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The map label for this gene is PdhD

Identifier: 226223673

GI number: 226223673

Start: 1083861

End: 1085264

Strand: Direct

Name: PdhD

Synonym: Lm4b_01075

Alternate gene names: 226223673

Gene position: 1083861-1085264 (Clockwise)

Preceding gene: 226223672

Following gene: 226223674

Centisome position: 37.21

GC content: 42.02

Gene sequence:

>1404_bases
ATGGTAGTAGGCGATTTTCCAGAAGAAAGAGACACCATAGTCATCGGTGCAGGCCCAGGTGGGTATGTAGCCGCAATCCG
AGCTGCACAACTCGGACAAAAAGTTACCATTATTGAAAAAGAATATTACGGCGGTGTGTGCTTAAACGTGGGATGTATTC
CTTCCAAAGCACTTATCACAATCGGTCACCGTTTTAAAGAAGCTGGACACTCTGATAACATGGGTATTACAGCAGACAAC
GTGAACTTAGATTTCACAAAAGCACAAGAATGGAAAGGCGGCGTAGTTAACAAGCTTACATCAGGTGTTAAAGGCCTTCT
TAAGAAAAATAAAGTTGAAATGTTAGAAGGAGAAGCGTTCTTCGTGGATGATCATTCCTTACGTGTGATCCACCCTGACT
CCGCTCAAACTTATACATTCAACAACGTAATCATTGCAACAGGATCTCGTCCAATCGAAATCCCAGGTTTCAAATATGGT
AAACGTGTATTAAGCTCAACTGGTGCACTTGCACTAACAGAAGTTCCGAAAAAACTAGTCGTAATTGGTGGCGGATATAT
CGGAACAGAACTAGGTGGAGCATTCGCTAACCTTGGTACAGAACTAACTATCCTTGAAGGTGGTCCAGAAATCTTACCAA
CATACGAAAAAGACATGGTTTCCCTAGTTAAACGTAATCTGAAAAGCAAAAACGTTGAAATGGTTACTAAAGCACTTGCA
AAATCTGCTGAAGAAACTGAAAACGGCGTTAAAGTAACGTATGAAGCTAACGGTGAAACTAAAACTATCGAAGCTGATTA
TGTTTTAGTGACAGTAGGTCGTCGCCCAAATACAGACGAAATCGGTTTAGAACAAGCTGGCGTTAAAGTAACAGAACGCG
GTTTAGTAGAAGTTGACAAACAAGGTCGCTCGAACGTTCCTAACATTTTCGCAATTGGTGACATCGTTCCTGGTGTTCCT
CTAGCTCACAAAGCTAGCTATGAAGCAAAAATCGCTGCTGAAGCAATTGCTGGCGAAAAAGCAGAAAACGATTATACAGC
ACTTCCAGCTGTTGTTTTCAGTGACCCAGAACTTGCAACAGTTGGTTTAACAGAAAAAGAAGCAAAAGAAAAAGGCTTTG
ATGTAAAAGCTGCTAAATTCCCATTCGGCGGTAACGGTCGTGCGCTTTCTTTAGATGCACCTGAAGGATTTGTTCGTCTA
GTTACTCGTAAAGAAGATGGCCTAGTTATCGGTGCACAAGTTGCCGGAATGAACGCTTCTGATATTATTTCAGAAATCGG
CTTAGCAATCGAATCAGGTATTACAGCTGAAGACATCGCGCTTACTATCCATGCTCATCCATCCCTTGGAGAGCTTACAA
TGGAAGCAGCTGAACTTGCTTTAGGTCGTCCAATTCACATGTAA

Upstream 100 bases:

>100_bases
GACCACCGTGTAATTGACGGCGCAACTGCTCAAAAAGCAATGAACAATATTAAACGTTTATTAAACGATCCAGAATTATT
ACTAATGGAGGTGTAACAAA

Downstream 100 bases:

>100_bases
TCAACTTAAAGCGGAGGTATCCTTTATGGATATTTCCGCTTTTTATCTATATAAATTAGTGCGTGATTTCACAAAAACTC
TTTTAGTTCATTTTTTGAAA

Product: dihydrolipoamide dehydrogenase

Products: NA

Alternate protein names: Dihydrolipoamide dehydrogenase; E3 component of pyruvate complex; S complex, 50 kDa subunit [H]

Number of amino acids: Translated: 467; Mature: 467

Protein sequence:

>467_residues
MVVGDFPEERDTIVIGAGPGGYVAAIRAAQLGQKVTIIEKEYYGGVCLNVGCIPSKALITIGHRFKEAGHSDNMGITADN
VNLDFTKAQEWKGGVVNKLTSGVKGLLKKNKVEMLEGEAFFVDDHSLRVIHPDSAQTYTFNNVIIATGSRPIEIPGFKYG
KRVLSSTGALALTEVPKKLVVIGGGYIGTELGGAFANLGTELTILEGGPEILPTYEKDMVSLVKRNLKSKNVEMVTKALA
KSAEETENGVKVTYEANGETKTIEADYVLVTVGRRPNTDEIGLEQAGVKVTERGLVEVDKQGRSNVPNIFAIGDIVPGVP
LAHKASYEAKIAAEAIAGEKAENDYTALPAVVFSDPELATVGLTEKEAKEKGFDVKAAKFPFGGNGRALSLDAPEGFVRL
VTRKEDGLVIGAQVAGMNASDIISEIGLAIESGITAEDIALTIHAHPSLGELTMEAAELALGRPIHM

Sequences:

>Translated_467_residues
MVVGDFPEERDTIVIGAGPGGYVAAIRAAQLGQKVTIIEKEYYGGVCLNVGCIPSKALITIGHRFKEAGHSDNMGITADN
VNLDFTKAQEWKGGVVNKLTSGVKGLLKKNKVEMLEGEAFFVDDHSLRVIHPDSAQTYTFNNVIIATGSRPIEIPGFKYG
KRVLSSTGALALTEVPKKLVVIGGGYIGTELGGAFANLGTELTILEGGPEILPTYEKDMVSLVKRNLKSKNVEMVTKALA
KSAEETENGVKVTYEANGETKTIEADYVLVTVGRRPNTDEIGLEQAGVKVTERGLVEVDKQGRSNVPNIFAIGDIVPGVP
LAHKASYEAKIAAEAIAGEKAENDYTALPAVVFSDPELATVGLTEKEAKEKGFDVKAAKFPFGGNGRALSLDAPEGFVRL
VTRKEDGLVIGAQVAGMNASDIISEIGLAIESGITAEDIALTIHAHPSLGELTMEAAELALGRPIHM
>Mature_467_residues
MVVGDFPEERDTIVIGAGPGGYVAAIRAAQLGQKVTIIEKEYYGGVCLNVGCIPSKALITIGHRFKEAGHSDNMGITADN
VNLDFTKAQEWKGGVVNKLTSGVKGLLKKNKVEMLEGEAFFVDDHSLRVIHPDSAQTYTFNNVIIATGSRPIEIPGFKYG
KRVLSSTGALALTEVPKKLVVIGGGYIGTELGGAFANLGTELTILEGGPEILPTYEKDMVSLVKRNLKSKNVEMVTKALA
KSAEETENGVKVTYEANGETKTIEADYVLVTVGRRPNTDEIGLEQAGVKVTERGLVEVDKQGRSNVPNIFAIGDIVPGVP
LAHKASYEAKIAAEAIAGEKAENDYTALPAVVFSDPELATVGLTEKEAKEKGFDVKAAKFPFGGNGRALSLDAPEGFVRL
VTRKEDGLVIGAQVAGMNASDIISEIGLAIESGITAEDIALTIHAHPSLGELTMEAAELALGRPIHM

Specific function: Catalyzes the oxidation of dihydrolipoamide to lipoamide [H]

COG id: COG1249

COG function: function code C; Pyruvate/2-oxoglutarate dehydrogenase complex, dihydrolipoamide dehydrogenase (E3) component, and related enzymes

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-I pyridine nucleotide-disulfide oxidoreductase family [H]

Homologues:

Organism=Homo sapiens, GI91199540, Length=463, Percent_Identity=40.6047516198704, Blast_Score=310, Evalue=1e-84,
Organism=Homo sapiens, GI50301238, Length=471, Percent_Identity=28.4501061571125, Blast_Score=176, Evalue=4e-44,
Organism=Homo sapiens, GI22035672, Length=460, Percent_Identity=29.3478260869565, Blast_Score=151, Evalue=1e-36,
Organism=Homo sapiens, GI148277065, Length=454, Percent_Identity=27.3127753303965, Blast_Score=151, Evalue=1e-36,
Organism=Homo sapiens, GI33519430, Length=454, Percent_Identity=27.3127753303965, Blast_Score=151, Evalue=1e-36,
Organism=Homo sapiens, GI33519428, Length=454, Percent_Identity=27.3127753303965, Blast_Score=151, Evalue=1e-36,
Organism=Homo sapiens, GI33519426, Length=454, Percent_Identity=27.3127753303965, Blast_Score=151, Evalue=1e-36,
Organism=Homo sapiens, GI148277071, Length=454, Percent_Identity=27.3127753303965, Blast_Score=150, Evalue=2e-36,
Organism=Homo sapiens, GI291045266, Length=456, Percent_Identity=26.9736842105263, Blast_Score=140, Evalue=2e-33,
Organism=Homo sapiens, GI291045268, Length=451, Percent_Identity=25.7206208425721, Blast_Score=121, Evalue=1e-27,
Organism=Escherichia coli, GI1786307, Length=450, Percent_Identity=43.3333333333333, Blast_Score=352, Evalue=2e-98,
Organism=Escherichia coli, GI87081717, Length=446, Percent_Identity=29.8206278026906, Blast_Score=205, Evalue=6e-54,
Organism=Escherichia coli, GI1789915, Length=435, Percent_Identity=30.1149425287356, Blast_Score=189, Evalue=2e-49,
Organism=Escherichia coli, GI87082354, Length=465, Percent_Identity=27.5268817204301, Blast_Score=171, Evalue=9e-44,
Organism=Escherichia coli, GI1789065, Length=248, Percent_Identity=25.4032258064516, Blast_Score=62, Evalue=7e-11,
Organism=Caenorhabditis elegans, GI32565766, Length=458, Percent_Identity=41.4847161572052, Blast_Score=315, Evalue=4e-86,
Organism=Caenorhabditis elegans, GI17557007, Length=483, Percent_Identity=30.2277432712215, Blast_Score=164, Evalue=9e-41,
Organism=Caenorhabditis elegans, GI71983429, Length=434, Percent_Identity=27.1889400921659, Blast_Score=137, Evalue=1e-32,
Organism=Caenorhabditis elegans, GI71983419, Length=434, Percent_Identity=27.1889400921659, Blast_Score=137, Evalue=1e-32,
Organism=Caenorhabditis elegans, GI71982272, Length=489, Percent_Identity=23.721881390593, Blast_Score=105, Evalue=6e-23,
Organism=Caenorhabditis elegans, GI17559934, Length=219, Percent_Identity=26.9406392694064, Blast_Score=79, Evalue=4e-15,
Organism=Saccharomyces cerevisiae, GI6321091, Length=474, Percent_Identity=37.9746835443038, Blast_Score=296, Evalue=6e-81,
Organism=Saccharomyces cerevisiae, GI6325166, Length=460, Percent_Identity=30.2173913043478, Blast_Score=180, Evalue=6e-46,
Organism=Saccharomyces cerevisiae, GI6325240, Length=481, Percent_Identity=27.8586278586279, Blast_Score=176, Evalue=8e-45,
Organism=Drosophila melanogaster, GI21358499, Length=476, Percent_Identity=42.2268907563025, Blast_Score=319, Evalue=2e-87,
Organism=Drosophila melanogaster, GI24640549, Length=475, Percent_Identity=30.3157894736842, Blast_Score=157, Evalue=2e-38,
Organism=Drosophila melanogaster, GI24640553, Length=475, Percent_Identity=30.3157894736842, Blast_Score=157, Evalue=2e-38,
Organism=Drosophila melanogaster, GI24640551, Length=475, Percent_Identity=30.3157894736842, Blast_Score=156, Evalue=2e-38,
Organism=Drosophila melanogaster, GI17737741, Length=477, Percent_Identity=26.8343815513627, Blast_Score=130, Evalue=2e-30,

Paralogues:

None

Copy number: 380 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 160 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 1880 Molecules/Cell In: Stationary Phase, Rich Media (Based on E. coli). 6,000 Molecules/Cell In: Glucose minimal

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR016156
- InterPro:   IPR013027
- InterPro:   IPR006258
- InterPro:   IPR004099
- InterPro:   IPR012999
- InterPro:   IPR001327 [H]

Pfam domain/function: PF00070 Pyr_redox; PF07992 Pyr_redox_2; PF02852 Pyr_redox_dim [H]

EC number: =1.8.1.4 [H]

Molecular weight: Translated: 49482; Mature: 49482

Theoretical pI: Translated: 5.01; Mature: 5.01

Prosite motif: PS00076 PYRIDINE_REDOX_1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
1.7 %Met     (Translated Protein)
2.1 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
1.7 %Met     (Mature Protein)
2.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MVVGDFPEERDTIVIGAGPGGYVAAIRAAQLGQKVTIIEKEYYGGVCLNVGCIPSKALIT
CCCCCCCCCCCEEEEECCCCCHHHHHHHHHCCCEEEEEEECCCCCEEEEECCCCCCCEEE
IGHRFKEAGHSDNMGITADNVNLDFTKAQEWKGGVVNKLTSGVKGLLKKNKVEMLEGEAF
EHHHHHHCCCCCCCCEEECCCEEEEECCCCCCCHHHHHHHHHHHHHHHCCCEEEECCCEE
FVDDHSLRVIHPDSAQTYTFNNVIIATGSRPIEIPGFKYGKRVLSSTGALALTEVPKKLV
EEECCEEEEECCCCCCEEEECEEEEEECCCEEECCCCHHHHHHHHCCCCEEEECCCCEEE
VIGGGYIGTELGGAFANLGTELTILEGGPEILPTYEKDMVSLVKRNLKSKNVEMVTKALA
EEECCEECHHHCCHHHHCCCEEEEEECCCCCCCCHHHHHHHHHHHHHCCCCHHHHHHHHH
KSAEETENGVKVTYEANGETKTIEADYVLVTVGRRPNTDEIGLEQAGVKVTERGLVEVDK
HHHHHCCCCEEEEEECCCCEEEEEEEEEEEEECCCCCCCCCCHHHCCCEEECCCCEEECC
QGRSNVPNIFAIGDIVPGVPLAHKASYEAKIAAEAIAGEKAENDYTALPAVVFSDPELAT
CCCCCCCCEEEECCCCCCCCCCCCCCCCHHHHHHHHCCCCCCCCCEECCEEEECCCCEEE
VGLTEKEAKEKGFDVKAAKFPFGGNGRALSLDAPEGFVRLVTRKEDGLVIGAQVAGMNAS
ECCCHHHHHHCCCCEEEEECCCCCCCCEEEECCCCCEEEEEEECCCCEEEEEEECCCCHH
DIISEIGLAIESGITAEDIALTIHAHPSLGELTMEAAELALGRPIHM
HHHHHHHHHHHCCCCCCCEEEEEECCCCCCHHHHHHHHHHCCCCCCC
>Mature Secondary Structure
MVVGDFPEERDTIVIGAGPGGYVAAIRAAQLGQKVTIIEKEYYGGVCLNVGCIPSKALIT
CCCCCCCCCCCEEEEECCCCCHHHHHHHHHCCCEEEEEEECCCCCEEEEECCCCCCCEEE
IGHRFKEAGHSDNMGITADNVNLDFTKAQEWKGGVVNKLTSGVKGLLKKNKVEMLEGEAF
EHHHHHHCCCCCCCCEEECCCEEEEECCCCCCCHHHHHHHHHHHHHHHCCCEEEECCCEE
FVDDHSLRVIHPDSAQTYTFNNVIIATGSRPIEIPGFKYGKRVLSSTGALALTEVPKKLV
EEECCEEEEECCCCCCEEEECEEEEEECCCEEECCCCHHHHHHHHCCCCEEEECCCCEEE
VIGGGYIGTELGGAFANLGTELTILEGGPEILPTYEKDMVSLVKRNLKSKNVEMVTKALA
EEECCEECHHHCCHHHHCCCEEEEEECCCCCCCCHHHHHHHHHHHHHCCCCHHHHHHHHH
KSAEETENGVKVTYEANGETKTIEADYVLVTVGRRPNTDEIGLEQAGVKVTERGLVEVDK
HHHHHCCCCEEEEEECCCCEEEEEEEEEEEEECCCCCCCCCCHHHCCCEEECCCCEEECC
QGRSNVPNIFAIGDIVPGVPLAHKASYEAKIAAEAIAGEKAENDYTALPAVVFSDPELAT
CCCCCCCCEEEECCCCCCCCCCCCCCCCHHHHHHHHCCCCCCCCCEECCEEEECCCCEEE
VGLTEKEAKEKGFDVKAAKFPFGGNGRALSLDAPEGFVRLVTRKEDGLVIGAQVAGMNAS
ECCCHHHHHHCCCCEEEEECCCCCCCCEEEECCCCCEEEEEEECCCCEEEEEEECCCCHH
DIISEIGLAIESGITAEDIALTIHAHPSLGELTMEAAELALGRPIHM
HHHHHHHHHHHCCCCCCCEEEEEECCCCCCHHHHHHHHHHCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 1697575; 8969500; 9384377; 1936936 [H]