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The map label for this gene is purD

Identifier: 226224367

GI number: 226224367

Start: 1821665

End: 1822927

Strand: Reverse

Name: purD

Synonym: Lm4b_01778

Alternate gene names: 226224367

Gene position: 1822927-1821665 (Counterclockwise)

Preceding gene: 226224368

Following gene: 226224366

Centisome position: 62.59

GC content: 40.46

Gene sequence:

>1263_bases
ATGAACTTATTAGTAGTTGGTAGCGGTGGTAGAGAACATGCTATTAGTAAAAAATTATTAGAATCCAACAATGTCGAAAA
AGTATACTGTGCTCCGGGAAACGACGGAATGCGTTTAGACAACATCCAACTCGTAGCTATTTCTGAAACGGATAAAGCTG
GTTTAATTGATTTTGCTAAAAAAGCAGAAATTGCTTTTGTTATTGTTGGGCCTGAAGTGCCTCTTTTAGAAGGCGTTGTC
GATGCTCTCGAAGAAGCTGGAATTAAAGCATTCGGTCCAAAAGCAAATGCAGCATTAATTGAAGGCAGCAAAGATTTTGC
GAAACAATTTATGGAGAAATATGCTATTCCAACCGCAGCATCAAAAACCTTCACCGATTATGCGGAAGCGAAAGCATACT
TGGATGAGCGAGGAGTGCCAATCGTCATTAAAGCAGATGGACTCGCTGCTGGAAAAGGAGTCACTGTAGCGTTAGAAATG
GAAGAAGCAGTTCTTGCATTAAAAGATATGATGCTAGAAGAAAAATTCGGTGACGCATCTCTCAAGGTCGTTATCGAGGA
CTTTTTAGCGGGAGAAGAGTTTTCATTGATGGCGTTTGTAAATGGGGAAGAAGTTTATCCAATGGCTATCGCGCAAGATC
ATAAACGCGCTTATGAAGGCGATAAAGGACCAAATACTGGCGGAATGGGAGCTTATTCTCCGGTACCACATATTTCTGAA
ACAGTAGTAGACGAAGCTGTCGAAAAAATTCTTCTTCCAGCTGCAAAAGGAATGGTAAAAGAAGGTCGTTATTTCCGCGG
AATTCTCTATGCTGGGCTTATCTTAACCGCAGAAGGCCCAAAAGTAATTGAGTTTAACGCACGCTTTGGTGATCCGGAAA
CACAAGTTGTTTTGCCTCGTTTAGAGAGTGATTTTGCAGCACTGATAGACGCTTTACTCCATAACGAAAAACCAGATGTG
CGCTTTAAAAAATCAGGGATTACGCTAGGAGTTGTCCTAGCAAGCGCCGGTTATCCAGAGCATTATGAAAAAGGAAACAA
ATTAACTGGTCTAAATGATGTTGCAGAAGATGTAGCGATTTATCATGCAGGTACAAAACAAGATGAAAATGACGATTTTA
TTTCTGATGGTGGACGCGTCTTGCTACTAGCAAAAGAAGCAGAAACGATGACTGATGCACGCACATTACTTTATCCAGAA
ATGCAAAAACTAGATAACCCGAATTTCTTTTATCGAATTGATATTGGCACAAAAGCAGAATAA

Upstream 100 bases:

>100_bases
CAAGGAATCCATTGAGATGGCAAACAAATATGGTATTTCCATGGTTCTAACTCATGTACGACACTTCAAACATTAATCCT
CAAAAAGGACGTGTAAATAA

Downstream 100 bases:

>100_bases
TTGAAAAGTACTATCTCAAGGATAAATTCCTAGATAGTACTTTTTTTGTTCATTAAGTGAAGAGAAACGTTTGTGTTTTA
CGCGTTGGTACGTTAAAATA

Product: phosphoribosylamine--glycine ligase

Products: NA

Alternate protein names: GARS; Glycinamide ribonucleotide synthetase; Phosphoribosylglycinamide synthetase [H]

Number of amino acids: Translated: 420; Mature: 420

Protein sequence:

>420_residues
MNLLVVGSGGREHAISKKLLESNNVEKVYCAPGNDGMRLDNIQLVAISETDKAGLIDFAKKAEIAFVIVGPEVPLLEGVV
DALEEAGIKAFGPKANAALIEGSKDFAKQFMEKYAIPTAASKTFTDYAEAKAYLDERGVPIVIKADGLAAGKGVTVALEM
EEAVLALKDMMLEEKFGDASLKVVIEDFLAGEEFSLMAFVNGEEVYPMAIAQDHKRAYEGDKGPNTGGMGAYSPVPHISE
TVVDEAVEKILLPAAKGMVKEGRYFRGILYAGLILTAEGPKVIEFNARFGDPETQVVLPRLESDFAALIDALLHNEKPDV
RFKKSGITLGVVLASAGYPEHYEKGNKLTGLNDVAEDVAIYHAGTKQDENDDFISDGGRVLLLAKEAETMTDARTLLYPE
MQKLDNPNFFYRIDIGTKAE

Sequences:

>Translated_420_residues
MNLLVVGSGGREHAISKKLLESNNVEKVYCAPGNDGMRLDNIQLVAISETDKAGLIDFAKKAEIAFVIVGPEVPLLEGVV
DALEEAGIKAFGPKANAALIEGSKDFAKQFMEKYAIPTAASKTFTDYAEAKAYLDERGVPIVIKADGLAAGKGVTVALEM
EEAVLALKDMMLEEKFGDASLKVVIEDFLAGEEFSLMAFVNGEEVYPMAIAQDHKRAYEGDKGPNTGGMGAYSPVPHISE
TVVDEAVEKILLPAAKGMVKEGRYFRGILYAGLILTAEGPKVIEFNARFGDPETQVVLPRLESDFAALIDALLHNEKPDV
RFKKSGITLGVVLASAGYPEHYEKGNKLTGLNDVAEDVAIYHAGTKQDENDDFISDGGRVLLLAKEAETMTDARTLLYPE
MQKLDNPNFFYRIDIGTKAE
>Mature_420_residues
MNLLVVGSGGREHAISKKLLESNNVEKVYCAPGNDGMRLDNIQLVAISETDKAGLIDFAKKAEIAFVIVGPEVPLLEGVV
DALEEAGIKAFGPKANAALIEGSKDFAKQFMEKYAIPTAASKTFTDYAEAKAYLDERGVPIVIKADGLAAGKGVTVALEM
EEAVLALKDMMLEEKFGDASLKVVIEDFLAGEEFSLMAFVNGEEVYPMAIAQDHKRAYEGDKGPNTGGMGAYSPVPHISE
TVVDEAVEKILLPAAKGMVKEGRYFRGILYAGLILTAEGPKVIEFNARFGDPETQVVLPRLESDFAALIDALLHNEKPDV
RFKKSGITLGVVLASAGYPEHYEKGNKLTGLNDVAEDVAIYHAGTKQDENDDFISDGGRVLLLAKEAETMTDARTLLYPE
MQKLDNPNFFYRIDIGTKAE

Specific function: De novo purine biosynthesis; second step. [C]

COG id: COG0151

COG function: function code F; Phosphoribosylamine-glycine ligase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 ATP-grasp domain [H]

Homologues:

Organism=Homo sapiens, GI4503915, Length=425, Percent_Identity=44.7058823529412, Blast_Score=343, Evalue=2e-94,
Organism=Homo sapiens, GI209869995, Length=425, Percent_Identity=44.7058823529412, Blast_Score=343, Evalue=2e-94,
Organism=Homo sapiens, GI209869993, Length=425, Percent_Identity=44.7058823529412, Blast_Score=343, Evalue=2e-94,
Organism=Homo sapiens, GI28416899, Length=425, Percent_Identity=44.7058823529412, Blast_Score=343, Evalue=2e-94,
Organism=Escherichia coli, GI1790438, Length=427, Percent_Identity=48.2435597189695, Blast_Score=379, Evalue=1e-106,
Organism=Caenorhabditis elegans, GI17567511, Length=424, Percent_Identity=37.9716981132075, Blast_Score=256, Evalue=2e-68,
Organism=Saccharomyces cerevisiae, GI6321203, Length=440, Percent_Identity=39.7727272727273, Blast_Score=298, Evalue=9e-82,
Organism=Drosophila melanogaster, GI62471619, Length=389, Percent_Identity=43.7017994858612, Blast_Score=304, Evalue=9e-83,
Organism=Drosophila melanogaster, GI24582400, Length=389, Percent_Identity=43.7017994858612, Blast_Score=303, Evalue=1e-82,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR011761
- InterPro:   IPR013815
- InterPro:   IPR013816
- InterPro:   IPR013817
- InterPro:   IPR016185
- InterPro:   IPR020561
- InterPro:   IPR000115
- InterPro:   IPR020560
- InterPro:   IPR020559
- InterPro:   IPR020562
- InterPro:   IPR011054 [H]

Pfam domain/function: PF01071 GARS_A; PF02843 GARS_C; PF02844 GARS_N [H]

EC number: =6.3.4.13 [H]

Molecular weight: Translated: 45444; Mature: 45444

Theoretical pI: Translated: 4.48; Mature: 4.48

Prosite motif: PS50975 ATP_GRASP ; PS00184 GARS

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.2 %Cys     (Translated Protein)
2.9 %Met     (Translated Protein)
3.1 %Cys+Met (Translated Protein)
0.2 %Cys     (Mature Protein)
2.9 %Met     (Mature Protein)
3.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNLLVVGSGGREHAISKKLLESNNVEKVYCAPGNDGMRLDNIQLVAISETDKAGLIDFAK
CEEEEECCCCCHHHHHHHHHHCCCCCEEEECCCCCCCEECCEEEEEEECCCCCCHHHHHC
KAEIAFVIVGPEVPLLEGVVDALEEAGIKAFGPKANAALIEGSKDFAKQFMEKYAIPTAA
CCCEEEEEECCCCHHHHHHHHHHHHCCCEEECCCCCEEEEECCHHHHHHHHHHHCCCCCC
SKTFTDYAEAKAYLDERGVPIVIKADGLAAGKGVTVALEMEEAVLALKDMMLEEKFGDAS
CCHHHHHHHHHHHHHCCCCEEEEEECCCCCCCCEEEEEEHHHHHHHHHHHHHHHHCCCCH
LKVVIEDFLAGEEFSLMAFVNGEEVYPMAIAQDHKRAYEGDKGPNTGGMGAYSPVPHISE
HHHHHHHHHCCCCEEEEEEECCCEEEEEHHHHHHHHHHCCCCCCCCCCCCCCCCCCCHHH
TVVDEAVEKILLPAAKGMVKEGRYFRGILYAGLILTAEGPKVIEFNARFGDPETQVVLPR
HHHHHHHHHHHHHHHHHHHHCCHHHHHEEHEEEEEECCCCEEEEEECCCCCCCCEEEECC
LESDFAALIDALLHNEKPDVRFKKSGITLGVVLASAGYPEHYEKGNKLTGLNDVAEDVAI
CCCHHHHHHHHHHCCCCCCCEEECCCCEEEEEEECCCCCHHHHCCCCCCCHHHHHHHHEE
YHAGTKQDENDDFISDGGRVLLLAKEAETMTDARTLLYPEMQKLDNPNFFYRIDIGTKAE
EECCCCCCCCCCHHCCCCEEEEEECCCCHHHHHHHHCCHHHHHCCCCCEEEEEECCCCCC
>Mature Secondary Structure
MNLLVVGSGGREHAISKKLLESNNVEKVYCAPGNDGMRLDNIQLVAISETDKAGLIDFAK
CEEEEECCCCCHHHHHHHHHHCCCCCEEEECCCCCCCEECCEEEEEEECCCCCCHHHHHC
KAEIAFVIVGPEVPLLEGVVDALEEAGIKAFGPKANAALIEGSKDFAKQFMEKYAIPTAA
CCCEEEEEECCCCHHHHHHHHHHHHCCCEEECCCCCEEEEECCHHHHHHHHHHHCCCCCC
SKTFTDYAEAKAYLDERGVPIVIKADGLAAGKGVTVALEMEEAVLALKDMMLEEKFGDAS
CCHHHHHHHHHHHHHCCCCEEEEEECCCCCCCCEEEEEEHHHHHHHHHHHHHHHHCCCCH
LKVVIEDFLAGEEFSLMAFVNGEEVYPMAIAQDHKRAYEGDKGPNTGGMGAYSPVPHISE
HHHHHHHHHCCCCEEEEEEECCCEEEEEHHHHHHHHHHCCCCCCCCCCCCCCCCCCCHHH
TVVDEAVEKILLPAAKGMVKEGRYFRGILYAGLILTAEGPKVIEFNARFGDPETQVVLPR
HHHHHHHHHHHHHHHHHHHHCCHHHHHEEHEEEEEECCCCEEEEEECCCCCCCCEEEECC
LESDFAALIDALLHNEKPDVRFKKSGITLGVVLASAGYPEHYEKGNKLTGLNDVAEDVAI
CCCHHHHHHHHHHCCCCCCCEEECCCCEEEEEEECCCCCHHHHCCCCCCCHHHHHHHHEE
YHAGTKQDENDDFISDGGRVLLLAKEAETMTDARTLLYPEMQKLDNPNFFYRIDIGTKAE
EECCCCCCCCCCHHCCCCEEEEEECCCCHHHHHHHHCCHHHHHCCCCCEEEEEECCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11679669 [H]