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The map label for this gene is purR [H]

Identifier: 226224330

GI number: 226224330

Start: 1775240

End: 1776205

Strand: Reverse

Name: purR [H]

Synonym: Lm4b_01741

Alternate gene names: 226224330

Gene position: 1776205-1775240 (Counterclockwise)

Preceding gene: 226224331

Following gene: 226224329

Centisome position: 60.98

GC content: 40.79

Gene sequence:

>966_bases
GTGGTAGGAATTAAAGATATCGCGAAAAAGGCAGGGGTTTCCATTTCAACAGTATCCTACGCGCTTAATGGCAGCCCGAA
AGTGACCGAAAAAACGCGAAAACGAATTATGACCATCGCAAATGAATTGAATTATATTCCCAATATGGCAGCACGAACGT
TAAAAACAAGAGAAACAAAAATTATCGGCGTCTATTTAACGAATTACGGCGGGATTTTTTACGGGACTTTACTGGAAGGT
TTAACGCAAACGCTATATAAACATGGATATGAACTCATCGCTTGCAGTGGCACGAAAACACACCGATTTTTACCCGAGAA
AATGATTGATGGCGCTATTATTTTAGATGCGAATTTCCCGTCTAGCGAAATCATTAATTATGCTAATCGTGGTCATAAAT
TAGTGGTGCTTGACCGCGAACTTACCCATGAGAACGTGAGCCAAGTCCTTCTAGATAATAAAGGCGGCGCAACGCTAGCA
ATTGATTATTTATCTACTAATTTTAAAGGTGATTTCTATGTCATTACCGGTCCAGAAGATTCTTATGATGCAAGAGTCAG
GCTCGAAACCGCGAAACAAGAACTCGCTCGGTCAGGTGACAAAAAGGTGCATATTATTGAAGGAGACTTTTCCGAAGAAA
GTGGCGAAAAAGCAGCAGCCATTATCACGGAAAACTGGAAGGCGCCTGTTTCTGTATTTGCCCTAAATGATAACATGGCT
ATTGGGATGTATCGTTATTTTGCAGGGACAGATTTGGAAATCGGCAAAGACGTACGAATTATTGGCTTTGATAATACGGA
TATAAGTGAGTATTTGGTTCCACGTCTTGCTACTATCGAATACTCGAAGTACAAATGGGGCGCAGTTGCCGCAGATAAAC
TAGTGGAACTCCTTGAAGGTGGAAAAGCTGAAAACGAAATAATCTATACTACTAGAATCAGTGGGCCATCTGTTTCAAAC
AATTAG

Upstream 100 bases:

>100_bases
CGACAAAATGTTCACAAATCACGATTAAATTTTCAAAAAATAACCGTCTCTAAGGTTGTTTTGTGTATAATAAAAGAAAA
CAGAAAAGGATGCGATTCAC

Downstream 100 bases:

>100_bases
TACGCTTTTTTCCTTATTTTAAGGTATACTTAAGATAAAATGAAGGGAGCTGTTGGCTAATGAAACTTGGAATTATGGGA
ACGAACTGGATTACGGATTC

Product: transcription regulator (LacI family)

Products: NA

Alternate protein names: Pur regulon repressor; Purine nucleotide synthesis repressor [H]

Number of amino acids: Translated: 321; Mature: 321

Protein sequence:

>321_residues
MVGIKDIAKKAGVSISTVSYALNGSPKVTEKTRKRIMTIANELNYIPNMAARTLKTRETKIIGVYLTNYGGIFYGTLLEG
LTQTLYKHGYELIACSGTKTHRFLPEKMIDGAIILDANFPSSEIINYANRGHKLVVLDRELTHENVSQVLLDNKGGATLA
IDYLSTNFKGDFYVITGPEDSYDARVRLETAKQELARSGDKKVHIIEGDFSEESGEKAAAIITENWKAPVSVFALNDNMA
IGMYRYFAGTDLEIGKDVRIIGFDNTDISEYLVPRLATIEYSKYKWGAVAADKLVELLEGGKAENEIIYTTRISGPSVSN
N

Sequences:

>Translated_321_residues
MVGIKDIAKKAGVSISTVSYALNGSPKVTEKTRKRIMTIANELNYIPNMAARTLKTRETKIIGVYLTNYGGIFYGTLLEG
LTQTLYKHGYELIACSGTKTHRFLPEKMIDGAIILDANFPSSEIINYANRGHKLVVLDRELTHENVSQVLLDNKGGATLA
IDYLSTNFKGDFYVITGPEDSYDARVRLETAKQELARSGDKKVHIIEGDFSEESGEKAAAIITENWKAPVSVFALNDNMA
IGMYRYFAGTDLEIGKDVRIIGFDNTDISEYLVPRLATIEYSKYKWGAVAADKLVELLEGGKAENEIIYTTRISGPSVSN
N
>Mature_321_residues
MVGIKDIAKKAGVSISTVSYALNGSPKVTEKTRKRIMTIANELNYIPNMAARTLKTRETKIIGVYLTNYGGIFYGTLLEG
LTQTLYKHGYELIACSGTKTHRFLPEKMIDGAIILDANFPSSEIINYANRGHKLVVLDRELTHENVSQVLLDNKGGATLA
IDYLSTNFKGDFYVITGPEDSYDARVRLETAKQELARSGDKKVHIIEGDFSEESGEKAAAIITENWKAPVSVFALNDNMA
IGMYRYFAGTDLEIGKDVRIIGFDNTDISEYLVPRLATIEYSKYKWGAVAADKLVELLEGGKAENEIIYTTRISGPSVSN
N

Specific function: Is the main repressor of the genes involved in the de novo synthesis of purine nucleotides, regulating purB, purC, purEK, purF, purHD, purL, purMN and guaBA expression. PurR is allosterically activated to bind its cognate DNA by binding the purine corepre

COG id: COG1609

COG function: function code K; Transcriptional regulators

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 HTH lacI-type DNA-binding domain [H]

Homologues:

Organism=Escherichia coli, GI1790194, Length=326, Percent_Identity=30.0613496932515, Blast_Score=143, Evalue=2e-35,
Organism=Escherichia coli, GI1790369, Length=310, Percent_Identity=31.2903225806452, Blast_Score=138, Evalue=6e-34,
Organism=Escherichia coli, GI1787948, Length=319, Percent_Identity=28.8401253918495, Blast_Score=129, Evalue=3e-31,
Organism=Escherichia coli, GI1786540, Length=313, Percent_Identity=25.8785942492013, Blast_Score=113, Evalue=2e-26,
Organism=Escherichia coli, GI1789202, Length=292, Percent_Identity=28.4246575342466, Blast_Score=112, Evalue=2e-26,
Organism=Escherichia coli, GI1789068, Length=314, Percent_Identity=28.0254777070064, Blast_Score=110, Evalue=1e-25,
Organism=Escherichia coli, GI1788474, Length=290, Percent_Identity=27.5862068965517, Blast_Score=98, Evalue=6e-22,
Organism=Escherichia coli, GI1787580, Length=332, Percent_Identity=25.6024096385542, Blast_Score=95, Evalue=7e-21,
Organism=Escherichia coli, GI1786268, Length=299, Percent_Identity=24.7491638795987, Blast_Score=79, Evalue=3e-16,
Organism=Escherichia coli, GI1790689, Length=321, Percent_Identity=22.1183800623053, Blast_Score=78, Evalue=7e-16,
Organism=Escherichia coli, GI1789456, Length=334, Percent_Identity=26.0479041916168, Blast_Score=76, Evalue=3e-15,
Organism=Escherichia coli, GI1787906, Length=174, Percent_Identity=28.735632183908, Blast_Score=68, Evalue=7e-13,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000843
- InterPro:   IPR010982
- InterPro:   IPR001761 [H]

Pfam domain/function: PF00356 LacI; PF00532 Peripla_BP_1 [H]

EC number: NA

Molecular weight: Translated: 35473; Mature: 35473

Theoretical pI: Translated: 6.38; Mature: 6.38

Prosite motif: PS00356 HTH_LACI_1 ; PS50932 HTH_LACI_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
1.9 %Met     (Translated Protein)
2.2 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
1.9 %Met     (Mature Protein)
2.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MVGIKDIAKKAGVSISTVSYALNGSPKVTEKTRKRIMTIANELNYIPNMAARTLKTRETK
CCCHHHHHHHHCCEEEEEEEEECCCCCHHHHHHHHHHHHHHHHHCCCCHHHHHHHCCCEE
IIGVYLTNYGGIFYGTLLEGLTQTLYKHGYELIACSGTKTHRFLPEKMIDGAIILDANFP
EEEEEEECCCCHHHHHHHHHHHHHHHHCCCEEEEECCCCCHHCCCHHHCCCEEEEECCCC
SSEIINYANRGHKLVVLDRELTHENVSQVLLDNKGGATLAIDYLSTNFKGDFYVITGPED
HHHHHHHHCCCCEEEEEECHHCHHHHHHHEEECCCCCEEEEEEEECCCCCCEEEEECCCC
SYDARVRLETAKQELARSGDKKVHIIEGDFSEESGEKAAAIITENWKAPVSVFALNDNMA
CCCCEEEEHHHHHHHHHCCCCEEEEEECCCCCCCCCEEEEEEECCCCCCEEEEEECCCEE
IGMYRYFAGTDLEIGKDVRIIGFDNTDISEYLVPRLATIEYSKYKWGAVAADKLVELLEG
EEEEEEECCCCCCCCCEEEEEEECCCCHHHHHHHHHEEEEECCCCCCHHHHHHHHHHHHC
GKAENEIIYTTRISGPSVSNN
CCCCCEEEEEEEECCCCCCCC
>Mature Secondary Structure
MVGIKDIAKKAGVSISTVSYALNGSPKVTEKTRKRIMTIANELNYIPNMAARTLKTRETK
CCCHHHHHHHHCCEEEEEEEEECCCCCHHHHHHHHHHHHHHHHHCCCCHHHHHHHCCCEE
IIGVYLTNYGGIFYGTLLEGLTQTLYKHGYELIACSGTKTHRFLPEKMIDGAIILDANFP
EEEEEEECCCCHHHHHHHHHHHHHHHHCCCEEEEECCCCCHHCCCHHHCCCEEEEECCCC
SSEIINYANRGHKLVVLDRELTHENVSQVLLDNKGGATLAIDYLSTNFKGDFYVITGPED
HHHHHHHHCCCCEEEEEECHHCHHHHHHHEEECCCCCEEEEEEEECCCCCCEEEEECCCC
SYDARVRLETAKQELARSGDKKVHIIEGDFSEESGEKAAAIITENWKAPVSVFALNDNMA
CCCCEEEEHHHHHHHHHCCCCEEEEEECCCCCCCCCEEEEEEECCCCCCEEEEEECCCEE
IGMYRYFAGTDLEIGKDVRIIGFDNTDISEYLVPRLATIEYSKYKWGAVAADKLVELLEG
EEEEEEECCCCCCCCCEEEEEEECCCCHHHHHHHHHEEEEECCCCCCHHHHHHHHHHHHC
GKAENEIIYTTRISGPSVSNN
CCCCCEEEEEEEECCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA