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The map label for this gene is purB

Identifier: 226224376

GI number: 226224376

Start: 1831480

End: 1832772

Strand: Reverse

Name: purB

Synonym: Lm4b_01787

Alternate gene names: 226224376

Gene position: 1832772-1831480 (Counterclockwise)

Preceding gene: 226224377

Following gene: 226224375

Centisome position: 62.92

GC content: 39.98

Gene sequence:

>1293_bases
ATGTTAGAACGTTATACTCGTAAAGAAATGGGCAACATTTGGACAGAACAAAATCGTTATCAGGCTTGGCTAGAAGTGGA
GATTTTGGCTTGTGAAGCATGGGCAGAGCTTGGTGATATTCCGAAAGAAGATGTTGCGAAGATCCGTGCTAATGCAAAGT
TTGATGTGGATCGGATTCATGAGATTGAACTAGAAACGAGACATGATGTTGTGGCATTTACTCGTTCAGTTTCTGAGTCG
CTTGGTGCGGAACGTAAATGGGTTCATTATGGCTTAACTTCAACAGACGTAGTGGATACAGCGAATTCTTATTTACTTAA
ACAAGCGAATGAGATTTTGCGTAAGGATTTGGAAAATTTCATTGCGATTATTGGCGAAAAAGCAAAAGAACATAAATATA
CCGTAACAATGGGACGTACACATGGTGTTCACGCGGAACCTACTACTTTTGGCTTGAAGCTTGCTCTATGGTACGAGGAA
ATGAAACGTAACCTAGAACGTTTTAATTTTGCAGCTGATGGTGTGGAATTCGGGAAAATCTCTGGTGCGGTCGGGACGTA
TGCGAATATTGATCCTTTTGTGGAGGCTTACGTATGTGAAAAATTAGGAACAACTCCTGCGCCGATTTCTACACAAACGT
TGCAACGCGATCGCCATGCAGAATATTTGGCAACACTTGCATTAATTGCAACTTCTGTAGAAAAATTTGCGGTAGAAGTA
CGTGCACTGCAAAAAAGTGAAGTGCGTGAAGTGGAAGAATTCTTTGCAAAAGGACAAAAAGGTTCATCTGCAATGCCGCA
TAAACGTAATCCAATCGGTTCTGAAAATGTAACTGGATTAGCGCGCGTTATTCGCGGTCATATGGTTACTGCTTATGAAA
ATGTGCCACTTTGGCATGAACGTGATATTTCTCATAGCTCAGCGGAACGAATTATTCTGCCAGATTCCACTATTTTACTT
GATTATATTTTGAATCGTTTTGGAAATATTGTGAAAAACTTGACGGTGTTCCCAGAAAATATGAAACGCAATATGGACAG
AACACTGGGACTTATTTATTCACAGCGTGTATTACTGGCACTTATTGATAAAGGTTTAGCTCGTGAGGCGGCGTATGATG
TCGTACAACCAAGAGCAATGGAAGCTTGGGAAAAACAAGTACCTTTCCGTGAATTAGTAGAACAAGATGCAACCATTACT
GAAAATCTATCTGCGGAAGAAATTGCAGATTGCTTTGATTACAACTATCACCTGAAGAATGTCGACTTAATTTTTGATCG
TTTAGGATTATAA

Upstream 100 bases:

>100_bases
CAAAATGGGTCATGTTACTGTGCTGACTGATGAACCAAAAGCGGTTATACAAGATTTAGAAGAAACGCAAATTTGGAAGT
AACTGGAGGAATTTAAAAGG

Downstream 100 bases:

>100_bases
TTACGGGCAGAATACAACCTAACCTCTTTGATATTTATTTTTTCAAAGAGGTTAAGTAATTTATAAGGAGATAAATTATC
GTGACTAATGAACTGGTTTA

Product: adenylosuccinate lyase

Products: NA

Alternate protein names: ASL; Adenylosuccinase; ASase; Glutamyl--tRNA ligase regulatory factor [H]

Number of amino acids: Translated: 430; Mature: 430

Protein sequence:

>430_residues
MLERYTRKEMGNIWTEQNRYQAWLEVEILACEAWAELGDIPKEDVAKIRANAKFDVDRIHEIELETRHDVVAFTRSVSES
LGAERKWVHYGLTSTDVVDTANSYLLKQANEILRKDLENFIAIIGEKAKEHKYTVTMGRTHGVHAEPTTFGLKLALWYEE
MKRNLERFNFAADGVEFGKISGAVGTYANIDPFVEAYVCEKLGTTPAPISTQTLQRDRHAEYLATLALIATSVEKFAVEV
RALQKSEVREVEEFFAKGQKGSSAMPHKRNPIGSENVTGLARVIRGHMVTAYENVPLWHERDISHSSAERIILPDSTILL
DYILNRFGNIVKNLTVFPENMKRNMDRTLGLIYSQRVLLALIDKGLAREAAYDVVQPRAMEAWEKQVPFRELVEQDATIT
ENLSAEEIADCFDYNYHLKNVDLIFDRLGL

Sequences:

>Translated_430_residues
MLERYTRKEMGNIWTEQNRYQAWLEVEILACEAWAELGDIPKEDVAKIRANAKFDVDRIHEIELETRHDVVAFTRSVSES
LGAERKWVHYGLTSTDVVDTANSYLLKQANEILRKDLENFIAIIGEKAKEHKYTVTMGRTHGVHAEPTTFGLKLALWYEE
MKRNLERFNFAADGVEFGKISGAVGTYANIDPFVEAYVCEKLGTTPAPISTQTLQRDRHAEYLATLALIATSVEKFAVEV
RALQKSEVREVEEFFAKGQKGSSAMPHKRNPIGSENVTGLARVIRGHMVTAYENVPLWHERDISHSSAERIILPDSTILL
DYILNRFGNIVKNLTVFPENMKRNMDRTLGLIYSQRVLLALIDKGLAREAAYDVVQPRAMEAWEKQVPFRELVEQDATIT
ENLSAEEIADCFDYNYHLKNVDLIFDRLGL
>Mature_430_residues
MLERYTRKEMGNIWTEQNRYQAWLEVEILACEAWAELGDIPKEDVAKIRANAKFDVDRIHEIELETRHDVVAFTRSVSES
LGAERKWVHYGLTSTDVVDTANSYLLKQANEILRKDLENFIAIIGEKAKEHKYTVTMGRTHGVHAEPTTFGLKLALWYEE
MKRNLERFNFAADGVEFGKISGAVGTYANIDPFVEAYVCEKLGTTPAPISTQTLQRDRHAEYLATLALIATSVEKFAVEV
RALQKSEVREVEEFFAKGQKGSSAMPHKRNPIGSENVTGLARVIRGHMVTAYENVPLWHERDISHSSAERIILPDSTILL
DYILNRFGNIVKNLTVFPENMKRNMDRTLGLIYSQRVLLALIDKGLAREAAYDVVQPRAMEAWEKQVPFRELVEQDATIT
ENLSAEEIADCFDYNYHLKNVDLIFDRLGL

Specific function: Influences the affinity of glutamyl--tRNA ligase for its substrates and increases its thermostability [H]

COG id: COG0015

COG function: function code F; Adenylosuccinate lyase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the lyase 1 family. Adenylosuccinate lyase subfamily [H]

Homologues:

Organism=Homo sapiens, GI4557269, Length=398, Percent_Identity=29.6482412060301, Blast_Score=168, Evalue=1e-41,
Organism=Homo sapiens, GI183227688, Length=400, Percent_Identity=30, Blast_Score=167, Evalue=2e-41,
Organism=Escherichia coli, GI1787376, Length=312, Percent_Identity=28.2051282051282, Blast_Score=85, Evalue=7e-18,
Organism=Caenorhabditis elegans, GI17508577, Length=403, Percent_Identity=27.0471464019851, Blast_Score=135, Evalue=5e-32,
Organism=Caenorhabditis elegans, GI32564234, Length=352, Percent_Identity=27.2727272727273, Blast_Score=108, Evalue=6e-24,
Organism=Saccharomyces cerevisiae, GI6323391, Length=396, Percent_Identity=29.5454545454545, Blast_Score=164, Evalue=4e-41,
Organism=Drosophila melanogaster, GI24647570, Length=397, Percent_Identity=26.7002518891688, Blast_Score=145, Evalue=7e-35,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR019468
- InterPro:   IPR003031
- InterPro:   IPR000362
- InterPro:   IPR020557
- InterPro:   IPR008948
- InterPro:   IPR022761
- InterPro:   IPR004769 [H]

Pfam domain/function: PF10397 ADSL_C; PF00206 Lyase_1 [H]

EC number: =4.3.2.2 [H]

Molecular weight: Translated: 49055; Mature: 49055

Theoretical pI: Translated: 5.52; Mature: 5.52

Prosite motif: PS00163 FUMARATE_LYASES

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
2.1 %Met     (Translated Protein)
2.8 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
2.1 %Met     (Mature Protein)
2.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLERYTRKEMGNIWTEQNRYQAWLEVEILACEAWAELGDIPKEDVAKIRANAKFDVDRIH
CCCHHHHHHHHCCCCCCCCEEEEEEEEEEEHHHHHHHCCCCHHHHHHHHCCCCCCHHHHH
EIELETRHDVVAFTRSVSESLGAERKWVHYGLTSTDVVDTANSYLLKQANEILRKDLENF
EEECHHHHHHHHHHHHHHHHHCCCCCEEEECCCCHHHHHHHHHHHHHHHHHHHHHHHHHH
IAIIGEKAKEHKYTVTMGRTHGVHAEPTTFGLKLALWYEEMKRNLERFNFAADGVEFGKI
HHHHCCHHHCCEEEEEECCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHCHHCCCCCHHHC
SGAVGTYANIDPFVEAYVCEKLGTTPAPISTQTLQRDRHAEYLATLALIATSVEKFAVEV
CCCCCCCCCCHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
RALQKSEVREVEEFFAKGQKGSSAMPHKRNPIGSENVTGLARVIRGHMVTAYENVPLWHE
HHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHEEEECCCCCCCC
RDISHSSAERIILPDSTILLDYILNRFGNIVKNLTVFPENMKRNMDRTLGLIYSQRVLLA
CCCCCCCCCEEECCCHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHH
LIDKGLAREAAYDVVQPRAMEAWEKQVPFRELVEQDATITENLSAEEIADCFDYNYHLKN
HHHHHHHHHHHHHHHCCHHHHHHHHCCCHHHHHHCCCHHHCCCCHHHHHHHHCCCCEECH
VDLIFDRLGL
HHHHHHHHCC
>Mature Secondary Structure
MLERYTRKEMGNIWTEQNRYQAWLEVEILACEAWAELGDIPKEDVAKIRANAKFDVDRIH
CCCHHHHHHHHCCCCCCCCEEEEEEEEEEEHHHHHHHCCCCHHHHHHHHCCCCCCHHHHH
EIELETRHDVVAFTRSVSESLGAERKWVHYGLTSTDVVDTANSYLLKQANEILRKDLENF
EEECHHHHHHHHHHHHHHHHHCCCCCEEEECCCCHHHHHHHHHHHHHHHHHHHHHHHHHH
IAIIGEKAKEHKYTVTMGRTHGVHAEPTTFGLKLALWYEEMKRNLERFNFAADGVEFGKI
HHHHCCHHHCCEEEEEECCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHCHHCCCCCHHHC
SGAVGTYANIDPFVEAYVCEKLGTTPAPISTQTLQRDRHAEYLATLALIATSVEKFAVEV
CCCCCCCCCCHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
RALQKSEVREVEEFFAKGQKGSSAMPHKRNPIGSENVTGLARVIRGHMVTAYENVPLWHE
HHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHEEEECCCCCCCC
RDISHSSAERIILPDSTILLDYILNRFGNIVKNLTVFPENMKRNMDRTLGLIYSQRVLLA
CCCCCCCCCEEECCCHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHH
LIDKGLAREAAYDVVQPRAMEAWEKQVPFRELVEQDATITENLSAEEIADCFDYNYHLKN
HHHHHHHHHHHHHHHCCHHHHHHHHCCCHHHHHHCCCHHHCCCCHHHHHHHHCCCCEECH
VDLIFDRLGL
HHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 3036807; 9384377; 1608947; 10926519 [H]