Click here to switch to the map view.

The map label for this gene is rsmB [H]

Identifier: 226224425

GI number: 226224425

Start: 1882498

End: 1883832

Strand: Reverse

Name: rsmB [H]

Synonym: Lm4b_01838

Alternate gene names: 226224425

Gene position: 1883832-1882498 (Counterclockwise)

Preceding gene: 226224426

Following gene: 226224424

Centisome position: 64.68

GC content: 38.5

Gene sequence:

>1335_bases
ATGAAAAAACAAAAAACAGTTCGTGCGATTGCATTAGAACTCATCATTAAAATCGAAAATAATCAATCATACAGCCACTT
ATTAATTAATGACGCATTAAAAAAACAAAAGTTAAATCCGCTTGATAAAGGGCTTTTGACTGAATTAGTATATGGTACAA
CACAACGCAAAATTACACTTGATTACTATTTAGCACCGTTTCTCAATAAAGAACCCGACAATTGGGTGAAAAACTTACTA
AGAATGTCCGTTTATCAATTAACCTTTTTAGATAAAGTTCCTGAGCATGCGATTTTAAATGAAGCTGGTGACATCGCGAA
AGATTTAGGACACCAAGGTGTAACTAAGTTCGTCAACGGCGTATTGCGTAATGTTATTCGAAAAGGCGTACCAAGTATTG
ATGCCATCAAAGACCCTGTGCAAAAAATCGCTGTTGAAACAAGTCTGCCAGAATGGTTAGCAAAAAGGTGGACAGATCAA
TATGGTATCGAAAAACTGCGTGAAATCGGTTTAGCATTCCTTGTCGCGCCACACCAAAGTATCCGCGTCAACCAAACAGA
AATTCCTACTGAACAATTAATCAAAGAACTAAATGACCAAGGAATCACCGTGACACGTAATGAATTTATCGAGGAAGCGT
TACTTGTTGAAAAAGGATCTGTAGCAGAAACAAAAGCATACAAAGACGGTAAATGTAGCATTCAAGACGAAAGCTCAATG
CTCGCAGCCTATGCGCTTCAACTAGAAGACAATTTGACGGTATTAGATGCTTGTGCGGCACCAGGCGGGAAAACAACCCA
TATCGCGGAAAAAATGCACGGCACGGGAATGGTTCATGCACTCGATATCCACGAAAAGAAAACCAAGCTAATTGATCAAG
CCGCCAAACGATTGCAACTACTTAATATTCGTACGGCGCATTTAGACGCAAGAACCGCAAGTACCATGTTTGAACCAGAA
ACATTTGACCGTATTTTAGTGGATGCTCCGTGCTCCGGCTTTGGCGTTCTTCGCAGAAAACCAGACATCAAATATGCGAA
AACAGAAAAAGATATCCACAAATTAGCAGAAATTCAATTAGCGATTTTAGATGATGTTAGCCAATTAGTAAAAGAAAATG
GTATATTAGTTTATAGTACTTGTACCATTGACAAGGAAGAAAATGAAACGGTTCTTCGCGCTTTCTTAGAAAAACATCCA
GAATTCACACTAGAACCTGTAGTACTCCCTGAAAAATTGGCGCAAATCAAGAAGGACGATTTTATTCAACTTTTACCAAC
AGATATTGGAAGCGATGGTTTCTTCGTTTCTAGCCTTAGAAAAGTAAAGTCCTGA

Upstream 100 bases:

>100_bases
AACAGTAATCCAACCAGCAGGAAAACCGAAGATGGATGTTCACTCATTCATGACCGGAGCTGGCAGAAATTTAAGCAAGA
CGACAAGGTTTGGTGAATAA

Downstream 100 bases:

>100_bases
AAAATAGTGAGGTGCTTTTAAAATGCATGCAGAATTTAGAACAGATAGAGGCAGAATAAGACATCATAATGAAGACAACG
GCGGGGTTTTTGAAAATAAA

Product: RNA-binding Sun protein

Products: NA

Alternate protein names: rRNA (cytosine-C(5)-)-methyltransferase rsmB [H]

Number of amino acids: Translated: 444; Mature: 444

Protein sequence:

>444_residues
MKKQKTVRAIALELIIKIENNQSYSHLLINDALKKQKLNPLDKGLLTELVYGTTQRKITLDYYLAPFLNKEPDNWVKNLL
RMSVYQLTFLDKVPEHAILNEAGDIAKDLGHQGVTKFVNGVLRNVIRKGVPSIDAIKDPVQKIAVETSLPEWLAKRWTDQ
YGIEKLREIGLAFLVAPHQSIRVNQTEIPTEQLIKELNDQGITVTRNEFIEEALLVEKGSVAETKAYKDGKCSIQDESSM
LAAYALQLEDNLTVLDACAAPGGKTTHIAEKMHGTGMVHALDIHEKKTKLIDQAAKRLQLLNIRTAHLDARTASTMFEPE
TFDRILVDAPCSGFGVLRRKPDIKYAKTEKDIHKLAEIQLAILDDVSQLVKENGILVYSTCTIDKEENETVLRAFLEKHP
EFTLEPVVLPEKLAQIKKDDFIQLLPTDIGSDGFFVSSLRKVKS

Sequences:

>Translated_444_residues
MKKQKTVRAIALELIIKIENNQSYSHLLINDALKKQKLNPLDKGLLTELVYGTTQRKITLDYYLAPFLNKEPDNWVKNLL
RMSVYQLTFLDKVPEHAILNEAGDIAKDLGHQGVTKFVNGVLRNVIRKGVPSIDAIKDPVQKIAVETSLPEWLAKRWTDQ
YGIEKLREIGLAFLVAPHQSIRVNQTEIPTEQLIKELNDQGITVTRNEFIEEALLVEKGSVAETKAYKDGKCSIQDESSM
LAAYALQLEDNLTVLDACAAPGGKTTHIAEKMHGTGMVHALDIHEKKTKLIDQAAKRLQLLNIRTAHLDARTASTMFEPE
TFDRILVDAPCSGFGVLRRKPDIKYAKTEKDIHKLAEIQLAILDDVSQLVKENGILVYSTCTIDKEENETVLRAFLEKHP
EFTLEPVVLPEKLAQIKKDDFIQLLPTDIGSDGFFVSSLRKVKS
>Mature_444_residues
MKKQKTVRAIALELIIKIENNQSYSHLLINDALKKQKLNPLDKGLLTELVYGTTQRKITLDYYLAPFLNKEPDNWVKNLL
RMSVYQLTFLDKVPEHAILNEAGDIAKDLGHQGVTKFVNGVLRNVIRKGVPSIDAIKDPVQKIAVETSLPEWLAKRWTDQ
YGIEKLREIGLAFLVAPHQSIRVNQTEIPTEQLIKELNDQGITVTRNEFIEEALLVEKGSVAETKAYKDGKCSIQDESSM
LAAYALQLEDNLTVLDACAAPGGKTTHIAEKMHGTGMVHALDIHEKKTKLIDQAAKRLQLLNIRTAHLDARTASTMFEPE
TFDRILVDAPCSGFGVLRRKPDIKYAKTEKDIHKLAEIQLAILDDVSQLVKENGILVYSTCTIDKEENETVLRAFLEKHP
EFTLEPVVLPEKLAQIKKDDFIQLLPTDIGSDGFFVSSLRKVKS

Specific function: Unknown

COG id: COG0144

COG function: function code J; tRNA and rRNA cytosine-C5-methylases

Gene ontology:

Cell location: Cytoplasm (Potential) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the methyltransferase superfamily. RsmB/NOP family [H]

Homologues:

Organism=Homo sapiens, GI76150625, Length=316, Percent_Identity=31.3291139240506, Blast_Score=130, Evalue=2e-30,
Organism=Homo sapiens, GI76150623, Length=316, Percent_Identity=31.3291139240506, Blast_Score=130, Evalue=2e-30,
Organism=Homo sapiens, GI32698918, Length=256, Percent_Identity=33.984375, Blast_Score=120, Evalue=2e-27,
Organism=Homo sapiens, GI301336155, Length=192, Percent_Identity=33.8541666666667, Blast_Score=96, Evalue=7e-20,
Organism=Homo sapiens, GI39995082, Length=192, Percent_Identity=33.8541666666667, Blast_Score=96, Evalue=8e-20,
Organism=Homo sapiens, GI11545785, Length=169, Percent_Identity=33.1360946745562, Blast_Score=87, Evalue=4e-17,
Organism=Homo sapiens, GI8922322, Length=230, Percent_Identity=30.8695652173913, Blast_Score=82, Evalue=1e-15,
Organism=Homo sapiens, GI270288818, Length=230, Percent_Identity=30.8695652173913, Blast_Score=82, Evalue=1e-15,
Organism=Homo sapiens, GI23199998, Length=230, Percent_Identity=30.8695652173913, Blast_Score=82, Evalue=1e-15,
Organism=Homo sapiens, GI270288816, Length=230, Percent_Identity=30.8695652173913, Blast_Score=82, Evalue=1e-15,
Organism=Escherichia coli, GI2367212, Length=448, Percent_Identity=31.4732142857143, Blast_Score=183, Evalue=2e-47,
Organism=Escherichia coli, GI87081985, Length=279, Percent_Identity=32.9749103942652, Blast_Score=148, Evalue=6e-37,
Organism=Caenorhabditis elegans, GI17536757, Length=227, Percent_Identity=34.3612334801762, Blast_Score=116, Evalue=2e-26,
Organism=Caenorhabditis elegans, GI71998419, Length=229, Percent_Identity=31.0043668122271, Blast_Score=89, Evalue=3e-18,
Organism=Caenorhabditis elegans, GI115534021, Length=229, Percent_Identity=31.0043668122271, Blast_Score=89, Evalue=3e-18,
Organism=Caenorhabditis elegans, GI25143471, Length=177, Percent_Identity=35.0282485875706, Blast_Score=87, Evalue=2e-17,
Organism=Saccharomyces cerevisiae, GI6324268, Length=236, Percent_Identity=34.7457627118644, Blast_Score=125, Evalue=2e-29,
Organism=Saccharomyces cerevisiae, GI6319447, Length=183, Percent_Identity=33.879781420765, Blast_Score=98, Evalue=3e-21,
Organism=Drosophila melanogaster, GI22024126, Length=311, Percent_Identity=30.5466237942122, Blast_Score=115, Evalue=5e-26,
Organism=Drosophila melanogaster, GI24668781, Length=235, Percent_Identity=32.3404255319149, Blast_Score=98, Evalue=1e-20,
Organism=Drosophila melanogaster, GI21356513, Length=235, Percent_Identity=32.3404255319149, Blast_Score=98, Evalue=1e-20,
Organism=Drosophila melanogaster, GI21355201, Length=193, Percent_Identity=32.1243523316062, Blast_Score=92, Evalue=9e-19,
Organism=Drosophila melanogaster, GI21356579, Length=174, Percent_Identity=31.6091954022989, Blast_Score=81, Evalue=1e-15,
Organism=Drosophila melanogaster, GI19920494, Length=204, Percent_Identity=31.8627450980392, Blast_Score=72, Evalue=1e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001678
- InterPro:   IPR018314
- InterPro:   IPR006027
- InterPro:   IPR004573 [H]

Pfam domain/function: PF01189 Nol1_Nop2_Fmu; PF01029 NusB [H]

EC number: 2.1.1.-

Molecular weight: Translated: 49938; Mature: 49938

Theoretical pI: Translated: 6.92; Mature: 6.92

Prosite motif: PS01153 NOL1_NOP2_SUN

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
1.4 %Met     (Translated Protein)
2.3 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
1.4 %Met     (Mature Protein)
2.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKKQKTVRAIALELIIKIENNQSYSHLLINDALKKQKLNPLDKGLLTELVYGTTQRKITL
CCCHHHHHHHHHHHHEEECCCCCHHHHHHHHHHHHHCCCHHHHHHHHHHHHCCCCCEEEE
DYYLAPFLNKEPDNWVKNLLRMSVYQLTFLDKVPEHAILNEAGDIAKDLGHQGVTKFVNG
EEEHHHHCCCCCHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHCCCHHHHHHHH
VLRNVIRKGVPSIDAIKDPVQKIAVETSLPEWLAKRWTDQYGIEKLREIGLAFLVAPHQS
HHHHHHHCCCCCHHHHHHHHHHHHHHCCCHHHHHHHHCHHHHHHHHHHCCEEEEECCCCC
IRVNQTEIPTEQLIKELNDQGITVTRNEFIEEALLVEKGSVAETKAYKDGKCSIQDESSM
EECCCCCCCHHHHHHHHCCCCCEEEHHHHHHHHHHHCCCCCHHHHCCCCCCCCCCCHHHH
LAAYALQLEDNLTVLDACAAPGGKTTHIAEKMHGTGMVHALDIHEKKTKLIDQAAKRLQL
HHHHHEEECCCCEEEEHHCCCCCCHHHHHHHHCCCCCEEEEECHHHHHHHHHHHHHHHHH
LNIRTAHLDARTASTMFEPETFDRILVDAPCSGFGVLRRKPDIKYAKTEKDIHKLAEIQL
HHHHHHHCCHHHHHHCCCCHHHHHEEEECCCCCCHHHCCCCCCCHHHHHHHHHHHHHHHH
AILDDVSQLVKENGILVYSTCTIDKEENETVLRAFLEKHPEFTLEPVVLPEKLAQIKKDD
HHHHHHHHHHHHCCEEEEEEEECCCCCCHHHHHHHHHHCCCCCCCCEECHHHHHHCCCCC
FIQLLPTDIGSDGFFVSSLRKVKS
CEEEECCCCCCCCHHHHHHHHHCC
>Mature Secondary Structure
MKKQKTVRAIALELIIKIENNQSYSHLLINDALKKQKLNPLDKGLLTELVYGTTQRKITL
CCCHHHHHHHHHHHHEEECCCCCHHHHHHHHHHHHHCCCHHHHHHHHHHHHCCCCCEEEE
DYYLAPFLNKEPDNWVKNLLRMSVYQLTFLDKVPEHAILNEAGDIAKDLGHQGVTKFVNG
EEEHHHHCCCCCHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHCCCHHHHHHHH
VLRNVIRKGVPSIDAIKDPVQKIAVETSLPEWLAKRWTDQYGIEKLREIGLAFLVAPHQS
HHHHHHHCCCCCHHHHHHHHHHHHHHCCCHHHHHHHHCHHHHHHHHHHCCEEEEECCCCC
IRVNQTEIPTEQLIKELNDQGITVTRNEFIEEALLVEKGSVAETKAYKDGKCSIQDESSM
EECCCCCCCHHHHHHHHCCCCCEEEHHHHHHHHHHHCCCCCHHHHCCCCCCCCCCCHHHH
LAAYALQLEDNLTVLDACAAPGGKTTHIAEKMHGTGMVHALDIHEKKTKLIDQAAKRLQL
HHHHHEEECCCCEEEEHHCCCCCCHHHHHHHHCCCCCEEEEECHHHHHHHHHHHHHHHHH
LNIRTAHLDARTASTMFEPETFDRILVDAPCSGFGVLRRKPDIKYAKTEKDIHKLAEIQL
HHHHHHHCCHHHHHHCCCCHHHHHEEEECCCCCCHHHCCCCCCCHHHHHHHHHHHHHHHH
AILDDVSQLVKENGILVYSTCTIDKEENETVLRAFLEKHPEFTLEPVVLPEKLAQIKKDD
HHHHHHHHHHHHCCEEEEEEEECCCCCCHHHHHHHHHHCCCCCCCCEECHHHHHHCCCCC
FIQLLPTDIGSDGFFVSSLRKVKS
CEEEECCCCCCCCHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 9534248; 9384377; 9086272 [H]