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The map label for this gene is purM

Identifier: 226224370

GI number: 226224370

Start: 1825050

End: 1826099

Strand: Reverse

Name: purM

Synonym: Lm4b_01781

Alternate gene names: 226224370

Gene position: 1826099-1825050 (Counterclockwise)

Preceding gene: 226224371

Following gene: 226224369

Centisome position: 62.69

GC content: 42.67

Gene sequence:

>1050_bases
ATGGCAGAAAATGCATATAGTAAAGCAGGCGTGGACGTAGAAGCTGGCTATCAAGTTGTAGAACGTATCAAAAAACATGT
GGCTAGAACAGAGCGATTAGGCGCGATGGGGGCACTTGGTTCATTTGGTGGAATGTTTGACTTAAGTAGTTTGCATTTAA
AAGAACCTGTCCTTGTTTCTGGTACGGATGGTGTTGGGACAAAATTACTTTTGGCAATTGAAGCAGATAAGCATGATACC
ATCGGGATTGACTGCGTGGCAATGTGTGTGAATGACATTTTAGCTCAAGGCGCCGAGCCATTATTTTTCTTAGATTATAT
CGCTACTGGAAAAACAGATCCAGTGAAAATGGAACAAATTGTTAAAGGCGTGGCTGACGGCTGTGAACAAGCTGGTGCGG
CTTTAATTGGCGGCGAAACTGCTGAAATGCCTGATATGTACGGCGCGGAAGATTATGATTTAGCTGGTTTTACTGTTGGG
GCTGTAGAGAAACAGAAGCTAATTACAGAAGGCGCAGTCAAAGAAGGAGATACACTCATTGGTATTCCATCAAGCGGAAT
TCATAGTAATGGTTACTCCCTTGTGCGTAAAATTTTCTTTAAAGATAACGAATTGACACTCGATGCGGAAATCAGTGAAC
TAGATGTACCGCTTGTGGAAGAGCTACTTAAACCGACACGGATTTATGTGAAGCCAGTTTTAGAAGTATTGAAAGAGGTA
GATGTGCACGGAATTACGCATGTGACTGGTGGCGGATTTGTAGAGAATTTACCGCGGATGTTAACAAATGATTTAGCTGT
CAAAGTGGAGCTTGGTTCGTGGCCGGTGTTGCCGATTTTTGACGTTATGAAGAAATACGGCCAATTGAGCGAACTTGAAA
TGTATGAAATTTTCAATATGGGCATTGGTATGGTGTTGGCAGTGGCGAAAGCGGATGTGGAAAGAACATTAGAAGTACTC
GTTCAAAACGGGGAAGCGGCTTATGTTATTGGAGAAGTTACGACACGTGAAAATGATGCGGTTATTTTCACAGGAGGCAC
AAAAGGATGA

Upstream 100 bases:

>100_bases
GGCTTATTTCAACGGCGATTACCCAACCCCTTTATATGATTATGAAGCGGAATATTTAGCGAGCTTAGAAGCAGAAAAAT
AATGAGGAGTGACAAATCGA

Downstream 100 bases:

>100_bases
ATATAGCCATTTTTGCTTCGGGTAACGGTTCGAATTTTCAAGCTTTAGTGGATGATGCGTTTATTAAGCCTCATGTGAAA
CTACTGGTATGTGATAAACC

Product: phosphoribosylaminoimidazole synthetase

Products: NA

Alternate protein names: AIR synthase; AIRS; Phosphoribosyl-aminoimidazole synthetase

Number of amino acids: Translated: 349; Mature: 348

Protein sequence:

>349_residues
MAENAYSKAGVDVEAGYQVVERIKKHVARTERLGAMGALGSFGGMFDLSSLHLKEPVLVSGTDGVGTKLLLAIEADKHDT
IGIDCVAMCVNDILAQGAEPLFFLDYIATGKTDPVKMEQIVKGVADGCEQAGAALIGGETAEMPDMYGAEDYDLAGFTVG
AVEKQKLITEGAVKEGDTLIGIPSSGIHSNGYSLVRKIFFKDNELTLDAEISELDVPLVEELLKPTRIYVKPVLEVLKEV
DVHGITHVTGGGFVENLPRMLTNDLAVKVELGSWPVLPIFDVMKKYGQLSELEMYEIFNMGIGMVLAVAKADVERTLEVL
VQNGEAAYVIGEVTTRENDAVIFTGGTKG

Sequences:

>Translated_349_residues
MAENAYSKAGVDVEAGYQVVERIKKHVARTERLGAMGALGSFGGMFDLSSLHLKEPVLVSGTDGVGTKLLLAIEADKHDT
IGIDCVAMCVNDILAQGAEPLFFLDYIATGKTDPVKMEQIVKGVADGCEQAGAALIGGETAEMPDMYGAEDYDLAGFTVG
AVEKQKLITEGAVKEGDTLIGIPSSGIHSNGYSLVRKIFFKDNELTLDAEISELDVPLVEELLKPTRIYVKPVLEVLKEV
DVHGITHVTGGGFVENLPRMLTNDLAVKVELGSWPVLPIFDVMKKYGQLSELEMYEIFNMGIGMVLAVAKADVERTLEVL
VQNGEAAYVIGEVTTRENDAVIFTGGTKG
>Mature_348_residues
AENAYSKAGVDVEAGYQVVERIKKHVARTERLGAMGALGSFGGMFDLSSLHLKEPVLVSGTDGVGTKLLLAIEADKHDTI
GIDCVAMCVNDILAQGAEPLFFLDYIATGKTDPVKMEQIVKGVADGCEQAGAALIGGETAEMPDMYGAEDYDLAGFTVGA
VEKQKLITEGAVKEGDTLIGIPSSGIHSNGYSLVRKIFFKDNELTLDAEISELDVPLVEELLKPTRIYVKPVLEVLKEVD
VHGITHVTGGGFVENLPRMLTNDLAVKVELGSWPVLPIFDVMKKYGQLSELEMYEIFNMGIGMVLAVAKADVERTLEVLV
QNGEAAYVIGEVTTRENDAVIFTGGTKG

Specific function: De novo purine biosynthesis; fifth step. [C]

COG id: COG0150

COG function: function code F; Phosphoribosylaminoimidazole (AIR) synthetase

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the AIR synthase family

Homologues:

Organism=Homo sapiens, GI4503915, Length=333, Percent_Identity=50.1501501501502, Blast_Score=315, Evalue=4e-86,
Organism=Homo sapiens, GI209869995, Length=333, Percent_Identity=50.1501501501502, Blast_Score=315, Evalue=4e-86,
Organism=Homo sapiens, GI209869993, Length=333, Percent_Identity=50.1501501501502, Blast_Score=315, Evalue=4e-86,
Organism=Escherichia coli, GI1788845, Length=337, Percent_Identity=51.6320474777448, Blast_Score=340, Evalue=1e-94,
Organism=Caenorhabditis elegans, GI17567511, Length=350, Percent_Identity=38, Blast_Score=223, Evalue=1e-58,
Organism=Saccharomyces cerevisiae, GI6321203, Length=350, Percent_Identity=43.4285714285714, Blast_Score=276, Evalue=3e-75,
Organism=Drosophila melanogaster, GI24582400, Length=293, Percent_Identity=43.3447098976109, Blast_Score=222, Evalue=3e-58,

Paralogues:

None

Copy number: 180 Molecules/Cell In: Growth-Phase, Minimal-Media (Based on E. coli). [C]

Swissprot (AC and ID): PUR5_LISMC (C1KW66)

Other databases:

