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The map label for this gene is pyrB

Identifier: 226224441

GI number: 226224441

Start: 1901430

End: 1902341

Strand: Reverse

Name: pyrB

Synonym: Lm4b_01854

Alternate gene names: 226224441

Gene position: 1902341-1901430 (Counterclockwise)

Preceding gene: 226224442

Following gene: 226224440

Centisome position: 65.31

GC content: 40.35

Gene sequence:

>912_bases
ATGAAAAATTTGTTGTCAATGGAAGCATTAACCGTCCATGAAATCGAGCATTTATTAGAGCAGGCGGCACAGTTTAAACG
CGGGAAAAAAGCGACTTTTAATGAACAGACTTTCGCGGTCAATATGTTTTTTGAACCAAGTACGAGAACACATACGAGTT
TTGAAGTAGCAGAGAAAAAGTTAGGTGTGGAGGTTGTTTCTTTTGATGCAGCGAGCTCTAGCATGACAAAAGGAGAAACG
CTCTACGATACACTGCTCACAATGCAAGCCGTCGGGGTCAATGTCGCAGTGATTAGACACTCAGAAGAAAATTACTATGC
GGGACTCGAAAAATTAGACATTGCTATTGTGAACGGCGGTGATGGATGCGGTGAACATCCGAGTCAATCACTACTTGACT
TATTTACAATAAAAGAACAATTTGGTACTTTTCAGGGTTTGAAAGTAGCGATTGCTGGTGATATTCGTCACAGTCGAGTT
GCCAATTCCAATATGAAAGTGTTAAAAAGACTTGGTGCGGAACTGTTTTTCTCCGGACCGAGAGAATGGTTCGACGAAAG
TTACTTAGCATATGGCACTTACTTATCGGTTGATGAAATTGTGGAAAAAGTGGATGTGTTGATGCTTTTGCGTGTACAAC
ATGAGCGCCATAGTGGAACAGATGAGTTTACGAAAGAAAGCTACCACGAAAAATTTGGATTGACGGAAAATCGGGCAAAG
CAGTTAAAAGAAGATGCAATCATTATGCACCCAAGCCCTGTGAACCGAGATGTGGAAATTGCTGATAGTTTAGTAGAAAG
CGAAAAATCACGCATTGTTACGCAAATGACGAATGGCGTTTTTATAAGAATGGCCATTTTGGAAGCTATTTTAAAAGAAC
AGGAAATGAGGGCGAAATTATGTACGTATTAA

Upstream 100 bases:

>100_bases
CCGAGCACAGATCAGCATAACACATGACAAAAAAACCTCTGCAGACAAAAAATTTACAGAGGTTTTTCTATAAAATGAAG
TAGAAAAGGTGGCGCTTTAA

Downstream 100 bases:

>100_bases
AAAATGGGCAAGTATTAAACGCGTCAGGTGAACTGGAAAACAAAGATGTACTTATTCAAAACGGTAAAGTCAATTTGATT
GCGGATTCCATTGAAGTAAC

Product: aspartate carbamoyltransferase catalytic subunit

Products: NA

Alternate protein names: Aspartate transcarbamylase; ATCase [H]

Number of amino acids: Translated: 303; Mature: 303

Protein sequence:

>303_residues
MKNLLSMEALTVHEIEHLLEQAAQFKRGKKATFNEQTFAVNMFFEPSTRTHTSFEVAEKKLGVEVVSFDAASSSMTKGET
LYDTLLTMQAVGVNVAVIRHSEENYYAGLEKLDIAIVNGGDGCGEHPSQSLLDLFTIKEQFGTFQGLKVAIAGDIRHSRV
ANSNMKVLKRLGAELFFSGPREWFDESYLAYGTYLSVDEIVEKVDVLMLLRVQHERHSGTDEFTKESYHEKFGLTENRAK
QLKEDAIIMHPSPVNRDVEIADSLVESEKSRIVTQMTNGVFIRMAILEAILKEQEMRAKLCTY

Sequences:

>Translated_303_residues
MKNLLSMEALTVHEIEHLLEQAAQFKRGKKATFNEQTFAVNMFFEPSTRTHTSFEVAEKKLGVEVVSFDAASSSMTKGET
LYDTLLTMQAVGVNVAVIRHSEENYYAGLEKLDIAIVNGGDGCGEHPSQSLLDLFTIKEQFGTFQGLKVAIAGDIRHSRV
ANSNMKVLKRLGAELFFSGPREWFDESYLAYGTYLSVDEIVEKVDVLMLLRVQHERHSGTDEFTKESYHEKFGLTENRAK
QLKEDAIIMHPSPVNRDVEIADSLVESEKSRIVTQMTNGVFIRMAILEAILKEQEMRAKLCTY
>Mature_303_residues
MKNLLSMEALTVHEIEHLLEQAAQFKRGKKATFNEQTFAVNMFFEPSTRTHTSFEVAEKKLGVEVVSFDAASSSMTKGET
LYDTLLTMQAVGVNVAVIRHSEENYYAGLEKLDIAIVNGGDGCGEHPSQSLLDLFTIKEQFGTFQGLKVAIAGDIRHSRV
ANSNMKVLKRLGAELFFSGPREWFDESYLAYGTYLSVDEIVEKVDVLMLLRVQHERHSGTDEFTKESYHEKFGLTENRAK
QLKEDAIIMHPSPVNRDVEIADSLVESEKSRIVTQMTNGVFIRMAILEAILKEQEMRAKLCTY

Specific function: Pyrimidine biosynthesis; second step. [C]

COG id: COG0540

COG function: function code F; Aspartate carbamoyltransferase, catalytic chain

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the ATCase/OTCase family [H]

Homologues:

Organism=Homo sapiens, GI18105007, Length=304, Percent_Identity=32.2368421052632, Blast_Score=153, Evalue=2e-37,
Organism=Escherichia coli, GI2367364, Length=309, Percent_Identity=31.7152103559871, Blast_Score=147, Evalue=7e-37,
Organism=Caenorhabditis elegans, GI193204318, Length=322, Percent_Identity=35.4037267080745, Blast_Score=145, Evalue=2e-35,
Organism=Saccharomyces cerevisiae, GI6322331, Length=264, Percent_Identity=35.9848484848485, Blast_Score=144, Evalue=2e-35,
Organism=Drosophila melanogaster, GI24642586, Length=261, Percent_Identity=30.6513409961686, Blast_Score=131, Evalue=5e-31,

Paralogues:

None

Copy number: 2080 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 14328 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR006132
- InterPro:   IPR006130
- InterPro:   IPR006131
- InterPro:   IPR002082 [H]

Pfam domain/function: PF00185 OTCace; PF02729 OTCace_N [H]

EC number: =2.1.3.2 [H]

Molecular weight: Translated: 34183; Mature: 34183

Theoretical pI: Translated: 5.43; Mature: 5.43

Prosite motif: PS00097 CARBAMOYLTRANSFERASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
3.6 %Met     (Translated Protein)
4.3 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
3.6 %Met     (Mature Protein)
4.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKNLLSMEALTVHEIEHLLEQAAQFKRGKKATFNEQTFAVNMFFEPSTRTHTSFEVAEKK
CCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCEEEEEEEECCCCCCCCHHHHHHHH
LGVEVVSFDAASSSMTKGETLYDTLLTMQAVGVNVAVIRHSEENYYAGLEKLDIAIVNGG
HCEEEEEECCCCCCCCCCHHHHHHHHHHHHHCCEEEEEEECCCCHHCCCEEEEEEEEECC
DGCGEHPSQSLLDLFTIKEQFGTFQGLKVAIAGDIRHSRVANSNMKVLKRLGAELFFSGP
CCCCCCCHHHHHHHHHHHHHHCCCCCEEEEEECCCHHHHHCCCHHHHHHHHCHHHEECCC
REWFDESYLAYGTYLSVDEIVEKVDVLMLLRVQHERHSGTDEFTKESYHEKFGLTENRAK
HHHHCCCHHHHCCEECHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHCCCCHHHHH
QLKEDAIIMHPSPVNRDVEIADSLVESEKSRIVTQMTNGVFIRMAILEAILKEQEMRAKL
HHHCCEEEECCCCCCCCHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHH
CTY
CCC
>Mature Secondary Structure
MKNLLSMEALTVHEIEHLLEQAAQFKRGKKATFNEQTFAVNMFFEPSTRTHTSFEVAEKK
CCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCEEEEEEEECCCCCCCCHHHHHHHH
LGVEVVSFDAASSSMTKGETLYDTLLTMQAVGVNVAVIRHSEENYYAGLEKLDIAIVNGG
HCEEEEEECCCCCCCCCCHHHHHHHHHHHHHCCEEEEEEECCCCHHCCCEEEEEEEEECC
DGCGEHPSQSLLDLFTIKEQFGTFQGLKVAIAGDIRHSRVANSNMKVLKRLGAELFFSGP
CCCCCCCHHHHHHHHHHHHHHCCCCCEEEEEECCCHHHHHCCCHHHHHHHHCHHHEECCC
REWFDESYLAYGTYLSVDEIVEKVDVLMLLRVQHERHSGTDEFTKESYHEKFGLTENRAK
HHHHCCCHHHHCCEECHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHCCCCHHHHH
QLKEDAIIMHPSPVNRDVEIADSLVESEKSRIVTQMTNGVFIRMAILEAILKEQEMRAKL
HHHCCEEEECCCCCCCCHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHH
CTY
CCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11679669 [H]