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The map label for this gene is gatB

Identifier: 226224357

GI number: 226224357

Start: 1809929

End: 1811359

Strand: Reverse

Name: gatB

Synonym: Lm4b_01768

Alternate gene names: 226224357

Gene position: 1811359-1809929 (Counterclockwise)

Preceding gene: 226224358

Following gene: 226224356

Centisome position: 62.19

GC content: 38.09

Gene sequence:

>1431_bases
ATGAATTTTGAAACAGTTATTGGACTTGAGGTTCACGTAGAGTTAAAAACCAATTCAAAAATATTTTCTTCTGCGCCAGC
TCATTTTGGAGCAGAACCAAATACAAATACAACCGTGGTAGACTTAGGTATGCCAGGTGTTTTACCAGTTTTAAATAAAC
GTGCAGTGGAATTTGGTATGAAAGCGGCTATGGCGATTAACTGTGAAATTGCTAAACATACAAAATTCGACCGTAAAAAC
TATTTTTATCCAGATAATCCGAAAGCATACCAAATTTCTCAATTCGATAAACCAATTGGCGAACATGGCTGGATTGAAAT
CGAAGTTGGCGGTAAAAAGAAAAAAATTGGCATCACTCGTCTTCATTTAGAAGAAGATGCTGGGAAAAATACCCATACTT
CTCATGGTTATTCGTTAGTGGATATTAACCGTCAAGGAACACCACTTATCGAAATCGTTTCAGAACCAGACATTCGTTCT
GCAGAAGAAGCTTATGCCTACTTAGAAAAATTAAAATCTATCATTCAATACACTGGCGTATCCGATGTGAAAATGGAAGA
AGGTTCAATGCGCTGTGACGCCAATATTTCTATCCGACCAATCGGCCAAGAAGAATTTGGCGTAAAAACAGAACTTAAAA
ACCTAAACTCCTTCAACAACGTACGTAAAGGTATCGAATATGAAGAAAAACGCCAAGCTGAAGTGCTTAAGTCTGGCGGG
ATTATCGAACAAGAAACTCGTCGTTTTGAAGAAGCAACTGGAAAAACCTCTTTGATGCGAATTAAAGAAGGATCCGACGA
TTATCGTTATTTCCCAGAACCAGATTTAGTAGATTTATTTATTGATGACGCTTGGAAAGAACGTATTCGTGCAGAAATTC
CTGAACTTCCAGACAAACGTCAAATTCGTTACATTAACGATCTTGGTTTGCCAGCATATGATGCAATGGTTCTCACGCTT
ACAAAAGAAATGTCTGACTTTTTTGAAGCAACTCTTGCAGCTGGAGCAGATGCGAAACAAGCATCAAACTGGCTAATGGG
CGAAGTTTCTGCCTACTTAAACGCAGAACAAAAAGAACTTCATGAAACAGGACTAACTCCTGAAAACCTTGCTGGCATGA
TTAAATTAATCGAAGCTGGTACTATTTCTTCTAAAATCGCTAAAAAAGTTTTCCGCGAATTAGCGCAAAATGGTGGCGAT
GCAGAGCAAGTAGTAAAAGACAAAGGACTTGTCCAAATTTCCGATGAAGGAGCTTTACGCACTATCATTAGCGAAATTCT
AGATAACAATGAGCAATCTATTGTCGACTTCAAAAATGGTAAGGATCGTGCGGTTGGATTCTTAGTTGGCCAAGTAATGA
AAGCAACCAAAGGGCAAGCAAACCCACCAATGGTTAATAAATTATTACTAGAAGAAATGAACAAACGTTAA

Upstream 100 bases:

>100_bases
GTAACTACTTTGAAGAATCCTTACTTTACAAAGTAGCGCATGCTTTTGAACAAGAAACAACATTCCATAAAGAAAAACCA
AACTTATAGGAGGCGTAGCA

Downstream 100 bases:

>100_bases
ACAATGTAGCAGTGAGGTATTTTGCCTCGCTGCTTTTATATTTATTAGGAATTTATAGTTAAATTTGCTATAATATTACT
ATCATGTGTCAAAACGCGCT

Product: aspartyl/glutamyl-tRNA amidotransferase subunit B

Products: NA

Alternate protein names: Asp/Glu-ADT subunit B [H]

Number of amino acids: Translated: 476; Mature: 476

Protein sequence:

>476_residues
MNFETVIGLEVHVELKTNSKIFSSAPAHFGAEPNTNTTVVDLGMPGVLPVLNKRAVEFGMKAAMAINCEIAKHTKFDRKN
YFYPDNPKAYQISQFDKPIGEHGWIEIEVGGKKKKIGITRLHLEEDAGKNTHTSHGYSLVDINRQGTPLIEIVSEPDIRS
AEEAYAYLEKLKSIIQYTGVSDVKMEEGSMRCDANISIRPIGQEEFGVKTELKNLNSFNNVRKGIEYEEKRQAEVLKSGG
IIEQETRRFEEATGKTSLMRIKEGSDDYRYFPEPDLVDLFIDDAWKERIRAEIPELPDKRQIRYINDLGLPAYDAMVLTL
TKEMSDFFEATLAAGADAKQASNWLMGEVSAYLNAEQKELHETGLTPENLAGMIKLIEAGTISSKIAKKVFRELAQNGGD
AEQVVKDKGLVQISDEGALRTIISEILDNNEQSIVDFKNGKDRAVGFLVGQVMKATKGQANPPMVNKLLLEEMNKR

Sequences:

>Translated_476_residues
MNFETVIGLEVHVELKTNSKIFSSAPAHFGAEPNTNTTVVDLGMPGVLPVLNKRAVEFGMKAAMAINCEIAKHTKFDRKN
YFYPDNPKAYQISQFDKPIGEHGWIEIEVGGKKKKIGITRLHLEEDAGKNTHTSHGYSLVDINRQGTPLIEIVSEPDIRS
AEEAYAYLEKLKSIIQYTGVSDVKMEEGSMRCDANISIRPIGQEEFGVKTELKNLNSFNNVRKGIEYEEKRQAEVLKSGG
IIEQETRRFEEATGKTSLMRIKEGSDDYRYFPEPDLVDLFIDDAWKERIRAEIPELPDKRQIRYINDLGLPAYDAMVLTL
TKEMSDFFEATLAAGADAKQASNWLMGEVSAYLNAEQKELHETGLTPENLAGMIKLIEAGTISSKIAKKVFRELAQNGGD
AEQVVKDKGLVQISDEGALRTIISEILDNNEQSIVDFKNGKDRAVGFLVGQVMKATKGQANPPMVNKLLLEEMNKR
>Mature_476_residues
MNFETVIGLEVHVELKTNSKIFSSAPAHFGAEPNTNTTVVDLGMPGVLPVLNKRAVEFGMKAAMAINCEIAKHTKFDRKN
YFYPDNPKAYQISQFDKPIGEHGWIEIEVGGKKKKIGITRLHLEEDAGKNTHTSHGYSLVDINRQGTPLIEIVSEPDIRS
AEEAYAYLEKLKSIIQYTGVSDVKMEEGSMRCDANISIRPIGQEEFGVKTELKNLNSFNNVRKGIEYEEKRQAEVLKSGG
IIEQETRRFEEATGKTSLMRIKEGSDDYRYFPEPDLVDLFIDDAWKERIRAEIPELPDKRQIRYINDLGLPAYDAMVLTL
TKEMSDFFEATLAAGADAKQASNWLMGEVSAYLNAEQKELHETGLTPENLAGMIKLIEAGTISSKIAKKVFRELAQNGGD
AEQVVKDKGLVQISDEGALRTIISEILDNNEQSIVDFKNGKDRAVGFLVGQVMKATKGQANPPMVNKLLLEEMNKR

Specific function: Allows the formation of correctly charged Asn-tRNA(Asn) or Gln-tRNA(Gln) through the transamidation of misacylated Asp- tRNA(Asn) or Glu-tRNA(Gln) in organisms which lack either or both of asparaginyl-tRNA or glutaminyl-tRNA synthetases. The reaction take

COG id: COG0064

COG function: function code J; Asp-tRNAAsn/Glu-tRNAGln amidotransferase B subunit (PET112 homolog)

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the gatB/gatE family. GatB subfamily [H]

Homologues:

Organism=Homo sapiens, GI4758894, Length=490, Percent_Identity=37.5510204081633, Blast_Score=318, Evalue=8e-87,
Organism=Caenorhabditis elegans, GI115534094, Length=303, Percent_Identity=38.9438943894389, Blast_Score=214, Evalue=8e-56,
Organism=Saccharomyces cerevisiae, GI6319391, Length=502, Percent_Identity=30.2788844621514, Blast_Score=212, Evalue=1e-55,
Organism=Drosophila melanogaster, GI45550808, Length=482, Percent_Identity=32.3651452282158, Blast_Score=262, Evalue=4e-70,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR017959
- InterPro:   IPR018027
- InterPro:   IPR003789
- InterPro:   IPR004413
- InterPro:   IPR006075
- InterPro:   IPR017958 [H]

Pfam domain/function: PF02934 GatB_N; PF02637 GatB_Yqey [H]

EC number: 6.3.5.-

Molecular weight: Translated: 53208; Mature: 53208

Theoretical pI: Translated: 5.16; Mature: 5.16

Prosite motif: PS01234 GATB

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
2.9 %Met     (Translated Protein)
3.4 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
2.9 %Met     (Mature Protein)
3.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNFETVIGLEVHVELKTNSKIFSSAPAHFGAEPNTNTTVVDLGMPGVLPVLNKRAVEFGM
CCCCEEEEEEEEEEEECCCCHHHCCCCCCCCCCCCCCEEEECCCCCHHHHHHHHHHHHCC
KAAMAINCEIAKHTKFDRKNYFYPDNPKAYQISQFDKPIGEHGWIEIEVGGKKKKIGITR
CEEEEEEEEEHHCCCCCCCCCCCCCCCCEEEHHHHCCCCCCCCEEEEEECCCEEEECEEE
LHLEEDAGKNTHTSHGYSLVDINRQGTPLIEIVSEPDIRSAEEAYAYLEKLKSIIQYTGV
EEEECCCCCCCCCCCCEEEEEECCCCCCEEEEECCCCCCCHHHHHHHHHHHHHHHHHCCC
SDVKMEEGSMRCDANISIRPIGQEEFGVKTELKNLNSFNNVRKGIEYEEKRQAEVLKSGG
CCEEECCCCEEECCCEEEEECCCHHCCCHHHHHHHHHHHHHHHCCCHHHHHHHHHHHCCC
IIEQETRRFEEATGKTSLMRIKEGSDDYRYFPEPDLVDLFIDDAWKERIRAEIPELPDKR
CCHHHHHHHHHHCCCEEEEEEECCCCCCCCCCCCCEEEEEHHHHHHHHHHHHCCCCCCCC
QIRYINDLGLPAYDAMVLTLTKEMSDFFEATLAAGADAKQASNWLMGEVSAYLNAEQKEL
CEEEHHHCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHCCHHHHH
HETGLTPENLAGMIKLIEAGTISSKIAKKVFRELAQNGGDAEQVVKDKGLVQISDEGALR
HHCCCCHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHCCCCHHHHHHCCCCEEECCCCHHH
TIISEILDNNEQSIVDFKNGKDRAVGFLVGQVMKATKGQANPPMVNKLLLEEMNKR
HHHHHHHCCCCCEEEEECCCCCHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHCCC
>Mature Secondary Structure
MNFETVIGLEVHVELKTNSKIFSSAPAHFGAEPNTNTTVVDLGMPGVLPVLNKRAVEFGM
CCCCEEEEEEEEEEEECCCCHHHCCCCCCCCCCCCCCEEEECCCCCHHHHHHHHHHHHCC
KAAMAINCEIAKHTKFDRKNYFYPDNPKAYQISQFDKPIGEHGWIEIEVGGKKKKIGITR
CEEEEEEEEEHHCCCCCCCCCCCCCCCCEEEHHHHCCCCCCCCEEEEEECCCEEEECEEE
LHLEEDAGKNTHTSHGYSLVDINRQGTPLIEIVSEPDIRSAEEAYAYLEKLKSIIQYTGV
EEEECCCCCCCCCCCCEEEEEECCCCCCEEEEECCCCCCCHHHHHHHHHHHHHHHHHCCC
SDVKMEEGSMRCDANISIRPIGQEEFGVKTELKNLNSFNNVRKGIEYEEKRQAEVLKSGG
CCEEECCCCEEECCCEEEEECCCHHCCCHHHHHHHHHHHHHHHCCCHHHHHHHHHHHCCC
IIEQETRRFEEATGKTSLMRIKEGSDDYRYFPEPDLVDLFIDDAWKERIRAEIPELPDKR
CCHHHHHHHHHHCCCEEEEEEECCCCCCCCCCCCCEEEEEHHHHHHHHHHHHCCCCCCCC
QIRYINDLGLPAYDAMVLTLTKEMSDFFEATLAAGADAKQASNWLMGEVSAYLNAEQKEL
CEEEHHHCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHCCHHHHH
HETGLTPENLAGMIKLIEAGTISSKIAKKVFRELAQNGGDAEQVVKDKGLVQISDEGALR
HHCCCCHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHCCCCHHHHHHCCCCEEECCCCHHH
TIISEILDNNEQSIVDFKNGKDRAVGFLVGQVMKATKGQANPPMVNKLLLEEMNKR
HHHHHHHCCCCCEEEEECCCCCHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11679669 [H]