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The map label for this gene is priA

Identifier: 226224427

GI number: 226224427

Start: 1884785

End: 1887178

Strand: Reverse

Name: priA

Synonym: Lm4b_01840

Alternate gene names: 226224427

Gene position: 1887178-1884785 (Counterclockwise)

Preceding gene: 226224428

Following gene: 226224426

Centisome position: 64.79

GC content: 41.31

Gene sequence:

>2394_bases
ATGATTAATATTGCGAAAGTAATTGTGGACGTTCCAGCCATGCAAGTGGATCGTCCATTTGACTACTATATTCCAGAAGA
TTTAGAAGAACTTATTCGTCCAGGTATGCGAGTAAGTGTCCCATTTGGAAATCGTAAAATACAAGGATTTGTCATTGCTC
TTGGAGAAACAGAAGAAAACCCAAAATTAAAAGGCGTTGACGGGGTGATGGATTTAGCCCCCGTTTTAAATGAAGAATTG
ATGGAACTAGGCGATTGGTTAGCAGAAGATACGCTGAGTTTCCGGGTGTCTGCTTACCAAGCCATGTTACCAGCCGCGCT
ACGAGCAAAATACGAAAAATATTTTTTGCGCTTAGATGAGGAAAACGAAGAATTAGAGCAGCTATTTGAAGGCTACGAAA
CACTAGACTGGAAAGTGGCAGAAGCCCGCGGTTTGCTCAAACAAATTGGCAAATGGGTCAGAGAAGGTAGTGTGGAAGTA
GTCTACCAAGTCAAAAATAAAATCACGAGTAAAAAAGTTCGCGTTGTTAACTGTCTCAAATCACCACACCAACTAGCTGA
AATAATAGAAGATATGCCGAAAAACGCCAAAGCACAGTCACGTGTACTCGCATTTTTCCAAGCTTTTGAAGGCAACGAAA
TTACGGCAGCCGAGTTAAAAAAACAGGCGGAAACAACGGATGCCACAATAAAAAAATTAGTCGACTTAGGGATACTTTCT
ATTCAAGAAAAAATTGTTTCGCGTGATCCCTATGAAAACCACCAATTTGAAAAAAGTGAATCGCTTCAGTTATTACCAGA
TCAACAAACTGCGTGCGAGAAAATCACCGCAGCAACTGATCAAGAAACATTCCTAATACACGGGGTTACTGGAAGTGGGA
AAACCGAAATCTATTTACAAACCATCGAAGCAAAATTAAAAGAGGGTAAAGAAGCCATCGTTTTAGTACCAGAAATTTCC
CTTACCCCACAAATGGTAGAGCGATTTAAAAGCCGCTTTGGAAGCGAGGTCGCGGTACTACATAGTGCGCTATCTTCCGG
AGAAAAATACGACGAATGGCGCAAAATCGAACGCAAAGAAGCGCGCGTTGTCGTTGGGGCTCGTTCCGCCGTATTTGCAC
CGTTTGAAAATCTCGGCATTATTATTATCGACGAAGAGCACGAAGCGAGTTACAAACAAGAAGATAATCCGCGTTATCAT
GCAAGAGATGTAGCCATTTGGCGCGCAACAAAATATCAATGTCCAGTAGTTCTCGGAAGCGCCACGCCGTCCCTTGAATC
TTTTGCTAGAGCGAAAAAAGGTGTGTATACGTTAATCGAACTTCCTAGCCGGGTCAATGATCGGGCGATGCCAGAAGTGA
GCGTTGTCGACATGCGCGAAGAATTACGCAAAGAAAACCGCACCGAATTTTCGACTGAATTACTAGAAAAAATCAAAGAG
CGAATTGCCAAAAAAGAACAAACAGTTTTAATGCTAAATCGCCGAGGTTATTCTTCTTTTGTGATGTGCCGTGATTGTGG
TTATGTTGTGGAATGTCCGAACTGCGATATTTCGCTCACCTATCATCAATCGAGCAATCAAATGAAATGCCATTACTGCG
GACATGAAGAACGTGTTCCGCAAAAATGTCCGAGCTGTGAGGGCGAGCATATTCGTTATTTTGGCACCGGTACACAAAAA
GTAGAAGAAAGTTTGACCAAATTAATTCCAGAAGCGCGCGTCATCCGGATGGACGTGGATACCACTAGAACAAAAGGTGC
TCATGAAAAATTACTCAAAAGCTTCCGCAATCACGAAGCCGATATTTTACTTGGAACACAAATGATTGCCAAAGGACTAG
ATTTTCCAGATATTACGTTAGTTGGCGTATTAAATGCTGACACAATGCTTCATTTACCAGACTTTCGCGCTTCGGAACGA
ACTTTCCAACTTCTCACTCAAGTAAGTGGACGAGCAGGTCGACATGAGCGGACAGGAGAAGTCATCGTTCAAAGCTATAA
CCCAGAACATTACAGTATTGAATTCGCCAAAAAACATGATTTCATCGGTTTTTATAACCACGAAATGCAGCTACGAAAAA
TGGGTTCCTACCCGCCATTTTATTACTTAACCATGATTAATGTCAGCGATGAAAATGAAATGAAAGCGATTCGAACCATT
CAAGAAATGGCTCAGTTTTTACGAGGAAAGCTCGGTCCAGATGCCGTGATTCTTGGCCCTGTTCCTAGCACCATCACGAG
AATTAAAAACAAATATCGCTACCAGTGCATTATTAAATATAAAATCGAACCAAACTTAAAAAAAGAACTAAAAACACTAA
TTACCCATTATCAAAAAGACCAACAAAAAGGTTTAACAATAACTATTGACGTACAGCCTTACGTACTAATGTAA

