKESVRVV
RDAKRAGIRVTAEVSPHHLILDEEAIPGNDGNWKMNPPLRSKEDRAALLEGLLDGTIDFIATDHAPHAAEEKNVPMEQAA
FGIVGLETAFPLLYTHFVKTKEWTLKQLIDWMTVKPAECFKLPYGKLEEGSVADIVVLDLEKEANIDPATFYSKGKNTPF
VGETCIGWPVATFSEGTLVYNEGEIK

Sequences:

>Translated_426_residues
MYVLKNGQVLNASGELENKDVLIQNGKVNLIADSIEVTSGEEFDATGKLIAPGFIDVHVHLREPGGEHKETILTGTQAAA
RGGYTTICSMPNTKPVPDSKEVMESLQAKIKETAKVRVLPYASITTSLGTDELVDFEALKEAGAFAFTDDGVGVQLAGTM
YEAMKRAAALDMAIVAHCEDNSLIYGGVVHDGIFAEKEGLKGIPNIAESVQIARDVLLAEAAGCHYHVCHISTKESVRVV
RDAKRAGIRVTAEVSPHHLILDEEAIPGNDGNWKMNPPLRSKEDRAALLEGLLDGTIDFIATDHAPHAAEEKNVPMEQAA
FGIVGLETAFPLLYTHFVKTKEWTLKQLIDWMTVKPAECFKLPYGKLEEGSVADIVVLDLEKEANIDPATFYSKGKNTPF
VGETCIGWPVATFSEGTLVYNEGEIK
>Mature_426_residues
MYVLKNGQVLNASGELENKDVLIQNGKVNLIADSIEVTSGEEFDATGKLIAPGFIDVHVHLREPGGEHKETILTGTQAAA
RGGYTTICSMPNTKPVPDSKEVMESLQAKIKETAKVRVLPYASITTSLGTDELVDFEALKEAGAFAFTDDGVGVQLAGTM
YEAMKRAAALDMAIVAHCEDNSLIYGGVVHDGIFAEKEGLKGIPNIAESVQIARDVLLAEAAGCHYHVCHISTKESVRVV
RDAKRAGIRVTAEVSPHHLILDEEAIPGNDGNWKMNPPLRSKEDRAALLEGLLDGTIDFIATDHAPHAAEEKNVPMEQAA
FGIVGLETAFPLLYTHFVKTKEWTLKQLIDWMTVKPAECFKLPYGKLEEGSVADIVVLDLEKEANIDPATFYSKGKNTPF
VGETCIGWPVATFSEGTLVYNEGEIK

Specific function: Involved In The Anaerobic Utilization Of Allantoin. [C]

COG id: COG0044

COG function: function code F; Dihydroorotase and related cyclic amidohydrolases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the DHOase family. Type 2 subfamily [H]

Homologues:

Organism=Homo sapiens, GI18105007, Length=384, Percent_Identity=31.5104166666667, Blast_Score=146, Evalue=4e-35,
Organism=Homo sapiens, GI4503051, Length=459, Percent_Identity=23.9651416122004, Blast_Score=95, Evalue=1e-19,
Organism=Homo sapiens, GI62422571, Length=459, Percent_Identity=23.9651416122004, Blast_Score=94, Evalue=3e-19,
Organism=Homo sapiens, GI4503375, Length=464, Percent_Identity=23.9224137931034, Blast_Score=90, Evalue=4e-18,
Organism=Homo sapiens, GI19923821, Length=446, Percent_Identity=23.542600896861, Blast_Score=82, Evalue=7e-16,
Organism=Homo sapiens, GI4503377, Length=461, Percent_Identity=22.5596529284165, Blast_Score=80, Evalue=3e-15,
Organism=Homo sapiens, GI4503379, Length=453, Percent_Identity=20.9713024282561, Blast_Score=70, Evalue=4e-12,
Organism=Escherichia coli, GI1786722, Length=443, Percent_Identity=28.6681715575621, Blast_Score=163, Evalue=2e-41,
Organism=Escherichia coli, GI87082175, Length=448, Percent_Identity=27.4553571428571, Blast_Score=134, Evalue=1e-32,
Organism=Caenorhabditis elegans, GI193204318, Length=360, Percent_Identity=32.5, Blast_Score=144, Evalue=8e-35,
Organism=Caenorhabditis elegans, GI17539558, Length=450, Percent_Identity=25.3333333333333, Blast_Score=94, Evalue=2e-19,
Organism=Caenorhabditis elegans, GI86575075, Length=446, Percent_Identity=21.7488789237668, Blast_Score=71, Evalue=1e-12,
Organism=Saccharomyces cerevisiae, GI6322218, Length=395, Percent_Identity=28.3544303797468, Blast_Score=120, Evalue=3e-28,
Organism=Drosophila melanogaster, GI24642586, Length=377, Percent_Identity=31.8302387267904, Blast_Score=145, Evalue=5e-35,
Organism=Drosophila melanogaster, GI18859883, Length=414, Percent_Identity=26.0869565217391, Blast_Score=86, Evalue=7e-17,
Organism=Drosophila melanogaster, GI17137462, Length=456, Percent_Identity=22.5877192982456, Blast_Score=75, Evalue=1e-13,
Organism=Drosophila melanogaster, GI221377917, Length=467, Percent_Identity=22.2698072805139, Blast_Score=68, Evalue=1e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR006680
- InterPro:   IPR004722
- InterPro:   IPR002195
- InterPro:   IPR011059 [H]

