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The map label for this gene is gatA

Identifier: 226224358

GI number: 226224358

Start: 1811371

End: 1812822

Strand: Reverse

Name: gatA

Synonym: Lm4b_01769

Alternate gene names: 226224358

Gene position: 1812822-1811371 (Counterclockwise)

Preceding gene: 226224359

Following gene: 226224357

Centisome position: 62.24

GC content: 37.6

Gene sequence:

>1452_bases
TTGGGTTTATTTGATTTTTCAGTAAAAGAACTACATGATAAATTAGTTAAAAAAGAGATTTCACCATTCGATTTAGTATC
AGAATCTTTCAACCGAATTGAATCTGTAGAGGACAAAGTTGGTTCTTTTATTACTTTAAATAAAGAGGCAGCATTTGGTG
TAGCAGAAGAACTTGGAGATGCGGGCATAGATCCAAACAACATGCTTTCTGGTCTTCCAATTGGGATTAAAGATAATATC
GTGACAAAAAACTTACGTACAACAGCAGCAAGTAAAATTTTAGAAAACTTCGATCCAATTTATGATGCGACCGTCGTTTC
TAAATTAAAAAATGCGCAAACAATTAATATCGGAAAATTAAACATGGATGAATTTGCGATGGGATCTTCGACAGAAACAT
CGTATTTCCACAAAACACATAACCCATGGGATTTATCAAGAGTTCCTGGTGGTTCTTCAGGTGGTAGTGCATCCGCAGTT
GCAGCAGGCGAAGTTTTATTCTCGCTTGGTAGTGACACTGGTGGATCGATTCGCCAACCAGCAGCTTTTTGTGGGGTAGT
CGGAATGAAACCTACATATGGCCGTGTATCTCGTTTTGGTTTAATTGCCTTTGCGTCTTCTTTAGACCAAATCGGCCCAA
TTACAAAAAATGTAGAAGACAACGCTTATTTATTAGAAGCTATTTCTGGTTTAGATGCGAATGATTCTACATCGATTAAC
CAACCAGTTGAACGCTTCTCAAATAGCTTAACAGGAGACATTAAAGGCTTACGCATCGGTGTTCCAAAAGAATATCTTGG
CGAAGGCGTTGATCCTGGCGTAAAACAAGCTGTATTAGATGCGCTTAAAACACTCGAAAAACTGGGCGCTACTTGGGATG
AAGTTTCTTTACCTCATTCTGAATACGGTGTAGCAAGTTATTACATTTTAGCATCTAGTGAAGCTTCTTCTAATCTATCT
CGTTTTGACGGCGTTCGTTACGGATACCGTTCTCCAAACGCAACTACTTTAGAAGAACTTTATACAAAAACTCGTTCTGA
AGGCTTTGGCGATGAAGTAAAACGTCGTATTATGCTTGGAACATATGCGTTAAGCTCTGGTTATTACGATGCTTACTACA
AAAAAGCACAACAAGCGCGTACGTTAATTAAGCAAGACTTTGTTAATGTATTTGAAAACTATGATGTTATTATCGGACCT
AGTTCCCCGACAACAGCATTTAAAATTGACGGAATGATTAATGATCCAATTACAATGTATTCCAATGATATTTTGACTGT
TCCAATTAACTTAGCAGGCGTACCAGCTATTTCTGTTCCTTGTGGATTCTCGGATGGTTTACCAGTTGGCTTGCAAATTA
TCGGTAACTACTTTGAAGAATCCTTACTTTACAAAGTAGCGCATGCTTTTGAACAAGAAACAACATTCCATAAAGAAAAA
CCAAACTTATAG

Upstream 100 bases:

>100_bases
ATCGCAAAGAAGTACTAAAAAATGCGCCTGATGAACAAGATGGCATGTTCAAAGTACCGACAATTATGGAACAATAAATA
GGCTAAGGAGGGAAAATATT

Downstream 100 bases:

>100_bases
GAGGCGTAGCAATGAATTTTGAAACAGTTATTGGACTTGAGGTTCACGTAGAGTTAAAAACCAATTCAAAAATATTTTCT
TCTGCGCCAGCTCATTTTGG

Product: aspartyl/glutamyl-tRNA amidotransferase subunit A

Products: NA

Alternate protein names: Glu-ADT subunit A [H]

Number of amino acids: Translated: 483; Mature: 482

Protein sequence:

>483_residues
MGLFDFSVKELHDKLVKKEISPFDLVSESFNRIESVEDKVGSFITLNKEAAFGVAEELGDAGIDPNNMLSGLPIGIKDNI
VTKNLRTTAASKILENFDPIYDATVVSKLKNAQTINIGKLNMDEFAMGSSTETSYFHKTHNPWDLSRVPGGSSGGSASAV
AAGEVLFSLGSDTGGSIRQPAAFCGVVGMKPTYGRVSRFGLIAFASSLDQIGPITKNVEDNAYLLEAISGLDANDSTSIN
QPVERFSNSLTGDIKGLRIGVPKEYLGEGVDPGVKQAVLDALKTLEKLGATWDEVSLPHSEYGVASYYILASSEASSNLS
RFDGVRYGYRSPNATTLEELYTKTRSEGFGDEVKRRIMLGTYALSSGYYDAYYKKAQQARTLIKQDFVNVFENYDVIIGP
SSPTTAFKIDGMINDPITMYSNDILTVPINLAGVPAISVPCGFSDGLPVGLQIIGNYFEESLLYKVAHAFEQETTFHKEK
PNL

Sequences:

>Translated_483_residues
MGLFDFSVKELHDKLVKKEISPFDLVSESFNRIESVEDKVGSFITLNKEAAFGVAEELGDAGIDPNNMLSGLPIGIKDNI
VTKNLRTTAASKILENFDPIYDATVVSKLKNAQTINIGKLNMDEFAMGSSTETSYFHKTHNPWDLSRVPGGSSGGSASAV
AAGEVLFSLGSDTGGSIRQPAAFCGVVGMKPTYGRVSRFGLIAFASSLDQIGPITKNVEDNAYLLEAISGLDANDSTSIN
QPVERFSNSLTGDIKGLRIGVPKEYLGEGVDPGVKQAVLDALKTLEKLGATWDEVSLPHSEYGVASYYILASSEASSNLS
RFDGVRYGYRSPNATTLEELYTKTRSEGFGDEVKRRIMLGTYALSSGYYDAYYKKAQQARTLIKQDFVNVFENYDVIIGP
SSPTTAFKIDGMINDPITMYSNDILTVPINLAGVPAISVPCGFSDGLPVGLQIIGNYFEESLLYKVAHAFEQETTFHKEK
PNL
>Mature_482_residues
GLFDFSVKELHDKLVKKEISPFDLVSESFNRIESVEDKVGSFITLNKEAAFGVAEELGDAGIDPNNMLSGLPIGIKDNIV
TKNLRTTAASKILENFDPIYDATVVSKLKNAQTINIGKLNMDEFAMGSSTETSYFHKTHNPWDLSRVPGGSSGGSASAVA
AGEVLFSLGSDTGGSIRQPAAFCGVVGMKPTYGRVSRFGLIAFASSLDQIGPITKNVEDNAYLLEAISGLDANDSTSINQ
PVERFSNSLTGDIKGLRIGVPKEYLGEGVDPGVKQAVLDALKTLEKLGATWDEVSLPHSEYGVASYYILASSEASSNLSR
FDGVRYGYRSPNATTLEELYTKTRSEGFGDEVKRRIMLGTYALSSGYYDAYYKKAQQARTLIKQDFVNVFENYDVIIGPS
SPTTAFKIDGMINDPITMYSNDILTVPINLAGVPAISVPCGFSDGLPVGLQIIGNYFEESLLYKVAHAFEQETTFHKEKP
NL

Specific function: Furnishes a means for formation of correctly charged Gln-tRNA(Gln) through the transamidation of misacylated Glu- tRNA(Gln) in organisms which lack glutaminyl-tRNA synthetase. The reaction takes place in the presence of glutamine and ATP through an activa

COG id: COG0154

COG function: function code J; Asp-tRNAAsn/Glu-tRNAGln amidotransferase A subunit and related amidases

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the amidase family [H]

Homologues:

Organism=Homo sapiens, GI222831590, Length=505, Percent_Identity=37.6237623762376, Blast_Score=317, Evalue=2e-86,
Organism=Homo sapiens, GI195972892, Length=504, Percent_Identity=24.8015873015873, Blast_Score=124, Evalue=3e-28,
Organism=Homo sapiens, GI166795287, Length=288, Percent_Identity=27.4305555555556, Blast_Score=69, Evalue=7e-12,
Organism=Caenorhabditis elegans, GI17543272, Length=459, Percent_Identity=36.8191721132898, Blast_Score=271, Evalue=7e-73,
Organism=Caenorhabditis elegans, GI17537465, Length=479, Percent_Identity=25.0521920668058, Blast_Score=118, Evalue=8e-27,
Organism=Caenorhabditis elegans, GI17556264, Length=288, Percent_Identity=30.9027777777778, Blast_Score=110, Evalue=2e-24,
Organism=Caenorhabditis elegans, GI71990152, Length=310, Percent_Identity=30, Blast_Score=107, Evalue=2e-23,
Organism=Caenorhabditis elegans, GI17538252, Length=303, Percent_Identity=27.3927392739274, Blast_Score=97, Evalue=2e-20,
Organism=Caenorhabditis elegans, GI17556276, Length=257, Percent_Identity=31.5175097276265, Blast_Score=77, Evalue=1e-14,
Organism=Caenorhabditis elegans, GI17556278, Length=257, Percent_Identity=31.5175097276265, Blast_Score=77, Evalue=1e-14,
Organism=Caenorhabditis elegans, GI17538254, Length=298, Percent_Identity=28.1879194630872, Blast_Score=76, Evalue=4e-14,
Organism=Saccharomyces cerevisiae, GI6323950, Length=461, Percent_Identity=36.6594360086768, Blast_Score=250, Evalue=3e-67,
Organism=Saccharomyces cerevisiae, GI6319685, Length=416, Percent_Identity=30.7692307692308, Blast_Score=119, Evalue=1e-27,
Organism=Saccharomyces cerevisiae, GI6320448, Length=297, Percent_Identity=26.9360269360269, Blast_Score=66, Evalue=1e-11,
Organism=Drosophila melanogaster, GI24648113, Length=493, Percent_Identity=39.7565922920893, Blast_Score=310, Evalue=1e-84,
Organism=Drosophila melanogaster, GI24644968, Length=512, Percent_Identity=28.3203125, Blast_Score=135, Evalue=6e-32,
Organism=Drosophila melanogaster, GI24652985, Length=493, Percent_Identity=26.7748478701826, Blast_Score=130, Evalue=3e-30,
Organism=Drosophila melanogaster, GI19922090, Length=493, Percent_Identity=26.7748478701826, Blast_Score=130, Evalue=3e-30,
Organism=Drosophila melanogaster, GI24652981, Length=493, Percent_Identity=26.7748478701826, Blast_Score=130, Evalue=3e-30,
Organism=Drosophila melanogaster, GI24652983, Length=493, Percent_Identity=26.7748478701826, Blast_Score=130, Evalue=3e-30,
Organism=Drosophila melanogaster, GI161078093, Length=517, Percent_Identity=28.4332688588008, Blast_Score=126, Evalue=4e-29,
Organism=Drosophila melanogaster, GI21356731, Length=487, Percent_Identity=24.0246406570842, Blast_Score=94, Evalue=3e-19,
Organism=Drosophila melanogaster, GI24648435, Length=241, Percent_Identity=29.045643153527, Blast_Score=86, Evalue=5e-17,
Organism=Drosophila melanogaster, GI24648437, Length=241, Percent_Identity=29.045643153527, Blast_Score=86, Evalue=5e-17,
Organism=Drosophila melanogaster, GI45550774, Length=231, Percent_Identity=30.3030303030303, Blast_Score=86, Evalue=5e-17,
Organism=Drosophila melanogaster, GI24648441, Length=166, Percent_Identity=36.144578313253, Blast_Score=85, Evalue=1e-16,
Organism=Drosophila melanogaster, GI24648439, Length=166, Percent_Identity=36.144578313253, Blast_Score=85, Evalue=1e-16,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000120
- InterPro:   IPR020556
- InterPro:   IPR004412 [H]

Pfam domain/function: PF01425 Amidase [H]

EC number: 6.3.5.-

Molecular weight: Translated: 52339; Mature: 52208

Theoretical pI: Translated: 4.71; Mature: 4.71

Prosite motif: PS00571 AMIDASES

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
1.7 %Met     (Translated Protein)
2.1 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
1.5 %Met     (Mature Protein)
1.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MGLFDFSVKELHDKLVKKEISPFDLVSESFNRIESVEDKVGSFITLNKEAAFGVAEELGD
CCCCCHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHCCCEEEECCHHHHHHHHHHCC
AGIDPNNMLSGLPIGIKDNIVTKNLRTTAASKILENFDPIYDATVVSKLKNAQTINIGKL
CCCCHHHHHCCCCCCCCCCCCHHHHHHHHHHHHHHHCCCHHHHHHHHHHCCCCEEEECCC
NMDEFAMGSSTETSYFHKTHNPWDLSRVPGGSSGGSASAVAAGEVLFSLGSDTGGSIRQP
CHHHHCCCCCCCCHHHCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHCCCCCCCCCCCC
AAFCGVVGMKPTYGRVSRFGLIAFASSLDQIGPITKNVEDNAYLLEAISGLDANDSTSIN
HHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHCCCCCCCCCHH
QPVERFSNSLTGDIKGLRIGVPKEYLGEGVDPGVKQAVLDALKTLEKLGATWDEVSLPHS
HHHHHHHCCCCCCCCCEEECCCHHHHCCCCCHHHHHHHHHHHHHHHHHCCCHHHCCCCCC
EYGVASYYILASSEASSNLSRFDGVRYGYRSPNATTLEELYTKTRSEGFGDEVKRRIMLG
CCCCEEEEEEECCCCCCCHHHHCCCCCCCCCCCCHHHHHHHHHHHCCCCCHHHHHHHHHH
TYALSSGYYDAYYKKAQQARTLIKQDFVNVFENYDVIIGPSSPTTAFKIDGMINDPITMY
HHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCEEECCCCCCCEEEECCCCCCCHHEE
SNDILTVPINLAGVPAISVPCGFSDGLPVGLQIIGNYFEESLLYKVAHAFEQETTFHKEK
CCCEEEEEEEECCCCEEECCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCC
PNL
CCC
>Mature Secondary Structure 
GLFDFSVKELHDKLVKKEISPFDLVSESFNRIESVEDKVGSFITLNKEAAFGVAEELGD
CCCCHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHCCCEEEECCHHHHHHHHHHCC
AGIDPNNMLSGLPIGIKDNIVTKNLRTTAASKILENFDPIYDATVVSKLKNAQTINIGKL
CCCCHHHHHCCCCCCCCCCCCHHHHHHHHHHHHHHHCCCHHHHHHHHHHCCCCEEEECCC
NMDEFAMGSSTETSYFHKTHNPWDLSRVPGGSSGGSASAVAAGEVLFSLGSDTGGSIRQP
CHHHHCCCCCCCCHHHCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHCCCCCCCCCCCC
AAFCGVVGMKPTYGRVSRFGLIAFASSLDQIGPITKNVEDNAYLLEAISGLDANDSTSIN
HHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHCCCCCCCCCHH
QPVERFSNSLTGDIKGLRIGVPKEYLGEGVDPGVKQAVLDALKTLEKLGATWDEVSLPHS
HHHHHHHCCCCCCCCCEEECCCHHHHCCCCCHHHHHHHHHHHHHHHHHCCCHHHCCCCCC
EYGVASYYILASSEASSNLSRFDGVRYGYRSPNATTLEELYTKTRSEGFGDEVKRRIMLG
CCCCEEEEEEECCCCCCCHHHHCCCCCCCCCCCCHHHHHHHHHHHCCCCCHHHHHHHHHH
TYALSSGYYDAYYKKAQQARTLIKQDFVNVFENYDVIIGPSSPTTAFKIDGMINDPITMY
HHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCEEECCCCCCCEEEECCCCCCCHHEE
SNDILTVPINLAGVPAISVPCGFSDGLPVGLQIIGNYFEESLLYKVAHAFEQETTFHKEK
CCCEEEEEEEECCCCEEECCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCC
PNL
CCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11679669 [H]