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The map label for this gene is gbuB

Identifier: 226223633

GI number: 226223633

Start: 1046059

End: 1046907

Strand: Direct

Name: gbuB

Synonym: Lm4b_01035

Alternate gene names: 226223633

Gene position: 1046059-1046907 (Clockwise)

Preceding gene: 226223632

Following gene: 226223634

Centisome position: 35.91

GC content: 40.05

Gene sequence:

>849_bases
ATGCCTAATATTCCAACGATTCCATTAGCTAGTTGGATTGACAAATTAGTGGATGGCTTAACGCAATTTGAAGGATTTTT
TAATGTAATTACAAATATCATTGGCGGGATTGTTGATGCATTCCAATGGGTGTTCGATTTAGTTCCACCGTGGTTATTTA
TTATTTTACTTGTATTTGGCACATTTTGGGTAAACCGTAAAGGCAAAAAATGGGGTTTAATTATCTTTGAAGTTGTCGGT
TTACTGTTAATTTGGAACTTAGATTTCTGGCGTGATATGACACAAACATTAACGCTCGTTCTGACAAGTAGTTTAATTGC
GCTTGTTATTGGGGTTCCACTTGGTATTTGGATGGCGAAAAGCAATATTGTTGAAAGTATCTTCAAACCGGTACTCGATT
TTATGCAAACTATGCCAGCCTTCGTTTACTTAATTCCTGCCGTAGCATTTTTCGGAATTGGAATGGTTCCAGGGGTTGTG
GCTTCTGTTATTTTCGCAATGCCTCCAACTGTTCGTATGACTAATCTAGGTATTCGCCAAGTTTCAACAGAGCTTGTAGA
AGCAGCCGATTCGTTCGGTTCAACACCTTGGCAAAAACTTTGGAAAGTACAGCTACCAATGGCGAAATCAACCATGATGG
CGGGTATTAACCAAAGTATCATGTTAGCACTTTCCATGGTCGTAATTGCTTCAATGATCGGTGCAATGGGACTTGGAACA
CGTGTTTACTTCGCAGTAGGACGTAACGACGCTGGTGGCGGATTTGTAGCTGGGATTGCGATTGTTATCGTAGCAATCAT
CCTAGACCGTCTGACACAAGCTTTCAATAAAAAAGCGAAATCAGAATGA

Upstream 100 bases:

>100_bases
ACAACGATTCCGATTGCTGTAACCGAAGATGGAAAATTAAAAGGAATTATCATTCGCGGATCCGTTCTGGCCGCACTTTC
TGGAAACGAGGTGAACGTTA

Downstream 100 bases:

>100_bases
GGAGGAGAGTAGATTGCTAAAAAAATTAATCACCACCGCAGTTTTGGCTATGCTTATTTTCACTTTAGCAGCCTGCGGAA
CAACCCTTGCTCCTTATGAC

Product: glycine betaine ABC transporter (permease)

Products: ADP; phosphate; L-proline [Cytoplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 282; Mature: 281

Protein sequence:

>282_residues
MPNIPTIPLASWIDKLVDGLTQFEGFFNVITNIIGGIVDAFQWVFDLVPPWLFIILLVFGTFWVNRKGKKWGLIIFEVVG
LLLIWNLDFWRDMTQTLTLVLTSSLIALVIGVPLGIWMAKSNIVESIFKPVLDFMQTMPAFVYLIPAVAFFGIGMVPGVV
ASVIFAMPPTVRMTNLGIRQVSTELVEAADSFGSTPWQKLWKVQLPMAKSTMMAGINQSIMLALSMVVIASMIGAMGLGT
RVYFAVGRNDAGGGFVAGIAIVIVAIILDRLTQAFNKKAKSE

Sequences:

>Translated_282_residues
MPNIPTIPLASWIDKLVDGLTQFEGFFNVITNIIGGIVDAFQWVFDLVPPWLFIILLVFGTFWVNRKGKKWGLIIFEVVG
LLLIWNLDFWRDMTQTLTLVLTSSLIALVIGVPLGIWMAKSNIVESIFKPVLDFMQTMPAFVYLIPAVAFFGIGMVPGVV
ASVIFAMPPTVRMTNLGIRQVSTELVEAADSFGSTPWQKLWKVQLPMAKSTMMAGINQSIMLALSMVVIASMIGAMGLGT
RVYFAVGRNDAGGGFVAGIAIVIVAIILDRLTQAFNKKAKSE
>Mature_281_residues
PNIPTIPLASWIDKLVDGLTQFEGFFNVITNIIGGIVDAFQWVFDLVPPWLFIILLVFGTFWVNRKGKKWGLIIFEVVGL
LLIWNLDFWRDMTQTLTLVLTSSLIALVIGVPLGIWMAKSNIVESIFKPVLDFMQTMPAFVYLIPAVAFFGIGMVPGVVA
SVIFAMPPTVRMTNLGIRQVSTELVEAADSFGSTPWQKLWKVQLPMAKSTMMAGINQSIMLALSMVVIASMIGAMGLGTR
VYFAVGRNDAGGGFVAGIAIVIVAIILDRLTQAFNKKAKSE

Specific function: Involved in a multicomponent binding-protein-dependent transport system for glycine betaine; probably responsible for the translocation of the substrate across the membrane [H]

COG id: COG4176

COG function: function code E; ABC-type proline/glycine betaine transport system, permease component

Gene ontology:

Cell location: Cell membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 ABC transmembrane type-1 domain [H]

Homologues:

Organism=Escherichia coli, GI1789033, Length=277, Percent_Identity=47.2924187725632, Blast_Score=213, Evalue=1e-56,
Organism=Escherichia coli, GI1788451, Length=201, Percent_Identity=30.8457711442786, Blast_Score=84, Evalue=9e-18,
Organism=Escherichia coli, GI1788449, Length=190, Percent_Identity=30.5263157894737, Blast_Score=82, Evalue=3e-17,
Organism=Escherichia coli, GI1786564, Length=155, Percent_Identity=28.3870967741935, Blast_Score=66, Evalue=3e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000515 [H]

Pfam domain/function: PF00528 BPD_transp_1 [H]

EC number: NA

Molecular weight: Translated: 30920; Mature: 30788

Theoretical pI: Translated: 9.88; Mature: 9.88

Prosite motif: PS50928 ABC_TM1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
5.3 %Met     (Translated Protein)
5.3 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
5.0 %Met     (Mature Protein)
5.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure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CEEEEECCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCC
>Mature Secondary Structure 
PNIPTIPLASWIDKLVDGLTQFEGFFNVITNIIGGIVDAFQWVFDLVPPWLFIILLVFG
CCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
TFWVNRKGKKWGLIIFEVVGLLLIWNLDFWRDMTQTLTLVLTSSLIALVIGVPLGIWMAK
HHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
SNIVESIFKPVLDFMQTMPAFVYLIPAVAFFGIGMVPGVVASVIFAMPPTVRMTNLGIRQ
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHH
VSTELVEAADSFGSTPWQKLWKVQLPMAKSTMMAGINQSIMLALSMVVIASMIGAMGLGT
HHHHHHHHHHHCCCCHHHHHHHHHCCHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHCCCC
RVYFAVGRNDAGGGFVAGIAIVIVAIILDRLTQAFNKKAKSE
CEEEEECCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: ATP; L-proline [Periplasm]; H2O [C]

Specific reaction: ATP + L-proline [Periplasm] + H2O = ADP + phosphate + L-proline [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 7622480; 8969502; 9384377 [H]