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The map label for this gene is dagK [H]

Identifier: 226224356

GI number: 226224356

Start: 1808851

End: 1809783

Strand: Reverse

Name: dagK [H]

Synonym: Lm4b_01767

Alternate gene names: 226224356

Gene position: 1809783-1808851 (Counterclockwise)

Preceding gene: 226224357

Following gene: 226224355

Centisome position: 62.13

GC content: 38.16

Gene sequence:

>933_bases
ATGCAAAAACACGCTCGAGTTATTTATAATCCCACATCTGGAAGAGAAATCATCAAGAAAAATCTTGCAGATGTCCTTTC
GATATTAGAGCAAGCAGGCTATGTAACATCTGCGCACGCAACAACTGCAGAACCAGATGATGCCAAACATGCTGCGGAAG
AAGCTGTAAGAGATCGATTCGATTTAGTTGTAGCAGCTGGCGGAGATGGAACCATTAATGAAGTTATTAACGGTATTGCT
GAAAAAGAATATCGCCCCAAAGTAGGGATTATACCAACCGGGACAACCAATGATTTTGCAAGAGCCTTACATGTGCCTAG
AGACGTGATTAAAGCAACGAAGATTATAGCAGCTGGTCAAAGTGTAGCAATGGATATAGGTAAAGCAAATGAAACATATT
TTATTAATATAGGTGGCGGAGGTCGTTTAACAGAACTCACTTACGATGTTCCTAGCCGTCTAAAAACAATGCTCGGTCAA
CTTGCTTATTATTTAAAAGGTATCGAAATGTTACCATCCTTAAAAGCAACCAAAGTAAAAGTAGAATATGATCAAGGCGT
ATTTGAAGGGGAAGTGATGTTTTTCCTATTAGGATTAACTAATTCCATTGGTGGCTTTGAAAAAATCGCCCCAGATGCCA
AGTTAGACGACGGTAAATTTTCACTCATTATCGTGAAAAAAGTAAATTTAGCGGAATTTATCCGTTTAGTCACACTGGCG
CTTCGAGGAGATCATATTAAAGAACCTAATGTTATTTATGTAAAATCCGAAAAAGTGATTGTTAACTCAGAAGATAAGAT
GTTAATTAACCTTGACGGGGAGCTTGGTGGGGAAACGCCAATGGAATTCCGCAATTTAAGACAACATATCGAATTTTTCG
CAAGTGTAGATGACATCCCCGCAACTGATTTATTCATAAAAGAAAATAGCTAA

Upstream 100 bases:

>100_bases
TAGGAATTTATAGTTAAATTTGCTATAATATTACTATCATGTGTCAAAACGCGCTTTTCTTTTGAACAACGCTGTTTGAG
TGAAGGAGAGGACGAACATA

Downstream 100 bases:

>100_bases
AAACTGCCCAGTTTGGTAGTTTTTTTCTTTAGCCTAAACGTCCTAATTGCAACATAGTAAAAAGGATGTAATTATTGTTA
GAAATTTGTCTTATTTGAGC

Product: putative lipid kinase

Products: NA

Alternate protein names: DAG kinase; DAGK [H]

Number of amino acids: Translated: 310; Mature: 310

Protein sequence:

>310_residues
MQKHARVIYNPTSGREIIKKNLADVLSILEQAGYVTSAHATTAEPDDAKHAAEEAVRDRFDLVVAAGGDGTINEVINGIA
EKEYRPKVGIIPTGTTNDFARALHVPRDVIKATKIIAAGQSVAMDIGKANETYFINIGGGGRLTELTYDVPSRLKTMLGQ
LAYYLKGIEMLPSLKATKVKVEYDQGVFEGEVMFFLLGLTNSIGGFEKIAPDAKLDDGKFSLIIVKKVNLAEFIRLVTLA
LRGDHIKEPNVIYVKSEKVIVNSEDKMLINLDGELGGETPMEFRNLRQHIEFFASVDDIPATDLFIKENS

Sequences:

