Click here to switch to the map view.

The map label for this gene is purN

Identifier: 226224369

GI number: 226224369

Start: 1824487

End: 1825053

Strand: Reverse

Name: purN

Synonym: Lm4b_01780

Alternate gene names: 226224369

Gene position: 1825053-1824487 (Counterclockwise)

Preceding gene: 226224370

Following gene: 226224368

Centisome position: 62.66

GC content: 38.45

Gene sequence:

>567_bases
ATGAATATAGCCATTTTTGCTTCGGGTAACGGTTCGAATTTTCAAGCTTTAGTGGATGATGCGTTTATTAAGCCTCATGT
GAAACTACTGGTATGTGATAAACCAAATGCTTATGTTTTAGAACGCGCAAACACATATCAGATTCCGGTGTTCCTTTTTG
AAGCGAAAAATTATCCGGATAAAGAAGCTTTTGAGACAGAAATTTTACTAGAACTTCGCGGGTTGGAAATTGATTTGTTA
GTGCTAGCAGGATATATGCGTTTGATTGGGCCGACTTTATTAGCAGAATTTCCCGAGCAAATTGTCAACCTGCACCCATC
GTTGTTACCTGAATTCAAAGGAAAAGATGCAATTGGTCAAGCGATTCAAGCCAATGTTTCAGAAACTGGTGTCACGGCGC
ACTTTGTGGATGCGGGAATGGATACTGGACCAATTATTGATCAAGTGAAAGTACCGATTGAACATGCGGAAACTGTAGAT
ACTTTAGCTGGAAAAATTCATCAAGTAGAACATATTTTTTATCCAAAAGTGATTCGAGGATTAATTCAAAATGGAGGGAA
CGATTAA

Upstream 100 bases:

>100_bases
CATTAGAAGTACTCGTTCAAAACGGGGAAGCGGCTTATGTTATTGGAGAAGTTACGACACGTGAAAATGATGCGGTTATT
TTCACAGGAGGCACAAAAGG

Downstream 100 bases:

>100_bases
TTATCATGAAAAGAGCGCTTATTAGTGTGTCAGATAAAAACGGCATCGTGCCATTTGCAGAAAAATTAGTAGAACTTGGA
GTAGAAATTATTTCGACAGG

Product: phosphoribosylglycinamide formyltransferase

Products: NA

Alternate protein names: 5'-phosphoribosylglycinamide transformylase; GAR transformylase; GART [H]

Number of amino acids: Translated: 188; Mature: 188

Protein sequence:

>188_residues
MNIAIFASGNGSNFQALVDDAFIKPHVKLLVCDKPNAYVLERANTYQIPVFLFEAKNYPDKEAFETEILLELRGLEIDLL
VLAGYMRLIGPTLLAEFPEQIVNLHPSLLPEFKGKDAIGQAIQANVSETGVTAHFVDAGMDTGPIIDQVKVPIEHAETVD
TLAGKIHQVEHIFYPKVIRGLIQNGGND

Sequences:

>Translated_188_residues
MNIAIFASGNGSNFQALVDDAFIKPHVKLLVCDKPNAYVLERANTYQIPVFLFEAKNYPDKEAFETEILLELRGLEIDLL
VLAGYMRLIGPTLLAEFPEQIVNLHPSLLPEFKGKDAIGQAIQANVSETGVTAHFVDAGMDTGPIIDQVKVPIEHAETVD
TLAGKIHQVEHIFYPKVIRGLIQNGGND
>Mature_188_residues
MNIAIFASGNGSNFQALVDDAFIKPHVKLLVCDKPNAYVLERANTYQIPVFLFEAKNYPDKEAFETEILLELRGLEIDLL
VLAGYMRLIGPTLLAEFPEQIVNLHPSLLPEFKGKDAIGQAIQANVSETGVTAHFVDAGMDTGPIIDQVKVPIEHAETVD
TLAGKIHQVEHIFYPKVIRGLIQNGGND

Specific function: De novo purine biosynthesis; third step. [C]

COG id: COG0299

COG function: function code F; Folate-dependent phosphoribosylglycinamide formyltransferase PurN

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the GART family [H]

Homologues:

Organism=Homo sapiens, GI4503915, Length=193, Percent_Identity=34.1968911917098, Blast_Score=122, Evalue=3e-28,
Organism=Homo sapiens, GI209869995, Length=193, Percent_Identity=34.1968911917098, Blast_Score=122, Evalue=3e-28,
Organism=Homo sapiens, GI209869993, Length=193, Percent_Identity=34.1968911917098, Blast_Score=122, Evalue=3e-28,
Organism=Escherichia coli, GI1788846, Length=181, Percent_Identity=35.9116022099448, Blast_Score=127, Evalue=4e-31,
Organism=Escherichia coli, GI1787483, Length=93, Percent_Identity=39.7849462365591, Blast_Score=75, Evalue=3e-15,
Organism=Caenorhabditis elegans, GI17567511, Length=181, Percent_Identity=36.4640883977901, Blast_Score=115, Evalue=1e-26,
Organism=Drosophila melanogaster, GI24582400, Length=186, Percent_Identity=36.5591397849462, Blast_Score=135, Evalue=1e-32,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002376
- InterPro:   IPR001555
- InterPro:   IPR004607 [H]

Pfam domain/function: PF00551 Formyl_trans_N [H]

EC number: =2.1.2.2 [H]

Molecular weight: Translated: 20678; Mature: 20678

Theoretical pI: Translated: 4.62; Mature: 4.62

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
1.6 %Met     (Translated Protein)
2.1 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
1.6 %Met     (Mature Protein)
2.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNIAIFASGNGSNFQALVDDAFIKPHVKLLVCDKPNAYVLERANTYQIPVFLFEAKNYPD
CEEEEEECCCCCCHHHHHHHHHCCCCEEEEEECCCCCEEEECCCCEEEEEEEEECCCCCC
KEAFETEILLELRGLEIDLLVLAGYMRLIGPTLLAEFPEQIVNLHPSLLPEFKGKDAIGQ
HHHHHHHHEEEECCCEEHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHCCCCCCCHHHHH
AIQANVSETGVTAHFVDAGMDTGPIIDQVKVPIEHAETVDTLAGKIHQVEHIFYPKVIRG
HHHCCCCCCCCEEEEEECCCCCCCCHHHEECCHHHHHHHHHHHHHHHHHHHHHHHHHHHH
LIQNGGND
HHHCCCCC
>Mature Secondary Structure
MNIAIFASGNGSNFQALVDDAFIKPHVKLLVCDKPNAYVLERANTYQIPVFLFEAKNYPD
CEEEEEECCCCCCHHHHHHHHHCCCCEEEEEECCCCCEEEECCCCEEEEEEEEECCCCCC
KEAFETEILLELRGLEIDLLVLAGYMRLIGPTLLAEFPEQIVNLHPSLLPEFKGKDAIGQ
HHHHHHHHEEEECCCEEHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHCCCCCCCHHHHH
AIQANVSETGVTAHFVDAGMDTGPIIDQVKVPIEHAETVDTLAGKIHQVEHIFYPKVIRG
HHHCCCCCCCCEEEEEECCCCCCCCHHHEECCHHHHHHHHHHHHHHHHHHHHHHHHHHHH
LIQNGGND
HHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 3036807; 9384377 [H]