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The map label for this gene is purC

Identifier: 226224375

GI number: 226224375

Start: 1830686

End: 1831399

Strand: Reverse

Name: purC

Synonym: Lm4b_01786

Alternate gene names: 226224375

Gene position: 1831399-1830686 (Counterclockwise)

Preceding gene: 226224376

Following gene: 226224374

Centisome position: 62.88

GC content: 38.66

Gene sequence:

>714_bases
GTGACTAATGAACTGGTTTATGAAGGAAAGGCAAAACGACTTTTTAAAACAGAAGAAGCAGGTGTCTTGCGTGTTGCCTA
TAAGGATGATGCGACAGCGCTAAACGGGGTGCGAAAGGAATCTTTTGCTGGGAAGGGTGAGCTTAATAACCAAATTACAT
CGCTTATTTTTTCTCATTTAGCAGGAGCGGGAATAGAGAGTCATTTTATTCGAGCGATTTCGGAAACGGAGCAATTGGTG
AAAGAAGTATCAATTATTCCACTTGAAGTGGTTGTTCGCAATGTGATGGCTGGGAGTCTTGCGAAACGGCTTGGAAAAGA
AGAAGGCGAGCTAATTCCAAATGCGATAGTAGAATTTTACTATAAAGATGATGCGTTAGATGACCCGTTTATTAATGATG
ACCATGTGCTTTATTTAGAGCTTGCGACAACGAGTGAGATGGATATAATTCGCCAGGCTGCTCGTTCGATTAATAAGGTG
TTACAAGAATTATTCAATCAAATGAATATTACGCTAATTGATTTTAAATTAGAATTTGGTCGGGATGCGACTGGGAATAT
TTTACTAGCAGATGAGATTTCGCCTGATACATGTAGACTTTGGGACAAGGAAACAAATCAAAAGCTCGATAAGGATGTCT
TTCGTCGGAATATTGGCAATTTAACAGATGTGTATACAGAAGTATTGAATAGATTAAAGCAAGTTCAAAACTAG

Upstream 100 bases:

>100_bases
TTGATCGTTTAGGATTATAATTACGGGCAGAATACAACCTAACCTCTTTGATATTTATTTTTTCAAAGAGGTTAAGTAAT
TTATAAGGAGATAAATTATC

Downstream 100 bases:

>100_bases
GAGGCCACAAAATGTATAATGTCAAAGTTTATGTCACTTTAAAGAAAAGCGTATTAGATCCACAAGGTGTTGCAGTAAAA
GATGCAGCGAAAAGCATGGG

Product: phosphoribosylaminoimidazole-succinocarboxamide synthase

Products: NA

Alternate protein names: SAICAR synthetase [H]

Number of amino acids: Translated: 237; Mature: 236

Protein sequence:

>237_residues
MTNELVYEGKAKRLFKTEEAGVLRVAYKDDATALNGVRKESFAGKGELNNQITSLIFSHLAGAGIESHFIRAISETEQLV
KEVSIIPLEVVVRNVMAGSLAKRLGKEEGELIPNAIVEFYYKDDALDDPFINDDHVLYLELATTSEMDIIRQAARSINKV
LQELFNQMNITLIDFKLEFGRDATGNILLADEISPDTCRLWDKETNQKLDKDVFRRNIGNLTDVYTEVLNRLKQVQN

Sequences:

>Translated_237_residues
MTNELVYEGKAKRLFKTEEAGVLRVAYKDDATALNGVRKESFAGKGELNNQITSLIFSHLAGAGIESHFIRAISETEQLV
KEVSIIPLEVVVRNVMAGSLAKRLGKEEGELIPNAIVEFYYKDDALDDPFINDDHVLYLELATTSEMDIIRQAARSINKV
LQELFNQMNITLIDFKLEFGRDATGNILLADEISPDTCRLWDKETNQKLDKDVFRRNIGNLTDVYTEVLNRLKQVQN
>Mature_236_residues
TNELVYEGKAKRLFKTEEAGVLRVAYKDDATALNGVRKESFAGKGELNNQITSLIFSHLAGAGIESHFIRAISETEQLVK
EVSIIPLEVVVRNVMAGSLAKRLGKEEGELIPNAIVEFYYKDDALDDPFINDDHVLYLELATTSEMDIIRQAARSINKVL
QELFNQMNITLIDFKLEFGRDATGNILLADEISPDTCRLWDKETNQKLDKDVFRRNIGNLTDVYTEVLNRLKQVQN

Specific function: De novo purine biosynthesis; seventh step. [C]

COG id: COG0152

COG function: function code F; Phosphoribosylaminoimidazolesuccinocarboxamide (SAICAR) synthase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the SAICAR synthetase family [H]

Homologues:

Organism=Homo sapiens, GI119220557, Length=239, Percent_Identity=35.1464435146443, Blast_Score=130, Evalue=9e-31,
Organism=Homo sapiens, GI5453539, Length=239, Percent_Identity=35.1464435146443, Blast_Score=130, Evalue=9e-31,
Organism=Homo sapiens, GI119220559, Length=246, Percent_Identity=33.739837398374, Blast_Score=120, Evalue=1e-27,
Organism=Escherichia coli, GI1788820, Length=227, Percent_Identity=41.4096916299559, Blast_Score=183, Evalue=8e-48,
Organism=Caenorhabditis elegans, GI17531275, Length=223, Percent_Identity=36.322869955157, Blast_Score=127, Evalue=5e-30,
Organism=Drosophila melanogaster, GI18860083, Length=240, Percent_Identity=30.8333333333333, Blast_Score=114, Evalue=7e-26,
Organism=Drosophila melanogaster, GI24583917, Length=230, Percent_Identity=29.5652173913043, Blast_Score=100, Evalue=2e-21,

Paralogues:

None

Copy number: 1680 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 13,000 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013816
- InterPro:   IPR001636
- InterPro:   IPR018236 [H]

Pfam domain/function: PF01259 SAICAR_synt [H]

EC number: =6.3.2.6 [H]

Molecular weight: Translated: 26796; Mature: 26665

Theoretical pI: Translated: 4.68; Mature: 4.68

Prosite motif: PS01057 SAICAR_SYNTHETASE_1 ; PS01058 SAICAR_SYNTHETASE_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
1.7 %Met     (Translated Protein)
2.1 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
1.3 %Met     (Mature Protein)
1.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTNELVYEGKAKRLFKTEEAGVLRVAYKDDATALNGVRKESFAGKGELNNQITSLIFSHL
CCCCEEECCCHHHHHCCCCCCEEEEEECCCHHHHHHHHHHHCCCCCCHHHHHHHHHHHHH
AGAGIESHFIRAISETEQLVKEVSIIPLEVVVRNVMAGSLAKRLGKEEGELIPNAIVEFY
HCCCHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHH
YKDDALDDPFINDDHVLYLELATTSEMDIIRQAARSINKVLQELFNQMNITLIDFKLEFG
HCCCCCCCCCCCCCCEEEEEECCCCHHHHHHHHHHHHHHHHHHHHHHCCEEEEEEEEECC
RDATGNILLADEISPDTCRLWDKETNQKLDKDVFRRNIGNLTDVYTEVLNRLKQVQN
CCCCCCEEEECCCCCCHHHHCCCHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHCC
>Mature Secondary Structure 
TNELVYEGKAKRLFKTEEAGVLRVAYKDDATALNGVRKESFAGKGELNNQITSLIFSHL
CCCEEECCCHHHHHCCCCCCEEEEEECCCHHHHHHHHHHHCCCCCCHHHHHHHHHHHHH
AGAGIESHFIRAISETEQLVKEVSIIPLEVVVRNVMAGSLAKRLGKEEGELIPNAIVEFY
HCCCHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHH
YKDDALDDPFINDDHVLYLELATTSEMDIIRQAARSINKVLQELFNQMNITLIDFKLEFG
HCCCCCCCCCCCCCCEEEEEECCCCHHHHHHHHHHHHHHHHHHHHHHCCEEEEEEEEECC
RDATGNILLADEISPDTCRLWDKETNQKLDKDVFRRNIGNLTDVYTEVLNRLKQVQN
CCCCCCEEEECCCCCCHHHHCCCHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11679669 [H]