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The map label for this gene is liaS [H]

Identifier: 226223639

GI number: 226223639

Start: 1051034

End: 1052092

Strand: Direct

Name: liaS [H]

Synonym: Lm4b_01041

Alternate gene names: 226223639

Gene position: 1051034-1052092 (Clockwise)

Preceding gene: 226223638

Following gene: 226223640

Centisome position: 36.08

GC content: 38.9

Gene sequence:

>1059_bases
ATGAGTTTTTCGAAACTGATGATGGCTGTTTGCTCAGGGTTACTACTTGTTGCTACAGCCATCGGTACGGTCGGTTACTA
TTTTTTTAGTGAAGGTTCTTGGTATAAAATTCTCATCGAACAAAAAATCTTTTACATTCCCTTTGTGGTTTTCGTTCCGC
TTACAGCCATTTCGATTGGTTTAATTATAGGCGGTTTGCTCGGGTATTTTGTTAAACAAAAATTTGAATCAATTGACTTC
TCGATTCGGCTGCTCGGACAAGGCGATTTGGAGAAAAAGATTACGAGTGAAGAAGACGAGAACTTTTTAGAAGTGCAGCA
AGTATACAAGCAAATCGACCGCTTACGTGATAAAATGAAAGCACAAACGATTTTGACACAAAAACTTGCAAGCGACCGTG
CTGAATCAACTGGCCAAACGAAAGAAGCTATTTTGTCAGAAGAGAGACACCGGATTGCTAGAGAATTACACGATTCAGTG
AGCCAACAGCTTTTTGCAGCAATGATGCTTTTATCAGCTTTGAATGAACAGAGTGAAAAAAGCGCAACACCGGCGATGCA
AAAACAACTAAAAATGGTTGAATCTATCGTAAATGAATCCCAATCAGAGATGCGGGCGCTTTTACTTCATCTGCGTCCAA
CGCAACTCGAGGGTAAATCATTAAAAACTGGTATTGAGCAATTATTAAAAGAATTAACAACCAAATTGCCAATCGAAGTA
GAATGGCAAATCGAGGATATTAGTTTGCAAAAAGGCATTGAGGATCATTTGTTCCGTATTGTTCAAGAATTACTTTCCAA
TACATTACGACATTCTAAAGCTAAATTATTAGAAGTACGCCTCGTAACGATGGATAATTTAGCGGTGATGAAAGTAGTTG
ATGACGGCGTTGGGTTTGATATGGATAATGTTCGACAAGGCTCTTATGGATTACAAAATATGCGAGAACGAGTTGCTGAA
TTCGGCGGCACCATCAAAATAATTAGTTTCCCAGGAAGAGGAACTAGTGTAGAAATAAAAATCCCACTTGTTGCGAAAAA
GGACGTGGAAAGCGAATGA

Upstream 100 bases:

>100_bases
ATAATAGCATCAAGGTCTACTCAGATAATTACGAAAGTTCCGCACGAAAAGTGAAAATCATGACTTCTGTAATCTTCGGA
GATTTAGAGGTGATTAGACT

Downstream 100 bases:

>100_bases
TAAAAGTATTACTTGTAGATGATCATGAAATGGTACGTATAGGTGTCTCAGCTTATCTTTCTGTGCAAGATGACATGGAA
GTGGTTGGCGAGGCAGAGAA

Product: two-component sensor histidine kinase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 352; Mature: 351

Protein sequence:

>352_residues
MSFSKLMMAVCSGLLLVATAIGTVGYYFFSEGSWYKILIEQKIFYIPFVVFVPLTAISIGLIIGGLLGYFVKQKFESIDF
SIRLLGQGDLEKKITSEEDENFLEVQQVYKQIDRLRDKMKAQTILTQKLASDRAESTGQTKEAILSEERHRIARELHDSV
SQQLFAAMMLLSALNEQSEKSATPAMQKQLKMVESIVNESQSEMRALLLHLRPTQLEGKSLKTGIEQLLKELTTKLPIEV
EWQIEDISLQKGIEDHLFRIVQELLSNTLRHSKAKLLEVRLVTMDNLAVMKVVDDGVGFDMDNVRQGSYGLQNMRERVAE
FGGTIKIISFPGRGTSVEIKIPLVAKKDVESE

Sequences:

>Translated_352_residues
MSFSKLMMAVCSGLLLVATAIGTVGYYFFSEGSWYKILIEQKIFYIPFVVFVPLTAISIGLIIGGLLGYFVKQKFESIDF
SIRLLGQGDLEKKITSEEDENFLEVQQVYKQIDRLRDKMKAQTILTQKLASDRAESTGQTKEAILSEERHRIARELHDSV
SQQLFAAMMLLSALNEQSEKSATPAMQKQLKMVESIVNESQSEMRALLLHLRPTQLEGKSLKTGIEQLLKELTTKLPIEV
EWQIEDISLQKGIEDHLFRIVQELLSNTLRHSKAKLLEVRLVTMDNLAVMKVVDDGVGFDMDNVRQGSYGLQNMRERVAE
FGGTIKIISFPGRGTSVEIKIPLVAKKDVESE
>Mature_351_residues
SFSKLMMAVCSGLLLVATAIGTVGYYFFSEGSWYKILIEQKIFYIPFVVFVPLTAISIGLIIGGLLGYFVKQKFESIDFS
IRLLGQGDLEKKITSEEDENFLEVQQVYKQIDRLRDKMKAQTILTQKLASDRAESTGQTKEAILSEERHRIARELHDSVS
QQLFAAMMLLSALNEQSEKSATPAMQKQLKMVESIVNESQSEMRALLLHLRPTQLEGKSLKTGIEQLLKELTTKLPIEVE
WQIEDISLQKGIEDHLFRIVQELLSNTLRHSKAKLLEVRLVTMDNLAVMKVVDDGVGFDMDNVRQGSYGLQNMRERVAEF
GGTIKIISFPGRGTSVEIKIPLVAKKDVESE

Specific function: Member of the two-component regulatory system liaS/liaR probably involved in response to a subset of cell wall-active antibiotics that interfere with the lipid II cycle in the cytoplasmic membrane (bacitracin, nisin, ramoplanin and vancomycin). Seems also

COG id: COG4585

COG function: function code T; Signal transduction histidine kinase

Gene ontology:

Cell location: Cell membrane; Multi-pass membrane protein (Potential) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 histidine kinase domain [H]

Homologues:

Organism=Escherichia coli, GI1787474, Length=232, Percent_Identity=31.8965517241379, Blast_Score=97, Evalue=2e-21,
Organism=Escherichia coli, GI1788812, Length=203, Percent_Identity=30.5418719211823, Blast_Score=82, Evalue=8e-17,
Organism=Escherichia coli, GI87082311, Length=242, Percent_Identity=27.6859504132231, Blast_Score=72, Evalue=5e-14,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003594
- InterPro:   IPR003660
- InterPro:   IPR011712
- InterPro:   IPR005467
- InterPro:   IPR017202 [H]

Pfam domain/function: PF00672 HAMP; PF02518 HATPase_c; PF07730 HisKA_3 [H]

EC number: =2.7.13.3 [H]

Molecular weight: Translated: 39739; Mature: 39608

Theoretical pI: Translated: 6.10; Mature: 6.10

Prosite motif: PS50109 HIS_KIN

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
3.7 %Met     (Translated Protein)
4.0 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
3.4 %Met     (Mature Protein)
3.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSFSKLMMAVCSGLLLVATAIGTVGYYFFSEGSWYKILIEQKIFYIPFVVFVPLTAISIG
CCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEHHHHHHHHHHHHHHHHHHHHHHH
LIIGGLLGYFVKQKFESIDFSIRLLGQGDLEKKITSEEDENFLEVQQVYKQIDRLRDKMK
HHHHHHHHHHHHHHHHCCCEEEEEECCCCHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHH
AQTILTQKLASDRAESTGQTKEAILSEERHRIARELHDSVSQQLFAAMMLLSALNEQSEK
HHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCHHHHH
SATPAMQKQLKMVESIVNESQSEMRALLLHLRPTQLEGKSLKTGIEQLLKELTTKLPIEV
CCCHHHHHHHHHHHHHHCCCHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHCCCEE
EWQIEDISLQKGIEDHLFRIVQELLSNTLRHSKAKLLEVRLVTMDNLAVMKVVDDGVGFD
EEEEEHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHEEEEEECCCCEEHHHHCCCCCC
MDNVRQGSYGLQNMRERVAEFGGTIKIISFPGRGTSVEIKIPLVAKKDVESE
HHHCCCCCCCHHHHHHHHHHHCCEEEEEEECCCCCEEEEEEEEEECCCCCCC
>Mature Secondary Structure 
SFSKLMMAVCSGLLLVATAIGTVGYYFFSEGSWYKILIEQKIFYIPFVVFVPLTAISIG
CHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEHHHHHHHHHHHHHHHHHHHHHHH
LIIGGLLGYFVKQKFESIDFSIRLLGQGDLEKKITSEEDENFLEVQQVYKQIDRLRDKMK
HHHHHHHHHHHHHHHHCCCEEEEEECCCCHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHH
AQTILTQKLASDRAESTGQTKEAILSEERHRIARELHDSVSQQLFAAMMLLSALNEQSEK
HHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCHHHHH
SATPAMQKQLKMVESIVNESQSEMRALLLHLRPTQLEGKSLKTGIEQLLKELTTKLPIEV
CCCHHHHHHHHHHHHHHCCCHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHCCCEE
EWQIEDISLQKGIEDHLFRIVQELLSNTLRHSKAKLLEVRLVTMDNLAVMKVVDDGVGFD
EEEEEHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHEEEEEECCCCEEHHHHCCCCCC
MDNVRQGSYGLQNMRERVAEFGGTIKIISFPGRGTSVEIKIPLVAKKDVESE
HHHCCCCCCCHHHHHHHHHHHCCEEEEEEECCCCCEEEEEEEEEECCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 9639930; 9384377 [H]