Click here to switch to the map view.

The map label for this gene is ispD

Identifier: 226223697

GI number: 226223697

Start: 1115002

End: 1115715

Strand: Direct

Name: ispD

Synonym: Lm4b_01099

Alternate gene names: 226223697

Gene position: 1115002-1115715 (Clockwise)

Preceding gene: 226223695

Following gene: 226223698

Centisome position: 38.28

GC content: 34.59

Gene sequence:

>714_bases
ATGATATACGCTCAAATTTTAGCTGGAGGAAAAGGTACGCGGATGGGTAACGTTAGCATGCCAAAACAATTTCTACCTCT
AAATGGTAAACCAATTATTGTTCATACCGTTGAAAAATTTATTCTAAATACTCGATTTGATAAAATTCTTATTTCCTCAC
CAAAAGAATGGATGAACCACGCTGAGGATAATATTAAAAAGTATATTTCTGATGATCGTATTGTAGTCATTGAAGGCGGA
GAAGACCGCAATGAAACAATTATGAATGGTATTCGTTTTGTGGAAAAAACATATGGCTTAACAGATGATGATATTATCGT
GACGCATGATGCTGTTCGTCCATTTTTAACACATCGTATTATTGAAGAAAATATAGATGCTGCACTTGAAACAGGAGCAG
TAGATACTGTTATTGAAGCACTTGATACAATCGTTGAATCAAGCAATCATGAGGTGATTACAGATATTCCGGTCAGAGAT
CACATGTATCAAGGACAAACACCGCAAAGCTTCAATATGAAAAAAGTATTCAATCACTACCAAAATCTAACACCAGAGAA
AAAACAAATTCTAACAGATGCATGTAAAATTTGTTTGCTTGCTGGCGATGATGTGAAATTAGTTAAAGGTGAAATTTTCA
ACATTAAGATTACGACGCCTTATGATTTGAAAGTAGCGAATGCGATTATTCAGGAGCGGATAGCCAATGATTAA

Upstream 100 bases:

>100_bases
TTAAAATACTTCTTAGCGCAGATAGAAGCTAGTTTGTTATACAAGTTTTATGTTATAATGTAACATGTCAATATAAAAAA
CAGTTAAAGGAGATTAGAGG

Downstream 100 bases:

>100_bases
TCAAGTATACAGACTTGTGTCAGAGAGACAGTTTGAAGTTGCCAATGTGGACGAATCATTAACAGGTGATGTGGTTGTTG
TTCGACCGACTCATTTATCT

Product: 2-C-methyl-D-erythritol 4-phosphate cytidylyltransferase

Products: NA

Alternate protein names: 4-diphosphocytidyl-2C-methyl-D-erythritol synthase 2; MEP cytidylyltransferase 2; MCT 2 [H]

Number of amino acids: Translated: 237; Mature: 237

Protein sequence:

>237_residues
MIYAQILAGGKGTRMGNVSMPKQFLPLNGKPIIVHTVEKFILNTRFDKILISSPKEWMNHAEDNIKKYISDDRIVVIEGG
EDRNETIMNGIRFVEKTYGLTDDDIIVTHDAVRPFLTHRIIEENIDAALETGAVDTVIEALDTIVESSNHEVITDIPVRD
HMYQGQTPQSFNMKKVFNHYQNLTPEKKQILTDACKICLLAGDDVKLVKGEIFNIKITTPYDLKVANAIIQERIAND

Sequences:

>Translated_237_residues
MIYAQILAGGKGTRMGNVSMPKQFLPLNGKPIIVHTVEKFILNTRFDKILISSPKEWMNHAEDNIKKYISDDRIVVIEGG
EDRNETIMNGIRFVEKTYGLTDDDIIVTHDAVRPFLTHRIIEENIDAALETGAVDTVIEALDTIVESSNHEVITDIPVRD
HMYQGQTPQSFNMKKVFNHYQNLTPEKKQILTDACKICLLAGDDVKLVKGEIFNIKITTPYDLKVANAIIQERIAND
>Mature_237_residues
MIYAQILAGGKGTRMGNVSMPKQFLPLNGKPIIVHTVEKFILNTRFDKILISSPKEWMNHAEDNIKKYISDDRIVVIEGG
EDRNETIMNGIRFVEKTYGLTDDDIIVTHDAVRPFLTHRIIEENIDAALETGAVDTVIEALDTIVESSNHEVITDIPVRD
HMYQGQTPQSFNMKKVFNHYQNLTPEKKQILTDACKICLLAGDDVKLVKGEIFNIKITTPYDLKVANAIIQERIAND

Specific function: Catalyzes the formation of 4-diphosphocytidyl-2-C- methyl-D-erythritol from CTP and 2-C-methyl-D-erythritol 4- phosphate (MEP) [H]

COG id: COG1211

COG function: function code I; 4-diphosphocytidyl-2-methyl-D-erithritol synthase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the ispD family [H]

Homologues:

Organism=Homo sapiens, GI157412259, Length=240, Percent_Identity=28.75, Blast_Score=102, Evalue=4e-22,
Organism=Homo sapiens, GI157671913, Length=141, Percent_Identity=31.9148936170213, Blast_Score=75, Evalue=6e-14,
Organism=Escherichia coli, GI1789104, Length=229, Percent_Identity=26.2008733624454, Blast_Score=67, Evalue=1e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001228
- InterPro:   IPR018294 [H]

Pfam domain/function: PF01128 IspD [H]

EC number: =2.7.7.60 [H]

Molecular weight: Translated: 26793; Mature: 26793

Theoretical pI: Translated: 5.36; Mature: 5.36

Prosite motif: PS01295 ISPD

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
3.0 %Met     (Translated Protein)
3.8 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
3.0 %Met     (Mature Protein)
3.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MIYAQILAGGKGTRMGNVSMPKQFLPLNGKPIIVHTVEKFILNTRFDKILISSPKEWMNH
CEEEEEEECCCCCCCCCCCCCHHHCCCCCCEEEEHHHHHHHHHHHHHHHEECCCHHHHHH
AEDNIKKYISDDRIVVIEGGEDRNETIMNGIRFVEKTYGLTDDDIIVTHDAVRPFLTHRI
HHHHHHHHHCCCCEEEEECCCCCHHHHHHHHHHHHHHCCCCCCCEEEEHHHHHHHHHHHH
IEENIDAALETGAVDTVIEALDTIVESSNHEVITDIPVRDHMYQGQTPQSFNMKKVFNHY
HHHHHHHHHHCCHHHHHHHHHHHHHHCCCCEEEEECCCHHHHCCCCCCCCCCHHHHHHHH
QNLTPEKKQILTDACKICLLAGDDVKLVKGEIFNIKITTPYDLKVANAIIQERIAND
HCCCCHHHHHHHHHHHEEEECCCCEEEEECEEEEEEEECCCCHHHHHHHHHHHHCCC
>Mature Secondary Structure
MIYAQILAGGKGTRMGNVSMPKQFLPLNGKPIIVHTVEKFILNTRFDKILISSPKEWMNH
CEEEEEEECCCCCCCCCCCCCHHHCCCCCCEEEEHHHHHHHHHHHHHHHEECCCHHHHHH
AEDNIKKYISDDRIVVIEGGEDRNETIMNGIRFVEKTYGLTDDDIIVTHDAVRPFLTHRI
HHHHHHHHHCCCCEEEEECCCCCHHHHHHHHHHHHHHCCCCCCCEEEEHHHHHHHHHHHH
IEENIDAALETGAVDTVIEALDTIVESSNHEVITDIPVRDHMYQGQTPQSFNMKKVFNHY
HHHHHHHHHHCCHHHHHHHHHHHHHHCCCCEEEEECCCHHHHCCCCCCCCCCHHHHHHHH
QNLTPEKKQILTDACKICLLAGDDVKLVKGEIFNIKITTPYDLKVANAIIQERIAND
HCCCCHHHHHHHHHHHEEEECCCCEEEEECEEEEEEEECCCCHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11679669 [H]