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The map label for this gene is moaA

Identifier: 226223665

GI number: 226223665

Start: 1075362

End: 1076363

Strand: Direct

Name: moaA

Synonym: Lm4b_01067

Alternate gene names: 226223665

Gene position: 1075362-1076363 (Clockwise)

Preceding gene: 226223664

Following gene: 226223668

Centisome position: 36.92

GC content: 38.02

Gene sequence:

>1002_bases
ATGCAATTATTAAAGGATAAATTTGGGCGGGTACACGATTATATTCGTATTTCAGTAACAGATCGGTGTAATTTAAGGTG
TGTGTATTGTATGCCCGAAGAAGGCCTGACATTTTTGCCTCATGAAAAAGTACTATCCAAAGATGAAATTGTCAGCTTTA
TGGAATTAATGGTGAAATTCGGCATCAAAAAAGTCCGCATCACTGGCGGAGAGCCGTTACTAAGAACTGATATAGTGGAA
ATTGTTCGCGGGCTGGGAGCAATTCCTGAAATAGAGGATATTTCGATTACAACGAATGCTATGTATTTGGCAAAAAAAGC
AGAAGCACTGAAGGATGCGGGGCTCACACGTGTGAATATCAGCTTAGATTCTCTTCATGCAGATCGTTTTAAAGCAATTA
CACGAGGTGGCCGTTTGCAAAAAGTACTTGATGGTATTCAAAAAGCAGAAGAAGTTGGACTGTTCCCAATTAAGCTTAAT
GTCGTACTAATTAAAGGACAAAACGATGACGAAATAACTGATTTCCTTCGATTTACAAAAGATAAAGATATTAATATTCG
TTTCATTGAATACATGCCAATTGGTCATGCTGGTACGAGTTGGAAAGAAAAATATTTGCCGCTTGATACAATTTTTGAAG
CTTGTAATGAAGCTGGTTATGAGTATGAACCAGTGGACTCGATTCGTGGAAATGGCCCCTCCGAAAATTTCCGTATAAAA
GGAGCGAAAGGGACGTTTGGTGTGATTCACCCGGTTAGCGCTCATTTTTGTGATAGTTGCAACCGACTCAGACTTACTGC
AGATGGTTATATTAAAGCATGTTTATACTGGGATGAAGAAATGAATATCCGCCCATTTATCCAAGATCCAGTGAAATTAA
TGCAATTGGTGCAAAAAGCAATTGATAATAAGCCTGAAAATCATGAAATGGCATTAAAATTACAAGATGAAGTACAATCT
AATAAACCAACTTGGCGGCGTATGAGTCAAATTGGAGGATAA

Upstream 100 bases:

>100_bases
ATGCGGATTGAAAAAACGTATTTAGTGGAAAAAACAGGTGGAAAAAGCGGGACATTTAAAGCAGAAGCGTAACTATTTTG
TAGGATAGGAGTTTTGTTTT

Downstream 100 bases:

>100_bases
ATAAAACATCCGGTCACATGTGTGGTCGGATGTTTTATTTTTGTCGTTCTGCTATTAAATGAGGTAATTCTGGGATAATC
AATTTTTGAACAGCGAGCTG

Product: molybdenum cofactor biosynthesis protein A

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 333; Mature: 333

Protein sequence:

>333_residues
MQLLKDKFGRVHDYIRISVTDRCNLRCVYCMPEEGLTFLPHEKVLSKDEIVSFMELMVKFGIKKVRITGGEPLLRTDIVE
IVRGLGAIPEIEDISITTNAMYLAKKAEALKDAGLTRVNISLDSLHADRFKAITRGGRLQKVLDGIQKAEEVGLFPIKLN
VVLIKGQNDDEITDFLRFTKDKDINIRFIEYMPIGHAGTSWKEKYLPLDTIFEACNEAGYEYEPVDSIRGNGPSENFRIK
GAKGTFGVIHPVSAHFCDSCNRLRLTADGYIKACLYWDEEMNIRPFIQDPVKLMQLVQKAIDNKPENHEMALKLQDEVQS
NKPTWRRMSQIGG

Sequences:

>Translated_333_residues
MQLLKDKFGRVHDYIRISVTDRCNLRCVYCMPEEGLTFLPHEKVLSKDEIVSFMELMVKFGIKKVRITGGEPLLRTDIVE
IVRGLGAIPEIEDISITTNAMYLAKKAEALKDAGLTRVNISLDSLHADRFKAITRGGRLQKVLDGIQKAEEVGLFPIKLN
VVLIKGQNDDEITDFLRFTKDKDINIRFIEYMPIGHAGTSWKEKYLPLDTIFEACNEAGYEYEPVDSIRGNGPSENFRIK
GAKGTFGVIHPVSAHFCDSCNRLRLTADGYIKACLYWDEEMNIRPFIQDPVKLMQLVQKAIDNKPENHEMALKLQDEVQS
NKPTWRRMSQIGG
>Mature_333_residues
MQLLKDKFGRVHDYIRISVTDRCNLRCVYCMPEEGLTFLPHEKVLSKDEIVSFMELMVKFGIKKVRITGGEPLLRTDIVE
IVRGLGAIPEIEDISITTNAMYLAKKAEALKDAGLTRVNISLDSLHADRFKAITRGGRLQKVLDGIQKAEEVGLFPIKLN
VVLIKGQNDDEITDFLRFTKDKDINIRFIEYMPIGHAGTSWKEKYLPLDTIFEACNEAGYEYEPVDSIRGNGPSENFRIK
GAKGTFGVIHPVSAHFCDSCNRLRLTADGYIKACLYWDEEMNIRPFIQDPVKLMQLVQKAIDNKPENHEMALKLQDEVQS
NKPTWRRMSQIGG

Specific function: Together with moaC, is involved in the conversion of a guanosine derivative (5'-GTP) into molybdopterin precursor Z [H]

COG id: COG2896

COG function: function code H; Molybdenum cofactor biosynthesis enzyme

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the radical SAM superfamily. MoaA family [H]

Homologues:

Organism=Homo sapiens, GI116734668, Length=335, Percent_Identity=38.8059701492537, Blast_Score=245, Evalue=4e-65,
Organism=Homo sapiens, GI22027536, Length=335, Percent_Identity=38.8059701492537, Blast_Score=245, Evalue=4e-65,
Organism=Escherichia coli, GI1786999, Length=337, Percent_Identity=32.0474777448071, Blast_Score=190, Evalue=1e-49,
Organism=Caenorhabditis elegans, GI17561210, Length=312, Percent_Identity=41.9871794871795, Blast_Score=253, Evalue=8e-68,
Organism=Caenorhabditis elegans, GI32566331, Length=337, Percent_Identity=38.8724035608309, Blast_Score=251, Evalue=3e-67,
Organism=Caenorhabditis elegans, GI32566329, Length=312, Percent_Identity=41.025641025641, Blast_Score=249, Evalue=2e-66,
Organism=Drosophila melanogaster, GI28574488, Length=338, Percent_Identity=41.4201183431953, Blast_Score=253, Evalue=1e-67,
Organism=Drosophila melanogaster, GI28574490, Length=313, Percent_Identity=42.1725239616613, Blast_Score=252, Evalue=3e-67,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013785
- InterPro:   IPR006638
- InterPro:   IPR013483
- InterPro:   IPR000385
- InterPro:   IPR010505
- InterPro:   IPR007197 [H]

Pfam domain/function: PF06463 Mob_synth_C; PF04055 Radical_SAM [H]

EC number: NA

Molecular weight: Translated: 37867; Mature: 37867

Theoretical pI: Translated: 7.14; Mature: 7.14

Prosite motif: PS01305 MOAA_NIFB_PQQE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.1 %Cys     (Translated Protein)
3.0 %Met     (Translated Protein)
5.1 %Cys+Met (Translated Protein)
2.1 %Cys     (Mature Protein)
3.0 %Met     (Mature Protein)
5.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MQLLKDKFGRVHDYIRISVTDRCNLRCVYCMPEEGLTFLPHEKVLSKDEIVSFMELMVKF
CCHHHHHHHHHEEEEEEEEECCCCEEEEEEECCCCCEECCHHHHCCHHHHHHHHHHHHHH
GIKKVRITGGEPLLRTDIVEIVRGLGAIPEIEDISITTNAMYLAKKAEALKDAGLTRVNI
CCEEEEEECCCCHHHHHHHHHHHHCCCCCCCCCEEEECHHHHHHHHHHHHHHCCCEEEEE
SLDSLHADRFKAITRGGRLQKVLDGIQKAEEVGLFPIKLNVVLIKGQNDDEITDFLRFTK
EHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHCCEEEEEEEEEEEECCCCHHHHHHHHHCC
DKDINIRFIEYMPIGHAGTSWKEKYLPLDTIFEACNEAGYEYEPVDSIRGNGPSENFRIK
CCCCEEEEEEEEECCCCCCCCHHHCCCHHHHHHHHHHCCCCCCCCHHHCCCCCCCCEEEE
GAKGTFGVIHPVSAHFCDSCNRLRLTADGYIKACLYWDEEMNIRPFIQDPVKLMQLVQKA
ECCCCCEEECCCHHHHHCCCCEEEEEECCEEEEEEEECCCCCCCCCHHHHHHHHHHHHHH
IDNKPENHEMALKLQDEVQSNKPTWRRMSQIGG
HCCCCCCCEEEEEEHHHHCCCCCHHHHHHHHCC
>Mature Secondary Structure
MQLLKDKFGRVHDYIRISVTDRCNLRCVYCMPEEGLTFLPHEKVLSKDEIVSFMELMVKF
CCHHHHHHHHHEEEEEEEEECCCCEEEEEEECCCCCEECCHHHHCCHHHHHHHHHHHHHH
GIKKVRITGGEPLLRTDIVEIVRGLGAIPEIEDISITTNAMYLAKKAEALKDAGLTRVNI
CCEEEEEECCCCHHHHHHHHHHHHCCCCCCCCCEEEECHHHHHHHHHHHHHHCCCEEEEE
SLDSLHADRFKAITRGGRLQKVLDGIQKAEEVGLFPIKLNVVLIKGQNDDEITDFLRFTK
EHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHCCEEEEEEEEEEEECCCCHHHHHHHHHCC
DKDINIRFIEYMPIGHAGTSWKEKYLPLDTIFEACNEAGYEYEPVDSIRGNGPSENFRIK
CCCCEEEEEEEEECCCCCCCCHHHCCCHHHHHHHHHHCCCCCCCCHHHCCCCCCCCEEEE
GAKGTFGVIHPVSAHFCDSCNRLRLTADGYIKACLYWDEEMNIRPFIQDPVKLMQLVQKA
ECCCCCEEECCCHHHHHCCCCEEEEEECCEEEEEEEECCCCCCCCCHHHHHHHHHHHHHH
IDNKPENHEMALKLQDEVQSNKPTWRRMSQIGG
HCCCCCCCEEEEEEHHHHCCCCCHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: Fe [C]

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11679669 [H]