>Translated_310_residues
MQKHARVIYNPTSGREIIKKNLADVLSILEQAGYVTSAHATTAEPDDAKHAAEEAVRDRFDLVVAAGGDGTINEVINGIA
EKEYRPKVGIIPTGTTNDFARALHVPRDVIKATKIIAAGQSVAMDIGKANETYFINIGGGGRLTELTYDVPSRLKTMLGQ
LAYYLKGIEMLPSLKATKVKVEYDQGVFEGEVMFFLLGLTNSIGGFEKIAPDAKLDDGKFSLIIVKKVNLAEFIRLVTLA
LRGDHIKEPNVIYVKSEKVIVNSEDKMLINLDGELGGETPMEFRNLRQHIEFFASVDDIPATDLFIKENS
>Mature_310_residues
MQKHARVIYNPTSGREIIKKNLADVLSILEQAGYVTSAHATTAEPDDAKHAAEEAVRDRFDLVVAAGGDGTINEVINGIA
EKEYRPKVGIIPTGTTNDFARALHVPRDVIKATKIIAAGQSVAMDIGKANETYFINIGGGGRLTELTYDVPSRLKTMLGQ
LAYYLKGIEMLPSLKATKVKVEYDQGVFEGEVMFFLLGLTNSIGGFEKIAPDAKLDDGKFSLIIVKKVNLAEFIRLVTLA
LRGDHIKEPNVIYVKSEKVIVNSEDKMLINLDGELGGETPMEFRNLRQHIEFFASVDDIPATDLFIKENS

Specific function: Catalyzes the phosphorylation of diacylglycerol (DAG) into phosphatidic acid. Is a key enzyme involved in the production of lipoteichoic acid by reintroducing DAG formed from the breakdown of membrane phospholipids into the phosphatidylglycerol biosynthet

COG id: COG1597

COG function: function code IR; Sphingosine kinase and enzymes related to eukaryotic diacylglycerol kinase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 DAGKc domain [H]

Homologues:

Organism=Escherichia coli, GI1788402, Length=257, Percent_Identity=31.5175097276265, Blast_Score=127, Evalue=1e-30,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR017438
- InterPro:   IPR005218
- InterPro:   IPR001206 [H]

Pfam domain/function: PF00781 DAGK_cat [H]

EC number: =2.7.1.107 [H]

Molecular weight: Translated: 34025; Mature: 34025

Theoretical pI: Translated: 5.47; Mature: 5.47

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
2.3 %Met     (Translated Protein)
2.3 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
2.3 %Met     (Mature Protein)
2.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MQKHARVIYNPTSGREIIKKNLADVLSILEQAGYVTSAHATTAEPDDAKHAAEEAVRDRF
CCCCCEEEECCCCCHHHHHHHHHHHHHHHHHCCCEEECCCCCCCCCHHHHHHHHHHHHHE
DLVVAAGGDGTINEVINGIAEKEYRPKVGIIPTGTTNDFARALHVPRDVIKATKIIAAGQ
EEEEEECCCCCHHHHHHHHHHHCCCCCCCEEECCCCHHHHHHHHCCHHHHHHHHHHHCCC
SVAMDIGKANETYFINIGGGGRLTELTYDVPSRLKTMLGQLAYYLKGIEMLPSLKATKVK
HHEEECCCCCCEEEEEECCCCEEEEEEECHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEE
VEYDQGVFEGEVMFFLLGLTNSIGGFEKIAPDAKLDDGKFSLIIVKKVNLAEFIRLVTLA
EEECCCCCCCHHHHHEEEHHCCCCCHHHHCCCCCCCCCCEEEEEEEECCHHHHHHHHHHH
LRGDHIKEPNVIYVKSEKVIVNSEDKMLINLDGELGGETPMEFRNLRQHIEFFASVDDIP
HCCCCCCCCCEEEEECCEEEEECCCCEEEEECCCCCCCCCHHHHHHHHHHHHHHCCCCCC
ATDLFIKENS
CCEEEEECCC
>Mature Secondary Structure
MQKHARVIYNPTSGREIIKKNLADVLSILEQAGYVTSAHATTAEPDDAKHAAEEAVRDRF
CCCCCEEEECCCCCHHHHHHHHHHHHHHHHHCCCEEECCCCCCCCCHHHHHHHHHHHHHE
DLVVAAGGDGTINEVINGIAEKEYRPKVGIIPTGTTNDFARALHVPRDVIKATKIIAAGQ
EEEEEECCCCCHHHHHHHHHHHCCCCCCCEEECCCCHHHHHHHHCCHHHHHHHHHHHCCC
SVAMDIGKANETYFINIGGGGRLTELTYDVPSRLKTMLGQLAYYLKGIEMLPSLKATKVK
HHEEECCCCCCEEEEEECCCCEEEEEEECHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEE
VEYDQGVFEGEVMFFLLGLTNSIGGFEKIAPDAKLDDGKFSLIIVKKVNLAEFIRLVTLA
EEECCCCCCCHHHHHEEEHHCCCCCHHHHCCCCCCCCCCEEEEEEEECCHHHHHHHHHHH
LRGDHIKEPNVIYVKSEKVIVNSEDKMLINLDGELGGETPMEFRNLRQHIEFFASVDDIP
HCCCCCCCCCEEEEECCEEEEECCCCEEEEECCCCCCCCCHHHHHHHHHHHHHHCCCCCC
ATDLFIKENS
CCEEEEECCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9384377 [H]