Upstream 100 bases:

>100_bases
CGGAAAGATGGTTCTAGCAAAGCGTTACCGATACTAGATAAAAAAGAAGTTGCTGAACATATCATCAAAGAAACTGCTAA
CTTTTTAAGGAAGTGAACGA

Downstream 100 bases:

>100_bases
AAGGAGCAAACCAATGACTAAAATTATTTTTATGGGAACACCGGAATTTTCTGTTCCGGTTTTAACGCAATTAGCCAGCA
CATATGATGTAGTTGCAGTT

Product: primosome assembly protein PriA

Products: NA

Alternate protein names: ATP-dependent helicase priA; Replication factor Y [H]

Number of amino acids: Translated: 797; Mature: 797

Protein sequence:

>797_residues
MINIAKVIVDVPAMQVDRPFDYYIPEDLEELIRPGMRVSVPFGNRKIQGFVIALGETEENPKLKGVDGVMDLAPVLNEEL
MELGDWLAEDTLSFRVSAYQAMLPAALRAKYEKYFLRLDEENEELEQLFEGYETLDWKVAEARGLLKQIGKWVREGSVEV
VYQVKNKITSKKVRVVNCLKSPHQLAEIIEDMPKNAKAQSRVLAFFQAFEGNEITAAELKKQAETTDATIKKLVDLGILS
IQEKIVSRDPYENHQFEKSESLQLLPDQQTACEKITAATDQETFLIHGVTGSGKTEIYLQTIEAKLKEGKEAIVLVPEIS
LTPQMVERFKSRFGSEVAVLHSALSSGEKYDEWRKIERKEARVVVGARSAVFAPFENLGIIIIDEEHEASYKQEDNPRYH
ARDVAIWRATKYQCPVVLGSATPSLESFARAKKGVYTLIELPSRVNDRAMPEVSVVDMREELRKENRTEFSTELLEKIKE
RIAKKEQTVLMLNRRGYSSFVMCRDCGYVVECPNCDISLTYHQSSNQMKCHYCGHEERVPQKCPSCEGEHIRYFGTGTQK
VEESLTKLIPEARVIRMDVDTTRTKGAHEKLLKSFRNHEADILLGTQMIAKGLDFPDITLVGVLNADTMLHLPDFRASER
TFQLLTQVSGRAGRHERTGEVIVQSYNPEHYSIEFAKKHDFIGFYNHEMQLRKMGSYPPFYYLTMINVSDENEMKAIRTI
QEMAQFLRGKLGPDAVILGPVPSTITRIKNKYRYQCIIKYKIEPNLKKELKTLITHYQKDQQKGLTITIDVQPYVLM

Sequences:

>Translated_797_residues
MINIAKVIVDVPAMQVDRPFDYYIPEDLEELIRPGMRVSVPFGNRKIQGFVIALGETEENPKLKGVDGVMDLAPVLNEEL
MELGDWLAEDTLSFRVSAYQAMLPAALRAKYEKYFLRLDEENEELEQLFEGYETLDWKVAEARGLLKQIGKWVREGSVEV
VYQVKNKITSKKVRVVNCLKSPHQLAEIIEDMPKNAKAQSRVLAFFQAFEGNEITAAELKKQAETTDATIKKLVDLGILS
IQEKIVSRDPYENHQFEKSESLQLLPDQQTACEKITAATDQETFLIHGVTGSGKTEIYLQTIEAKLKEGKEAIVLVPEIS
LTPQMVERFKSRFGSEVAVLHSALSSGEKYDEWRKIERKEARVVVGARSAVFAPFENLGIIIIDEEHEASYKQEDNPRYH
ARDVAIWRATKYQCPVVLGSATPSLESFARAKKGVYTLIELPSRVNDRAMPEVSVVDMREELRKENRTEFSTELLEKIKE
RIAKKEQTVLMLNRRGYSSFVMCRDCGYVVECPNCDISLTYHQSSNQMKCHYCGHEERVPQKCPSCEGEHIRYFGTGTQK
VEESLTKLIPEARVIRMDVDTTRTKGAHEKLLKSFRNHEADILLGTQMIAKGLDFPDITLVGVLNADTMLHLPDFRASER
TFQLLTQVSGRAGRHERTGEVIVQSYNPEHYSIEFAKKHDFIGFYNHEMQLRKMGSYPPFYYLTMINVSDENEMKAIRTI
QEMAQFLRGKLGPDAVILGPVPSTITRIKNKYRYQCIIKYKIEPNLKKELKTLITHYQKDQQKGLTITIDVQPYVLM
>Mature_797_residues
MINIAKVIVDVPAMQVDRPFDYYIPEDLEELIRPGMRVSVPFGNRKIQGFVIALGETEENPKLKGVDGVMDLAPVLNEEL
MELGDWLAEDTLSFRVSAYQAMLPAALRAKYEKYFLRLDEENEELEQLFEGYETLDWKVAEARGLLKQIGKWVREGSVEV
VYQVKNKITSKKVRVVNCLKSPHQLAEIIEDMPKNAKAQSRVLAFFQAFEGNEITAAELKKQAETTDATIKKLVDLGILS
IQEKIVSRDPYENHQFEKSESLQLLPDQQTACEKITAATDQETFLIHGVTGSGKTEIYLQTIEAKLKEGKEAIVLVPEIS
LTPQMVERFKSRFGSEVAVLHSALSSGEKYDEWRKIERKEARVVVGARSAVFAPFENLGIIIIDEEHEASYKQEDNPRYH
ARDVAIWRATKYQCPVVLGSATPSLESFARAKKGVYTLIELPSRVNDRAMPEVSVVDMREELRKENRTEFSTELLEKIKE
RIAKKEQTVLMLNRRGYSSFVMCRDCGYVVECPNCDISLTYHQSSNQMKCHYCGHEERVPQKCPSCEGEHIRYFGTGTQK
VEESLTKLIPEARVIRMDVDTTRTKGAHEKLLKSFRNHEADILLGTQMIAKGLDFPDITLVGVLNADTMLHLPDFRASER
TFQLLTQVSGRAGRHERTGEVIVQSYNPEHYSIEFAKKHDFIGFYNHEMQLRKMGSYPPFYYLTMINVSDENEMKAIRTI
QEMAQFLRGKLGPDAVILGPVPSTITRIKNKYRYQCIIKYKIEPNLKKELKTLITHYQKDQQKGLTITIDVQPYVLM

Specific function: Recognizes a specific hairpin sequence on phiX ssDNA. This structure is then recognized and bound by proteins priB and priC. Formation of the primosome proceeds with the subsequent actions of dnaB, dnaC, dnaT and primase. PriA then functions as a helicase

COG id: COG1198

COG function: function code L; Primosomal protein N' (replication factor Y) - superfamily II helicase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 helicase C-terminal domain [H]

Homologues:

Organism=Escherichia coli, GI1790370, Length=541, Percent_Identity=38.6321626617375, Blast_Score=426, Evalue=1e-120,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR014001
- InterPro:   IPR011545
- InterPro:   IPR001650
- InterPro:   IPR014021
- InterPro:   IPR005259 [H]

Pfam domain/function: PF00270 DEAD; PF00271 Helicase_C [H]

EC number: NA

Molecular weight: Translated: 91121; Mature: 91121

Theoretical pI: Translated: 6.61; Mature: 6.61

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.5 %Cys     (Translated Protein)
2.8 %Met     (Translated Protein)
4.3 %Cys+Met (Translated Protein)
1.5 %Cys     (Mature Protein)
2.8 %Met     (Mature Protein)
4.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MINIAKVIVDVPAMQVDRPFDYYIPEDLEELIRPGMRVSVPFGNRKIQGFVIALGETEEN
CCCHHHHHHCCCCHHCCCCCCCCCCHHHHHHHCCCCEEEECCCCCEEEEEEEEECCCCCC
PKLKGVDGVMDLAPVLNEELMELGDWLAEDTLSFRVSAYQAMLPAALRAKYEKYFLRLDE
CCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHEEECC
ENEELEQLFEGYETLDWKVAEARGLLKQIGKWVREGSVEVVYQVKNKITSKKVRVVNCLK
CHHHHHHHHHCHHHCCEEHHHHHHHHHHHHHHHHCCCCEEEEHHHHHHHHHHHHHHHHHC
SPHQLAEIIEDMPKNAKAQSRVLAFFQAFEGNEITAAELKKQAETTDATIKKLVDLGILS
CHHHHHHHHHHCCCCCHHHHHHHHHHHHCCCCCEEHHHHHHHHHHHHHHHHHHHHHHHHH
IQEKIVSRDPYENHQFEKSESLQLLPDQQTACEKITAATDQETFLIHGVTGSGKTEIYLQ
HHHHHHCCCCCCCCCCCCCCCCEECCCHHHHHHHHHCCCCCCEEEEEEECCCCCCEEEEH
TIEAKLKEGKEAIVLVPEISLTPQMVERFKSRFGSEVAVLHSALSSGEKYDEWRKIERKE
HHHHHHHCCCCEEEEEECCCCCHHHHHHHHHHHCCHHHHHHHHHHCCCHHHHHHHHHHHC
ARVVVGARSAVFAPFENLGIIIIDEEHEASYKQEDNPRYHARDVAIWRATKYQCPVVLGS
CEEEEECCCCEECCHHHCCEEEEECCCCCCCCCCCCCCEEEEEEEEEEECCCCCCEEECC
ATPSLESFARAKKGVYTLIELPSRVNDRAMPEVSVVDMREELRKENRTEFSTELLEKIKE
CCCCHHHHHHHHCCEEEEEECCCCCCCCCCCCCHHHHHHHHHHHHCCHHHHHHHHHHHHH
RIAKKEQTVLMLNRRGYSSFVMCRDCGYVVECPNCDISLTYHQSSNQMKCHYCGHEERVP
HHHCCCCEEEEEECCCCCCEEEEECCCCEEECCCCCEEEEEECCCCCEEEEECCCCCCCH
QKCPSCEGEHIRYFGTGTQKVEESLTKLIPEARVIRMDVDTTRTKGAHEKLLKSFRNHEA
HHCCCCCCCCEEEECCCHHHHHHHHHHHCCCCEEEEEECCCCCCCCHHHHHHHHHCCCCC
DILLGTQMIAKGLDFPDITLVGVLNADTMLHLPDFRASERTFQLLTQVSGRAGRHERTGE
EEEECHHHHHCCCCCCCEEEEEEECCCCEEECCCCCCHHHHHHHHHHHHCCCCCCCCCCC
VIVQSYNPEHYSIEFAKKHDFIGFYNHEMQLRKMGSYPPFYYLTMINVSDENEMKAIRTI
EEEEECCCCEEEEEEECCCCCEEEECCCHHHHHCCCCCCEEEEEEEEECCCHHHHHHHHH
QEMAQFLRGKLGPDAVILGPVPSTITRIKNKYRYQCIIKYKIEPNLKKELKTLITHYQKD
HHHHHHHCCCCCCCEEEECCCCHHHHHHHHCEEEEEEEEEEECCCHHHHHHHHHHHHHHH
QQKGLTITIDVQPYVLM
HHCCCEEEEECCCEEEC
>Mature Secondary Structure
MINIAKVIVDVPAMQVDRPFDYYIPEDLEELIRPGMRVSVPFGNRKIQGFVIALGETEEN
CCCHHHHHHCCCCHHCCCCCCCCCCHHHHHHHCCCCEEEECCCCCEEEEEEEEECCCCCC
PKLKGVDGVMDLAPVLNEELMELGDWLAEDTLSFRVSAYQAMLPAALRAKYEKYFLRLDE
CCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHEEECC
ENEELEQLFEGYETLDWKVAEARGLLKQIGKWVREGSVEVVYQVKNKITSKKVRVVNCLK
CHHHHHHHHHCHHHCCEEHHHHHHHHHHHHHHHHCCCCEEEEHHHHHHHHHHHHHHHHHC
SPHQLAEIIEDMPKNAKAQSRVLAFFQAFEGNEITAAELKKQAETTDATIKKLVDLGILS
CHHHHHHHHHHCCCCCHHHHHHHHHHHHCCCCCEEHHHHHHHHHHHHHHHHHHHHHHHHH
IQEKIVSRDPYENHQFEKSESLQLLPDQQTACEKITAATDQETFLIHGVTGSGKTEIYLQ
HHHHHHCCCCCCCCCCCCCCCCEECCCHHHHHHHHHCCCCCCEEEEEEECCCCCCEEEEH
TIEAKLKEGKEAIVLVPEISLTPQMVERFKSRFGSEVAVLHSALSSGEKYDEWRKIERKE
HHHHHHHCCCCEEEEEECCCCCHHHHHHHHHHHCCHHHHHHHHHHCCCHHHHHHHHHHHC
ARVVVGARSAVFAPFENLGIIIIDEEHEASYKQEDNPRYHARDVAIWRATKYQCPVVLGS
CEEEEECCCCEECCHHHCCEEEEECCCCCCCCCCCCCCEEEEEEEEEEECCCCCCEEECC
ATPSLESFARAKKGVYTLIELPSRVNDRAMPEVSVVDMREELRKENRTEFSTELLEKIKE
CCCCHHHHHHHHCCEEEEEECCCCCCCCCCCCCHHHHHHHHHHHHCCHHHHHHHHHHHHH
RIAKKEQTVLMLNRRGYSSFVMCRDCGYVVECPNCDISLTYHQSSNQMKCHYCGHEERVP
HHHCCCCEEEEEECCCCCCEEEEECCCCEEECCCCCEEEEEECCCCCEEEEECCCCCCCH
QKCPSCEGEHIRYFGTGTQKVEESLTKLIPEARVIRMDVDTTRTKGAHEKLLKSFRNHEA
HHCCCCCCCCEEEECCCHHHHHHHHHHHCCCCEEEEEECCCCCCCCHHHHHHHHHCCCCC
DILLGTQMIAKGLDFPDITLVGVLNADTMLHLPDFRASERTFQLLTQVSGRAGRHERTGE
EEEECHHHHHCCCCCCCEEEEEEECCCCEEECCCCCCHHHHHHHHHHHHCCCCCCCCCCC
VIVQSYNPEHYSIEFAKKHDFIGFYNHEMQLRKMGSYPPFYYLTMINVSDENEMKAIRTI
EEEEECCCCEEEEEEECCCCCEEEECCCHHHHHCCCCCCEEEEEEEEECCCHHHHHHHHH
QEMAQFLRGKLGPDAVILGPVPSTITRIKNKYRYQCIIKYKIEPNLKKELKTLITHYQKD
HHHHHHHCCCCCCCEEEECCCCHHHHHHHHCEEEEEEEEEEECCCHHHHHHHHHHHHHHH
QQKGLTITIDVQPYVLM
HHCCCEEEEECCCEEEC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 9534248; 9384377; 9086272 [H]