Pfam domain/function: PF01979 Amidohydro_1 [H]

EC number: =3.5.2.3 [H]

Molecular weight: Translated: 46144; Mature: 46144

Theoretical pI: Translated: 4.71; Mature: 4.71

Prosite motif: PS00482 DIHYDROOROTASE_1 ; PS00483 DIHYDROOROTASE_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.4 %Cys     (Translated Protein)
2.1 %Met     (Translated Protein)
3.5 %Cys+Met (Translated Protein)
1.4 %Cys     (Mature Protein)
2.1 %Met     (Mature Protein)
3.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MYVLKNGQVLNASGELENKDVLIQNGKVNLIADSIEVTSGEEFDATGKLIAPGFIDVHVH
CEEECCCEEEECCCCCCCCCEEEECCEEEEEEECEEECCCCCCCCCCCEECCCEEEEEEE
LREPGGEHKETILTGTQAAARGGYTTICSMPNTKPVPDSKEVMESLQAKIKETAKVRVLP
EECCCCCCCCEEEECCHHHHCCCCEEEECCCCCCCCCCHHHHHHHHHHHHHHCCEEEEEE
YASITTSLGTDELVDFEALKEAGAFAFTDDGVGVQLAGTMYEAMKRAAALDMAIVAHCED
EHHHCCCCCCCHHHHHHHHHHCCCEEEECCCCCEEEHHHHHHHHHHHHHHCEEEEEEECC
NSLIYGGVVHDGIFAEKEGLKGIPNIAESVQIARDVLLAEAAGCHYHVCHISTKESVRVV
CCEEECCEECCCCEECCCCCCCCCHHHHHHHHHHHHHHHHCCCCEEEEEEECCHHHHHHH
RDAKRAGIRVTAEVSPHHLILDEEAIPGNDGNWKMNPPLRSKEDRAALLEGLLDGTIDFI
HHHHHCCEEEEEECCCCEEEEECCCCCCCCCCEEECCCCCCCCHHHHHHHHHHCCCEEEE
ATDHAPHAAEEKNVPMEQAAFGIVGLETAFPLLYTHFVKTKEWTLKQLIDWMTVKPAECF
EECCCCCCCCCCCCCHHHHHCCEEEHHHHHHHHHHHHHCCCHHHHHHHHHHHCCCCHHHH
KLPYGKLEEGSVADIVVLDLEKEANIDPATFYSKGKNTPFVGETCIGWPVATFSEGTLVY
CCCCCCCCCCCEEEEEEEEECCCCCCCHHHHHCCCCCCCCCCCCCCCCCEEECCCCEEEE
NEGEIK
CCCCCC
>Mature Secondary Structure
MYVLKNGQVLNASGELENKDVLIQNGKVNLIADSIEVTSGEEFDATGKLIAPGFIDVHVH
CEEECCCEEEECCCCCCCCCEEEECCEEEEEEECEEECCCCCCCCCCCEECCCEEEEEEE
LREPGGEHKETILTGTQAAARGGYTTICSMPNTKPVPDSKEVMESLQAKIKETAKVRVLP
EECCCCCCCCEEEECCHHHHCCCCEEEECCCCCCCCCCHHHHHHHHHHHHHHCCEEEEEE
YASITTSLGTDELVDFEALKEAGAFAFTDDGVGVQLAGTMYEAMKRAAALDMAIVAHCED
EHHHCCCCCCCHHHHHHHHHHCCCEEEECCCCCEEEHHHHHHHHHHHHHHCEEEEEEECC
NSLIYGGVVHDGIFAEKEGLKGIPNIAESVQIARDVLLAEAAGCHYHVCHISTKESVRVV
CCEEECCEECCCCEECCCCCCCCCHHHHHHHHHHHHHHHHCCCCEEEEEEECCHHHHHHH
RDAKRAGIRVTAEVSPHHLILDEEAIPGNDGNWKMNPPLRSKEDRAALLEGLLDGTIDFI
HHHHHCCEEEEEECCCCEEEEECCCCCCCCCCEEECCCCCCCCHHHHHHHHHHCCCEEEE
ATDHAPHAAEEKNVPMEQAAFGIVGLETAFPLLYTHFVKTKEWTLKQLIDWMTVKPAECF
EECCCCCCCCCCCCCHHHHHCCEEEHHHHHHHHHHHHHCCCHHHHHHHHHHHCCCCHHHH
KLPYGKLEEGSVADIVVLDLEKEANIDPATFYSKGKNTPFVGETCIGWPVATFSEGTLVY
CCCCCCCCCCCEEEEEEEEECCCCCCCHHHHHCCCCCCCCCCCCCCCCCEEECCCCEEEE
NEGEIK
CCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11679669 [H]