- EMBL:   FM242711
- RefSeq:   YP_002758477.1
- ProteinModelPortal:   C1KW66
- SMR:   C1KW66
- GeneID:   7704088
- GenomeReviews:   FM242711_GR
- KEGG:   lmc:Lm4b_01781
- OMA:   DMYGADD
- ProtClustDB:   PRK05385
- GO:   GO:0005737
- HAMAP:   MF_00741_B
- InterPro:   IPR000728
- InterPro:   IPR010918
- InterPro:   IPR004733
- InterPro:   IPR016188
- TIGRFAMs:   TIGR00878

Pfam domain/function: PF00586 AIRS; PF02769 AIRS_C; SSF56042 AIR_synth_C; SSF55326 PurM_N-like

EC number: =6.3.3.1

Molecular weight: Translated: 37416; Mature: 37284

Theoretical pI: Translated: 4.33; Mature: 4.33

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
3.4 %Met     (Translated Protein)
4.3 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
3.2 %Met     (Mature Protein)
4.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAENAYSKAGVDVEAGYQVVERIKKHVARTERLGAMGALGSFGGMFDLSSLHLKEPVLVS
CCCCCCHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCHHCCCCEEEE
GTDGVGTKLLLAIEADKHDTIGIDCVAMCVNDILAQGAEPLFFLDYIATGKTDPVKMEQI
CCCCCCCEEEEEEECCCCCCCCHHHHHHHHHHHHHCCCCCEEEEEEHHCCCCCCHHHHHH
VKGVADGCEQAGAALIGGETAEMPDMYGAEDYDLAGFTVGAVEKQKLITEGAVKEGDTLI
HHHHHHHHHHCCCEEECCCCCCCCCCCCCCCCCCCCEEECCHHHHHHHHHCCCCCCCEEE
GIPSSGIHSNGYSLVRKIFFKDNELTLDAEISELDVPLVEELLKPTRIYVKPVLEVLKEV
ECCCCCCCCCHHHHHHHHHHCCCCEEEECCHHHCCCHHHHHHHCHHHHHHHHHHHHHHHC
DVHGITHVTGGGFVENLPRMLTNDLAVKVELGSWPVLPIFDVMKKYGQLSELEMYEIFNM
CCCCEEEECCCHHHHHHHHHHCCCEEEEEECCCCCCHHHHHHHHHHCCHHHHHHHHHHHC
GIGMVLAVAKADVERTLEVLVQNGEAAYVIGEVTTRENDAVIFTGGTKG
CHHHHHHHHHHHHHHHHHHHHHCCCEEEEEEEEEECCCCEEEEECCCCC
>Mature Secondary Structure 
AENAYSKAGVDVEAGYQVVERIKKHVARTERLGAMGALGSFGGMFDLSSLHLKEPVLVS
CCCCCHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCHHCCCCEEEE
GTDGVGTKLLLAIEADKHDTIGIDCVAMCVNDILAQGAEPLFFLDYIATGKTDPVKMEQI
CCCCCCCEEEEEEECCCCCCCCHHHHHHHHHHHHHCCCCCEEEEEEHHCCCCCCHHHHHH
VKGVADGCEQAGAALIGGETAEMPDMYGAEDYDLAGFTVGAVEKQKLITEGAVKEGDTLI
HHHHHHHHHHCCCEEECCCCCCCCCCCCCCCCCCCCEEECCHHHHHHHHHCCCCCCCEEE
GIPSSGIHSNGYSLVRKIFFKDNELTLDAEISELDVPLVEELLKPTRIYVKPVLEVLKEV
ECCCCCCCCCHHHHHHHHHHCCCCEEEECCHHHCCCHHHHHHHCHHHHHHHHHHHHHHHC
DVHGITHVTGGGFVENLPRMLTNDLAVKVELGSWPVLPIFDVMKKYGQLSELEMYEIFNM
CCCCEEEECCCHHHHHHHHHHCCCEEEEEECCCCCCHHHHHHHHHHCCHHHHHHHHHHHC
GIGMVLAVAKADVERTLEVLVQNGEAAYVIGEVTTRENDAVIFTGGTKG
CHHHHHHHHHHHHHHHHHHHHHCCCEEEEEEEEEECCCCEEEEECCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA