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The map label for this gene is purH

Identifier: 226224368

GI number: 226224368

Start: 1822952

End: 1824481

Strand: Reverse

Name: purH

Synonym: Lm4b_01779

Alternate gene names: 226224368

Gene position: 1824481-1822952 (Counterclockwise)

Preceding gene: 226224369

Following gene: 226224367

Centisome position: 62.64

GC content: 40.26

Gene sequence:

>1530_bases
ATGAAAAGAGCGCTTATTAGTGTGTCAGATAAAAACGGCATCGTGCCATTTGCAGAAAAATTAGTAGAACTTGGAGTAGA
AATTATTTCGACAGGTGGAACAAAAGCGGCATTTGAACAAGCTGGCGTACCGGTAACAGGAATAGAAGATGTAACTGAAT
TTCCAGAAATGCTTGATGGTCGCGTGAAAACACTTCATCCAGCAATTCATGGTGGGTTACTGGCAAGACGCGATACAGCT
GAACATATGGAAGCTATTGCGGCACACGATATCAAGCCAATTGATTTAGTCGTTGTAAATTTATATCCTTTCCAAGAAAC
GATTCAAAAATCTGGTGTTACTTTAGAAGAAGCGATTGAAAATATTGATATTGGCGGACCTTCGATGTTACGCTCAGCTG
CGAAAAATTATGCAGCGGTAACTGTTGTAGTTGATACAGCGGATTACGATACGGTACTTACAGAACTAGAAGAACACGGC
GCAACTACTTTTGAAACGCGCCAACGTCTAGCTGCGAAAGTTTTTCGGCACACAGCGGCCTATGATGCTTTAATTGCAGA
ATACTTAACGAACATCACTGGAGAAACTTTCCCAGAAAAAGTAACATTAACTTACAATCGAAAACAAGTTTTGCGTTATG
GTGAAAATCCTCACCAAGATGCCGCTTTTTATACAGAACCTGGCACGGTAGAAAATTCAATTAGTTCCGCGAAACAGTTG
CACGGTAAAGAGTTATCTTACAACAATATTCGGGATGCAGATGCAGCACTTAAAATTGCAAGTGAATTTACAGAGCCGGT
CGCGGTTGCAGTAAAACATATGAATCCATGCGGCGTTGGTGTTGGAGAAAATATTGAAGAAGCTTATTTGAAAGCTTATG
AAGCGGATGAAACGTCTATTTTTGGAGGGATTGTTGCCTTAAATAAAGAAGTGGATGCGAAAACAGCCGAACATATGAGC
AAAATTTTCTTAGAAATTATTATTGCGCCAAGTTTCTCTGAAGAAGCGTTTGCCATTTTAGCGAAAAAGAAAAATATTCG
CTTGTTAACTGTTCCGTTTGCCGGTTCCGTAAAAGGTTTCGAGAAAACATCTGTAAATGGTGGACTTCTTATTCAAGCGA
GCGATTCTGTTATAGAAGATACAGCGAGTTATGAAGTAGTAACGGAAAAACAACCTACAGAAGCGGAAATGAAAGCATTA
ATTGCTCAGTGGAAAATTGTGAAACATGTAAAATCCAATGCGATAGTAGTAGGATCTGATAAACAAACACTTGGAATTGG
TGCAGGACAAATGAATCGAATCGGTTCCGCGTTAATTGCACTTGAGCAAGCTGGCGAGAAAGCAAAAGGTGCTGTACTTG
CCTCGGATGCCTTTTTTCCAATGGATGATACGGTCGAAGCTGCGGCAAAAGCGGGAATCACAGCGATTATTCAGCCTGGT
GGATCCATTAAAGACAAGGAATCCATTGAGATGGCAAACAAATATGGTATTTCCATGGTTCTAACTCATGTACGACACTT
CAAACATTAA

Upstream 100 bases:

>100_bases
CTGTAGATACTTTAGCTGGAAAAATTCATCAAGTAGAACATATTTTTTATCCAAAAGTGATTCGAGGATTAATTCAAAAT
GGAGGGAACGATTAATTATC

Downstream 100 bases:

>100_bases
TCCTCAAAAAGGACGTGTAAATAAATGAACTTATTAGTAGTTGGTAGCGGTGGTAGAGAACATGCTATTAGTAAAAAATT
ATTAGAATCCAACAATGTCG

Product: bifunctional phosphoribosylaminoimidazolecarboxamide formyltransferase/IMP cyclohydrolase

Products: NA

Alternate protein names: Phosphoribosylaminoimidazolecarboxamide formyltransferase; AICAR transformylase; IMP cyclohydrolase; ATIC; IMP synthase; Inosinicase [H]

Number of amino acids: Translated: 509; Mature: 509

Protein sequence:

>509_residues
MKRALISVSDKNGIVPFAEKLVELGVEIISTGGTKAAFEQAGVPVTGIEDVTEFPEMLDGRVKTLHPAIHGGLLARRDTA
EHMEAIAAHDIKPIDLVVVNLYPFQETIQKSGVTLEEAIENIDIGGPSMLRSAAKNYAAVTVVVDTADYDTVLTELEEHG
ATTFETRQRLAAKVFRHTAAYDALIAEYLTNITGETFPEKVTLTYNRKQVLRYGENPHQDAAFYTEPGTVENSISSAKQL
HGKELSYNNIRDADAALKIASEFTEPVAVAVKHMNPCGVGVGENIEEAYLKAYEADETSIFGGIVALNKEVDAKTAEHMS
KIFLEIIIAPSFSEEAFAILAKKKNIRLLTVPFAGSVKGFEKTSVNGGLLIQASDSVIEDTASYEVVTEKQPTEAEMKAL
IAQWKIVKHVKSNAIVVGSDKQTLGIGAGQMNRIGSALIALEQAGEKAKGAVLASDAFFPMDDTVEAAAKAGITAIIQPG
GSIKDKESIEMANKYGISMVLTHVRHFKH

Sequences:

>Translated_509_residues
MKRALISVSDKNGIVPFAEKLVELGVEIISTGGTKAAFEQAGVPVTGIEDVTEFPEMLDGRVKTLHPAIHGGLLARRDTA
EHMEAIAAHDIKPIDLVVVNLYPFQETIQKSGVTLEEAIENIDIGGPSMLRSAAKNYAAVTVVVDTADYDTVLTELEEHG
ATTFETRQRLAAKVFRHTAAYDALIAEYLTNITGETFPEKVTLTYNRKQVLRYGENPHQDAAFYTEPGTVENSISSAKQL
HGKELSYNNIRDADAALKIASEFTEPVAVAVKHMNPCGVGVGENIEEAYLKAYEADETSIFGGIVALNKEVDAKTAEHMS
KIFLEIIIAPSFSEEAFAILAKKKNIRLLTVPFAGSVKGFEKTSVNGGLLIQASDSVIEDTASYEVVTEKQPTEAEMKAL
IAQWKIVKHVKSNAIVVGSDKQTLGIGAGQMNRIGSALIALEQAGEKAKGAVLASDAFFPMDDTVEAAAKAGITAIIQPG
GSIKDKESIEMANKYGISMVLTHVRHFKH
>Mature_509_residues
MKRALISVSDKNGIVPFAEKLVELGVEIISTGGTKAAFEQAGVPVTGIEDVTEFPEMLDGRVKTLHPAIHGGLLARRDTA
EHMEAIAAHDIKPIDLVVVNLYPFQETIQKSGVTLEEAIENIDIGGPSMLRSAAKNYAAVTVVVDTADYDTVLTELEEHG
ATTFETRQRLAAKVFRHTAAYDALIAEYLTNITGETFPEKVTLTYNRKQVLRYGENPHQDAAFYTEPGTVENSISSAKQL
HGKELSYNNIRDADAALKIASEFTEPVAVAVKHMNPCGVGVGENIEEAYLKAYEADETSIFGGIVALNKEVDAKTAEHMS
KIFLEIIIAPSFSEEAFAILAKKKNIRLLTVPFAGSVKGFEKTSVNGGLLIQASDSVIEDTASYEVVTEKQPTEAEMKAL
IAQWKIVKHVKSNAIVVGSDKQTLGIGAGQMNRIGSALIALEQAGEKAKGAVLASDAFFPMDDTVEAAAKAGITAIIQPG
GSIKDKESIEMANKYGISMVLTHVRHFKH

Specific function: De novo purine biosynthesis; ninth step. De novo purine biosynthesis; tenth step. [C]

COG id: COG0138

COG function: function code F; AICAR transformylase/IMP cyclohydrolase PurH (only IMP cyclohydrolase domain in Aful)

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the purH family [H]

Homologues:

Organism=Homo sapiens, GI20127454, Length=476, Percent_Identity=38.4453781512605, Blast_Score=271, Evalue=1e-72,
Organism=Escherichia coli, GI1790439, Length=525, Percent_Identity=51.2380952380952, Blast_Score=528, Evalue=1e-151,
Organism=Caenorhabditis elegans, GI71985564, Length=477, Percent_Identity=35.4297693920335, Blast_Score=260, Evalue=1e-69,
Organism=Caenorhabditis elegans, GI71985574, Length=308, Percent_Identity=25.974025974026, Blast_Score=81, Evalue=2e-15,
Organism=Saccharomyces cerevisiae, GI6323768, Length=475, Percent_Identity=36.4210526315789, Blast_Score=262, Evalue=1e-70,
Organism=Saccharomyces cerevisiae, GI6323056, Length=470, Percent_Identity=36.3829787234043, Blast_Score=256, Evalue=5e-69,
Organism=Drosophila melanogaster, GI24649832, Length=476, Percent_Identity=38.4453781512605, Blast_Score=272, Evalue=4e-73,

Paralogues:

None

Copy number: 160 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 640 Molecules/Cell In: Growth-Phase, Minimal-Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002695
- InterPro:   IPR013982
- InterPro:   IPR016193
- InterPro:   IPR011607 [H]

Pfam domain/function: PF01808 AICARFT_IMPCHas; PF02142 MGS [H]

EC number: =2.1.2.3; =3.5.4.10 [H]

Molecular weight: Translated: 54898; Mature: 54898

Theoretical pI: Translated: 5.11; Mature: 5.11

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.2 %Cys     (Translated Protein)
2.2 %Met     (Translated Protein)
2.4 %Cys+Met (Translated Protein)
0.2 %Cys     (Mature Protein)
2.2 %Met     (Mature Protein)
2.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKRALISVSDKNGIVPFAEKLVELGVEIISTGGTKAAFEQAGVPVTGIEDVTEFPEMLDG
CCCEEEEECCCCCCCHHHHHHHHHCHHHCCCCCCHHHHHHCCCCCCCHHHHHHHHHHHCC
RVKTLHPAIHGGLLARRDTAEHMEAIAAHDIKPIDLVVVNLYPFQETIQKSGVTLEEAIE
CHHHHHHHHHCCHHCCCCHHHHHHHHHHCCCCCEEEEEEEEECHHHHHHHCCCCHHHHHH
NIDIGGPSMLRSAAKNYAAVTVVVDTADYDTVLTELEEHGATTFETRQRLAAKVFRHTAA
HCCCCCHHHHHHHHCCCEEEEEEEECCCHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHH
YDALIAEYLTNITGETFPEKVTLTYNRKQVLRYGENPHQDAAFYTEPGTVENSISSAKQL
HHHHHHHHHHHCCCCCCCCEEEEEECHHHHHHCCCCCCCCCCEEECCCCHHHHHHHHHHH
HGKELSYNNIRDADAALKIASEFTEPVAVAVKHMNPCGVGVGENIEEAYLKAYEADETSI
CCCCCCCCCCCCHHHHHHHHHHHCHHHHHHHHCCCCCCCCCCCCHHHHHHHHHCCCCCHH
FGGIVALNKEVDAKTAEHMSKIFLEIIIAPSFSEEAFAILAKKKNIRLLTVPFAGSVKGF
HHEEEEECCCCCHHHHHHHHHHHHHHHCCCCCCHHHHEEEEECCCEEEEEECCCCCCCCC
EKTSVNGGLLIQASDSVIEDTASYEVVTEKQPTEAEMKALIAQWKIVKHVKSNAIVVGSD
CCCCCCCCEEEEECCHHHHCCCCEEEEECCCCCHHHHHHHHHHHHHHHHHCCCEEEECCC
KQTLGIGAGQMNRIGSALIALEQAGEKAKGAVLASDAFFPMDDTVEAAAKAGITAIIQPG
CCEEECCCCHHHHHHHHHHHHHHCCCCCCCEEEECCCCCCCCHHHHHHHHCCCEEEECCC
GSIKDKESIEMANKYGISMVLTHVRHFKH
CCCCCHHHHHHHHHHHHHHHHHHHHHHCC
>Mature Secondary Structure
MKRALISVSDKNGIVPFAEKLVELGVEIISTGGTKAAFEQAGVPVTGIEDVTEFPEMLDG
CCCEEEEECCCCCCCHHHHHHHHHCHHHCCCCCCHHHHHHCCCCCCCHHHHHHHHHHHCC
RVKTLHPAIHGGLLARRDTAEHMEAIAAHDIKPIDLVVVNLYPFQETIQKSGVTLEEAIE
CHHHHHHHHHCCHHCCCCHHHHHHHHHHCCCCCEEEEEEEEECHHHHHHHCCCCHHHHHH
NIDIGGPSMLRSAAKNYAAVTVVVDTADYDTVLTELEEHGATTFETRQRLAAKVFRHTAA
HCCCCCHHHHHHHHCCCEEEEEEEECCCHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHH
YDALIAEYLTNITGETFPEKVTLTYNRKQVLRYGENPHQDAAFYTEPGTVENSISSAKQL
HHHHHHHHHHHCCCCCCCCEEEEEECHHHHHHCCCCCCCCCCEEECCCCHHHHHHHHHHH
HGKELSYNNIRDADAALKIASEFTEPVAVAVKHMNPCGVGVGENIEEAYLKAYEADETSI
CCCCCCCCCCCCHHHHHHHHHHHCHHHHHHHHCCCCCCCCCCCCHHHHHHHHHCCCCCHH
FGGIVALNKEVDAKTAEHMSKIFLEIIIAPSFSEEAFAILAKKKNIRLLTVPFAGSVKGF
HHEEEEECCCCCHHHHHHHHHHHHHHHCCCCCCHHHHEEEEECCCEEEEEECCCCCCCCC
EKTSVNGGLLIQASDSVIEDTASYEVVTEKQPTEAEMKALIAQWKIVKHVKSNAIVVGSD
CCCCCCCCEEEEECCHHHHCCCCEEEEECCCCCHHHHHHHHHHHHHHHHHCCCEEEECCC
KQTLGIGAGQMNRIGSALIALEQAGEKAKGAVLASDAFFPMDDTVEAAAKAGITAIIQPG
CCEEECCCCHHHHHHHHHHHHHHCCCCCCCEEEECCCCCCCCHHHHHHHHCCCEEEECCC
GSIKDKESIEMANKYGISMVLTHVRHFKH
CCCCCHHHHHHHHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11679669 [H]