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The map label for this gene is fmt

Identifier: 226224426

GI number: 226224426

Start: 1883833

End: 1884771

Strand: Reverse

Name: fmt

Synonym: Lm4b_01839

Alternate gene names: 226224426

Gene position: 1884771-1883833 (Counterclockwise)

Preceding gene: 226224427

Following gene: 226224425

Centisome position: 64.71

GC content: 41.75

Gene sequence:

>939_bases
ATGACTAAAATTATTTTTATGGGAACACCGGAATTTTCTGTTCCGGTTTTAACGCAATTAGCCAGCACATATGATGTAGT
TGCAGTTGTGACACAGCCAGATCGCCCAGTTGGACGCAAACGAGTTTTGACACCACCGCCAGTAAAAAAAGCTGCTTTAG
AACTAGCTATTCCAGTTTATCAACCAGAAAAATTACGGACATCTAGCGAATTAGAAGAACTAATTGCTCTAGAAGCCGAC
CTACTTGTAACAGCAGCTTACGGACAAATTTTACCAAATAGTTTGCTTGAATCACCAAAGCATGGTGCGATTAATGTGCA
TGCATCTCTTTTACCAGAGTATCGCGGAGGTGCTCCTGTACACTACGCACTGCTTGACGGGAAAACGGAGACAGGCGTTA
CGATTATGTATATGGTAGAGAAATTGGATGCTGGGGATATGATTAGCCAGCGTAAAATTCCCATCACAGATGAAGATAAT
ACTGGCACCATGTTTGATAAATTGAGCAAATTAGGCGCAGAATTATTAATGGACACATTACCCGACTTTTTAGCTGGAAA
AATAACGGCAATCCCACAAGACCCTGAAAAGGTAACCTTTGCACGTAATATTTCTAGAGAACAAGAAAAAATCGACTGGA
CAAAACCAGGACGTACTATCTTTAATCAAATTCGAGGATTGTCTCCTTGGCCAGTGGCTTATACAACACTGGAAGAAAAA
CCATTTAAAATCTGGGAAGCAACTTATGAAGAAACAAAAGAGGACGGCGAACCGGGTGCCATTTTAGCAGATAAAACAAC
GCTTAAAATCGTAGCAGGCGACGGCACGCTCATCGTACCAACAGTAATCCAACCAGCAGGAAAACCGAAGATGGATGTTC
ACTCATTCATGACCGGAGCTGGCAGAAATTTAAGCAAGACGACAAGGTTTGGTGAATAA

Upstream 100 bases:

>100_bases
CTAAAAACACTAATTACCCATTATCAAAAAGACCAACAAAAAGGTTTAACAATAACTATTGACGTACAGCCTTACGTACT
AATGTAAAAGGAGCAAACCA

Downstream 100 bases:

>100_bases
ATGAAAAAACAAAAAACAGTTCGTGCGATTGCATTAGAACTCATCATTAAAATCGAAAATAATCAATCATACAGCCACTT
ATTAATTAATGACGCATTAA

Product: methionyl-tRNA formyltransferase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 312; Mature: 311

Protein sequence:

>312_residues
MTKIIFMGTPEFSVPVLTQLASTYDVVAVVTQPDRPVGRKRVLTPPPVKKAALELAIPVYQPEKLRTSSELEELIALEAD
LLVTAAYGQILPNSLLESPKHGAINVHASLLPEYRGGAPVHYALLDGKTETGVTIMYMVEKLDAGDMISQRKIPITDEDN
TGTMFDKLSKLGAELLMDTLPDFLAGKITAIPQDPEKVTFARNISREQEKIDWTKPGRTIFNQIRGLSPWPVAYTTLEEK
PFKIWEATYEETKEDGEPGAILADKTTLKIVAGDGTLIVPTVIQPAGKPKMDVHSFMTGAGRNLSKTTRFGE

Sequences:

>Translated_312_residues
MTKIIFMGTPEFSVPVLTQLASTYDVVAVVTQPDRPVGRKRVLTPPPVKKAALELAIPVYQPEKLRTSSELEELIALEAD
LLVTAAYGQILPNSLLESPKHGAINVHASLLPEYRGGAPVHYALLDGKTETGVTIMYMVEKLDAGDMISQRKIPITDEDN
TGTMFDKLSKLGAELLMDTLPDFLAGKITAIPQDPEKVTFARNISREQEKIDWTKPGRTIFNQIRGLSPWPVAYTTLEEK
PFKIWEATYEETKEDGEPGAILADKTTLKIVAGDGTLIVPTVIQPAGKPKMDVHSFMTGAGRNLSKTTRFGE
>Mature_311_residues
TKIIFMGTPEFSVPVLTQLASTYDVVAVVTQPDRPVGRKRVLTPPPVKKAALELAIPVYQPEKLRTSSELEELIALEADL
LVTAAYGQILPNSLLESPKHGAINVHASLLPEYRGGAPVHYALLDGKTETGVTIMYMVEKLDAGDMISQRKIPITDEDNT
GTMFDKLSKLGAELLMDTLPDFLAGKITAIPQDPEKVTFARNISREQEKIDWTKPGRTIFNQIRGLSPWPVAYTTLEEKP
FKIWEATYEETKEDGEPGAILADKTTLKIVAGDGTLIVPTVIQPAGKPKMDVHSFMTGAGRNLSKTTRFGE

Specific function: Modifies the free amino group of the aminoacyl moiety of methionyl-tRNA(fMet). The formyl group appears to play a dual role in the initiator identity of N-formylmethionyl-tRNA by:(I) promoting its recognition by IF2 and (II) impairing its binding to EFTu-

COG id: COG0223

COG function: function code J; Methionyl-tRNA formyltransferase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the fmt family [H]

Homologues:

Organism=Homo sapiens, GI21614513, Length=253, Percent_Identity=27.6679841897233, Blast_Score=105, Evalue=4e-23,
Organism=Homo sapiens, GI164663775, Length=236, Percent_Identity=30.0847457627119, Blast_Score=92, Evalue=5e-19,
Organism=Homo sapiens, GI238814322, Length=253, Percent_Identity=26.0869565217391, Blast_Score=92, Evalue=5e-19,
Organism=Escherichia coli, GI1789683, Length=298, Percent_Identity=42.9530201342282, Blast_Score=246, Evalue=9e-67,
Organism=Escherichia coli, GI1788589, Length=282, Percent_Identity=30.8510638297872, Blast_Score=153, Evalue=2e-38,
Organism=Caenorhabditis elegans, GI133930964, Length=264, Percent_Identity=28.030303030303, Blast_Score=94, Evalue=8e-20,
Organism=Saccharomyces cerevisiae, GI6319458, Length=280, Percent_Identity=27.5, Blast_Score=79, Evalue=9e-16,
Organism=Drosophila melanogaster, GI45550868, Length=315, Percent_Identity=29.8412698412698, Blast_Score=118, Evalue=6e-27,
Organism=Drosophila melanogaster, GI28571984, Length=261, Percent_Identity=30.6513409961686, Blast_Score=110, Evalue=1e-24,

Paralogues:

None

Copy number: 400 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR005794
- InterPro:   IPR005793
- InterPro:   IPR002376
- InterPro:   IPR011034
- InterPro:   IPR001555
- InterPro:   IPR015518 [H]

Pfam domain/function: PF02911 Formyl_trans_C; PF00551 Formyl_trans_N [H]

EC number: =2.1.2.9 [H]

Molecular weight: Translated: 34243; Mature: 34112

Theoretical pI: Translated: 5.27; Mature: 5.27

Prosite motif: PS00373 GART

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
2.9 %Met     (Translated Protein)
2.9 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
2.6 %Met     (Mature Protein)
2.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTKIIFMGTPEFSVPVLTQLASTYDVVAVVTQPDRPVGRKRVLTPPPVKKAALELAIPVY
CCEEEEECCCCCCHHHHHHHHCCCEEEEEEECCCCCCCCCCCCCCCCHHHHHHEEEECCC
QPEKLRTSSELEELIALEADLLVTAAYGQILPNSLLESPKHGAINVHASLLPEYRGGAPV
CCHHHCCHHHHHHHHHHHHHHEEEHHHHHHCCHHHHCCCCCCEEEEHHHHCCCCCCCCCE
HYALLDGKTETGVTIMYMVEKLDAGDMISQRKIPITDEDNTGTMFDKLSKLGAELLMDTL
EEEEECCCCCCCEEEEEEEECCCCCCHHHCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHH
PDFLAGKITAIPQDPEKVTFARNISREQEKIDWTKPGRTIFNQIRGLSPWPVAYTTLEEK
HHHHCCCEEECCCCCHHHHHHHHHCHHHHHCCCCCCCHHHHHHHCCCCCCCEEEEECCCC
PFKIWEATYEETKEDGEPGAILADKTTLKIVAGDGTLIVPTVIQPAGKPKMDVHSFMTGA
CHHHHHHHHHHHHCCCCCCEEEECCEEEEEEECCCCEEEEEEECCCCCCCHHHHHHHHCC
GRNLSKTTRFGE
CCCCHHHCCCCC
>Mature Secondary Structure 
TKIIFMGTPEFSVPVLTQLASTYDVVAVVTQPDRPVGRKRVLTPPPVKKAALELAIPVY
CEEEEECCCCCCHHHHHHHHCCCEEEEEEECCCCCCCCCCCCCCCCHHHHHHEEEECCC
QPEKLRTSSELEELIALEADLLVTAAYGQILPNSLLESPKHGAINVHASLLPEYRGGAPV
CCHHHCCHHHHHHHHHHHHHHEEEHHHHHHCCHHHHCCCCCCEEEEHHHHCCCCCCCCCE
HYALLDGKTETGVTIMYMVEKLDAGDMISQRKIPITDEDNTGTMFDKLSKLGAELLMDTL
EEEEECCCCCCCEEEEEEEECCCCCCHHHCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHH
PDFLAGKITAIPQDPEKVTFARNISREQEKIDWTKPGRTIFNQIRGLSPWPVAYTTLEEK
HHHHCCCEEECCCCCHHHHHHHHHCHHHHHCCCCCCCHHHHHHHCCCCCCCEEEEECCCC
PFKIWEATYEETKEDGEPGAILADKTTLKIVAGDGTLIVPTVIQPAGKPKMDVHSFMTGA
CHHHHHHHHHHHHCCCCCCEEEECCEEEEEEECCCCEEEEEEECCCCCCCHHHHHHHHCC
GRNLSKTTRFGE
CCCCHHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11679669 [H]