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The map label for this gene is ptsI [H]

Identifier: 226223621

GI number: 226223621

Start: 1034247

End: 1035965

Strand: Direct

Name: ptsI [H]

Synonym: Lm4b_01023

Alternate gene names: 226223621

Gene position: 1034247-1035965 (Clockwise)

Preceding gene: 226223620

Following gene: 226223622

Centisome position: 35.51

GC content: 38.45

Gene sequence:

>1719_bases
ATGGCTAAAGAGTTGAAAGGTATCGCAGCATCTGATGGGATTGCCATTGCGAAAGCTTATCTGCTCGTTGAACCTGATCT
TTCCTATGAAAAAACAGAAGTTACGGATGTTGAGAGTGAAGTAAAGCGTTTTGAAAGCGCGTTAGAAGTTTCTAGAACAG
AACTTTCAATGATTCGTGAGAAAGCTGCTAAAGACTTAGGCGAAGATAAGGCACAAATTTTTGACGCGCATCTTCTAGTG
TTAAATGATCCTGAATTAACAGGACCAATTGAAGAAAGTATCAAAAATTCTAAAACGAATGCAGAAACAGCTTTACAAGA
AACGACAGACATGTTTATTGGTATGTTTGAATCTATGGACAACGAATACATGCGTGAACGTGCAGCGGATATTAAAGATG
TACGTAAACGTGTTCTTTCTCACTTACTAGGTGTAACTATTCCTAATCCAGCTTTAATTGATGAGGAAGTAGTTGTTGTT
GCAGCTGATTTAACGCCTTCCGATACCGCACAACTTAACCGTAACTTTGTTAAAGGTTTCGTAACGGATATTGGTGGACG
TACTTCTCACTCCGCTATTATGGCACGTTCTCTTGAAATTCCAGCAGTAGTTGGAACAAAAGAAGTTACTGCTAGCGTAG
CGAAAAACGATATTGTTATTATTGATGGTTTGGAAGGTAATGTTATCATCCACCCAACAGAAGAACAAATCGCTCACTAT
GAAAAAATTAAATCTGATTTTGCTTTACAACAAGCAGAATGGGATAAACTTAAAAATGAAAAAACCGTTTCTAAAGACGG
CGTTCACGTGGAGCTTGCAGCAAACATCGGAACTCCGAATGATTTAGAAGGTGTTATTTCTAACGGTGGGGAAGCAGTTG
GACTTTATCGTACAGAATTCTTGTACATGGGTCGCGACGATTTCCCAACAGAAGAAGAGCAATTCGAAGCTTATAAAGCA
GTAGTTTCCGGAATGGACGGAAAATCTGTGGTTGTTCGTACATTAGATATCGGTGGCGATAAAACATTACCATACCTAGA
ACTTCCTGAAGAAATGAACCCATTCTTAGGATTCCGTGCAATCCGTCTTTGTTTTGCGAATGAAGAATTATTCCGTACAC
AACTTCGCGCCTTACTTCGCGCAAGTGTATATGGTAACTTAAAAATTATGTTCCCGATGATTGCAACAGTAAATGAATTC
CGTCAAGCGCGTGATATTTTACTAGATGAGAAAGCAAAACTAAAAGCTGCTGGAACAGAAGTATCTGATTCCATCGAACT
TGGAATTATGATTGAAATTCCTGCTGCTGCTGTTCTTGCTGATCAATTTGCGAAAGAAGTTGATTTCTTCTCTATCGGAA
CAAATGACTTAATTCAGTATACAATGGCTGCAGACCGTATGAATGAACGCGTTTCTTACCTTTACCAACCATACAATCCA
TCCATTTTACGTTTAGTCAAAATGGTTATCGATGCTTCTCATAAAGAGGGCAAATGGACTGGTATGTGTGGAGAAATGGC
TGGAGATCAAACGGCTGTACCACTTCTTTTAGGCCTAGGCTTAGATGAATTTTCAATGAGTGCTTCAAGCATTCTTAAAT
CTCGTTCGTTAATCAAACGTTTAGATCAATCTGAAATGGTGAAATTAGCGGAAGAAGCTTTAAATAAATCTACAGCAGAA
GAAGTTGTAGAATTAGTTGAAAAATATACTGCAGAATAA

Upstream 100 bases:

>100_bases
GTAAAGGCGCTGACATTACTATCTACACTGAAGGTAGCGATGAAAAAGAAGCAATTGAAGGACTAACTGAAGTTCTTAAG
AAAGAAGGATTGGCTGAATA

Downstream 100 bases:

>100_bases
TATCTGAATAAAGACCTAGACAATTTTGTTGTCTAGGTCTTTTTATGTTGTGAAATAGCCTCCGCCCAAGTATAATTGGA
AATACAGTTAGTTTTAAAAG

Product: phosphotransferase system enzyme I

Products: NA

Alternate protein names: Phosphotransferase system, enzyme I [H]

Number of amino acids: Translated: 572; Mature: 571

Protein sequence:

>572_residues
MAKELKGIAASDGIAIAKAYLLVEPDLSYEKTEVTDVESEVKRFESALEVSRTELSMIREKAAKDLGEDKAQIFDAHLLV
LNDPELTGPIEESIKNSKTNAETALQETTDMFIGMFESMDNEYMRERAADIKDVRKRVLSHLLGVTIPNPALIDEEVVVV
AADLTPSDTAQLNRNFVKGFVTDIGGRTSHSAIMARSLEIPAVVGTKEVTASVAKNDIVIIDGLEGNVIIHPTEEQIAHY
EKIKSDFALQQAEWDKLKNEKTVSKDGVHVELAANIGTPNDLEGVISNGGEAVGLYRTEFLYMGRDDFPTEEEQFEAYKA
VVSGMDGKSVVVRTLDIGGDKTLPYLELPEEMNPFLGFRAIRLCFANEELFRTQLRALLRASVYGNLKIMFPMIATVNEF
RQARDILLDEKAKLKAAGTEVSDSIELGIMIEIPAAAVLADQFAKEVDFFSIGTNDLIQYTMAADRMNERVSYLYQPYNP
SILRLVKMVIDASHKEGKWTGMCGEMAGDQTAVPLLLGLGLDEFSMSASSILKSRSLIKRLDQSEMVKLAEEALNKSTAE
EVVELVEKYTAE

Sequences:

>Translated_572_residues
MAKELKGIAASDGIAIAKAYLLVEPDLSYEKTEVTDVESEVKRFESALEVSRTELSMIREKAAKDLGEDKAQIFDAHLLV
LNDPELTGPIEESIKNSKTNAETALQETTDMFIGMFESMDNEYMRERAADIKDVRKRVLSHLLGVTIPNPALIDEEVVVV
AADLTPSDTAQLNRNFVKGFVTDIGGRTSHSAIMARSLEIPAVVGTKEVTASVAKNDIVIIDGLEGNVIIHPTEEQIAHY
EKIKSDFALQQAEWDKLKNEKTVSKDGVHVELAANIGTPNDLEGVISNGGEAVGLYRTEFLYMGRDDFPTEEEQFEAYKA
VVSGMDGKSVVVRTLDIGGDKTLPYLELPEEMNPFLGFRAIRLCFANEELFRTQLRALLRASVYGNLKIMFPMIATVNEF
RQARDILLDEKAKLKAAGTEVSDSIELGIMIEIPAAAVLADQFAKEVDFFSIGTNDLIQYTMAADRMNERVSYLYQPYNP
SILRLVKMVIDASHKEGKWTGMCGEMAGDQTAVPLLLGLGLDEFSMSASSILKSRSLIKRLDQSEMVKLAEEALNKSTAE
EVVELVEKYTAE
>Mature_571_residues
AKELKGIAASDGIAIAKAYLLVEPDLSYEKTEVTDVESEVKRFESALEVSRTELSMIREKAAKDLGEDKAQIFDAHLLVL
NDPELTGPIEESIKNSKTNAETALQETTDMFIGMFESMDNEYMRERAADIKDVRKRVLSHLLGVTIPNPALIDEEVVVVA
ADLTPSDTAQLNRNFVKGFVTDIGGRTSHSAIMARSLEIPAVVGTKEVTASVAKNDIVIIDGLEGNVIIHPTEEQIAHYE
KIKSDFALQQAEWDKLKNEKTVSKDGVHVELAANIGTPNDLEGVISNGGEAVGLYRTEFLYMGRDDFPTEEEQFEAYKAV
VSGMDGKSVVVRTLDIGGDKTLPYLELPEEMNPFLGFRAIRLCFANEELFRTQLRALLRASVYGNLKIMFPMIATVNEFR
QARDILLDEKAKLKAAGTEVSDSIELGIMIEIPAAAVLADQFAKEVDFFSIGTNDLIQYTMAADRMNERVSYLYQPYNPS
ILRLVKMVIDASHKEGKWTGMCGEMAGDQTAVPLLLGLGLDEFSMSASSILKSRSLIKRLDQSEMVKLAEEALNKSTAEE
VVELVEKYTAE

Specific function: General (non sugar-specific) component of the phosphoenolpyruvate-dependent sugar phosphotransferase system (sugar PTS). This major carbohydrate active-transport system catalyzes the phosphorylation of incoming sugar substrates concomitantly with their tr

COG id: COG1080

COG function: function code G; Phosphoenolpyruvate-protein kinase (PTS system EI component in bacteria)

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the PEP-utilizing enzyme family [H]

Homologues:

Organism=Escherichia coli, GI1788756, Length=570, Percent_Identity=50.3508771929825, Blast_Score=560, Evalue=1e-161,
Organism=Escherichia coli, GI1788726, Length=575, Percent_Identity=36, Blast_Score=353, Evalue=2e-98,
Organism=Escherichia coli, GI48994992, Length=501, Percent_Identity=37.125748502994, Blast_Score=347, Evalue=1e-96,
Organism=Escherichia coli, GI1789193, Length=579, Percent_Identity=31.9516407599309, Blast_Score=300, Evalue=1e-82,
Organism=Escherichia coli, GI1787994, Length=423, Percent_Identity=26.7139479905437, Blast_Score=121, Evalue=1e-28,
Organism=Escherichia coli, GI226510935, Length=186, Percent_Identity=28.494623655914, Blast_Score=86, Evalue=9e-18,

Paralogues:

None

Copy number: 360 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 2659 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. 4,000 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR008279
- InterPro:   IPR006318
- InterPro:   IPR018274
- InterPro:   IPR023151
- InterPro:   IPR000121
- InterPro:   IPR008731
- InterPro:   IPR015813 [H]

Pfam domain/function: PF05524 PEP-utilisers_N; PF00391 PEP-utilizers; PF02896 PEP-utilizers_C [H]

EC number: =2.7.3.9 [H]

Molecular weight: Translated: 63214; Mature: 63083

Theoretical pI: Translated: 4.42; Mature: 4.42

Prosite motif: PS00370 PEP_ENZYMES_PHOS_SITE ; PS00742 PEP_ENZYMES_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
3.5 %Met     (Translated Protein)
3.8 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
3.3 %Met     (Mature Protein)
3.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAKELKGIAASDGIAIAKAYLLVEPDLSYEKTEVTDVESEVKRFESALEVSRTELSMIRE
CCCCCCCCCCCCCCEEEEEEEEECCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHH
KAAKDLGEDKAQIFDAHLLVLNDPELTGPIEESIKNSKTNAETALQETTDMFIGMFESMD
HHHHHCCCHHHHHEEEEEEEECCCCCCCCHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHH
NEYMRERAADIKDVRKRVLSHLLGVTIPNPALIDEEVVVVAADLTPSDTAQLNRNFVKGF
HHHHHHHHCCHHHHHHHHHHHHHCCCCCCCCCCCCCEEEEEECCCCCHHHHHHHHHHHHH
VTDIGGRTSHSAIMARSLEIPAVVGTKEVTASVAKNDIVIIDGLEGNVIIHPTEEQIAHY
HHHCCCCCCHHHHHHHCCCCCEEECCHHHHHHHCCCCEEEEECCCCCEEEECCHHHHHHH
EKIKSDFALQQAEWDKLKNEKTVSKDGVHVELAANIGTPNDLEGVISNGGEAVGLYRTEF
HHHHHHHHHHHHHHHHHCCCCCCCCCCEEEEEEECCCCCCCHHHHHCCCCCEEEEEEEEE
LYMGRDDFPTEEEQFEAYKAVVSGMDGKSVVVRTLDIGGDKTLPYLELPEEMNPFLGFRA
EEECCCCCCCCHHHHHHHHHHHHCCCCCEEEEEEEECCCCCCCCCEECCHHCCHHHHHHH
IRLCFANEELFRTQLRALLRASVYGNLKIMFPMIATVNEFRQARDILLDEKAKLKAAGTE
HHHHHCCHHHHHHHHHHHHHHHHHCCEEEEHHHHHHHHHHHHHHHHHHCCCHHHHHCCCC
VSDSIELGIMIEIPAAAVLADQFAKEVDFFSIGTNDLIQYTMAADRMNERVSYLYQPYNP
CCCCEEEEEEEECCHHHHHHHHHHHHCCEEECCCHHHHHHHHHHHHHHHHHHHHCCCCCH
SILRLVKMVIDASHKEGKWTGMCGEMAGDQTAVPLLLGLGLDEFSMSASSILKSRSLIKR
HHHHHHHHHHCCCCCCCCCCCCCHHHCCCCHHHHHHHHCCCHHHHHHHHHHHHHHHHHHH
LDQSEMVKLAEEALNKSTAEEVVELVEKYTAE
CCHHHHHHHHHHHHCCCHHHHHHHHHHHHCCC
>Mature Secondary Structure 
AKELKGIAASDGIAIAKAYLLVEPDLSYEKTEVTDVESEVKRFESALEVSRTELSMIRE
CCCCCCCCCCCCCEEEEEEEEECCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHH
KAAKDLGEDKAQIFDAHLLVLNDPELTGPIEESIKNSKTNAETALQETTDMFIGMFESMD
HHHHHCCCHHHHHEEEEEEEECCCCCCCCHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHH
NEYMRERAADIKDVRKRVLSHLLGVTIPNPALIDEEVVVVAADLTPSDTAQLNRNFVKGF
HHHHHHHHCCHHHHHHHHHHHHHCCCCCCCCCCCCCEEEEEECCCCCHHHHHHHHHHHHH
VTDIGGRTSHSAIMARSLEIPAVVGTKEVTASVAKNDIVIIDGLEGNVIIHPTEEQIAHY
HHHCCCCCCHHHHHHHCCCCCEEECCHHHHHHHCCCCEEEEECCCCCEEEECCHHHHHHH
EKIKSDFALQQAEWDKLKNEKTVSKDGVHVELAANIGTPNDLEGVISNGGEAVGLYRTEF
HHHHHHHHHHHHHHHHHCCCCCCCCCCEEEEEEECCCCCCCHHHHHCCCCCEEEEEEEEE
LYMGRDDFPTEEEQFEAYKAVVSGMDGKSVVVRTLDIGGDKTLPYLELPEEMNPFLGFRA
EEECCCCCCCCHHHHHHHHHHHHCCCCCEEEEEEEECCCCCCCCCEECCHHCCHHHHHHH
IRLCFANEELFRTQLRALLRASVYGNLKIMFPMIATVNEFRQARDILLDEKAKLKAAGTE
HHHHHCCHHHHHHHHHHHHHHHHHCCEEEEHHHHHHHHHHHHHHHHHHCCCHHHHHCCCC
VSDSIELGIMIEIPAAAVLADQFAKEVDFFSIGTNDLIQYTMAADRMNERVSYLYQPYNP
CCCCEEEEEEEECCHHHHHHHHHHHHCCEEECCCHHHHHHHHHHHHHHHHHHHHCCCCCH
SILRLVKMVIDASHKEGKWTGMCGEMAGDQTAVPLLLGLGLDEFSMSASSILKSRSLIKR
HHHHHHHHHHCCCCCCCCCCCCCHHHCCCCHHHHHHHHCCCHHHHHHHHHHHHHHHHHHH
LDQSEMVKLAEEALNKSTAEEVVELVEKYTAE
CCHHHHHHHHHHHHCCCHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11679669 [H]