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The map label for this gene is rsbW

Identifier: 226223513

GI number: 226223513

Start: 930575

End: 931048

Strand: Direct

Name: rsbW

Synonym: Lm4b_00912

Alternate gene names: 226223513

Gene position: 930575-931048 (Clockwise)

Preceding gene: 226223512

Following gene: 226223514

Centisome position: 31.95

GC content: 36.71

Gene sequence:

>474_bases
ATGGCAACAATGCATGACAAAATTACATTACAACTTCCTGCCAAGCCTGAATATGTTAGTTTAGGTAGACTTTCATTATC
AGGAATTGCAAGTCGCGCAGGATTTTCTTATGAAGCAATTGAAGATTTGAAAATAGCCGTAAGTGAAGCCATCACTAATT
CTGTAAAGCACGCATTTAAAGGAGAAGATGATGGTGAGATTACAGTAGAATATCTTATTTATGAAGACAAATTAGAAGTT
CGTGTTTCTGATAATGGAACAAGCTTCGACTTAGAAACCCGTAAACAAGAAATTGGCCCATATGAGGTAGGGGAAGATGC
GGAGATGATGCGTATCGGAGGTCTAGGTTTATTTTTAATTGAAACATTAATGGATGACGTGAAACTTTATTATGATGAAG
GGGTTTCTGTCGTAATGACCAAATATATTAATGAAAAGCAGGTGGAGGAGAATGCCAAAAGTATCTCAACCTGA

Upstream 100 bases:

>100_bases
GAACTTGTCTTGTTTGGTTTAAGCGACCGACTTTTCCGATTGTTTGAAATCACAGGATTGTCAGATATCATTGAAATCAA
AAATGTAGAGGGTGAAATGA

Downstream 100 bases:

>100_bases
TAAAGAGGCGAAAGAAAAAGTCTATATATGGATTGCCGCTTACCAAGAAAATGGCGATCAAGACGCCCAATATAATTTAG
TTGTTCATTACAAAAACCTA

Product: serine-protein kinase RsbW

Products: NA

Alternate protein names: Anti-sigma-B factor; Sigma-B negative effector rsbW [H]

Number of amino acids: Translated: 157; Mature: 156

Protein sequence:

>157_residues
MATMHDKITLQLPAKPEYVSLGRLSLSGIASRAGFSYEAIEDLKIAVSEAITNSVKHAFKGEDDGEITVEYLIYEDKLEV
RVSDNGTSFDLETRKQEIGPYEVGEDAEMMRIGGLGLFLIETLMDDVKLYYDEGVSVVMTKYINEKQVEENAKSIST

Sequences:

>Translated_157_residues
MATMHDKITLQLPAKPEYVSLGRLSLSGIASRAGFSYEAIEDLKIAVSEAITNSVKHAFKGEDDGEITVEYLIYEDKLEV
RVSDNGTSFDLETRKQEIGPYEVGEDAEMMRIGGLGLFLIETLMDDVKLYYDEGVSVVMTKYINEKQVEENAKSIST
>Mature_156_residues
ATMHDKITLQLPAKPEYVSLGRLSLSGIASRAGFSYEAIEDLKIAVSEAITNSVKHAFKGEDDGEITVEYLIYEDKLEVR
VSDNGTSFDLETRKQEIGPYEVGEDAEMMRIGGLGLFLIETLMDDVKLYYDEGVSVVMTKYINEKQVEENAKSIST

Specific function: Negative regulator of sigma-B activity. Phosphorylates and inactivates its specific antagonist protein, rsbV. Upon phosphorylation of rsbV, rsbW is released and binds to sigma-B, thereby blocking its ability to form an RNA polymerase holoenzyme (E-sigma-B

COG id: COG2172

COG function: function code T; Anti-sigma regulatory factor (Ser/Thr protein kinase)

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the anti-sigma-factor family [H]

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR010193
- InterPro:   IPR003594 [H]

Pfam domain/function: PF02518 HATPase_c [H]

EC number: =2.7.11.1 [H]

Molecular weight: Translated: 17498; Mature: 17367

Theoretical pI: Translated: 4.22; Mature: 4.22

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
3.8 %Met     (Translated Protein)
3.8 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
3.2 %Met     (Mature Protein)
3.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MATMHDKITLQLPAKPEYVSLGRLSLSGIASRAGFSYEAIEDLKIAVSEAITNSVKHAFK
CCCCCCEEEEECCCCCCEEEECCEEHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHC
GEDDGEITVEYLIYEDKLEVRVSDNGTSFDLETRKQEIGPYEVGEDAEMMRIGGLGLFLI
CCCCCCEEEEEEEEECEEEEEECCCCCEEEHHHHHHHCCCCCCCCCCCEEEECCHHHHHH
ETLMDDVKLYYDEGVSVVMTKYINEKQVEENAKSIST
HHHHHHHHHHHCCCCCEEHHHHCCHHHHHHHHHHCCC
>Mature Secondary Structure 
ATMHDKITLQLPAKPEYVSLGRLSLSGIASRAGFSYEAIEDLKIAVSEAITNSVKHAFK
CCCCCEEEEECCCCCCEEEECCEEHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHC
GEDDGEITVEYLIYEDKLEVRVSDNGTSFDLETRKQEIGPYEVGEDAEMMRIGGLGLFLI
CCCCCCEEEEEEEEECEEEEEECCCCCEEEHHHHHHHCCCCCCCCCCCEEEECCHHHHHH
ETLMDDVKLYYDEGVSVVMTKYINEKQVEENAKSIST
HHHHHHHHHHHCCCCCEEHHHHCCHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11679669 [H]