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The map label for this gene is ogt [H]

Identifier: 226223614

GI number: 226223614

Start: 1027437

End: 1027916

Strand: Direct

Name: ogt [H]

Synonym: Lm4b_01016

Alternate gene names: 226223614

Gene position: 1027437-1027916 (Clockwise)

Preceding gene: 226223611

Following gene: 226223617

Centisome position: 35.27

GC content: 38.12

Gene sequence:

>480_bases
ATGCTAAAACAACAAAAATCATCGCCAGTGGGTGATTTATTTATAACTATAGATGAAAAGTGGATTAGGAATATTTCGTA
TGACGAGCCCAAAAATTGGGAACTTCTAGAAGGAACTATAATAGAAAAAGAACTTTTTCAAGCATTAACTATCCAACTAG
ATGACTATTTTGAAGGCAAGAGAGAGCATTTTGATTTGCCGGTGCTCCTTAAAGGAACCGATTTTCAACAAAAGGTTTGG
CAAGCATTGAGTGAAATTCCGTATGGGGTTGTTGTGAGTTATAAAGACATTGCGATTTCAGCAGGTAGCCCTAAAGCTGT
GCAAGCAGTAGGACAAGCGAATCGCGCTAACCCAATTCCGATTATTATCCCATGTCACAGATGCGTGAAAAGTAATGGAG
AACTTGGGGGCTATAACGGGGCGGATGTAGATAAGAAACAATATTTACTTGCATTAGAAAAAGGCTTGAGTTTAAGTTAA

Upstream 100 bases:

>100_bases
CAAAAAGTATCAAATATTAACAGGGCATGACAGCTAGTTGCGAATGGAAGAGGGAAGTGCTATCTTTAGAATAATAACTT
AAGGGGAGAGGAGCGACAGA

Downstream 100 bases:

>100_bases
CTCAAGCCTTTTTTATTACACTGTTACTTGTTCTGTTATAGTAATTTCTTTATTTTCGTTAAGGGTTGCAACAAGATGTT
TTGCTTCTGGTTGTTCAATT

Product: methylated-DNA-protein-cystein methyltransferase

Products: NA

Alternate protein names: 6-O-methylguanine-DNA methyltransferase; MGMT; O-6-methylguanine-DNA-alkyltransferase [H]

Number of amino acids: Translated: 159; Mature: 159

Protein sequence:

>159_residues
MLKQQKSSPVGDLFITIDEKWIRNISYDEPKNWELLEGTIIEKELFQALTIQLDDYFEGKREHFDLPVLLKGTDFQQKVW
QALSEIPYGVVVSYKDIAISAGSPKAVQAVGQANRANPIPIIIPCHRCVKSNGELGGYNGADVDKKQYLLALEKGLSLS

Sequences:

>Translated_159_residues
MLKQQKSSPVGDLFITIDEKWIRNISYDEPKNWELLEGTIIEKELFQALTIQLDDYFEGKREHFDLPVLLKGTDFQQKVW
QALSEIPYGVVVSYKDIAISAGSPKAVQAVGQANRANPIPIIIPCHRCVKSNGELGGYNGADVDKKQYLLALEKGLSLS
>Mature_159_residues
MLKQQKSSPVGDLFITIDEKWIRNISYDEPKNWELLEGTIIEKELFQALTIQLDDYFEGKREHFDLPVLLKGTDFQQKVW
QALSEIPYGVVVSYKDIAISAGSPKAVQAVGQANRANPIPIIIPCHRCVKSNGELGGYNGADVDKKQYLLALEKGLSLS

Specific function: Involved in the cellular defense against the biological effects of O6-methylguanine (O6-MeG) in DNA. Repairs alkylated guanine in DNA by stoichiometrically transferring the alkyl group at the O-6 position to a cysteine residue in the enzyme. This is a sui

COG id: COG0350

COG function: function code L; Methylated DNA-protein cysteine methyltransferase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the MGMT family [H]

Homologues:

Organism=Homo sapiens, GI197304670, Length=172, Percent_Identity=34.3023255813954, Blast_Score=83, Evalue=1e-16,
Organism=Escherichia coli, GI1787596, Length=170, Percent_Identity=35.8823529411765, Blast_Score=100, Evalue=8e-23,
Organism=Escherichia coli, GI1788542, Length=90, Percent_Identity=51.1111111111111, Blast_Score=91, Evalue=3e-20,
Organism=Caenorhabditis elegans, GI115533070, Length=79, Percent_Identity=41.7721518987342, Blast_Score=65, Evalue=1e-11,
Organism=Caenorhabditis elegans, GI115533068, Length=79, Percent_Identity=41.7721518987342, Blast_Score=65, Evalue=2e-11,
Organism=Saccharomyces cerevisiae, GI6320001, Length=91, Percent_Identity=47.2527472527472, Blast_Score=80, Evalue=2e-16,
Organism=Drosophila melanogaster, GI17137554, Length=109, Percent_Identity=40.3669724770642, Blast_Score=67, Evalue=6e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001497
- InterPro:   IPR014048
- InterPro:   IPR008332
- InterPro:   IPR011991 [H]

Pfam domain/function: PF01035 DNA_binding_1; PF02870 Methyltransf_1N [H]

EC number: =2.1.1.63 [H]

Molecular weight: Translated: 17807; Mature: 17807

Theoretical pI: Translated: 5.28; Mature: 5.28

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.3 %Cys     (Translated Protein)
0.6 %Met     (Translated Protein)
1.9 %Cys+Met (Translated Protein)
1.3 %Cys     (Mature Protein)
0.6 %Met     (Mature Protein)
1.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLKQQKSSPVGDLFITIDEKWIRNISYDEPKNWELLEGTIIEKELFQALTIQLDDYFEGK
CCCCCCCCCCCEEEEEECHHHHHCCCCCCCCCCEEECCEEHHHHHHHHHHHHHHHHCCCC
REHFDLPVLLKGTDFQQKVWQALSEIPYGVVVSYKDIAISAGSPKAVQAVGQANRANPIP
CCCCCCCEEEECCCHHHHHHHHHHHCCCEEEEEEEEEEEECCCCHHHHHHHCCCCCCCCE
IIIPCHRCVKSNGELGGYNGADVDKKQYLLALEKGLSLS
EEEECHHHHCCCCCCCCCCCCCCCHHHHHHHHHCCCCCC
>Mature Secondary Structure
MLKQQKSSPVGDLFITIDEKWIRNISYDEPKNWELLEGTIIEKELFQALTIQLDDYFEGK
CCCCCCCCCCCEEEEEECHHHHHCCCCCCCCCCEEECCEEHHHHHHHHHHHHHHHHCCCC
REHFDLPVLLKGTDFQQKVWQALSEIPYGVVVSYKDIAISAGSPKAVQAVGQANRANPIP
CCCCCCCEEEECCCHHHHHHHHHHHCCCEEEEEEEEEEEECCCCHHHHHHHCCCCCCCCE
IIIPCHRCVKSNGELGGYNGADVDKKQYLLALEKGLSLS
EEEECHHHHCCCCCCCCCCCCCCCHHHHHHHHHCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA