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The map label for this gene is yhfE [H]

Identifier: 226223600

GI number: 226223600

Start: 1014536

End: 1015588

Strand: Direct

Name: yhfE [H]

Synonym: Lm4b_01002

Alternate gene names: 226223600

Gene position: 1014536-1015588 (Clockwise)

Preceding gene: 226223598

Following gene: 226223601

Centisome position: 34.83

GC content: 37.99

Gene sequence:

>1053_bases
TTGTCATACGAACCAAATACAAAAGAAACAATGCAAAAAATTAAAGAATTAACATCTATCCCAAGCCCAACTGGCAATAC
AGGGAAAATCATCGAAAAACTGGCAAAAGATTTAGATGCTTCAAAAATCCCTTACCATTTAAACAACAAAGGTGGATTAA
TCGTAACGCTTCCTGGGAAAGACGACACGAAACACCGTATGCTAACCGCCCATGTGGATACGCTTGGTGCAATGGTTAAA
GAAATTAAAGCAGACGGACGATTACTTTTAACCTTAATTGGAGGCTACCGTTTTAACGCGATTGAAGGCGAATATTGTAC
CATTGAGACAAGTGATGGAGATTTGTATAGTGGCACCATATTAATGCATCAAACTTCTGTCCATGTCTATAAAGATGCTG
GGACTGCTGAGCGTAATGACAAAAATATGGAAGTTCGCTTAGATGTAAAAGCATTAGATGCAGACAAAGTTCGTGCGCTT
GGAATAGAAGTAGGGGATTTTGTTTCGTTTGATCCGCGCGTGCAAATTATTAATGATGAATATATCAAATCTCGCCATTT
GGATGATAAAGCAAGTGTCGCGATTTTATTACAACTAATCAATCATATTCACGAAAATAAACTGGAGCTTCCTCATACAA
CCCATTTCCTCATCTCTAATAATGAAGAAATTGGCTATGGTGGTAATTCTAACATTCCGTCTGAAACGGTGGAATATTTA
GCTGTCGACATGGGAGCCCTTGGTGACGGACAAACGTCTGATGAATACACTGTTTCTATTTGTGCAAAAGATGGTAGTGG
CCCGTATCACCTTGGTTTGCGCAAACATTTAGTGGAGCTTGCTAAAAGCAATAATATTGATTATAAAGTGGATATTTATC
CGTTTTATGCATCTGATGCGAGTGCGGCAATTAATGCTGGTAATGATATTGTCCATGGTTTGATTGGACCAGGTATTGAT
GCTAGCCATGCATATGAACGCACACACCGCGATTCACTTTACCATACAGAAAAATTAGTTTATGCATATTTATTTTCAAA
TATATTGAAATAG

Upstream 100 bases:

>100_bases
AAAGGACTAAATTGGGAAAAAAGTTAATCCTAAGAAATGCGCATATTCAAGATAAGCGCTATAATATAATAGAAGAAAGC
GAACTAAAGGAGGAATTTTT

Downstream 100 bases:

>100_bases
GAGGAATCCCATATGAATGTCCATGATTTTTCTGAAAAAGCAATGAATGGCAAAGAAATAGCTTTAAGTGATTATAAAGG
TAAGGTATTACTCATCGTGA

Product: glucanase and peptidase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 350; Mature: 349

Protein sequence:

>350_residues
MSYEPNTKETMQKIKELTSIPSPTGNTGKIIEKLAKDLDASKIPYHLNNKGGLIVTLPGKDDTKHRMLTAHVDTLGAMVK
EIKADGRLLLTLIGGYRFNAIEGEYCTIETSDGDLYSGTILMHQTSVHVYKDAGTAERNDKNMEVRLDVKALDADKVRAL
GIEVGDFVSFDPRVQIINDEYIKSRHLDDKASVAILLQLINHIHENKLELPHTTHFLISNNEEIGYGGNSNIPSETVEYL
AVDMGALGDGQTSDEYTVSICAKDGSGPYHLGLRKHLVELAKSNNIDYKVDIYPFYASDASAAINAGNDIVHGLIGPGID
ASHAYERTHRDSLYHTEKLVYAYLFSNILK

Sequences:

>Translated_350_residues
MSYEPNTKETMQKIKELTSIPSPTGNTGKIIEKLAKDLDASKIPYHLNNKGGLIVTLPGKDDTKHRMLTAHVDTLGAMVK
EIKADGRLLLTLIGGYRFNAIEGEYCTIETSDGDLYSGTILMHQTSVHVYKDAGTAERNDKNMEVRLDVKALDADKVRAL
GIEVGDFVSFDPRVQIINDEYIKSRHLDDKASVAILLQLINHIHENKLELPHTTHFLISNNEEIGYGGNSNIPSETVEYL
AVDMGALGDGQTSDEYTVSICAKDGSGPYHLGLRKHLVELAKSNNIDYKVDIYPFYASDASAAINAGNDIVHGLIGPGID
ASHAYERTHRDSLYHTEKLVYAYLFSNILK
>Mature_349_residues
SYEPNTKETMQKIKELTSIPSPTGNTGKIIEKLAKDLDASKIPYHLNNKGGLIVTLPGKDDTKHRMLTAHVDTLGAMVKE
IKADGRLLLTLIGGYRFNAIEGEYCTIETSDGDLYSGTILMHQTSVHVYKDAGTAERNDKNMEVRLDVKALDADKVRALG
IEVGDFVSFDPRVQIINDEYIKSRHLDDKASVAILLQLINHIHENKLELPHTTHFLISNNEEIGYGGNSNIPSETVEYLA
VDMGALGDGQTSDEYTVSICAKDGSGPYHLGLRKHLVELAKSNNIDYKVDIYPFYASDASAAINAGNDIVHGLIGPGIDA
SHAYERTHRDSLYHTEKLVYAYLFSNILK

Specific function: Unknown

COG id: COG1363

COG function: function code G; Cellulase M and related proteins

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the peptidase M42 family [H]

Homologues:

Organism=Escherichia coli, GI1788727, Length=165, Percent_Identity=26.6666666666667, Blast_Score=65, Evalue=8e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR008007 [H]

Pfam domain/function: PF05343 Peptidase_M42 [H]

EC number: NA

Molecular weight: Translated: 38660; Mature: 38529

Theoretical pI: Translated: 5.82; Mature: 5.82

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
2.0 %Met     (Translated Protein)
2.6 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
1.7 %Met     (Mature Protein)
2.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSYEPNTKETMQKIKELTSIPSPTGNTGKIIEKLAKDLDASKIPYHLNNKGGLIVTLPGK
CCCCCCHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHCCCCCCCEEECCCCCEEEEECCC
DDTKHRMLTAHVDTLGAMVKEIKADGRLLLTLIGGYRFNAIEGEYCTIETSDGDLYSGTI
CCCCCEEEEHHHHHHHHHHHHHCCCCEEEEEEECCEEEEEECCCEEEEECCCCCEEEEEE
LMHQTSVHVYKDAGTAERNDKNMEVRLDVKALDADKVRALGIEVGDFVSFDPRVQIINDE
EEEECEEEEEECCCCCCCCCCCEEEEEEEEECCCCCEEEEECCCCCCCCCCCEEEEECCH
YIKSRHLDDKASVAILLQLINHIHENKLELPHTTHFLISNNEEIGYGGNSNIPSETVEYL
HHHHCCCCCHHHHHHHHHHHHHHHHCCCCCCCCEEEEEECCCEECCCCCCCCCHHHHHHH
AVDMGALGDGQTSDEYTVSICAKDGSGPYHLGLRKHLVELAKSNNIDYKVDIYPFYASDA
HHHHCCCCCCCCCCCEEEEEEEECCCCCEEHHHHHHHHHHHHCCCCCEEEEEEEEECCCC
SAAINAGNDIVHGLIGPGIDASHAYERTHRDSLYHTEKLVYAYLFSNILK
CCEECCCCHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCC
>Mature Secondary Structure 
SYEPNTKETMQKIKELTSIPSPTGNTGKIIEKLAKDLDASKIPYHLNNKGGLIVTLPGK
CCCCCHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHCCCCCCCEEECCCCCEEEEECCC
DDTKHRMLTAHVDTLGAMVKEIKADGRLLLTLIGGYRFNAIEGEYCTIETSDGDLYSGTI
CCCCCEEEEHHHHHHHHHHHHHCCCCEEEEEEECCEEEEEECCCEEEEECCCCCEEEEEE
LMHQTSVHVYKDAGTAERNDKNMEVRLDVKALDADKVRALGIEVGDFVSFDPRVQIINDE
EEEECEEEEEECCCCCCCCCCCEEEEEEEEECCCCCEEEEECCCCCCCCCCCEEEEECCH
YIKSRHLDDKASVAILLQLINHIHENKLELPHTTHFLISNNEEIGYGGNSNIPSETVEYL
HHHHCCCCCHHHHHHHHHHHHHHHHCCCCCCCCEEEEEECCCEECCCCCCCCCHHHHHHH
AVDMGALGDGQTSDEYTVSICAKDGSGPYHLGLRKHLVELAKSNNIDYKVDIYPFYASDA
HHHHCCCCCCCCCCCEEEEEEEECCCCCEEHHHHHHHHHHHHCCCCCEEEEEEEEECCCC
SAAINAGNDIVHGLIGPGIDASHAYERTHRDSLYHTEKLVYAYLFSNILK
CCEECCCCHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9579061; 9384377 [H]